Functional scFv antibody sequences against the organophosphorus pesticide chlorpyrifos     

Alcocer MJ  Doyen C  Lee HA  Morgan MR 《Journal of agricultural and food chemistry》2000,48(2):335-337

Two functional single-chain Fv (scFv) antibodies that recognize specifically the widely used organophosphorus pesticide chlorpyrifos-ethyl were derived from two murine hybridoma cell lines. It is shown that the functional scFvs could be isolated without any rounds of selection, with a success rate dependent on the efficiency of amplification of the functional light chain gene family by the specific primers. Besides four new functional immunoglobulin variable gene sequences, the isolation of a new pseudogene is reported.  相似文献   

The influence of grass density on effectiveness of contour grass strips for control of soil erosion on low angle slopes     

N. Melville  R.P.C. Morgan 《Soil Use and Management》2001,17(4):278-281

Abstract. The effectiveness of contour grass strips in erosion control was investigated in a field experiment involving two grass treatments ( Festuca ovina and Poa pratensis ) and a bare soil control on an erodible sandy loam soil on a 5° slope using simulated rainstorms of 40 mm h^–1 for 45 minutes duration. The grass strips resulted in significantly ( P < 0.05) lower runoff and soil loss than the bare soil but there was no significant difference in the performance of the two grasses, despite their differences in density, height and leaf size. The effect of the lower density of the Poa pratensis was offset by its larger stem diameter so that the surface area facing the flow was similar for both grasses. Instead of acting as a filter with sedimentation occurring within the barrier, the grass strips operated by ponding water upslope of the barriers. Deposition then occurred in the ponded area above the barrier.  相似文献   

Editorial     

Byron J. T. Morgan 《Journal of Agricultural, Biological & Environmental Statistics》2005,10(4):391

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Aural haematomata, cauliflower ears and Psoroptes in sheep [corrected     

K L Morgan 《The Veterinary record》1991,128(19):459-460

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MAGNETIC RESONANCE IMAGING OF OCULAR AND ORBITAL DISEASE IN 5 DOGS AND A CAT     

Rhea V.  Morgan DVM  Rachel D.  Ring DVM  Daniel A.  Ward DVM  PhD  William H.  Adams DVM 《Veterinary radiology & ultrasound》1996,37(3):185-192

Magnetic resonance (MR) images were acquired in five dogs and one cat with ocular and orbital disease. MR images were obtained in the dorsal or oblique dorsal, and oblique sagittal planes. Pathologic changes identified in MR images included inflammatory lesions, cystic structures, and neoplasms. All abnormalities were readily apparent in TI-weighted images. MR findings in affected animals were often similar in signal intensity, location, and growth pattern to those found in people with comparable diseases. Although no MR changes were considered pathognomonic for a given disease, MR imaging provided detailed information on the homogeneity, extent and invasiveness of the lesions.  相似文献   

The Upper Gastrointestinal Tract in the Cat: A Protocol for Contrast Radiography     

J. P. Morgan 《Veterinary radiology & ultrasound》1977,18(5):134-137

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Effect of spraying techniques on the brown rot of peach fruit,and on black spot,powdery mildew and the two-spotted mite of apple trees     

James H. Whan  Ian R. Smith  Norman G. Morgan 《Pest management science》1983,14(6):609-614

Application by hand-held equipment of the commercially recommended doses of proprietary formulations of captan (9.0 g a. i. per tree), mancozeb (10.8 g a. i. per tree), oxythioquinox (1.1 g a. i. per tree), and cyhexatin (1.8 g a. i. per tree), in spray volumes ranging from 0.225 to 9.0 litres per tree, gave commercial control of the brown rot of peach fruit, and of black spot (apple scab), powdery mildew and the two-spotted mite (Tetranychus urticae) of apple trees. Commercial control was also obtained using a quarter of the recommended doses when applied in 0.225 or 2.25 litres per tree. The residue of oxythioquinox on the leaves, immediately after applying 1.1 g a. i. per tree, doubled as the volume of spray was reduced from 9.0 to 2.25 litres per tree, and increased further when the volume was reduced to 0.225 litres per tree. It is suggested that some of the oxythioquinox ran off the trees at the higher volumes, and that less run-off at lower volumes resulted in higher chemical residues.  相似文献   

THE SEROLOGICAL RESPONSE OF CHICKENS TO OIL EMULSION VACCINES CONTAINING THE V₄ STRAIN OF NEWCASTLE DISEASE VIRUS     

R. Schalkoort    D. M. Morgan  P. B. Spradbrow 《Australian veterinary journal》1982,58(6):260-263

SUMMARY Experiments were conducted with vaccines containing the V₄ strain of Newcastle disease virus (NDV). Both living aqueous vaccines and vaccines consisting of virus incorporated in an oil emulsion were used. The calculated dose of virus contained in the oil emulsion vaccine was 10^8,7 50% embryo infectious doses (EID₅₀) per bird dose. Haemagglutinin inhibition (HI) antibody levels of 8 are presumed protective. One-day-old chicks with low levels of maternal antibody were vaccinated intraocularly with 10^6,3EID₅₀ of live vaccine, and concurrently with oil emulsion vaccine. Presumed protective levels of antibody were present at two weeks post vaccination and were maintained for at least seven weeks longer. When adult birds 15 weeks old with no previous exposure to NDV were vaccinated intraocularly with 10^6,7EID₅₀ per bird, protective levels of antibody were produced within a week. Unvaccinated birds put in contact with the vaccinated birds produced similar antibody levels within 14 days. Revaccination with oil emulsion vaccine after antibody levels had fallen resulted in a rapid response with high levels of antibody. When antibody-free adult commercial birds with an unknown history of exposure to NDV were vaccinated intramuscularly with oil emulsion vaccine, high antibody levels were produced for at least 21 weeks. Concurrent intraocular inoculation with 10^7,0EID₅₀ live virus did not enhance the response. Natural infection of unvaccinated birds occurred during the experiment. This was detected by the presence of HI antibody levels of short duration. When antibody-free commercial birds were inoculated intramuscularly with oil emulsion vaccine containing 10^6,0, 10^7,0, or 10^8,0EID₅₀ per bird dose, 100% of birds inoculated with the highest dose produced presumed protective levels of antibody within two weeks, as compared with a 5-week delay when using the 10^7,0EID₅₀ per bird dose.  相似文献   

Investigation of aberrant positive reactions to serological tests for bovine brucellosis     

B A Rogerson  I R Morgan 《Australian veterinary journal》1986,63(7):227-229

Fifty cattle thought not to be infected with Brucella abortus but giving persistent positive serological reactions, were investigated. It was concluded that only one of these was infected since exhaustive bacteriological examination produced only one isolate of B. abortus (strain 19) and none of the herds of origin was subsequently shown to be infected with brucellosis. Antibody was detected in stifle joint fluid of 15 cattle, which may have been stimulated by the presence of Strain 19 antigen that persisted in collagenous tissue long after the viable organisms had been eliminated.  相似文献   

Vertebral canal and spinal cord mensuration: a comparative study of its effect on lumbosacral myelography in the dachshund and German shepherd dog   总被引：2，自引：0，他引：2  

J P Morgan  M Atilola  C S Bailey 《Journal of the American Veterinary Medical Association》1987,191(8):951-957

One hundred noncontrast spinal radiographic studies followed by myelography were evaluated to compare vertebral canal size and spinal cord location and size in a chondrodystrophic breed (50 Dachshunds) with those variables in a nonchondrodystrophic breed (50 German Shepherd Dogs). Measurements were made of the sagittal diameter (height) of the vertebral canal, sagittal diameter (height) of the spinal cord, and transverse diameter (width) of the spinal cord in the lumbar and sacral regions. Differences were detected in the craniocaudal location of maximal vertebral canal height and maximal spinal cord height. The spinal cords in the Dachshunds terminated further caudally than those in the German Shepherd Dogs. Location of maximal spinal cord width was different between the breeds, consistent with the apparent, more caudal termination of the cord in the Dachshunds. The ratio of spinal cord to vetebral canal heights was notably greater in the Dachshunds than in the German Shepherd Dogs. These differences in vertebral canal and spinal cord mensuration may influence the choice of radiographic technique and its interpretation. Injection sites may be selected further cranially in German Shepherd Dogs (L4-5) than in Dachshunds (L5-6).  相似文献