Laparoscopic kidney biopsy in dogs: Comparison of cup forceps and core needle biopsy          下载免费PDF全文

Jiyoung Park  Jinwoo Lee  Hae‐Beom Lee  Seong Mok Jeong 《Veterinary surgery : VS》2017,46(2):226-232

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Suppression of pine wilt disease by an antibacterial agent,oxolinic acid     

Hyeok Ran Kwon  Gyung Ja Choi  Yong Ho Choi  Kyoung Soo Jang  Nack‐Do Sung  Mun Seong Kang  Yilseong Moon  Seung Kyu Lee  Jin‐Cheol Kim 《Pest management science》2010,66(6):634-639

BACKGROUND: Pine wilt disease (PWD) is very complex and has been reported to be caused by pine wood nematode, Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle, and its accompanying bacteria. However, there is no report on the control of PWD by antibacterial agent. The present study was performed to investigate disease control efficacy of antibacterial agents against PWD. RESULTS: Among six antibacterial antibiotics tested, oxolinic acid (OA) showed the strongest antibacterial activity against five bacteria isolated from three strains of pine wood nematode. In in vivo assay, it effectively suppressed the development of PWD in three‐year‐old seedlings of Pinus densiflora Sieb. & Zucc.; it showed 71% control when injected at 3 mg per seedling. A mixture of OA and the nematicidal agent abamectin (Ab) showed higher disease control efficacy against PWD than either OA or Ab alone. In addition, OA alone and a mixture of OA and Ab also controlled PWD in approximately 20‐year‐old pine trees under field conditions. CONCLUSION: This is the first report on the suppression of PWD by OA. The result strongly indicates that PWD could be controlled by antibacterial antibiotic alone and a combination of antibacterial and nematicidal agents. Copyright © 2010 Society of Chemical Industry  相似文献   

Anti-inflammatory effects of a Houttuynia cordata supercritical extract     

Sunhee Shin  Seong Soo Joo  Jeong Hee Jeon  Dongsun Park  Min-Jung Jang  Tae-Ook Kim  Hyun-Kyu Kim  Bang Yeon Hwang  Ki-Yon Kim  Yun-Bae Kim 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(3):273-275

Anti-inflammatory effects of Houttuynia cordata supercritical extract (HSE) were investigated in a carrageenan-air pouch model. HSE (200 mg/kg, oral) suppressed exudation and albumin leakage, as well as inflammatory cell infiltration. Dexamethasone (2 mg/kg, i.p.) only decreased exudation and cell infiltration, while indomethacin (2 mg/kg, i.p.) reduced exudate volume and albumin content. HSE lowered tumor-necrosis factor (TNF)-α and nitric oxide (NO), as well as prostaglandin E₂ (PGE₂). Dexamethasone only reduced TNF-α and NO, while indomethacin decreased TNF-α and PGE₂. The suppressive activity of HSE on NO and PGE₂ production was confirmed in RAW 264.7. These results demonstrate that HSE exerts anti-inflammatory effects by inhibiting both TNF-α-NO and cyclooxygenase II-PGE₂ pathways.  相似文献   

Rose (Rosa hybrida L.) EST-derived microsatellite markers and their transferability to strawberry (Fragaria spp.)     

Young Hoon Park  Seong Gyu Ahn  Young Mi Choi  Hye Jeong Oh  Dong Chun Ahn  Jin Gi Kim  Jum Soon Kang  Young Whan Choi  Byoung Ryong Jeong 《Scientia Horticulturae》2010

The ‘Genome database for Rosaceae (GDR)’ provides a large collection of expressed sequence tags (ESTs) harboring simple sequence repeats (SSRs) from several Rosaceae genera, including Rosa (rose). Primer pairs flanking SSR were designed for 312 unique Rosa ESTs based on GDR database. Eight rose (Rosa hybrida L.) genotypes were tested for PCR amplification, and 287 (92%) of the primer pairs generated allele-specific PCR bands that were readily scored. From 183 (63.7%) primer pairs that evidenced polymorphic alleles among the eight rose cultivars, 20 pairs evidencing EST sequence homology to known gene functions and high levels of polymorphism were selected and utilized for DNA fingerprinting and genetic diversity assessments of 47 rose hybrids. A total of 202 polymorphic bands were scored and generated unique fingerprints for each rose hybrid. The Nei–Li genetic similarity coefficients among 1081 pair-wise comparisons of 47 cultivars exhibited a broad range of genetic variations from 0.30 (‘Grand King’ and ‘Carnival’) to 0.99 (‘First Red’ and ‘Red Champ’). UPGMA cluster analysis divided 47 hybrids into five major groups and two sub-groups. The cross-species transferability of 273 EST-SSR primer pairs was evaluated using four genotypes of the strawberry, a genus member of the Rosaceae family. PCRs on the DNA samples of strawberry were successful for 165 primer pairs; among these, 123 pairs amplified 243 polymorphic bands. As surrogates of the marker transfer, the phenetic relationship among the four strawberry genotypes was evaluated. Genetic similarity coefficients varied from 0.78 (‘Maehyang’ and ‘Janghyee’) to 0.64 (‘Janghyee’ and ‘Pragana’). The results of cluster analysis showed that the three octaploid strawberry cultivars were quite similar, whereas the diploid ‘Pragana’ was related distantly at the genomic DNA level. The EST-SSR markers developed in the present study can be efficiently utilized for genetic diversity studies in Rosaceae.  相似文献   

花生种子挤压破碎机理的试验研究     

吕小莲  胡志超  于向涛  刘敏基  于昭洋 《华南农业大学学报》2013,34(2):262-266

为获取花生种子挤压破碎特性,降低对其机械化作业时的损伤,对花生主产区典型品种的花生种子进行了挤压破碎试验,分析了不同品种、不同含水率、不同挤压位置下花生种子的挤压破碎性能.试验结果表明:花生种子在不同含水率、不同挤压位置下所能承受的最大挤压力均有所不同,最终随含水率增加而降低,而在不同挤压位置条件下,同一含水率的种子所能承受的挤压力由大到小依次为腹面、侧面、顶面;不同品种花生种子在同一挤压位置下抗挤压破裂能力也有所不同,其原因均与种子自身的内部组成、外形尺寸等因素有关.  相似文献   

Preparation of regenerated cellulose fiber via carbonation. I. Carbonation and dissolution in an aqueous NaOH solution     

Sang?Youn?Oh  Dong?Il?YooEmail author  Younsook?Shin  Wha?Seop?Lee  Seong?Mu?Jo 《Fibers and Polymers》2002,3(1):1-7

Cellulose carbonate was prepared by the reaction of cellulose pulp and CO₂ with treatment reagents, such as aqueous ZnCl₂ (20–40 wt%) solution, acetone or ethyl acetate, at −5–0°C and 30–40 bar (CO₂) for 2 hr. Among the treatment reagents, ethyl acetate was the most effective. Cellulose carbonate was dissolved in 10% sodium hydroxide solution containing zinc oxide up to 3 wt% at −5–0°C. Intrinsic viscosities of raw cellulose and cellulose carbonate were measured with an Ubbelohde viscometer using 0.5 M cupriethylenediamine hydroxide (cuen) as a solvent at 20°C according to ASTM D1795 method. The molecular weight of cellulose was rarely changed by carbonation. Solubility of cellulose carbonate was tested by optical microscopic observation, UV absorbance and viscosity measurement. Phase diagram of cellulose carbonate was obtained by combining the results of solubility evaluation. Maximum concentration of cellulose carbonate for soluble zone was increased with increasing zinc oxide content. Cellulose carbonate solution in good soluble zone was transparent and showed the lowest absorbance and the highest viscosity. The cellulose carbonate and its solution were stable in refrigerator (−5°C and atmospheric pressure).  相似文献   

Improvement of mechanical and electrical properties of poly(ethylene glycol) and cyanoresin based polymer electrolytes     

Kyung Wha Oh  Ji Hyoung Choi  Seong Hun Kim 《Fibers and Polymers》2006,7(2):89-94

Ionic conductivity and mechanical properties of a mixed polymer matrix consisting of poly(ethylene glycol) (PEG) and cyanoresin type M (CRM) with various lithium salts and plasticizer were examined. The CRM used was a copolymer of cyanoethyl pullulan and cyanoethyl poly(vinyl alcohol) with a molar ratio of 1:1, mixed plasticizer was ethylene carbonate (EC) and propylene carbonate (PC) at a volume ratio of 1:1. The conductive behavior of polymer electrolytes in the temperature range of 298∼338 K was investigated. The PEG/LiClO₄ complexes exhibited the highest ionic conductivity of ∼10⁻⁵ S/cm at 25°C with the salt concentration of 1.5 M. In addition, the plasticized PEG/LiClO₄ complexes exhibited improvement of ionic conductivity. However, their complexes showed decreased mechanical properties. The improvement of ionic conductivity and mechanical properties could be obtained from the polymer electrolytes by using CRM. The highest ionic conductivity of PEG/CRM/LiClO₄/(EC-PC) was 5.33×10⁻⁴ S/cm at 25°C.  相似文献   

Effect of conformation on the properties of uniaxially drawn polylacide films upon drawing temperature     

Mi Ok Oh  Seong Hun Kim 《Fibers and Polymers》2016,17(7):992-999

Polylactide(PLA) films were drawn at various drawing temperature of 65, 90 and 120 °C. The effects of drawing temperature on structural conformation and properties of PLA films were investigated. It was confirmed that the PLA films at drawing temperature of 65 and 90 °C were composed of α′ phase crystal form. The strain-induced crystallization and molecular orientation increased with increasing the draw ratio, which result in improving the mechanical and thermal properties of α′ phase PLA films. However, at drawing temperature of 120 °C, the strain-induced crystallization and molecular orientation of PLA films were not distinctly detected. It was supposed that the rate of the chain relaxation was faster than chain orientation and strain-induced crystallization during uniaxial drawing process.  相似文献   

Processing of microcellular nanocomposite foams by using a supercritical fluid     

Dongho?Wee  Dong?Gi?Seong  Jae?Ryoun?YounEmail author 《Fibers and Polymers》2004,5(2):160-169

Polystyrene/layered silicate nanocomposites were prepared by melt intercalation. To examine the distribution of the clay in polymer matrix, small angle X-ray scattering (SAXS) and transmission electron microscopy (TEM) were used. Intercalated nanocomposites were obtained and their rheological properties were investigated. Microcellular nanocomposite foams were produced by using a supercritical fluid. As clay contents increased, the cell size decreased and the cell density increased. It was found that layered silicates could operate as heterogeneous nucleation sites. As the saturation pressure increased and the saturation temperature decreased, the cell size decreased and the cell density increased. Microcellular foams have different morphology depending upon the dispersion state of nanoclays.  相似文献   

Differential effects of treadmill exercise on calretinin immunoreactivity in type 2 diabetic rats in early and chronic diabetic stages     

Nam SM  Hwang IK  Yi SS  Yoo KY  Park OK  Yan B  Song W  Won MH  Yoon YS  Seong JK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):1037-1042

In this study, we investigated the effects of treadmill exercise on calretinin (CR), a marker of early postmitotic neurons, immunoreactivity in the dentate gyrus (DG) of Zucker diabetic fatty (ZDF) rats, before or after diabetes onset, and Zucker lean control (ZLC) rats. For this study, 6-week-old ZLC and prediabetic ZDF rats, and 22-week-old ZLC and ZDF rats were exercised on the treadmill. Sedentary ZLC and ZDF rats of the same age were used as exercise experiment controls. The exercised prediabetic ZDF rats did not show diabetes onset, while the sedentary prediabetic ZDF rats showed significantly increased blood glucose levels. The exercised diabetic ZDF rats exhibited a decrease in their blood glucose levels compared to the sedentary diabetic ZDF rats, but the levels were still above 20 mmol/l. ZLC rats of both ages were in the normoglycemic range. CR immunoreactivity was detected throughout the DG, including the subgranular zone and the polymorphic layer. Diabetic rats exhibited a significant decrease in the number of CR-immunoreactive cells and fibers in the DG. Exercise in the prediabetic ZDF rats significantly increased the number of CR-immunoreactive cells and fibers in the subgranular zone of the DG. In the ZLC and ZDF rats of chronic diabetic phase, exercise increased CR-immunoreactive neurons in the hilar region. These results suggest that diabetes significantly reduces the number of postmitotic CR-immunoreactive neurons and the intensity of immunoreactivity and that exercise increases these CR-related parameters in a diabetic stage-dependent manner.  相似文献