Partial characterization of an atypical family I inorganic pyrophosphatase from cattle tick Rhipicephalus (Boophilus) microplus     

Costa EP  Campos E  de Andrade CP  Façanha AR  Saramago L  Masuda A  Vaz Ida S  Fernandez JH  Moraes J  Logullo C 《Veterinary parasitology》2012,184(2-4):238-247

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First report and molecular characterization of Trypanosoma vivax in cattle from state of Pernambuco, Brazil     

Pimentel Dde S  Ramos CA  Ramos RA  de Araújo FR  Borba ML  Faustino MA  Alves LC 《Veterinary parasitology》2012,185(2-4):286-289

This paper describes an outbreak of Trypanosoma vivax for the first time in the state of Pernambuco, Brazil, affecting dairy cattle in the municipality of Itambé in the northern coastal zone of the state. Clinical signs compatible with infection by blood protozoa and epidemic miscarriages were observed. The diagnosis of T. vivax was confirmed through biometric microscopy and molecular analysis with PCR and DNA sequencing. The T. vivax isolate detected in the present study proved to be genetically very close to other Brazilian isolates of the protozoan despite being geographically distant.  相似文献   

Saccharomyces boulardii reduces infection intensity of mice with toxocariasis     

de Avila LF  Conceição FR  Telmo Pde L  Dutra GF  de los Santos DG  Martins LH  Berne ME  da Silva PE  Scaini CJ 《Veterinary parasitology》2012,187(1-2):337-340

Several studies have shown the benefit of the use of probiotics in the prevention and treatment of diseases; however, few of them have investigated the effect of probiotics on parasitosis. In this study, the effect of Saccharomyces boulardii on the intensity of infection of mice with toxocariasis was evaluated. The animals were fed with a diet supplemented with S. boulardii for 15 days before inoculation with Toxocara canis eggs and for 2 or 60 days post-inoculation. S. boulardii promoted a reduction of approximately 36% in the average number of recovered T. canis larvae, suggesting that it can be used as an alternative to help control toxocariasis.  相似文献   

Humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A     

da Cunha IA  Zulpo DL  Bogado AL  de Barros LD  Taroda A  Igarashi M  Navarro IT  Garcia JL 《Veterinary parasitology》2012,186(3-4):216-221

We evaluated the humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A. The experiment used 13 mixed-breed pigs divided into the following three groups: G1 (vaccinated-challenged, n=6), which received the rhoptry vaccine (200(g/dose); G2 (adjuvant-challenged, n=4), which received PBS plus Quil-A; and G3 (unvaccinated-challenged, n=3), which was the control group. The treatments were performed intranasally at days 0, 21, and 42. Three pigs from G1 produced IgG and IgM antibody levels above the cut-off in the ELISA on the challenge day. Partial protection was observed in G1 at the chronic phase of infection when compared with G3. The preventable fractions were 41.6% and 6.5%, in G1 and G2, respectively. The results of this study suggest that rhoptry proteins plus Quil-A stimulated humoral, local, and systemic immune responses, which were able to partially protect the brain from cyst formation.  相似文献   

A new type of pterocarpanquinone that affects Toxoplasma gondii tachyzoites in vitro     

Portes Jde A  Netto CD  da Silva AJ  Costa PR  DaMatta RA  dos Santos TA  De Souza W  Seabra SH 《Veterinary parasitology》2012,186(3-4):261-269

Toxoplasma gondii, the agent of Toxoplasmosis, is an obligate intracellular protozoan able to infect a wide range of vertebrate cells, including nonprofessional and professional phagocytes. Therefore, drugs must have intracellular activities in order to control this parasite. The most common therapy for Toxoplasmosis is the combination of sulfadiazine and pyrimethamine. This treatment is associated with adverse reactions, thus, the development of new drugs is necessary. In previous studies, naphthoquinone derivatives showed anti-cancer activity functioning as agents capable of acting on groups of DNA, preventing cancer cells duplication. These derivatives also display anti-parasitic activity against Plasmodium falciparum and Leishmania amazonensis. The derivative pterocarpanquinone tested in this work resulted from the molecular hybridization between pterocarpans and naphtoquinone that presents anti-tumoral and anti-parasitic activities of lapachol. The aim of this work was to determine if this derivative is able to change T. gondii growth within LLC-MK2 cells. The drug did not arrest host cell growth, but was able to decrease the infection index of T. gondii with an IC(50) of 2.5 μM. Scanning and transmission electron microscopy analysis showed morphological changes of parasites including membrane damage. The parasite that survived tended to encyst as seen by Dolichos biflorus lectin staining and Bag-1 expression. These results suggest that pterocarpanquinones are drugs potentially important for the killing and encystment of T. gondii.  相似文献   

Enrofloxacin is able to control Toxoplasma gondii infection in both in vitro and in vivo experimental models     

Barbosa BF  Gomes AO  Ferro EA  Napolitano DR  Mineo JR  Silva NM 《Veterinary parasitology》2012,187(1-2):44-52

Currently, toxoplasmosis is treated with sulfadiazine and pyrimethamine. However, this treatment presents several adverse side effects; thus, there is a critical need for the development and evaluation of new drugs, which do not present the same problems of the standard therapy. Enrofloxacin is a fluoroquinolone antibiotic known to control infection against several bacteria in veterinary medicine. Recently, this drug has demonstrated protective effects against protozoan parasites such as Neospora caninum. The present study aimed to determine the effect of enrofloxacin in the control of Toxoplasma gondii infection. For this purpose, human foreskin fibroblast (HFF) cells were infected with T. gondii RH strain and treated with sulfadiazine, penicillin/streptomycin, pyrimethamine, or enrofloxacin. Following treatment, we analyzed the infection index, parasite intracellular proliferation and the number of plaques. Additionally, tissue parasitism and histological changes were investigated in the brain of Calomys callosus that were infected with T. gondii (ME49 strain) and treated with either sulfadiazine or enrofloxacin. Enrofloxacin was able to reduce the infection index, intracellular proliferation and the number of plaques in HFF cells infected by T. gondii in comparison with untreated or penicillin/streptomycin-treated ones. Enrofloxacin was more protective against T. gondii in HFF infected cells than sulfadiazine treatment (P<0.001). In addition, pyrimethamine, enrofloxacin or the associations of sulfadiazine plus pyrimethamine, enrofloxacin plus sulfadiazine or enrofloxacin plus pyrimethamine-treatments were able to reduce the plaque numbers in HFF cells infected by T. gondii when compared to medium, penicillin/streptomycin or sulfadiazine alone. In vivo experiments demonstrated that enrofloxacin diminished significantly the tissue parasitism as well as the inflammatory alterations in the brain of C. callosus infected with T. gondii when compared with untreated animals. Based on our findings, it can be concluded that enrofloxacin is a potential alternative drug for the treatment of toxoplasmosis.  相似文献   

Effect of vascularity of corpus luteum in progesterone production of mares     

Renata Cristina Uliani  Mateus José Sudano  Eunice Oba  Luciano Andrade Silva  Marco Antonio Alvarenga 《Journal of Equine Veterinary Science》2012

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Effects of lactoferrin supplementation to milk- and caseinate-based extenders on sperm motility,membrane integrity and nitric oxide levels of cooled stallion semen     

H.S. Martins  M.R. Souza  C.F.A.M. Penna  G.C. da Silva  S.F. Côrtes  R. Stahlberg  M.F. Brito  M.A. Lagares 《Journal of Equine Veterinary Science》2012

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Effect of nitric oxide enzyme (NOS) inhibition and effect of nitric oxide (NO) scavenger on motility patterns and hyperactivated population of cryopreserved equine sperm     

R.P. Arruda  D.F. Silva  A.F.C. Andrade  M.C. Caldas-Bussiere  M.A. Alonso  E.C.C. Celeghini  K.M. Lemes  H.F. Carvalho  S.A. Florez-Rodriguez 《Journal of Equine Veterinary Science》2012

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Haematic Mummification in a Mare With Twin Pregnancy     

Dietrich Pizzigatti  Fernando Arévalo Batista  Charles Ferreira Martins  Thiago Rinaldi Müller  Carlos Alberto Hussni 《Journal of Equine Veterinary Science》2012

Mummification occurs when the fetus dies during the second or third trimester of gestation and remains in the uterine cavity because of the persistence of the corpus luteum or existence of another live fetus. Generally, the mummified fetus and fetal membranes undergo desiccation. The hematic process is similar, but the fetus appears like melted chocolate and becomes lodged between the uterus and chorion. This report describes the treatment of dystocia in a mare with twin pregnancy, with one fetus having undergone hematic mummification. Although difficult to diagnose, the possibility of a second fetus should be investigated in mares with dystocia.  相似文献