Artificial insemination outcomes in beef females using bovine sperm with a detectable fertility-associated antigen     

Sprott LR  Harris MD  Forrest DW  Young J  Zhang HM  Oyarzo JN  Bellin ME  Ax RL 《Journal of animal science》2000,78(4):795-798

In this study, semen samples from 25 bulls that had passed a breeding soundness evaluation were analyzed for the presence or absence of a 31-kDa protein, known as fertility-associated antigen (FAA), on spermatozoal membranes. Eighteen bulls had FAA on sperm (FAA-positive) and seven were devoid of FAA on sperm (FAA-negative). A single ejaculate from each bull was extended and frozen with 25 to 30 x 10(6) sperm in .5-mL straws. Crossbred replacement heifers (n = 865) were estrus-synchronized and artificially inseminated either at timed AI or 12 h after they were detected in estrus. Mature cows (n = 285) were inseminated 12 h after they were detected in estrus during a 45-d AI period. Pregnancy rates (pooled) to first AI service for females (n = 764) inseminated with FAA-positive sperm were 65.6% and were 49.7% for females (n = 386) inseminated with FAA-negative sperm (P < .005). Among the estrus-synchronized replacement heifers, pregnancy rates to synchronized AI service for heifers (n = 550) inseminated with FAA-positive sperm were 62% and were 45.7% for heifers (n = 315) inseminated with FAA-negative sperm (P < .005). These data indicate that pregnancy rates to first AI service at spontaneous and synchronized estrus are higher when using semen from bulls with detectable FAA on spermatozoal membranes compared to semen from bulls devoid of FAA on membranes. Fertility-associated antigen is an important determinant for fertility potential of sperm from bulls to be used in AI breeding programs.  相似文献   

Regional brain monoamine concentrations and their alterations in bovine hypomagnesaemic tetany experimentally induced by a magnesium-deficient diet     

McCoy MA  Young PB  Hudson AJ  Davison G  Kennedy DG 《Research in veterinary science》2000,69(3):301-307

Monoamines are important brain neurotransmitters. An investigation was carried out to determine if hypomagnesaemic tetany was associated with alterations in regional brain monoamine concentrations in bovines. The results, established in cows with normal magnesium status, demonstrated that regional differences existed in the distribution and concentration of brain monoamines in the adult bovine, which were similar to those in other species. In magnesium-deficient cows, severe hypomagnesaemia and lowered cerebrospinal fluid (CSF) magnesium concentrations were associated with significant alterations in monoamine concentrations in some brain regions. Alterations in 3,4-dihydroxyphenylalanine (DOPA) and dihydroxyphenylacetic acid (DOPAC) concentrations in the corpus striatum, and dopamine (DA) in the cerebral cortex and cerebellum were recorded. These regions play an important role in both voluntary and involuntary motor function, and therefore these alterations may play a role in the aetiology of hypomagnesaemic tetany. However, there was no significant change in DA concentrations in the corpus striatum (the main dopaminergic region in the brain) associated with hypomagnesaemia. In addition, a significantly lower norepinephrine (NE) concentration in the corpus striatum of hypomagnesaemic animals was also recorded. Norephinephrine is generally excitatory and therefore lowered NE concentrations would be expected to result in depression rather than stimulation of motor function.  相似文献   

Genetic characterization of canine rotavirus isolated from a puppy in Korea and experimental reproduction of disease.   总被引：3，自引：0，他引：3  

Bo Kyu Kang  Dae Sub Song  Kwon Il Jung  Chul Seung Lee  Sung Jun Park  Jin Sik Oh  Dong Jun An  Jeong Sun Yang  Hyoung Joon Moon  Sang Sun Lee  Young Dhuk Yoon  Bong Kyun Park 《Journal of veterinary diagnostic investigation》2007,19(1):78-83

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Serologic and genetic characterization of North American H3N2 swine influenza A viruses          下载免费PDF全文

Marie Ren Gramer  Jee Hoon Lee  Young Ki Choi  Sagar M. Goyal    Han Soo Joo 《Canadian journal of veterinary research》2007,71(3):201-206

The H3N2 subtype of influenza A viruses isolated from pigs in the United States and Canada has shown both genetic and antigenic diversity. The objective of this study was to determine the serologic and genetic characteristics of contemporary strains of these viruses. Genetic analysis of 18 reference strains and 8 selected strains demonstrated differences in 1% to 9% of the nucleotides of the hemagglutinin (HA) gene. Phylogenetic analysis of the HA gene revealed 3 genetic clusters, as well as divergence of cluster III viruses from a cluster III prototype virus (A/Swine/Illinois/21587/99). By means of 1-way cross-hemagglutination inhibition with antiserum against 5 field isolates and 3 vaccine viruses, most of 97 isolates tested could be placed in 1 of 3 serogroups. The several isolates that did not react with any antiserum were in genetic cluster III, which suggests that continuous antigenic drift in cluster III may have resulted in virus variants. The efficacy of commercial vaccines against these virus variants should be evaluated with vaccination and challenge studies.  相似文献   

O型口蹄疫疫苗免疫牛抗体消长动态的LPB-ELISA检测     

魏孔福  祁淑芸林密 冯霞张峰  马军武 《中国兽医科技》2007,37(9):787-790

分别按一次免疫、首免后第15d加强免疫和首免后第60d加强免疫程序，对3组试验牛（每组牛20头）用O型口蹄疫疫苗进行了免疫。免疫后不同时间点用液相阻断ELISA（LPB-ELISA）测定了免疫牛O型口蹄疫抗体效价。结果，首免后第15d加强免疫牛和第60d加强免疫牛抗体水平较高，50％保护牛所占比例分别为56．3％和63．1％，完全受保护的牛所占比例分别为34．3％和29．5％。第60d加强免疫牛的保护率要高于第15d加强免疫牛和一次免疫牛的保护率。结果表明，首免后第60d加强免疫是最理想的免疫程序。  相似文献   

The expression and cellular localization of phospholipase D isozymes in the developing mouse testis     

Seungjoon Kim  Heechul Kim  Yongduk Lee  Jin Won Hyun  Young Ho Lee  Min Kyoung Shin  Do Sik Min  Taekyun Shin 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(3):209-212

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Effectiveness of 99mTc-tetrofosmin for assessment of heart functions in micropigs     

Min Young Lee  Sang Hun Lee  Jae Hong Park  Jung Sun Heo  Yu Jin Lee  Han Na Suh  Jung Jun Min  Young Soon Seo  Ho Jae Han 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(3):223-227

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Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice     

Sung Jae Shin  Seung Won Shin  Mi Lan Kang  Deog Yong Lee  Moon-Sik Yang  Yong-Suk Jang  Han Sang Yoo 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(4):383-392

We previously induced protective immune response by oral immunization with yeast expressing the ApxIIA antigen. The ApxI antigen is also an important factor in the protection against Actinobacillus pleuropneumoniae serotype 5 infection; therefore, the protective immunity in mice following oral immunization with Saccharomyces cerevisiae expressing either ApxIA (group C) or ApxIIA (group D) alone or both (group E) was compared with that in two control groups (group A and B). The immunogenicity of the rApxIA antigen derived from the yeast was confirmed by a high survival rate and an ApxIA-specific IgG antibody response (p < 0.01). The highest systemic (IgG) and local (IgA) humoral immune responses to ApxIA and ApxIIA were detected in group E after the third immunization (p < 0.05). The levels of IL-1β and IL-6 after challenge with an A. pleuropneumoniae field isolate did not change significantly in the vaccinated groups. The level of TNF-α increased in a time-dependent manner in group E but was not significantly different after the challenge. After the challenge, the mice in group E had a significantly lower infectious burden and a higher level of protection than the mice in the other groups (p < 0.05). The survival rate in each group was closely correlated to the immune response and histopathological observations in the lung following the challenge. These results suggested that immunity to the ApxIA antigen is required for optimal protection.  相似文献   

Note: Pyrethroid resistance and possible involvement of glutathione<Emphasis Type="Italic">S</Emphasis>-transferases in<Emphasis Type="Italic">Helicoverpa armigera</Emphasis> from Turkey     

Sakine Ugurlu  Metin Konus  Belgin Isgor  Mesude Iscan 《Phytoparasitica》2007,35(1):23-26

An introductory study was conducted to investigate the pyrethroid resistance ofHelicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) strains in Turkey, collected from cotton fields in the Adana and Antalya provinces, through two different synthetic pyrethroid insecticides: lambda-cyhalothrin and esfenvalerate. In addition, the roles of glutathioneS-transferases (GSTs) in this resistance mechanism were analyzed. It was found that whereas resistance ratios for lambda-cyhalothrin (LD₅₀ levels) were 3- and 98-fold increased in the Adana and Antalya strains, respectively, esfenvalerate ratios were 3.3- and 92.3-fold increased in the Adana and Antalya strains, respectively, with respect to the susceptible strain. Furthermore, Adana and Antalya strains showed 2.4- and 2.9-fold higher GST activities than the susceptible strain, respectively. In the Antalya field strain, the minor increase in GST activity compared with the resistance levels implies that GSTs may be not greatly involved in this resistance. It also provides evidence that they could not be the only metabolic mechanism responsible for resistance to lambda-cyhalothrin and esfenvalerate inH. armigera from Turkey. http://www.phytoparasitica.org posting Nov. 16, 2006.  相似文献   

Dhan Pal Singh and Arti Singh,Disease and Insect Resistance in Plants     

Christine Struck 《European journal of plant pathology / European Foundation for Plant Pathology》2007,118(1):91-92

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