Development of a semi-selective medium and an immunofluorescence colony-staining procedure for the detection of Clavibacter michiganensis subsp. sepedonicus in cattle manure slurry     

N. J. M. Roozen  J. W. L. Van Vuurde 《European journal of plant pathology / European Foundation for Plant Pathology》1991,97(5):321-334

Various compounds and basal media were tested for their suitability to create a semi-selective medium for isolation ofClavibacter michiganensis subsp.sepedonicus (Cms) from cattle manure slurry containing c. 10⁸ colony forming units (cfu) per ml.Plating efficiency of Cms in yeast glucose mineral medium (YGM) was 104% compared with yeast peptone glucose medium. Nalidixic acid, polymyxin B sulphate and the experimental disinfectant S-0208 inhibited colony growth of cattle slurry bacteria as compared with Cms in YGM. The optimal concentration of these inhibitors in combination was determined by modified agar diffusion tests and by pour plating in 24-well tissue culture plates. The semi-selective medium YGMI consisted of YGM supplemented with nalidixic acid (2 mg/l), polymyxin B sulphate (30 mg/l) and S-0208 (125 mg/l). Plating efficiency varied for Cms between 50.9 and 69.6%, for cattle slurry bacteria between 1.8 and 2.5% and for saprophytes from potato heel end extracts between 11.5 and 27.4%.Differentiation of Cms colonies from other colonies was based on their small and bluish colony morphology in pour plates and on immunofluorescence colony-staining (IFC). IFC of a pure culture of micro colonies of Cms in YGM was possible after one day incubation (colonies c. 5 cells). Green background fluorescence in the agar gels was prevented by addition of Tween 20 (0.1%) to the washing buffer and the use of 1% agar gels. IFC of macro colonies of Cms in YGMI, visible with 4x objective magnification, was possible after 4 days. The detection level of the target organism in artificially inoculated cattle slurry in YGMI based on colony morphology varied between 1.4×10³ and 2.3×10⁴ cfu per ml of cattle slurry. Miniaturized plating combined with IFC, using wells in tissue culture plates (=16 mm), proved suitable for detection, but was c. 30 times les sensitive. The recovery of Cms was negatively correlated with the number of saprophytic colonies in the agar plates (R ²=0.74).  相似文献   

Parallel supercomputers for lattice gauge theory     

Brown FR  Christ NH 《Science (New York, N.Y.)》1988,239(4846):1393-1400

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Sedimentary studies: desert sediments     

Lancaster N 《Science (New York, N.Y.)》1988,241(4862):233-234

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Murderous Science. Elimination by Scientific Selection of Jews, Gypsies, and Others, Germany, 1933-1945. Benno Muller-Hill. Oxford University Press, New York, 1988. xvi, 208 pp. $24.95. Translated from the German edition (Reinbek bei Hamburg, 1984) by George R. Fraser     

Proctor RN 《Science (New York, N.Y.)》1988,241(4866):730-731

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Dynamic properties of molecularly thin liquid films     

Israelachvili JN  McGuiggan PM  Homola AM 《Science (New York, N.Y.)》1988,240(4849):189-191

An experimental technique is described for simultaneously measuring the static and dynamic interactions of very thin liquid films between two surfaces as they are moved normally or laterally relative to each other. Film thickness can be measured and controlled to 1 angstrom. Initial results are presented of the transition in the physical properties of liquid films only one molecular layer thick to thicker films whose properties are practically indistinguishable from the bulk. In particular, the results show that two molecularly smooth surfaces, when close together in simple liquids, slide (shear) past each other while separated by a discrete number of molecular layers, and that the frictional force is "quantized" with the number of layers.  相似文献   

Infertility in a ram associated with a knobbed acrosome abnormality of the spermatozoa   总被引：1，自引：1，他引：0       下载免费PDF全文

Savage NC 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(3):126-127

A yearling Rambouillet ram with an asymmetrical scrotum was examined for potential breeding soundness prior to use in a synchronized mating program in a purebred flock of 20 ewes. Initial sperm cell evaluation revealed 78% knobbed acrosomes associated with few other abnormalities of the head and midpiece. Use of the ram resulted in no conception in one group of ten synchronized ewes. One month later, the proportion of sperm cells with knobbed acrosomes was 80%.  相似文献   

Les différentes bandes de contention utilisées pour la confection de prothèses externes chez les petits animaux          下载免费PDF全文

Bonneau NH  Olivieri M 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(5):199-200

Retrospective evaluation of xylazine/halothane anesthesia in 125 cattle          下载免费PDF全文

Adetunji A  Pascoe PJ  McDonell WN  Horney FD 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(9):342-346

A retrospective morbidity and mortality study was carried out on 125 case records of cattle involving xylazine/halothane anesthesia at the Ontario Veterinary College, Guelph between May 1979 and February 1983.  相似文献   

Un ostéosarcome de la diaphyse du fémur d'un chien, associé à un implant métallique          下载免费PDF全文

Breton L  Bonneau NH  Beauregard M 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(11):426-428

The hymenopterous parasites of Nitidulidae     

Roger N. Williams  Michael J. Weiss  M. Kehat  D. Blumberg 《Phytoparasitica》1984,12(1):53-64

The known information on hymenopterous parasites of Nitidulidae was consolidated in this article. Species of parasites, nitidulid hosts and, where possible, plant host of the nitidulids are provided along with citations to sources of information. Thirty-three species of hymenopterous parasites are recorded in nine different families. Since nomenclature of several of the parasites has changed, it was updated with the assistance of specialists.  相似文献