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61.
Glutathione (GSH) abundantly presents in plant and affects plants growth and development. To justify if GSH affects copper (Cu)-induced corn production, photosynthesis (Pn) rate, and photosynthetically active radiation (PAR), chlorophyll (Chl) content and Chl fluorescence, relative water content (RWC) and yields were evaluated. Different Cu concentrations (0, 0.2, 1.5 and 3.0 ppm of Zn) with or without 100 µm of N-acetyl cysteine (NAC) were arranged as completely randomize design with 5 replications. Results showed that both NAC and Cu affected plant height and leaf numbers. Copper reduced Pn rate, and PAR and increased RWC but no effect was observed on Chl content and Chl fluorescence in leaves. On the other hand, NAC application increased Pn, PAR, Chl parameters regardless of Cu treatment. In the presence of 1.5 ppm of Cu, corn plants showed improve yield and cob length irrespective to NAC treatment. Taken together, this study suggests that NAC might improve some physiological functions in plants to enhance Cu-induced corn production. 相似文献
62.
Scherer SW Cheung J MacDonald JR Osborne LR Nakabayashi K Herbrick JA Carson AR Parker-Katiraee L Skaug J Khaja R Zhang J Hudek AK Li M Haddad M Duggan GE Fernandez BA Kanematsu E Gentles S Christopoulos CC Choufani S Kwasnicka D Zheng XH Lai Z Nusskern D Zhang Q Gu Z Lu F Zeesman S Nowaczyk MJ Teshima I Chitayat D Shuman C Weksberg R Zackai EH Grebe TA Cox SR Kirkpatrick SJ Rahman N Friedman JM Heng HH Pelicci PG Lo-Coco F Belloni E Shaffer LG Pober B Morton CC Gusella JF Bruns GA Korf BR 《Science (New York, N.Y.)》2003,300(5620):767-772
63.
In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes 总被引:1,自引:0,他引:1
Momena Khatun Mohammad Musharraf Uddin Bhuiyan Jalal Uddin Ahmed Aminul Haque Mohammad Bozlur Rahman Mohammed Shamsuddin 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(1):75-82
Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries of prepubertal (n = 31) and pubertal (n = 61) black Bengal goats obtained from a slaughterhouse. Nuclear maturation was evaluated upon aspiration and following IVM in TCM-199 (Earle''s salt with L-glutamine and sodium bicarbonate) for 27 h at 39℃ under 5% CO2 in humidified air. The oocytes retrieval and efficiency (mean ± SD) per prepubertal and pubertal goats were 5.2 ± 0.6 and 6.8 ± 0.6, and 77.3 ± 0.1% and 80.5 ± 0.6%, respectively. Anaphase I - telophase I stages differed significantly (7.3 ± 0.8 vs. 2.6 ± 0.2, p < 0.05) between the two groups of goats. After IVM, the percentages of metaphase II were significantly higher (66.3 vs. 60.3, p < 0.05) in pubertal goats than in their prepubertal counterparts. The percentages of normal in vitro fertilization (IVF) in Fert-Tyrode''s albumin lactate pyruvate of pubertal goat oocytes did not differ between Percoll and swim-up sperm separation methods (36.7 ± 0.9% vs. 32.7 ± 1.3%, p > 0.05). Furthermore, sperm capacitation by heparin alone or in combination with ionomycin did not lead to a significant increase in the normal fertilization rate (34.8 ± 1.7 vs. 32.2 ± 1.5%, respectively) in the oocytes of pubertal goats. In conclusion, the ovaries of pubertal black Bengal goats obtained from the slaughterhouse could be used for in vitro embryo production. However, further optimization of the IVM and IVF techniques are necessary for satisfactory in vitro embryo production. 相似文献
64.
Min‐Jung GU Md. Jahangir ALAM Seon‐Ho KIM Che‐Ok JEON Moon‐Baek CHANG Young‐Kyoon OH Sang‐Cheol LEE Sang‐Suk LEE 《Animal Science Journal》2011,82(5):663-672
Molecular diversity of methanogens in the rumen of Korean black goats was investigated with 16S rRNA gene clone libraries using methanogen‐specific primers. The libraries were composed of rumen fluid‐associated methanogens (FAM) and rumen particle‐associated methanogens (PAM) from rumen‐fistulated Korean black goats. Among the 141 clones of the FAM library, the sequences were mostly related to two phyla, the Methanobacteriaceae family (77.3%) and the Thermoplasmatales family (22.7%); and among the 68 clones of the PAM library, sequences were also mainly clustered in the two phyla, the Thermoplasmatales family (63.24%) and the Methanobacteriaceae family (35.29%). Most of the sequenced clones in the two libraries were closely related to uncultured methanogenic archaeon. Quantitative real‐time PCR revealed that PAM (8.97 log 10) had significantly higher (P < 0.01) density of methanogens by the methanogenic 16S rRNA gene copies than FAM (7.57 log 10). The two clone libraries also showed difference in Shannon index (FAM library 1.70 and PAM library 1.59) and Chao 1 estimator (FAM library 18 and PAM library 17 operational taxonomic units). Apparent differences found in the microbial community from the two 16S rRNA gene libraries could be a result of such factors as the chemical and physical nature of the target material surface, types or component of diets, the interaction between the methanogens and other microbes, and age of the experimental goats. 相似文献
65.
A. K. M. Zakir Hossain Md. Ali Asgar M. Alamgir Hossain Toshihiko Tosaki Hiroyuki Koyama Tetsuo Hara 《Soil Science and Plant Nutrition》2005,51(1):43-49
The effects of B and Ca treatments on root growth, nutrient localization and cell wall properties in wheat ( Triticum aestivum L.) plants with and without Al stress were investigated. Seedlings were grown hydroponically in a complete nutrient solution for 7 d and then treated with B (0, 40 μM), Ca (0, 2,500 μM), and Al (0, 100 μM) in a 500 μM CaCl2 solution for 8 d. The cell wall materials (CWM) were extracted with a phenol: acetic acid: water (2:1:1 w/v/v) solution and used for subsequent pectin extraction with trans -1,2-diami-nocyclohexane- N,N,N,N -tetraacetic acid (CDTA) and Na2 CO3 solutions. Boron, Ca, and B + Ca treatments enhanced root growth by 19.5, 15.2, and 27.2%, respectively, compared to the control (pH 4.5). Calcium and B+Ca treatments enhanced root growth with Al stress by 43 and 54%, respectively, while B did not exert any effect. The amounts of CWM and pectin per unit of root fresh weight increased by Al treatment, whereas the Ca and B+Ca treatments slightly reduced the contents of these components. Seventy-four percent of total B, 69% of total Ca, and 85% of total Al were located in the cell wall in the B, Ca, and Al treatments, respectively and 32% of total B, 33% of total Ca, and 33% of total Al were located in the CDTA-soluble and Na2 CO3 -soluble pectin fractions. A more conspicuous localization of B was observed in the presence of Al. Aluminum treatment markedly decreased the Ca content in the cell wall as well as pectin fractions, mainly in the case of the CDTA-soluble pectin fraction. Boron + Ca treatment decreased the Al content in the cell wall and pectin fractions compared to the Ca treatment alone in the presence of Al. It is concluded that the B+Ca treatment enhanced root growth and, B and Ca uptake, and helped to maintain a normal B and Ca metabolism in the cell walls even in the presence of Al. 相似文献
66.
Alam Md. Zahangir Fakhru'l-Razi A. Abd-Aziz Suraini Molla Abul H. 《Water, air, and soil pollution》2003,149(1-4):113-126
The filamentous fungal strains such as Penicillium corylophilum (P), Aspergillus niger (A), Trichoderma harzianum (T) and Phanerochaete chrysosporium (PC) isolated from its relevant sources (wastewater, sewage sludge and sludge cake) were selected for compatible/incompatible mixed cultures. Six combinations of P. corylophilum and A. niger (P/A); P. corylophilum and P. chrysosporium (P/PC); P. corylophilum and T. harzianum (P/T); A. niger and T. harzianum (A/T); A. niger and P. chrysosporium (A/PC); T. harzianum and P. chrysosporium (T/PC) were used to evaluate their potential performance as compatible/incompatible mixed culture for the treatment of municipal wastewater sludge in a bioconversion process. The results of the present study showed that the combinations of P/A, P/PC and A/PC showed compatible growth and the rest of the combinations (P/T, A/T and T/PC) were incompatible cultures. A maximum production of dry biomass and dry filter cake were recorded in the compatible mixed culture of P. corylophilum and A. niger (P/A). A maximum reduction of COD (90%) and a decreased filtration time of treated sludge was observed in the case of P/A microbial mixed culture. The pH value was also affected by the fungal cultures. Effective results were observed by using microbial mixed culture after four days of treatment compared to other treatments (2 and 6 days). 相似文献
67.
Ichiyanagi T Shida Y Rahman MM Hatano Y Konishi T 《Journal of agricultural and food chemistry》2005,53(18):7312-7319
We previously determined that five rather hydrophobic metabolites appeared in blood plasma after oral administration of cyanidin 3-O-beta-D-glucopyranoside, but a group of hydrophilic metabolites still remained unidentified. In the present study, 12 hydrophilic metabolites found were collected from urine and plasma samples by high-performance liquid chromatography (HPLC) and then analyzed by tandem MS spectrometry. From the MS spectra, four metabolites out of 12 were assigned as glucuronides of cyanidin 3-O-beta-D-glucopyranoside and six out of 12 were glucuronides of the primary metabolites of cyanidin 3-O-beta-D-glucopyranoside (O-methyl cyanidin 3-O-beta-D-glucopyranoside). Extended glucuronides of cyanidin 3-O-beta-D-glucopyranoside and O-methyl cyanidin 3-O-beta-D-glucopyranoside showed their maximum plasma concentrations at 15 and 60 min (or 30 min) after oral administration, respectively. Their maximum plasma concentrations ranged from 15 to 70 nM. From the profile of urinary-excreted anthocyanins after intravenous administration, it was deduced that extended glucuronides of cyanidin 3-O-beta-D-glucopyranoside and O-methyl cyanidin 3-O-beta-D-glucopyranoside were mainly produced in the liver rather than by intestinal flora. The area under the plasma concentration curve was 0.25 micromol min/L for extended glucuronides of cyanidin 3-O-beta-D-glucopyranoside and 0.14 micromol min/L for O-methyl cyanidin 3-O-beta-D-glucopyranoside, respectively, when evaluated as cyanidin 3-O-beta-D-glucopyranoside equivalent, indicating that extended glucuronidation is a critical pathway in cyanidin 3-O-beta-D-glucopyranoside metabolism in rats. 相似文献
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