Occurrence, pathogenicity and distribution of Fusarium spp. in stored wheat seeds Kermanshah Province, Iran     

Chehri K  Salleh B  Yli-Mattila T  Soleimani MJ  Yousefi AR 《Pakistan journal of biological sciences: PJBS》2010,13(24):1178-1186

Fusarium is one of the most important pathogenic and toxigenic fungi widely distributed all over the world, including Iran. Fusarium species are found frequently in stored agriculture products especially wheat. The objective of this study was to identify Fusarium species associated with stored wheat seeds and their pathogenicity on root and head of wheat in Kermanshah, the leading province in wheat production in Iran. In this survey 75 seed samples of stored wheat were collected from 10 different regions during 2006-2008 and tested for the presence of Fusarium. Fusarium spp. were found in 51 (68%) of 75 samples. A total of 580 Fusarium strains were isolated, identified and preserved. All these strains belong to 20 Fusarium spp. according to morphological characters. Each conidial suspension of selected strains representing all species was evaluated for their pathogenicity on roots and spikes of healthy wheat var. Fallat in the greenhouse. F. graminearum, F. crookwellense, F. trichothecioides, F. culmorum and F. verticillioides were the most pathogenic to wheat's head. Foot rot assessment revealed that F. pseudograminearum and F. culmorum were the most damaging species. Of the Fusarium isolates, F. graminearum was the most prevalent followed by F. verticillioides and F. proliferatum. This is the first comprehensive report on identity and distribution of Fusarium spp. from stored wheat seeds in Iran while F. nelsonii was reported for the first time from wheat seeds in Iran.  相似文献   

Functionalization of graphene nanosheets and its dispersion in PMMA/PEO blend: Thermal,electrical, morphological and rheological analyses     

Elham Aram  Morteza Ehsani  Hossein Ali Khonakdar  Seyed Hassan Jafari  Nastaran Riahi Nouri 《Fibers and Polymers》2016,17(2):174-180

Graphene nanoplatelet (GnP) was chemically functionalized by amine groups for improvement of compatibility in poly(methyl methacrylate) (PMMA)/poly(ethylene oxide) (PEO) blend. PMMA/PEO (90/10) nanocomposites with non-functionalized GnP and functionalized GnP (FGnP) were prepared by solution casting method. Successful grafting of amine groups on the GnP surface was confirmed by Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) analysis. The Transmission electron microscopy (TEM) images showed that the dispersion state of FGnP was better than that of GnP in PMMA/PEO nanocomposites. The effects of FGnP and GnP on rheological, thermal and electrical properties of PMMA/PEO nanocomposites were investigated by various methods. The results indicated that the FGnP-based nanocomposites had higher storage modulus, glass transition temperature and thermal stability as compared to the GnP-based nanocomposites. The electrical conductivity of the nanocomposites with FGnP was better than that of GnP-based nanocomposites. The higher conductivity was attributed to homogeneous and well dispersion state of FGnP in PMMA/PEO nanocomposites.  相似文献   

No detected hepatitis B virus-DNA in thalassemic patients infected by hepatitis C virus in Kerman province of Iran     

Arababadi MK  Hassanshahi G  Yousefi H  Zarandi ER  Moradi M  Mahmoodi M 《Pakistan journal of biological sciences: PJBS》2008,11(13):1738-1741

This research was aimed to investigate the prevalence and clinical impact of occult HBV infection in thalassemic patients with chronic HCV infection. In this cross-sectional study we have totally examined 60 patients suffering HBV and HCV infections by PCR and RT-PCR methods, respectively, in Kerman province of Iran. ELISA technique (RADIM, Italy) was used to detect anti-HBc, anti-HBs and HBsAg. The serum level of liver enzymes (SGOT, SGPT, DB, TB and ALK) were analyzed in the HCV infected patients (MAN, IRAN). Statistical analyses performed using t-test and Chi-square. We found that 27 cases (out of 60) were infected by HCV but HBV-DNA was not seen in HCV infected patients. Present findings also showed that none of samples were HBsAg positive but 9 (33%) (out of 27) HCV-RNA positive patients were anti-HBc positive and 11 (40.7%) were positive for anti-HBs. We found that SGOT, SGPT, DB, TB and ALK are above normal in 27 (100%), 19(70.3%), 12(44.5%), 15 (55.5%) and 15 (55.5%) RNA-HCV positive patients, respectively. The prevalence of hepatitis C infection is very high in thalassemic patients and based on other studies our results showed that the prevalence of HCV infection in Kerman is more than other provinces of Iran. In contrast with other studies HBV-DNA in these patients could not be detected, hence, it seems that occult HBV infection isn't frequent in Iranian thalassemic patients who suffering from chronic HCV infection.  相似文献   

Effects of linalool on physiological responses of Cyprinus carpio (Linnaeus, 1758) and water physico‐chemical parameters during transportation          下载免费PDF全文

Mohammad Mazandarani  Seyyed Morteza Hoseini  Meisam Dehghani Ghomshani 《Aquaculture Research》2017,48(12):5775-5781

This study investigated the potential of linalool as an anaesthetic during transportation of common carp, Cyprinus carpio. The fish were transported at a loading density of ~103 g/L for 3 hr in 12 plastic bags (3 L water and 6 L pure oxygen) divided into four triplicated treatments: control (without linalool), L50 (50 mg linalool/L), L100 (100 mg linalool/L) and L200 (200 mg linalool/L). After 3‐hr transportation, serum physiological responses and water physico‐chemical parameters were compared among the treatments. Results showed that water total and un‐ionized ammonia increased and dissolved oxygen decreased in all treatments after transportation. Water total ammonia and dissolved oxygen levels in the linalool‐treated bags were significantly lower than the control. After transportation, the control fish showed significant elevation in serum cortisol and glucose levels; however, the levels were significantly lower than the linalool‐treated fish. All fish showed similar serum lactate levels, significantly lower than the value obtained before transportation. The control fish showed serum urea levels similar to the level obtained before transportation; however, the linalool‐treated fish had significantly higher serum urea levels compared to the control and before transportation levels. After transportation, all fish had decreased serum chloride and sodium levels compared to the before transport level. Overall, the present results showed that linalool is not beneficial for carp transportation in plastic bags, because it reacts with water oxygen, increases stress in fish, interferes with ammonia excretion and has no benefits in preventing ion loss.  相似文献   

Effects of dietary essential amino acid deficiencies on the growth performance and humoral immune response in silvery‐black porgy (Sparidentex hasta) juveniles          下载免费PDF全文

Morteza Yaghoubi  Mansour Torfi Mozanzadeh  Jasem G. Marammazi  Omid Safari  Enric Gisbert 《Aquaculture Research》2017,48(10):5311-5323

A 6‐week feeding trial was conducted for determining the effects of dietary essential amino acids (EAA) deficiencies on growth performance and non‐specific immune responses in silvery‐black porgy juveniles (4.7 ± 0.1 g initial weight). Eleven isoproteic (ca. 47%) diets were formulated including a control diet containing the optimum quantity of EAA, and ten EAA‐deficient diets. All diets contained 36% fish meal and 18.5% crystalline EAA and non‐essential amino acids (NEAA) as the main source of dietary proteins. All the EAA and NEAA incorporated in the crystalline amino acids mixture of the control diet simulated the amino acids profile of the fish meal. The other 10 EAA‐deficient diets were formulated by the deletion of each of the 10 EAA (crystalline form) from the control diet and replaced by a mixture of NEAA for the adjustment of dietary nitrogen contents. At the end of the experiment, fish fed with threonine‐deficient diet showed the lowest survival rate (p < .05), whereas growth performance decreased in fish fed all EAA‐deficient diets, although the reduction in body growth varied depending on the EAA considered. Plasma total protein decreased in all experimental groups except for fish fed the phenylalanine‐deficient diet. Fish fed with arginine‐ and lysine‐deficient diets had the lowest plasma C3, C4, lysozyme, total immunoglobulin and total superoxide dismutase activity (p < .05). Present results indicated that lysine, methionine and threonine were the most limiting EAA in terms of growth performance; however, arginine, threonine and lysine were the most limiting EAA for innate immunity responses in silvery‐black porgy juveniles.  相似文献   

Dietary 1,8‐cinoele affects serum enzymatic activities and immunological characteristics in common carp (Cyprinus carpio) exposed to ambient ammonia     

Ali Taheri Mirghaed  Sahel Fayaz  Seyyed Morteza Hoseini 《Aquaculture Research》2019,50(1):146-153

The present study aimed to investigate effects of dietary 1,8‐cineole (cineole) on common carp (Cyprinus carpio) health under ammonia toxicity. The fish were fed with four diets supplemented by 0 (control), 0.1 (0.1C), 0.5 (0.5C), and 1 (1C) percent cineole for 2 weeks, before a 24‐hr ammonia exposure. Blood samples were taken before and after ammonia exposure. The results showed that ammonia exposure significantly (p < 0.05) increased serum urea levels, and decreased serum alternative complement (ACH50) activity and serum total immunoglobulin (Ig) levels. Cineole significantly (p < 0.05) increased serum ACH50 activity (0.1% and 0.5% levels) and total Ig levels (0.5% levels). There was no significant difference (p > 0.05) in serum total protein, albumin and globulin levels among the treatments. Before ammonia exposure, there was no significant difference (p > 0.05) in serum alkaline phosphatase (ALP), alanine transaminase (ALT), and lysozyme activities among the cineole treatments. The treatment 0.5C had the lowest lactate dehydrogenase (LDH) and aspartate transaminase (AST) activities before ammonia exposure. After ammonia exposure, serum LDH and ALT activities increased in all treatments, but the treatment 0.5C had the lowest activities. Serum ALP activities increased after the ammonia exposure in the control and 1C groups. Serum AST and lysozyme activities increased after the challenge in all groups except for the 0.5C group. Overall, the results suggest that ammonia exposure causes increase in serum LDH, ALP, AST, and ALT activities and decreased immune responses, which are indicator of tissue damage and immunosuppression. 0.5% cineole is capable to suppress adverse effects of ammonia intoxication in carp.  相似文献   

Combined or individual effects of dietary magnesium and selenium nanoparticles on growth performance,immunity, blood biochemistry and antioxidant status of Asian seabass (Lates calcarifer) reared in freshwater     

Morteza Longbaf Dezfouli  Amin Ghaedtaheri  Saeed Keyvanshokooh  Amir Parviz Salati  Seyed Mohammad Mousavi  Hossein Pasha‐Zanoosi 《Aquaculture Nutrition》2019,25(6):1422-1430

This study was conducted to investigate effects of and interactions between nanoselenium (NanoSe) and nanomagnesium (NanoMg) on growth, humoral immunity, serum biochemistry and antioxidant capacity of juvenile Asian seabass Lates calcarifer reared in freshwater. Four groups of fish with an average weight of 32.78 ± 2.23 g were fed one of the experimental diets for 6 weeks: (a) control (basal diet); (b) NanoSe (basal diet + 4 mg NanoSe/kg diet); (c) NanoMg (basal diet + 500 mg NanoMg/kg diet); and (d) combination (basal diet + 4 mg NanoSe/kg diet + 500 mg NanoMg/kg diet). Fish fed with NanoSe‐supplemented diets (NanoSe and combination) showed higher weight gain, specific growth rate and feed intake. The combination of NanoSe and NanoMg enhanced the immune response. Also, fish fed on combination diet showed higher serum alanine aminotransferase and aspartate transaminase levels but lower glucose concentration. The activities of liver superoxide dismutase, glutathione peroxidase and catalase were not different among all groups, but liver malondialdehyde level was lower in fish fed diets supplemented with NanoSe and/or NanoMg. It could be concluded that NanoSe improved growth performance; the combination of both NanoSe and NanoMg enhanced humoral immunity; and NanoSe and/or NanoMg improved antioxidant capacity in Asian seabass.  相似文献   

Enhanced haematological and immune responses in common carp Cyprinus carpio fed with olive leaf extract‐supplemented diets and subjected to ambient ammonia     

Hamid Rajabiesterabadi  Morteza Yousefi  Seyyed Morteza Hoseini 《Aquaculture Nutrition》2020,26(3):763-771

The aim of the present study was to evaluate the potential effects of olive leaf (Olea europea L.) extract (OLE) on common carp (Cyprinus carpio) health in response to ambient ammonia toxicity. Fish were fed with experimental diets containing OLE (0, 1, 5 and 10 g/kg) for 60 days followed by a 3‐hr challenge with ammonia (0.5 mg/L unionized ammonia‐N). Results showed that haemoglobin levels, differential leucocyte counts and mean corpuscular haemoglobin concentration were not affected by dietary OLE levels and ammonia challenge. Ammonia challenge significantly increased red blood cells and decreased blood mean corpuscular volume, mean corpuscular haemoglobin, plasma lysozyme, complement and bactericidal activities. Plasma lysozyme, complement and total immunoglobulin values improved by 5 and/or 10 g OLE/kg supplementation. Before the ammonia exposure, the 5 and 10 g OLE/kg treatment significantly increased blood white blood cell, but there was no significant difference among the treatments after the ammonia exposure. Ammonia exposure significantly increased plasma malondialdehyde levels; however, 1 and 5 g OLE/kg treatments had significantly lower values compared to the other treatments. The present results suggest that 1 g OLE/kg diet may improve fish health and reduce the adverse effects of ammonia toxicity on common carp.  相似文献   

Effect of dietary l-tryptophan on osmotic stress tolerance in common carp, Cyprinus carpio, juveniles     

Seyyed Morteza Hoseini  Seyed Abbas Hosseini 《Fish physiology and biochemistry》2010,36(4):1061-1067

Common carp juveniles were fed two types of diet (control: 0.1% tryptophan and TRP: 0.6% tryptophan) over 15 days. Thereafter, both groups were directly subjected to osmotic challenge (from 0 to 10 ppt) for 168 h. Blood samples were collected at ?240, 0, 6, 24, 72 and 168 h after challenge. Survival and serum cortisol, glucose, sodium and chloride levels were measured to determine stress response and osmoregulation condition. While TRP group showed no mortality until 168 h, cumulative mortality was near 100% at 72 h after challenge in control group. Feeding tryptophan-supplemented diet led to increase in prechallenge cortisol, but not glucose, sodium and chloride values at ?240 h that stayed elevated until 0 h after challenge. After challenge, cortisol and glucose values did not significantly affected by time of sampling but type of diet and diet × time interaction. Sodium values significantly affected by diet type and time of sampling but not their interaction. Chloride values significantly affected by time of sampling but not diet type and their interaction. Control group had higher cortisol, glucose and sodium than TRP at each time of sampling. Control and TRP group showed increasing and decreasing pattern in cortisol and glucose values when experiment progressed after osmotic challenge. Both group showed increasing pattern in sodium and chloride values when experiment progressed after osmotic challenge. Results indicated that tryptophan supplementation enhanced salt water tolerance of carp that is due to increase in basal cortisol and anti-stress effect of tryptophan and possibly increase in serotonergic activity.  相似文献   

Total phenol,anthocyanin, and terpenoid content,photosynthetic rate,and nutrient uptake of Solanum nigrum L. and Digitaria sanguinalis L. as affected by arbuscular mycorrhizal fungi inoculation     

Sakineh Rashidi  Ali Reza Yousefi  Majid Pouryousef  Nieves Goicoechea 《Weed Biology and Management》2020,20(3):95-108

Over the last decades, tillage, chemical fertilizers, and pesticides have reduced the beneficial fungal population size in arable soils. Though soil inoculation can be a practical way to restore arbuscular mycorrhizal fungi (AMF) population size, weeds may also be benefited, as well. This study was aimed to evaluate the effect of three AMF species (Funneliformis mosseae, Rhizoglomus fasciculatum, and Rhizoglomus intraradices) on photosynthetic rate, secondary metabolites content, reproductive organs percentage and nutrient uptake in Solanum nigrum L. and Digitaria sanguinalis L. weed species. Our results showed species variation in response to AMF inoculation, so that, while inoculation with R. intraradices fungal species decreased total biomass in S. nigrum plants significantly, it increased total biomass of D. sanguinalis plants by 26–49%. In addition, inoculation with F. mosseae species increased phenol, anthocyanin, and total terpenoid content in S. nigrum plants much more than D. sanguinalis. Increased photosynthetic rate, secondary metabolites content, and flowering percentage in AMF‐inoculated S. nigrum plants show the enhanced competitive ability and allelopathic potential of this weed when associated with AMF, which makes it a good competitor against other plant species in the environment.  相似文献