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A 5-year-old Oldenburg mare presented for poor performance, head-tossing and bucking when ridden. An abnormal respiratory noise was audible during exercise. Computed tomography (CT) revealed right laryngeal dysplasia; an absent cricothyroid articulation, abnormally shaped thyroid cartilage, underdeveloped cricopharyngeus muscle, rostral displacement of the palatopharyngeal arch and an oesophageal air column. In this case, it was unlikely that the laryngeal pathology was related to the horse's clinical signs. This case report provides a reference CT examination of laryngeal dysplasia, corroborating previous findings using magnetic resonance imaging, radiography, ultrasonography and post-mortem examination.  相似文献   
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Micropores are important to soil moisture retention and plant growth. Microporosity and pore size distribution were evaluated using mercury intrusion porosimetery on aggregates from 35-year-old experiments started in 1962 at Wooster (40.5 °N, 82 °W) and South Charleston (39.8 °N, 84 °W) in Ohio, USA and from three land use practices on Kolombangara (8 °S, 157 °E) in Solomon Islands. Tillage treatments in Ohio included: moldboard plowing (MP), chisel plowing (CP), and no-till (NT) with continuous corn. The land use treatments in Kolombangara included: natural forest (NF), traditional farming (TF) and topsoil removal (TR). Pore size measured in aggregates ranged from 0.2 to 100 μm in diameter. Median pore radius was significantly (P < 0.05) larger for NT than for MP and CP treatments at Wooster, but not at South Charleston. Tillage treatments had significant effect on the volume of both storage and residual pores for both sites in Ohio. Volume of storage and residual pores were higher for Wooster than South Charleston soil. At Kolombangara, the NF treatment had significantly larger median and peak pore radii, and microporosity than TF and TR treatments. There was, however, no significant difference among treatments in the volume of pore size distribution. These data support a recommendation for adoption of no-till or conservation tillage in soils of the temperate region, and of minimal disturbance and effective erosion control in soils of the tropics.  相似文献   
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Callus produced from leaves of a Ghanaian strain of the neem tree, Azadirachta indica A. Juss has been shown to produce the natural insecticide azadirachtin when grown in a defined medium. The azadirachtin was isolated by standard procedures of solvent partition and column chromatography monitored by supercritical fluid chromatography. Biological activity was monitored with antifeedant tests using the desert locust (Schistocerca gregaria Forsk.). The azadirachtin was identified by chromatography on three independent chromatographic systems (SFC, HPLC & TLC) and two thin-layer colour tests. It has 100% antifeedant activity at < 0.04mg litre?1. The yield of azadirachtin was 0.0007% based on dry weight of callus.  相似文献   
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OBJECTIVE: To identify the major allergenic proteins from the 3 main species of dust mites to which dogs react (Dermatophagoides farinae, D. pteronyssinus, and Euroglyphus maynei) and evaluate the potential cross-reactivity of dust mite allergens with antigens from the ectoparasitic mite Sarcoptes scabiei var canis. SAMPLE POPULATION: Sera from 83 dogs with atopic dermatitis. PROCEDURE: Sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting using serum from atopic dogs was used to identify IgE-binding proteins in extracts of the 4 mite species. RESULTS: Sera of atopic dogs contained IgE against 23, 17, 25, and 17 allergens from D. farinae, D. pteronyssinus, E. maynei, and S. scabiei, respectively. Unlike the situation for humans, the major allergens for dogs are mostly proteins that are larger than 90 kd molecular weight. Dermatophagoides farinae and E. maynei appear to be more allergenic for dogs than is D. pteronyssinus. Some dogs with serum IgE against dust mites also had IgE against antigens of S. scabiei var canis. CONCLUSIONS AND CLINICAL RELEVANCE: Multiple dust mite allergens induce an IgE response in dogs. These allergens are mostly greater than 90 kd molecular weight.  相似文献   
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Molecular and biological characterisation of Cryptosporidium in pigs   总被引:1,自引:0,他引:1  
OBJECTIVE: Genetic and biological characterisation of 12 isolates of Cryptosporidium from pigs and comparing them with Cryptosporidium isolates from humans and cattle. DESIGN: Cryptosporidium isolates from pigs were compared with those obtained from human and cattle using rDNA sequence analysis. The infectivity of two of the porcine isolates was determined in neonatal mice and the clinical history of the infected pigs recorded. RESULTS: Pig-derived isolates of Cryptosporidium exhibited two distinct genotypes; a porcine genotype and a bovine genotype, which is common to cattle and other livestock. The porcine genotype did not produce any infection in neonatal mice whereas the bovine genotype did. CONCLUSION: Two distinct genetically and biologically differing strains of Cryptosporidium appeared to be associated with acute diarrhoea in pigs. Whether Cryptosporidium was a primary or secondary pathogen is unclear but warrants further investigation. As the bovine genotype is known to infect humans, the results suggest that pigs can act as reservoirs of cryptosporidial infections for humans and other live-stock. The zoonotic potential of the pig-adapted genotype is uncertain and requires further study.  相似文献   
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