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21.
Craig A Shoemaker Chhorn Lim Mediha Yildirim-Aksoy Thomas L Welker & Phillip H Klesius 《Aquaculture Research》2006,37(12):1238-1245
This study determined the growth performance and acquired resistance of Nile tilapia, Oreochromis niloticus (L.) that survived Streptococcus iniae infection. Tilapia were challenged with three doses of S. iniae (8.8 × 103, 8.8 × 104 and 8.8 × 105 CFU fish?1 for low, medium and high challenges respectively). Groups of non‐injected and tryptic soy broth‐injected fish were maintained as controls. Significantly (P<0.05) higher mortality (45.0%) occurred in the high challenge treatment than in the low challenge treatment group (29.6%). The medium challenge group had mortality (36.3%) that did not differ significantly from the high or low treatment. Few fish died in the non‐injected and broth‐injected treatments (3.4% and 0.8% respectively). The tilapia that survived S. iniae infection used to assess growth performance were selected from survivors without gross clinical signs of disease. These fish were randomly stocked at a rate of 30 fish into each 57 L aquarium in triplicate and fed to apparent satiation for 8 weeks. No significant differences were detected in weight gain, feed intake, feed efficiency ratio or survival between S. iniae‐survived tilapia and the control treatments following the 8‐week growth performance trial. Following the 8‐week feeding study, tilapia were challenged with 1 × 106 CFU fish?1 of S. iniae to assess acquired immunity. Mean cumulative mortality was significantly higher (P<0.05) in the control treatments (41.7% for the non‐injected and 43.3% for the broth‐injected fish) than in the low, medium and high challenge treatments (7.4%, 3.3% and 8.3% respectively). Serum protein was significantly (P<0.05) elevated in the S. iniae‐survived tilapia that were subsequently challenged when compared with controls challenged for the first time. Agglutinating antibody titre was significantly higher in the fish in the medium and high challenge treatments, compared with the control fish challenged for the first time. The results suggest tilapia that survive S. iniae challenge without showing overt disease signs performed as well as non‐infected tilapia. Further, the S. iniae‐survived tilapia challenged following the 8‐week growth performance trial gained acquired resistance to homologous S. iniae challenge. 相似文献
22.
Ran Guo Chhorn Lim Hui Xia Mediha Yildirim Aksor Menghe Li 《Frontiers of Agriculture in China》2010,4(4):507-512
Six purified vitamin-free casein-based diets were formulated to contain six levels vitamin A at 0, 500, 1000, 2000, 4000,
and 8000 IU·kg−1, respectively. Tilapias (initial mean weight: 7.73±0.03 g) were fed the diets in quadruplicate aquaria to apparent satiation
twice daily for 10 weeks. No differences in mortality, weight gain, or feed efficiency ratio (FER) were observed among the
groups. Liver vitamin A levels reflected dietary vitamin A levels. Immune parameters, such as hemoglobin levels, total cell
count, red blood cell count, total serum protein, and serum lysozyme activity, did not vary with the dietary vitamin A levels.
White blood cell counts of fish in 2000 IU·kg−1 diet groups were significantly higher than that in other diets groups. Serum complement activities of fish in 2000 and 4000
IU·kg−1 vitamin A diet groups were also higher than those in other diet groups. After the 14-d challenge test, the mortality and
antibody titer were similar among the treatments. The results indicated that dietary vitamin A inclusions did not affect the
immune response of Oreochromis niloticus. 相似文献
23.
Frass is the by‐product of the larval meal industry and includes larval waste, exoskeleton sheds and residual feed ingredients. Experimental frass was derived from the larvae of black solder flies fed distillers' dried grains with solubles and had a protein and fat content of 216 and 60 g/kg, respectively. A 10‐week study was conducted to evaluate the effect of dietary levels of frass on growth, feed utilization, and body proximate and mineral composition of channel catfish, Ictalurus punctatus. Five diets containing 0, 50, 100, 200 and 300 g frass per kg diet were fed to channel catfish (5.24 ± 0.04 g) in quadruplicate aquaria to apparent satiation twice daily. Final weight gain was significantly increased in fish fed diets containing frass at levels from 100 to 300 g/kg. Fish fed diets without frass, and with 300 g/kg frass, showed the lowest and highest feed intake, respectively. Feed and protein efficiencies, however, were significantly lower in fish fed frass at levels of 200 g/kg and higher compared to the control diet. Survival, whole‐body composition and mineral content were not affected by frass. In summary, black soldier fly larval frass has potential as a protein source or just an ingredient for enhancing palatability of catfish diets. 相似文献
24.
Haitham H. Mohammed Taylor L. Brown Benjamin H. Beck Mediha Yildirim-Aksoy Rashida M. Eljack 《Journal Of Applied Aquaculture》2013,25(3):193-209
ABSTRACTWe evaluated a Saccharomyces cerevisiae fermentation product (Diamond V Original XPC) in hybrid catfish (Ictalurus furcatus x I. punctatus) for its potential effects on growth, blood parameters, and disease resistance. The trial featured four levels of inclusion that were added to a commercial 32% protein floating catfish ration. Following six weeks of feeding, we observed marginally heightened resistance to columnaris disease and saw significant changes in the levels of immune effectors in the serum, including lysozyme, complement, and immunoglobulin. 相似文献
25.
Mediha Yildirim-Aksoy Richard Shelby Chhorn Lim Phillip H. Klesius 《Journal of the World Aquaculture Society》2007,38(4):461-474
A 15‐wk study was conducted to evaluate the effect of supplemental menhaden fish oil levels and feeding duration on growth performance and tissue proximate and fatty acid (FA) compositions of juvenile channel catfish, Ictalurus punctatus. Dietary fish oil levels had no effect on final weight gain, feed efficiency, and survival of channel catfish. Tissue lipid contents were directly correlated to dietary lipid levels, while moisture contents were inversely related to dietary lipid levels. Fillet moisture contents progressively decreased, whereas fillet lipid increased with increasing feeding duration. Significant increase in saturated and total n‐3 FAs and decrease in monoenoic and total n‐6 FA in whole body and fillet were observed at each incremental level of dietary fish oil. Percentages of n‐3 and n‐3 highly unsaturated fatty acids in fillet of fish fed the control and 3% fish oil diets decreased with increasing feeding periods, whereas those of fish fed 6 or 9% added fish oil diets remained stable or increased. Ratios of n‐3/n‐6 were statistically comparable throughout the 15‐wk feeding. When expressed in terms of mg/g of fillet, the highest concentration of n‐3 was obtained in fillets of fish fed the 9% added fish oil diet for 15 wk. 相似文献