全文获取类型
收费全文 | 2067篇 |
免费 | 141篇 |
国内免费 | 148篇 |
专业分类
林业 | 245篇 |
农学 | 238篇 |
基础科学 | 101篇 |
322篇 | |
综合类 | 575篇 |
农作物 | 72篇 |
水产渔业 | 106篇 |
畜牧兽医 | 378篇 |
园艺 | 136篇 |
植物保护 | 183篇 |
出版年
2024年 | 5篇 |
2023年 | 22篇 |
2022年 | 43篇 |
2021年 | 58篇 |
2020年 | 81篇 |
2019年 | 56篇 |
2018年 | 57篇 |
2017年 | 78篇 |
2016年 | 75篇 |
2015年 | 87篇 |
2014年 | 94篇 |
2013年 | 120篇 |
2012年 | 148篇 |
2011年 | 167篇 |
2010年 | 108篇 |
2009年 | 111篇 |
2008年 | 124篇 |
2007年 | 137篇 |
2006年 | 91篇 |
2005年 | 94篇 |
2004年 | 42篇 |
2003年 | 47篇 |
2002年 | 57篇 |
2001年 | 66篇 |
2000年 | 56篇 |
1999年 | 74篇 |
1998年 | 37篇 |
1997年 | 28篇 |
1996年 | 29篇 |
1995年 | 26篇 |
1994年 | 29篇 |
1993年 | 22篇 |
1992年 | 22篇 |
1991年 | 9篇 |
1990年 | 12篇 |
1989年 | 10篇 |
1988年 | 10篇 |
1987年 | 8篇 |
1986年 | 4篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1974年 | 1篇 |
1967年 | 1篇 |
1965年 | 1篇 |
1943年 | 1篇 |
1940年 | 1篇 |
排序方式: 共有2356条查询结果,搜索用时 15 毫秒
81.
诊断敏感性和特异性是诊断方法验证过程中的两个重要参数。本文通过分析和比较,详细阐述了诊断敏感性和特异性在动物检疫工作中的重要性,厘清了其与分析敏感性和特异性的区别。通过计算分析比较了不同流行率对检测结果可信度的影响,以及在同一流行率下,诊断敏感性和特异性变化对检测结果可信度的影响。结果显示,当流行率低时,诊断特异性对结果可信度的影响更大,诊断特异性的降低会导致阳性检测结果可信度显著下降。因此在进出境动物检疫工作中,不要一味强调分析特性,而要重视诊断特性;既要关注诊断敏感性,做到不漏检;也要关注诊断特异性,做到不误检。 相似文献
82.
Effect of Chronic Administration of Phenobarbital,or Bromide,on Pharmacokinetics of Levetiracetam in Dogs with Epilepsy 下载免费PDF全文
K.R. Muñana J.A. Nettifee‐Osborne M.G. Papich 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(2):614-619
Background
Levetiracetam (LEV) is a common add‐on antiepileptic drug (AED) in dogs with refractory seizures. Concurrent phenobarbital administration alters the disposition of LEV in healthy dogs.Hypothesis/Objectives
To evaluate the pharmacokinetics of LEV in dogs with epilepsy when administered concurrently with conventional AEDs.Animals
Eighteen client‐owned dogs on maintenance treatment with LEV and phenobarbital (PB group, n = 6), LEV and bromide (BR group, n = 6) or LEV, phenobarbital and bromide (PB–BR group, n = 6).Methods
Prospective pharmacokinetic study. Blood samples were collected at 0, 1, 2, 4, and 6 hours after LEV administration. Plasma LEV concentrations were determined by high‐pressure liquid chromatography. To account for dose differences among dogs, LEV concentrations were normalized to the mean study dose (26.4 mg/kg). Pharmacokinetic analysis was performed on adjusted concentrations, using a noncompartmental method, and area‐under‐the‐curve (AUC) calculated to the last measured time point.Results
Compared to the PB and PB–BR groups, the BR group had significantly higher peak concentration (C max) (73.4 ± 24.0 versus 37.5 ± 13.7 and 26.5 ± 8.96 μg/mL, respectively, P < .001) and AUC (329 ± 114 versus 140 ± 64.7 and 98.7 ± 42.2 h*μg/mL, respectively, P < .001), and significantly lower clearance (CL/F) (71.8 ± 22.1 versus 187 ± 81.9 and 269 ± 127 mL/h/kg, respectively, P = .028).Conclusions and Clinical Importance
Concurrent administration of PB alone or in combination with bromide increases LEV clearance in epileptic dogs compared to concurrent administration of bromide alone. Dosage increases might be indicated when utilizing LEV as add‐on treatment with phenobarbital in dogs. 相似文献83.
84.
85.
86.
Silvana Nisgoski Mayara E Carneiro Elaine C Lengowski Felipe Z Schardosin Graciela I Bolzon de Muñiz 《Southern Forests》2015,77(4):243-247
The correct identification of pine species is necessary for proper application of wood in forest?based industries, since the quality of each species’ wood depends on factors intrinsic to the material. The aim of this study was to evaluate the potential use of near?infrared and visible spectroscopy in the discrimination of pine species planted in southern Brazil. Needles of Pinus clausa, P. glabra, P. kesiya, P. oocarpa, P. palustris, P. pseudostrobus, P. rigida, P. roxburghii and P. serotina were collected from experimental plantations located in the region of Rio Negro, Paraná, Brazil. The needles were dried and milled for analysis. The evaluation was performed with a spectral range of 400–750?nm (visible) and 1 000–2 500?nm (near?infrared). Analysis using the visible spectra resulted in two principal components explaining 95% of the variation between the needle samples. In the near?infrared analysis, it was possible to discriminate between all nine pine species studied using only two principal components, where the first explained 99% of the variation between species. Spectroscopy based on needles can be used for pine species discrimination, using the original data without mathematical treatment, in southern Brazil. 相似文献
87.
Muñoz J Garcia-Molina F Varon R Rodriguez-Lopez JN García-Ruiz PA García-Canovas F Tudela J 《Journal of agricultural and food chemistry》2007,55(3):920-928
Chlorogenic acid is the major diphenol of many fruits, where it is oxidized enzymatically by polyphenol oxidase (PPO) or peroxidase (POD) to its o-quinone. In spectrophotometric studies of chlorogenic acid oxidation with a periodate ratio of [CGA]0/[IO4-]0 < 1 and [CGA]0/[IO4-]0 > 1, the o-quinone was characterized as follows: lambda(max) at 400 nm and epsilon = 2000 and 2200 M-1 cm-1 at pH 4.5 and 7.0, respectively. In studies of o-quinone generated by the oxidation of chlorogenic acid using a periodate at ratio of [CGA]0/[IO4-]0 > 1, a reaction with the remaining substrate was detected, showing rate constants of k = 2.73 +/- 0.17 M-1 s-1 and k' = 0.05 +/- 0.01 M-1 s-1 at the above pH values. A chronometric spectrophotometric method is proposed to kinetically characterize the action of the PPO or POD on the basis of measuring the time it takes for a given amount of ascorbic acid to be consumed in the reaction with the o-quinone. The kinetic constants of mushroom PPO and horseradish POD are determined. 相似文献
88.
Antioxidant profile of red wines evaluated by total antioxidant capacity, scavenger activity, and biomarkers of oxidative stress methodologies 总被引:4,自引:0,他引:4
Rivero-Pérez MD Muñiz P Gonzalez-Sanjosé ML 《Journal of agricultural and food chemistry》2007,55(14):5476-5483
The study of the antioxidant capacity of foodstuffs requires the use of diverse determination methods to gain a wider picture of their multiple effects. The aim of this work was to evaluate the "antioxidant profile" of red wines applying TAC (total antioxidant capacity) methods: 2,2'-azinobis(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl, N,N-dimethyl-p-phenylenediamine dihydrochloride, oxygen radical absorbance capacity, ferric reducing/antioxidant power, hydroxyl and superoxide radical scavenger activities, and biomarkers of oxidative stress methods such as lipid peroxidation inhibition and inhibition of damage to DNA. Furthermore, levels of total polyphenols (TPP) of wines were also evaluated. Three bottles of 107 different Spanish red wines (total samples 321), made from different grape varieties, aging processes, and vintages, were analyzed. The validation of TAC methods, the first step in this work, provided a good linearity, proportionality, and low detection limits. Among these methods, the ABTS was the most satisfactory for its rapidity, cost, and precision. All wines showed an important capacity to scavenge hydroxyl radicals and were capable of blocking superoxide radicals but with 10 times lower intensity. Wines also showed important protective action on biomarkers of oxidative stress; they were much more active to inhibit lipid peroxidation than DNA oxidation. Few statistically significant correlations among levels of TPP and antioxidant properties of wines were detected. Furthermore, values of these correlations were very low. 相似文献
89.
Fernandez-Orozco R Frias J Muñoz R Zielinski H Piskula MK Kozlowska H Vidal-Valverde C 《Journal of agricultural and food chemistry》2007,55(22):8972-8979
The effect of fermentation on the antioxidant compounds [vitamins C and E, total phenolic compounds (TPC), and reduced glutathione (GSH)], and antioxidant capacity [superoxide anion scavenging activity (SOD-like activity), peroxyl radical-trapping capacity (PRTC), inhibition of phosphatidylcholine (PC) peroxidation, and Trolox equivalent antioxidant capacity (TEAC)] of soybean (Glycine max cv. Merit) was studied. Fermentation was carried out in solid state in cracked seeds inoculated with Aspergillus oryzae, Rhizopus oryzae, Bacillus subtilis, and Lactobacillus plantarum and in liquid state either in cracked seeds or milled soybean flours fermented naturally by only the microorganisms present in the seeds or by inoculation with L. plantarum. Vitamin C was not detected in the studied samples. Fermentation caused a decrease in vitamin E activity, except when cracked seed was fermented with A. oryzae, R. oryzae, or B. subtilis that increased 31, 30, and 89%, respectively. Fermentation produced an increase in TPC content and did not affect or reduce the GSH content. Fermentation decreased SOD-like activity drastically, while PRTC increased except when it was carried out naturally in cracked seed. TEAC values rose sharply when soybeans were fermented with B. subtilis. Processed soybean extracts inhibited PC peroxidation in comparison with the control assay. On the basis of the results obtained, the relative contributions of vitamin E, TPC, and GSH to antioxidant capacity were calculated and results showed a very high TPC contribution and a low contribution of GSH and vitamin E activity. Optimum results for functional soybean flours were achieved when fermentation was carried out with B. subtilis inoculum. 相似文献
90.
为科学评价和充分利用花生新品种,本研究运用隶属函数法对12个花生品种品质的5项主要指标进行了综合分析。结果表明,隶属函数值不低于对照‘花育33号’的品种有3个,分别是‘中花224’、‘云花1号’、‘花育9122’,这3个品种综合品质较优,适宜在北方地区种植推广。其中,‘中花224’油酸含量大于75%且O/L>10,可作为优质高油酸花生种质资源利用;‘云花1号’粗蛋白含量相对较高,具有较好的营养品质,可作为优质鲜食花生品种;‘花育9122’含油量较高,可作为油用加工型花生品种。 相似文献