Evaluation of ELISA techniques for titration of monoclonal antibodies against botulinum toxin     

Y Kamata  S Kozaki  T Nagai  J Ogasawara  G Sakaguchi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1986,48(5):909-914

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Expression of CC chemokine receptor 4 (CCR4) mRNA in canine atopic skin lesion   总被引：1，自引：0，他引：1  

Maeda S  Okayama T  Omori K  Masuda K  Sakaguchi M  Ohno K  Tsujimoto H 《Veterinary immunology and immunopathology》2002,90(3-4):145-154

CC chemokine receptor 4 (CCR4) is a G protein-coupled seven transmembrane receptor that is selectively expressed on Th2 cells and plays an important role in the trafficking of Th2 cells into inflammatory sites. In this study, a full-length canine CCR4 cDNA was cloned and characterized in order to examine the potential role of CCR4 in allergic responses that produce skin lesions in canine atopic dermatitis (AD). The canine CCR4 cDNA reported in this study contained an open reading frame of 1083 nucleotides encoding 360 amino acids. The predicted amino acid sequence of canine CCR4 showed 91.9, 85.3 and 84.5% similarity with those of the human, mouse and guinea pig counterparts, respectively. Expression of CCR4 mRNA was detected in various tissues including thymus, spleen, heart, small intestine and lymph node. Furthermore, it was found that CCR4 mRNA was preferentially expressed in lesional skin of dogs with AD, together with the mRNA of thymus and activation-regulated chemokine (TARC), which is a ligand for CCR4. The present study demonstrates that CCR4 contributes strongly to the immunopathogenesis of canine AD.  相似文献   

Association between the cell-wall peptidoglycan and the progenitor toxin of Clostridium botulinum type C   总被引：1，自引：0，他引：1  

S H Hyun  G Sakaguchi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(1):169-176

Clostridium botulinum type C progenitor toxin with a molecular weight of 500 k daltons (C1 L toxin), purified from the bacterial cells, bound at pH 2 to the cell-wall peptidoglycan derived from certain strains. The carbohydrate moiety of the peptidoglycan contained arabinose and galactose at a certain ratio, both of which may directly be associated with the binding. The binding, being dependent on the quality and quantity of the sugars, enhanced the oral toxicity of the toxin to the chicken as well as the mouse.  相似文献   

¹H-NMR Method Enables Early Identification of Degeneration in the Quality of Sweet Potato Tubers     

M. Iwaya-Inoue  R. Matsui  N. Sultana  K. Saitou  K. Sakaguchi  M. Fukuyama 《Journal of Agronomy and Crop Science》2004,190(1):65-72

In sweet potato tuber, which is a tropical plant, long‐term storage leads to loss of water and carbohydrate, thus water mobility was investigated using ¹H‐NMR spectroscopy. Electrolyte leakage indicated that tubers stored at 15 °C for 1 year were partly injured and that frozen‐thawed tissues were dead. Nuclear magnetic resonance (NMR) spin–lattice relaxation time (T₁) and spin–spin relaxation time (T₂) clearly increased with the duration of storage, whereas these values decreased in the dead tissues. Furthermore, Arrhenius plots for T₁ and T₂ were determined at temperatures ranging from 20 to 0 °C in 2.5 °C steps. In the fresh tubers, a strong converse temperature dependency was shown in the T₂ measurement. On the contrary, there was no temperature dependency in the T₂ of the dead tissues. Thus, the existence of inverse temperature dependency reflected tissue viability. Additionally, any change in the T₂ of the fresh tubers occurred at about 14 °C, which virtually coincided with the storage temperature of 15 °C. The slope change in T₂ might have responded to a physiological change as a primary event. In conclusion, monitoring water status by NMR could provide early identification of changes in the quality of post‐harvest crops; this method shows great promise for use in environmental‐stressed crop yield research.  相似文献   

Immunoblot analysis for IgE-reactive components of fetal calf serum in dogs that developed allergic reactions after non-rabies vaccination     

Ohmori K  Masuda K  DeBoer DJ  Sakaguchi M  Tsujimoto H 《Veterinary immunology and immunopathology》2007,115(1-2):166-171

We previously demonstrated the presence of IgE directed to fetal calf serum (FCS) in the sera from dogs that developed allergic reactions after vaccination. In this study, by an immunoblot analysis, we investigated the IgE-reactive components of FCS using sera from 16 dogs that exhibited allergic reactions after vaccination. The immunoblot analysis revealed that several FCS proteins of approximately 25-, 50-, 66-, 75-, 120-, and 175-kDa strongly reacted with IgE in the sera from dogs that showed post-vaccination allergic reactions. The 66-kDa band was detected in the sera from 14 of the 16 dog serum samples analyzed in the immunoblot analysis for FCS, and it was speculated to be albumin based on its molecular weight; however, serum IgE reactivity to bovine serum albumin could be detected in only four of the 14 dog samples. These findings demonstrated that a variety of FCS components including albumin could function as allergens in dogs that developed allergic reactions after vaccination.  相似文献   

Metabolic profiles in ovulatory and anovulatory primiparous dairy cows during the first follicular wave postpartum     

Kawashima C  Sakaguchi M  Suzuki T  Sasamoto Y  Takahashi Y  Matsui M  Miyamoto A 《The Journal of reproduction and development》2007,53(1):113-120

Metabolic hormones affect ovarian function in the cow. However, the relationship between metabolic factors and ovarian function is not clear in the postpartum primiparous cow because they are still growing. The aim of the present study was to investigate in detail the time-dependent profile of the metabolic hormones, metabolites, and milk yields of ovulatory and anovulatory primiparous cows during the first follicular wave postpartum. We used 16 primiparous Holstein cows and obtained blood samples for the profiles of metabolites (glucose; non-esterified fatty acid, NEFA; ketone body; total cholesterol; and aspartate aminotransferase), metabolic hormones (growth hormone, GH; insulin-like growth factor-I, IGF-1; and insulin), and progesterone every other day from 1 to 21 days postpartum. In addition, all ovaries were observed using ultrasound. Dairy milk yield was recorded during the experimental period. In all cows, the first follicular wave postpartum was observed and 6 of the cows ovulated. The plasma glucose (P<0.0001) and IGF-1 (P<0.001) concentrations were lower and the plasma NEFA (P<0.0001) and ketone bodies (P<0.0001) concentrations and daily milk yield (P<0.0001) were higher in the anovulatory cows compared to the ovulatory cows. However, the GH levels, which enhance lipolysis for milk production, insulin and other metabolites did not differ between the two groups. In conclusion, the present study suggests that anovulation of the dominant follicle during the first follicular wave postpartum in primiparous cows is induced by low IGF-1 levels that are similar to those of multiparous cows. In addition, anovulatory cows are likely to mobilize body fat stores for milk production more easily than ovulatory cows.  相似文献   

IgE reactivity and cross-reactivity to Japanese cedar (Cryptomeria japonica) and cypress (Chamaecyparis obtusa) pollen allergens in dogs with atopic dermatitis     

Sakaguchi M  Masuda K  Yasueda H  Saito S  DeBoer DJ  Tsujimoto H 《Veterinary immunology and immunopathology》2001,83(1-2):69-77

The natural occurrence of Japanese cedar (Cryptomeria japonica) pollinosis has been reported in dogs with atopic dermatitis. However, the reactivity to Japanese cypress (Chamaecyparis obtusa) pollen allergens in these dogs has not been reported. The present study was designed to investigate the reactivity to Japanese cypress pollen allergens in dogs sensitized to Japanese cedar pollen allergens. In 19 dogs with specific IgE to C. japonica pollen allergen, we measured the specific IgE to C. obtusa pollen allergen and examined the reactivity to the allergen by intradermal test. Of the 19 dogs, 18 had specific IgE to crude and purified major allergens (Cha o 1) of C. obtusa pollen. Most of the dogs showed a positive reaction to C. obtusa pollen allergens in the intradermal test. Allergenic cross-reactivity between Cha o 1 and Cry j 1 (a major allergen in C. japonica pollen) was observed by the ELISA inhibition method. Dogs sensitized to Japanese cedar pollen allergens demonstrate reactivity to Japanese cypress pollen allergens.  相似文献   

Role of steriod hormones in sex change of protogynous wrasse   总被引：1，自引：0，他引：1  

Mikihiko Higa  Kei Ogasawara  Ayumi Sakaguchi  Yoshitaka Nagahama  Masaru Nakamura 《Fish physiology and biochemistry》2003,28(1-4):149-150

The endocrine mechanisms of sequential hermaphroditism were investigated in protogynous wrasse, Halichoeres trimaculatus. Administration of aromatase inhibitor and androgen both stimulated sex change, while estrogen suppressed the induced sex inversion. The results suggest that removal of estrogen is a key step to initiate sex change.  相似文献   

Distribution of immunoglobulin-positive cells in the spleen and kidney of Japanese flounder Paralichthys olivaceus   总被引：1，自引：0，他引：1  

Yuki Tokuda  Haruhiko Toyohara  Masaru Ikemoto  Tatsuo Kina  Morihiko Sakaguchi 《Fisheries Science》2000,66(6):1082-1086

SUMMARY: The distribution of surface immunoglobulin-positive (sIg⁺) cells in the spleen, the head-kidney, and the trunk-kidney of adult Japanese flounder Paralichthys olivaceus was investigated by a flow cytometric analysis using a monoclonal antibody FB17 against flounder immunoglobulin M. Although the percentage of sIg⁺ cells varied among organs, a good correlation was revealed between the percentage in the head-kidney and that in the trunk-kidney ( r = 0.996, P < 0.001). In contrast, no correlation was obtained between the percentage of sIg⁺ cells in the spleen and the head-kidney or trunk-kidney. The head-kidney and the trunk-kidney contained cells bearing fewer sIg that were stained dimly by FB17 in addition to brightly stained sIg⁺ cells regarded as mature B cells, while the spleen contained only sIg⁺ cells stained brightly. Moreover, the immunocytochemical analyses showed that the head-kidney contains plasma cells with strongly stained cytoplasm in addition to B cells with stained cell surface, which suggests that the cells with fewer sIg detected by flow cytometric analysis are presumed to be at a stage during the processes of differentiation to plasma cells. These results suggest that there are some differences between the spleen and kidney in the role of the immune system and it is considered that differentiation of B cells to plasma cells occurs in the kidney.  相似文献   

Development of a multiplex PCR and PCR-RFLP method for serotyping of Avibacterium paragallinarum   总被引：1，自引：0，他引：1  

Sakamoto R  Kino Y  Sakaguchi M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(2):271-273

Avibacterium (Haemophilus) paragallinarum (A. paragallinarum) is a causative agent of infectious coryza in chickens and is classified into three serovars by agglutination tests. In an effort to identify the serovars easily, PCR and PCR-RFLP were employed. As the target gene for PCR, the hypervariable region of HMTp210, which encodes the HA antigen, was used. PCR using primer sets around the hypervariable region amplified 0.8, 1.1 and 1.6 kbp fragments for serovars A, B and C, respectively. Alternatively, the 1.6 kbp fragments were amplified with another primer pair encompassing the hypervariable region and was subjected to digestion with Bgl II, which resulted in the detection of serovar-specific digestion patterns. These results indicate that PCR and PCR-RFLP using the hypervariable region of HMTp210 are alternative methods to identify the serovar of A. paragallinarum.  相似文献