Allelic segregation of sex‐linked microsatellite markers in Nile tilapia (Oreochromis niloticus) and validation of inheritance in YY population     

Nargis Sultana  Mohd Golam Quader Khan  Mostafa Ali Reza Hossain  Md Samsul Alam 《Aquaculture Research》2020,51(5):1923-1932

Stocking of all‐male fingerling produced by direct administration of male hormone 17‐α‐methyltestosterone is the most preferred method for present‐day aquaculture of the Nile tilapia Oreochromis niloticus. However, due to the growing concern of negative impact of steroid hormone in food fish, production of ‘genetically male’ tilapia, which depends on the concrete and thorough understanding of sex determination, has long been a scientific curiosity. The objective of the present study was to identify reliable sex‐linked markers and to evaluate the applicability of those markers in terms of monosex production approach. ‘XY’ neofemales were produced by using synthetic oestrogen and identified through selective breeding and progeny testing. Three females with progeny not deviating from 3:1 sex ratio (male:female) were designated as ‘XY’ neofemales and were used subsequently to produce putative YY progeny. Among the fifteen microsatellite markers tested, marker ARO172 was most informative in differentiating male and female genotypes. Twenty‐seven F₂ fish from three families were identified as putative YY males based on marker genotyping, and four of them were crossed to produce F₃ to validate marker association by progeny testing. The YY males produced 86%–100% male progeny indicating ARO172 a unique sex‐linked marker applicable in marker‐assisted selection.  相似文献   

Assessment of growth condition for a candidate probiotic, <Emphasis Type="Italic">Shewanella algae</Emphasis>, isolated from digestive system of a healthy juvenile <Emphasis Type="Italic">Penaeus monodon</Emphasis>     

Shahram Shakiba Zadeh  Che Roos Saad  Annie Christianus  Mohd Salleh Kamarudin  Kamaruzaman Sijam  Mariana Nor Shamsudin  Vasantha Kumari Neela 《Aquaculture International》2010,18(6):1017-1026

To conquer disease problem in shrimp industries, probiotic biocontrol is a well-known remedy now. The antagonistic ability of separated isolates from different parts of juvenile P. monodon was screened against shrimp Vibrio pathogens, V. parahaemolyticus and V. alginolyticus. The most antagonistic effect was observed for an isolate that primarily identified as Shewanella algae using conventional method followed by Biolog GN and GP microplates. Since adaptability to the host optimum cultural condition of the target organism is of the great importance, response surface methodology, with central composite design, was applied to assess log cell count response of S. algae in different incubation conditions. Therefore, four independent variables were assumed as: temperature (10–50°C), pH (6–10), NaCl concentration (0–50‰) and time (12–60 h). The coefficients of multiple determinations (R ²) for the responses log cell count of S. algae being 0.827. Temperature was the merely significant independent variable that affected the log cell count of the candidate probiotic. The candidate probiotic was revealed a reasonable growth response in quite wide range of temperature, pH and NaCl concentration in which the maximum levels were in same range of optimum shrimp culture.  相似文献   

Streptococcosis in red hybrid tilapia (Oreochromis niloticus) commercial farms in Malaysia     

Najiah Musa  Lee Seong Wei  Nadirah Musa  Ruhil H Hamdan  Lee Kok Leong  Wendy Wee  Mohd Nur Amal  Basiriah M Kutty  & Siti Zahrah Abdullah 《Aquaculture Research》2009,40(5):630-632

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