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51.
Fujii M Otoi T Murakami M Tanaka M Une S Suzuki T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(3):305-307
Oocytes were recovered from bitch ovaries at various stages of the estrous cycle by the slicing method. The proportion of Grade A oocytes (darkly pigmented and surrounded in part, or whole, by dense layers of cumulus cells) were counted. Only Grade A oocytes were cultured in TCM199 supplemented with 5% fetal calf serum for evaluation of meiotic competence. There were no significant differences in the total number of oocytes or the proportion of Grade A oocytes that were recovered from bitches at various stages of the estrous cycle. Only 11% of the oocytes reached metaphase II (MII) at 72 hr after initiation of maturation culture. However, the proportions of oocytes reaching MII did not increase with culturing for up to 120 hr. 相似文献
52.
Regional variations in the distribution of small intestinal intraepithelial lymphocytes in three inbred strains of mice 总被引:2,自引:0,他引:2
Suzuki H Jeong KI Okutani T Doi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(8):881-887
The regional variation in the intraepithelial lymphocytes (IELs) in the small intestine was examined in BALB/c male and female mice and C3H/He and C57BL/6 male mice. The small intestines were taken from 11 to 12-week-old mice and divided equally into 3 parts (the proximal, middle and distal parts). IELs were isolated from each part of the intestine and analyzed with flow cytometer. The number of IELs was highest in the proximal part and lowest in the distal part. The distribution of IEL subsets was markedly different between the proximal and the distal parts, and that in the middle part showed the intermediate pattern. The percentage of alphabeta T cells were higher in the distal part. In alphabeta T cell subset, the percentage of CD8alphaalpha T cells was higher in the proximal part, whereas those of CD4 and CD4CD8alphaalpha double positive T cells were higher in the distal part. In gammadelta T cell subset, no regional variations were found. The regional variations in the number and subsets of IELs showed almost the same patterns between male and female BALB/c mice and similar patterns among three strains of mice. This strongly suggests that the regional variations in the small intestinal IELs are common to mouse species. 相似文献
53.
Tomonari Kasai Tomoko Suzuki Kozue Ono Ken'ichi Ogawa Yoshishige Inagaki Yuki Ichinose Kazuhiro Toyoda Tomonori Shiraishi 《Journal of General Plant Pathology》2006,72(5):265-272
We previously reported that the release of O2
− from isolated pea cell walls was enhanced by a 70-kDa glycoprotein elicitor but was suppressed by mucin-type glycopeptide
suppressors (supprescins A and B) prepared from pycnospore germination fluid of Mycosphaerella pinodes, causal agent of Mycosphaerella blight of pea. Here, we show that superoxide dismutase (SOD) in the apoplast fluid/cell wall
of pea seedlings responds to the fungal elicitor and suppressor molecules. In a pharmacological study and with internal amino
acid sequencing, the apoplastic SOD in a pea cultivar Midoriusui was found to be a Cu/Zn type SOD. We cloned a full-length
cDNA of the Cu/Zn-SOD and designated it as PsCu/Zn-SOD1. An increase in PsCu/Zn-SOD1 mRNA and the PsCu/Zn-SOD1 protein was induced by treatment with the elicitor more intensively
than by wounding. Such induction by the elicitor or wounding, however, was inhibited by the concomitant presence of supprescins.
The SOD activity of recombinant PsCu/Zn-SOD1 was regulated directly by these signal molecules in a manner similar to their
effect on the SOD activity in the apoplastic fluid and in the cell wall-bound proteins. Based on these findings, we discuss
a role for PsCu/Zn-SOD1 in the pea defense response.
The nucleotide sequence data of PsCu/Zn-SOD1 reported are available in the DDBJ/EMBL/GenBank databases under accession number AB189165. 相似文献
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Fluke antigens were detected in the ascitic fluid of mice infected with Fasciola gigantica. The precipitating antibodies reactive against the antigens from metacercariae were detected as early as 2 weeks after the infection, and those against the adult and immature worms appeared 3 and 4 weeks later. 相似文献
59.
M Suzuki K Sitizyo T Takeuchi T Saito 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(5):1077-1087
Electroencephalogram (EEG) examinations were carried out on three Japanese Black calves (1, 2 and 14 week old) affected with cerebrocortical necrosis (CCN) in order to investigate the relationship between EEG abnormalities and encephalic lesions. Some neurologic signs such as circling, astasia, convulsion and opisthotonus appeared in two of the calves (case 1 and 3). The other calf (case 2), however, showed no neurologic signs. The EEGs obtained from the two calves (case 1 and 2), in the awake state, showed constant high amplitude (50-160 microV) and slow (1-4 Hz) activity (HASA). One of these (case 1) had necrosis mainly in the right hemisphere and showed a markedly asymmetric sharp wave. On the other hand, in case 3, the EEG showed diffuse lowered activity, and almost flat in some leads. A decrease in fast activity observed in common with all three animals. These findings suggest that one of the characteristic EEG patterns of CCN patient may be HASA and decreased fast activity. Histopathologic diagnoses of the calves were CCN in various degrees. The degrees of abnormality of the EEGs seemed to accord with the seriousness of the lesions. Because the EEG obtained from the calf having no obvious neurologic signs showed an abnormal pattern, it was considered that EEG examinations in the early stages of disorders are valuable to diagnose CCN. 相似文献
60.
Nattaya Lourith Takeshi Katayama Kimiko Ishikawa Toshisada Suzuki 《Journal of Wood Science》2005,51(4):379-386
To investigate the biosynthesis and stereochemistry of syringylglycerol-8-O-4′-(sinapyl alcohol) ether (SGSE), a syringyl 8-O-4′ neolignan, feeding experiments and enzyme assays using Eucommia ulmoides were carried out. Diastereoselective formation of erythro-SGSE was found. When [8-14C]sinapyl alcohol was administered to excised shoots of E. ulmoides, 14C was incorporated into free SGSE and SGSE glucosides. In stems, incorporation into (+)-erythro-[14C]SGSE (0.037%) with 9.1% enantiomeric excess (% e.e.) was found; incorporation into the threo isomer was not detectable. Erythro-[14C]SGSE glucosides (0.047%) dominated over threo forms (0.007%) with 74.0% diastereomeric excess (% d.e.); both diastereomers were levorotatory with 32.0% e.e. and 18.3% e.e., respectively. In leaves, higher incorporation into (−)-erythro-[14C]SGSE (0.500%, 15.9% e.e.) than into the threo isomer (0.206%, 7.4% e.e.) was observed (41.6% d.e.). (−)-Erythro-[14C]SGSE glucosides (1.692%, 25.0% e.e.) were produced at higher rates than threo isomers (0.177%, 16.4% e.e.) with 81.0% d.e. In incubations of a mixture of [8-14C]sinapyl and [8-14C]coniferyl alcohols with an insoluble enzyme preparation from stems of E. ulmoides, erythro-SGSE was preferentially produced. The highest % d.e. (82.8) was observed at 60 min with the (+)-erythro isomer (21.4% e.e.) and the (−)-threo form (4.3% e.e.).Part of this report was presented at the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, April 2002, and the 47th Lignin Symposium, Fukuoka, October 2002 相似文献