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101.
Zucchini squash (Cucurbita pepo L. cv. Green Magic) plants were grown hydroponically with nitrate (NO3):ammonium (NH4) ratio of 3:1 until the onset of flowering when the plants were assigned to four NO3:NH4 ratio (1:0, 1:1, 1:3, or 3:1) treatments. Changing the original nitrogen (N) form ratio significantly affected plant growth, fruit yield, nutrient element, and water uptake. Growth of plants was better when NO3‐N (1:0) was the sole form of N than when NH4‐N was part of the N treatment. Fruit yields for plants fertilized with 1:0 or 1:3 N‐form ratio were double those of plants grown continuously with 3:1 N ratio. The largest leaf area and plant water use were obtained with 1:0 N ratio treatment Total uptake of calcium (Ca), magnesium (Mg), and potassium (K) decreased with increasing NH4‐N proportion in the nutrient solution which suggest NH4‐N was competing with these cations for uptake. The results also demonstrated that growers may increase fruit yield by using a predominantly NO3‐N source fertilizer through the vegetative growth stage and by shifting the NO3:NH4 ratio during the reproductive phase.  相似文献   
102.
Brassica oleracea L. are important economic vegetables, and are capable of selenium (Se) enrichment to enhance human nutrition and health. Because Se enrichment may influence the nutrient balance of this crop, a study was done to test the effects of selenate‐Se on plant nutrients. Plants of a rapid‐cycling B. oleracea population were grown in nutrient solutions amended with Na2SeO4at 0.0, 3.0, 6.0, and 9.0 mg L‐1. Leaf tissue was then analyzed for nutrient content. Boron (B) (P=0.01), iron (Fe) (P=0.01), and phosphorus (P) (P=0.01) content decreased, while Se (P=0.01), sulfur (S) (P=0.01), and potassium (K) content (P=0.01) increased with increasing selenate‐Se treatments. Significant quadratic responses were found for magnesium (P=0.01) and molybdenum (P=0.01). No significant differences in leaf fresh or dry weight were detected. Changes in plant nutrient content can be expected when Brassicas are enhanced for delivery of beneficial organic Se.  相似文献   
103.
This study examined the 130 current full-text titles in the BioOne.1 and BioOne.2 collections to determine whether they are peer-reviewed, contain book reviews, and are supplied by document delivery vendors. We identified indexing coverage in 11 major databases in agriculture and life sciences: Aquatic Sciences and Fisheries Abstracts (ASFA), AGRICOLA, Biological Abstracts, BIOSIS Previews, CAB Abstracts, Current Contents, Environmental Sciences and Pollution Abstracts, GeoRef, Scopus, ISI Web of Science, and Zoological Record. We concluded that the majority of the titles in BioOne collections are refereed and scholarly, indexed in several of the major indexing and abstracting journals in the field of agriculture and life sciences, and available via several major document delivery vendors. We find both BioOne collections to be valuable, useful, cost-effective journal aggregations.  相似文献   
104.
105.
A multiplex PCR-ELISA protocol for detection ofClavibacter michiganensis subsp.sepedonicus (Cms) was developed that is based on primers for amplification of three single-copy, unique DNA sequences, Cms50, Cms72, and Cms85. The three sequences were simultaneously amplified from the genomes of all 42 strains of Cms that were tested including variant mucoid forms, but not from strains representing five related subspecies, andRathayibacter rathayi andRhodococcus faciens. The lowest limit of detection by gel electrophoresis was estimated to be approximately 300 CFU per mL when cells were spiked into potato core fluid, but sensitivity increases approximately 10-fold using PCR-ELISA. Inclusion of a sea anemone DNA fragment engineered so it could be amplified from the Cms72 primer set provided the simultaneous signal that the system functioned properly when any sample was free of the pathogen. The addition of hydrolyzed casein to the reaction mix was demonstrated to markedly reduce or eliminate inhibition of PCR by plant cell components or contaminants. Multiplex PCR-ELISA detection of Cms was determined to be verifiable for analysis of both stems and tubers based on the amplification of multiple sites in its genome, it provides absolute specificity, and it was more sensitive than detection based on gel electrophoresis of PCR products and serological approaches.  相似文献   
106.
107.
The effective use of manure nutrients requires an accurate assessment of their availability to the specific crops being grown. A fertilizer equivalence approach was used to evaluate the availability of nitrogen (N) and phosphorus (P) from liquid dairy manure to potatoes in field experiments conducted in northeast Wisconsin in 2000, 2001, and 2002. Crop responses to moderate and high rates of liquid dairy manure (93,500 and 187,000 L ha?1) were compared to results obtained from N or P fertilizer applied at five rates (0 to 269 kg ha?1). Availability estimates were made using the fertilizer equivalence method based on tuber yield, harvested tuber N or P concentration and uptake, petiole nitrate or total P concentration, and soil nitrate or extractable P phosphate levels. Results showed an apparent availability of manurial N from 10% to 40%, with an overall average across all 3 years and all parameters of 28.6% for the lower rate and 24.6% for the high rate. These values are slightly less than average availability measured where corn has been used as the test crop. Although all parameters did not show responses to P fertilizer additions in these trials, where estimates were possible, P availability ranged from 20% to 90% with an overall average across all 3 years of 55.4% for the low and 50.5% for the high manure rates. Despite potato being more shallow-rooted and somewhat less efficient in N and P use compared to corn, it appears that only minor adjustments to manurial nutrient availability estimates are warranted when potatoes are grown.  相似文献   
108.
The linear relationship between “site mean” and potato cultivar performance was used to develop predictive models for total yield, yield of US No 1 grade, per cent US No 1 grade, specific gravity and fried chip color. The best fitting linear expression for each of the traits was determined by linear regression for specific cultivars. Common limits for “site means” could then be substituted into the expressions for predictive purposes. Application of this method to large and small data sets demonstrated its use for interpreting preliminary yield trial information for the identification of superior breeding selections.  相似文献   
109.
110.
The behaviour of hens of two strains during the 10 min before laying in battery cages was studied. Hens of one strain typically exhibited backward-and-forward pacing before laying. Hens of the other strain did not pace and tended to sit. Individual hens of both strains were consistent in their expression of pacing and sitting before laying. There was genetic variation in the expression of both pacing and sitting before laying, and both traits responded to selection. Crosses between the two lines indicated additive genetic variation in the expression of sitting behaviour and non-additive variation in the expression of pacing behaviour.  相似文献   
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