Serotyping of O and pilus antigens of Escherichia coli strains isolated from chickens with coli-septicemia.     

K Ike  K Kume  K Kawahara  H Danbara 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(5):1023-1027

One hundred and fifty one Escherichia coli strains were isolated from broiler chickens with coli-septicemia in Aichi (63 strains), Shizuoka (58 strains), and Kagoshima (30 strains) prefectures from 1980 to 1987, and their O and pilus antigens were serologically typed. One hundred and twenty five strains (82.8%) were typed into 23 O serogroups, and twenty six strains (17.2%) remained untypable. The predominant O serogroups were O2 (35 strains, 23.2%) and O78 (24 strains, 15.9%). Distribution of O serogroup was different, depending on prefectures where they are isolated. In total, 109 strains (72.2%) possessed Type 1 and/or Fmsha pili (Type 1; 41 strains, Fmsha; 22 strains, and Type 1 and Fmsha; 46 strains), and 42 strains (27.2%) were non-piliated. All the strains lacked K88, K99, 987P, F41, and Att25 pili. The ratios of piliated strains to non-piliated ones were almost the same among the three prefectures. Strains possessing Type 1 pili showed variety of O antigens, but most of the strains with Fmsha pili belonged to O2 serogroup.  相似文献   

Feeding habits of Pacific pomfret Brama japonica inthe transition zone of the central North Pacific     

HIKARU  WATANABE  TSUNEMI  KUBODERA  SHIGEYUKI  Kawahara 《Fisheries Science》2003,69(2):269-276

ABSTRACT:   We examined the diets of three size groups of the Pacific pomfret Bramajaponica collected in April and May 2000 in the transition zoneof the central North Pacific. Amphipods, cephalopods and fish werethree of the most common prey groups of pomfret. Of the three size groups,small pomfret (11.0–19.9 cm standard length (SL))preyed mainly on amphipods and occasionally on cephalopods (mainlytransitional species Onychoteuthis borealijaponica ) and fish.(These prey amphipods and fish could not be identified to lowertaxonomic levels because they were too digested.) Medium and largepomfret (20.0–30.9 and 31.0–40.9 cm SL,respectively) preyed mainly on cephalopods (mainly O. borealijaponica ,but also on two subarctic species, namely Berryteuthis anonychus and Gonatopsisborealis ), suggesting that these cephalopod species ensure energyfor the northward migration of these two size groups in the transitionzone in the spring. The next most common prey for medium pomfretwas amphipods (mainly subtropical species Phronima sedentaria and Platyscelusovoides ), followed by fish (mainly Bathylagus sp.). However,in the case of large pomfret, fish (mainly Paralepididae sp. 'A')were the next most common prey, followed by amphipods (mainly P.sedentaria and P. ovoides ), indicating that the importanceof fish as prey increases with growth for medium and large pomfret.  相似文献   

Allele‐specific polymerase chain reaction typing and sequencing of mitochondrial D‐loop region in broiler chickens in Japan          下载免费PDF全文

Takashi Harumi  Eiji Kobayashi  Mitsuru Naito 《Animal Science Journal》2015,86(9):815-817

This study aimed to comprehend a feature of single nucleotide polymorphism (SNP) in mitochondrial DNA (mtDNA) mainly of general broiler chickens in Japan. We typed two SNP sites (199C/T and 792A/G) of the D‐loop region in mtDNA by allele‐specific PCR (AS‐PCR) in 359 broiler (182 chunky and 177 cobb) and 506 layer (233 White Leghorn, 140 Barred Plymouth Rock and 133 Rhode Island Red) chickens. The SNP of 199C or 792A by AS‐PCR was observed in the chunky and cobb chickens, and not in the layers. The haplotype 199T/792G was observed in a part of cobb and all layers. By the result of AS‐PCR haplotyping and the broiler brands, the D‐loop region was sequenced in 44 broiler chickens (20 chunky and 24 cobb) and compared with the layers’ sequence data. Among the broiler and layer chickens, 21 SNP sites (including one insertion) and 11 sequence haplotypes were observed. Haplotype variation or correspondence was observed in and between the broiler brands. This study provides important information to establish a chicken meat traceability system by SNP haplotyping of mtDNA in Japan.  相似文献   

Irradiation history of Itokawa regolith material deduced from noble gases in the Hayabusa samples     

Nagao K  Okazaki R  Nakamura T  Miura YN  Osawa T  Bajo K  Matsuda S  Ebihara M  Ireland TR  Kitajima F  Naraoka H  Noguchi T  Tsuchiyama A  Yurimoto H  Zolensky ME  Uesugi M  Shirai K  Abe M  Yada T  Ishibashi Y  Fujimura A  Mukai T  Ueno M  Okada T  Yoshikawa M  Kawaguchi J 《Science (New York, N.Y.)》2011,333(6046):1128-1131

Noble gas isotopes were measured in three rocky grains from asteroid Itokawa to elucidate a history of irradiation from cosmic rays and solar wind on its surface. Large amounts of solar helium (He), neon (Ne), and argon (Ar) trapped in various depths in the grains were observed, which can be explained by multiple implantations of solar wind particles into the grains, combined with preferential He loss caused by frictional wear of space-weathered rims on the grains. Short residence time of less than 8 million years was implied for the grains by an estimate on cosmic-ray-produced (21)Ne. Our results suggest that Itokawa is continuously losing its surface materials into space at a rate of tens of centimeters per million years. The lifetime of Itokawa should be much shorter than the age of our solar system.  相似文献   

N-Acetyl-d-Glucosamine-Binding Lectin in Acropora tenuis Attracts Specific Symbiodiniaceae Cell Culture Strains     

Ryota Takeuchi  Mitsuru Jimbo  Fumika Tanimoto  Mariko Iijima  Hiroshi Yamashita  Go Suzuki  Saki Harii  Yoshikatsu Nakano  Ko Yasumoto  Shugo Watabe 《Marine drugs》2021,19(3)

Many corals establish symbiosis with Symbiodiniaceae cells from surrounding environments, but very few Symbiodiniaceae cells exist in the water column. Given that the N-acetyl-d-glucosamine-binding lectin ActL attracts Symbiodiniaceae cells, we hypothesized that corals must attract Symbiodiniaceae cells using ActL to acquire them. Anti-ActL antibody inhibited acquisition of Symbiodiniaceae cells, and rearing seawater for juvenile Acropora tenuis contained ActL, suggesting that juvenile A. tenuis discharge ActL to attract these cells. Among eight Symbiodiniaceae cultured strains, ActL attracted NBRC102920 (Symbiodinium tridacnidorum) most strongly followed by CS-161 (Symbiodinium tridacnidorum), CCMP2556 (Durusdinium trenchii), and CCMP1633 (Breviolum sp.); however, it did not attract GTP-A6-Sy (Symbiodinium natans), CCMP421 (Effrenium voratum), FKM0207 (Fugacium sp.), and CS-156 (Fugacium sp.). Juvenile polyps of A. tenuis acquired limited Symbiodiniaceae cell strains, and the number of acquired Symbiodiniaceae cells in a polyp also differed from each other. The number of Symbiodiniaceae cells acquired by juvenile polyps of A. tenuis was correlated with the ActL chemotactic activity. Thus, ActL could be used to attract select Symbiodiniaceae cells and help Symbiodiniaceae cell acquisition in juvenile polyps of A. tenuis, facilitating establishment of symbiosis between A. tenuis and Symbiodiniaceae cells.  相似文献   

Purification and characterization of phytase induced in tomato roots under phosphorus-deficient conditions     

Minggang Li  Mitsuru Osaki  Mamoru Honma  Toshiaki Tadano 《Soil Science and Plant Nutrition》2013,59(1):179-190

Phytase (myo-inositol hexakisphosphate phosphohydrolase; EC 3.1.3.8) was purified from roots of tomato plants grown under phosphorus-deficient conditions using five purification schemes. The phytase was successfully separated from the major acid phosphatase to an electrophoretic homogeneity. The native molecular weight of this enzyme was estimated to be about 164 kD by Bio-Gel P-200 gel filtration. The molecular weight of the subunit on SDS-PAGE was approximately 82 kD, indicating that the native form of the enzyme was a homodimer. The isoelectric point of tomato phytase was about 5.5. The enzyme exhibited a high affinity for phytic acid (K _m = 38 μM), and was strongly inhibited by phosphate, molybdate and fluoride. Among other characteristics of tomato phytase, the pH and temperature optima were 4.3 and 45°C, respectively. Tomato phytase contained a fairly high concentration of aspartic, glutamic acid and glycine residues.  相似文献   

Isolation and characterization of a cDNA from Catharanthus roseus which is highly homologous with phosphate transporter     

Motoshi Kai  Yukiko Masuda  Yasuhiro Kikuchi  Mitsuru Osaki  Toshiaki Tadano 《Soil Science and Plant Nutrition》2013,59(1):227-235

The PIT1 gene which is highly homologous with phosphate transporter was isolated from Catharanthus roseus and analyzed. The cBNA PIT1 contained an open reading frame of 542 amino acids and its sequence showed a 31, 30, and 34% identity with the phosphate transporter of Saccharomyces cerevisiae (PBO84), Neurospora crassa (PHO-5), and Glomus versiforme (GvPT), respectively. Furthermore, the cDNA PIT1 encoded a highly hydrophobic protein with 12 putative membrane-spanning regions and contained a conserved amino acid sequence reported in the human glucose transporter super-family* S. cerevisiae strain DpU (pho84 knockout strain) was unable to grow on low phosphate (55 μM) medium (LP medium). Expression of the PIT1 cDNA enabled DpU to grow on LP medium. Northern hybridization analysis revealed that the PIT1 gene was expressed in roots, stems, and young whole plant of C. roseus, but not in leaves.  相似文献   

A hybrid swarm population of Pinus densiflora × P. sylvestris inferred from sequence analysis of chloroplast DNA and morphological characters     

Young Hee Joung  Jerry L. Hill  Jung Oh Hyun  Ding Mu  Juchun Luo  Do Hyung Lee  Takayuki Kawahara  Jeung Keun Suh  Mark S. Roh 《林业研究》2013,24(1):53-60

To confirm a hybrid swarm population of Pinus densiflora × P. sylvestris in Jilin, China, we used needles and seeds from P. densiflora, P. sylvestris, and P. densiflora × P. sylvestris collected from natural stands or experimental stations to study whether shoot apex morphology of 4- year old seedlings can be correlated with the sequence of a chloroplast DNA simple sequence repeat marker (cpDNA SSRs). Total genomic DNA was extracted and subjected to sequence analysis of the pine cpDNA SSR marker Pt15169. Results show that morphological characters from 4-year old seedlings did not correlate with sequence variants of this marker. Marker haplotypes from all P. sylvestris trees had a CTAT element that was absent from all sampled P. densiflora trees. However, both haplotype classes involving this insertion/deletion element were found in a P. densiflora × P. sylvestris population and its seedling progeny. It was concluded that the P. densiflora × P. sylvestris accessions sampled from Jilin, China resulted from bi-directional crosses, as evidenced by both species’ cpDNA haplotypes within the hybrid swarm population.  相似文献   

Moisture distribution of soaked rice grains observed by magnetic resonance imaging and physicochemical properties of cooked rice grains     

Akemi Katsumi Horigane  Keitaro Suzuki  Mitsuru Yoshida 《Journal of Cereal Science》2013

Moisture distribution in individual polished rice grains was observed during soaking by magnetic resonance (MR) imaging, and a nuclear magnetic resonance (NMR) signal intensity profile (SI-profile) was generated from the MR image. The water penetration pattern during soaking roughly showed dissimilar trends between different varieties of japonica and japonica-indica hybrid rice. NMR signal intensity at the completion of water absorption varied within each grain; high at the periphery and the central region and low in the area between the periphery and the central region. High moisture content within the central region is due to loosely packed starch granules. The SI-profile was congruent for grains of the same variety harvested in different regions and years and characterized a grain moisture distribution for each variety. Moisture distribution was compared using SI-profiles for varieties with different amylose contents and new varieties bred for specific end-uses in Japan. The NMR signal intensity, which is related to the moisture content, at the surface of soaked grain was negatively correlated to the grain amylose content. The NMR signal intensities at the surface of soaked grain negatively correlated with the overall hardness of the cooked rice grain as measured by the single-grain low-high compression test.  相似文献   

Combined Hepatocellular-Cholangiocarcinoma in a Yellow-Headed Amazon (Amazona oratrix)     

Anusha Hemamali TENNAKOON  Takeshi IZAWA  Daisuke FUJITA  Yuki DENDA  Eiko SETO  Hiroshi SASAI  Mitsuru KUWAMURA  Jyoji YAMATE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(11):1507-1510

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