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排序方式： 共有228条查询结果，搜索用时 140 毫秒
81.
Claudineia Lizieri Kacilda Naomi Kuki Rosane Aguiar 《Water, air, and soil pollution》2012,223(5):2807-2820
Among the many anthropogenic abiotic stresses, manganese (Mn) toxicity has been recognized for its impact on aquatic ecosystems as well as on the biological components of these ecosystems, including aquatic plants. The objective of this study was to determine the Mn accumulation ability of aquatic macrophytes (Azolla caroliniana, Salvinia minima and Spirodela polyrhiza) and evaluate the morphophysiological responses of the species that gather the highest amount of Mn when exposed to a supra-optimal supply of manganese. The experiments were conducted in the laboratory, and the effects of Mn were evaluated based on plant growth; the concentration of total chlorophyll, carotenoids, and anthocyanins; the enzymatic activity of catalase and peroxidase; and leaf anatomy. All of the studied species accumulated Mn in their tissues. Moreover, it was observed that this accumulation was dependent on the concentration of the metal in solution. S. polyrhiza showed higher concentrations of Mn in its tissues (17.062?mg?g?1 dry weight (DW)), followed by S. minima (4.283?mg?g?1 DW) and A. caroliniana (1.341?mg?g?1 DW). Despite the Mn accumulation in all species, S. polyrhiza was the only one selected for further analyses because of its greater ability to accumulate Mn. The high Mn concentration found in tissues of S. polyrhiza suggests that this species has the potential to sequester and accumulate this metal. However, a sensitive response in the plants exposed to higher Mn concentrations (0.4?mM) was observed. The phytotoxicity effects of this accumulation were responsible for a decrease in the plant growth, a reduction in the pigment content (total chlorophyll, carotenoids, and anthocyanins), a low activity of catalase, and the disarrangement of the leaf aerenchyma. 相似文献
82.
Most durum wheat (Triticum durum) varieties possess only low winter hardiness due to their frost susceptibility. In North America and Central Europe, durum wheat is therefore typically sown in spring to circumvent the local winter conditions. However, the yield potential of durum in these regions could be much better exploited if durum varieties with increased frost tolerance were available, which could be sown in autumn. A factor limiting breeding for increased frost tolerance is the variation in the occurrence of frost stress across years. The ‘Weihenstephaner Auswinterungsanlage’ is a semi‐controlled test that exposes the plants to all weather conditions. Snow coverage of the plants, serving as frost protection, is prevented by the movable glass lid of the semi‐controlled test. In this study, different scorings for frost tolerance based on this semi‐controlled test were evaluated and compared with frost tolerance data in the field. Our results illustrate the potential of the ‘Weihenstephaner Auswinterungsanlage’ as an indirect selection tool for frost tolerance in durum breeding programmes, especially when regular frost tolerance data from the field are not available. 相似文献
83.
Shinichi WATANABE Megumi MIURA Hiromi MORITA Moeka NISHI Shin-ichi YOKOTA Shosaku HATTORI Hiromichi MATSUMOTO Emiko FUKUI Ken Takeshi KUSAKABE Masanori OCHI Naomi NAKAGATA Yasuo KISO Chieko KAI Midori YOSHIZAWA 《The Journal of reproduction and development》2021,67(4):265
Advanced reproductive technologies are being applied for the propagation of squirrel monkeys, to ensure their preservation as a genetic resource and the effective use of their gametes in the future. In the present study, oocytes and spermatozoa were collected from live squirrel monkeys, following which piezo intracytoplasmic sperm injection (ICSI) was performed using these gametes. Follicular development was induced by administering equine chorionic gonadotropin (eCG) containing inhibin antiserum to an immature squirrel monkey female. The unilateral ovary was excised after the administration of human chorionic gonadotropin (hCG), to induce ovulation, following which the larger developed follicular oocytes were collected. Follicular oocytes were prepared for ICSI using sperm from the epididymal tail of a unilateral testis extracted from a mature male. The embryos were continuously incubated in CMRL 1066 medium supplemented with 10% (v/v) fetal bovine serum. Embryo culture was performed with cumulus cells. Two experiments of ICSI carried out with three females resulted in 14 mature oocytes from the 49 cumulus-oocyte complexes collected and five embryos, three of which developed into blastocysts. These blastocysts were vitrified, thawed, and transferred to recipient monkeys, but no pregnancies resulted. In conclusion, the present study is the first to successfully produce ICSI-derived blastocysts from MII oocytes obtained by means of hormone administration (a combination of eCG+inhibin antiserum and hCG) and in vitro maturation in immature squirrel monkeys. 相似文献
84.
Gina Cannarozzi Solomon Chanyalew Kebebew Assefa Abate Bekele Regula Blösch Annett Weichert Dominik Klauser Sonia Plaza-Wüthrich Korinna Esfeld Moritz Jöst Abiel Rindisbacher Habte Jifar Victoria Johnson-Chadwick Ermias Abate Wuyan Wang Rizqah Kamies Negussu Husein Worku Kebede Kidist Tolosa Yazachew Genet Kidu Gebremeskel Brikti Ferede Firew Mekbib Federico Martinelli Hans Christian Pedersen Suhail Rafudeen Shimelis Hussein Muluneh Tamiru Naomi Nakayama Mike Robinson Ian Barker Samuel Zeeman Zerihun Tadele 《Euphytica》2018,214(2):31
Indigenous crops also known as orphan crops are key contributors to food security, which is becoming increasingly vulnerable with the current trend of population growth and climate change. They have the major advantage that they fit well into the general socio-economic and ecological context of developing world agriculture. However, most indigenous crops did not benefit from the Green Revolution, which dramatically increased the yield of major crops such as wheat and rice. Here, we describe the Tef Improvement Project, which employs both conventional- and molecular-breeding techniques to improve tef—an orphan crop important to the food security in the Horn of Africa, a region of the world with recurring devastating famines. We have established an efficient pipeline to bring improved tef lines from the laboratory to the farmers of Ethiopia. Of critical importance to the long-term success of this project is the cooperation among participants in Ethiopia and Switzerland, including donors, policy makers, research institutions, and farmers. Together, European and African scientists have developed a pipeline using breeding and genomic tools to improve the orphan crop tef and bring new cultivars to the farmers in Ethiopia. We highlight a new variety, Tesfa, developed in this pipeline and possessing a novel and desirable combination of traits. Tesfa’s recent approval for release illustrates the success of the project and marks a milestone as it is the first variety (of many in the pipeline) to be released. 相似文献
85.
86.
Generation of rats from vitrified oocytes with surrounding cumulus cells via in vitro fertilization with cryopreserved sperm 
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下载免费PDF全文 Katsuyoshi Fujiwara Maki Kamoshita Tsubasa Kato Junya Ito Naomi Kashiwazaki 《Animal Science Journal》2017,88(1):180-184
The objective of this study was to evaluate fertility and full‐term development of rat vitrified oocytes after in vitro fertilization (IVF) with cryopreserved sperm. Oocytes with or without surrounding cumulus cells were vitrified with 30% ethylene glycol + 0.5 mol/L sucrose + 20% fetal calf serum by using the Cryotop method. The warmed oocytes were co‐cultured with sperm. Although the denuded/vitrified oocytes were not fertilized, some of the oocytes vitrified with cumulus cells were fertilized (32.7%) after IVF with fresh sperm. When IVF was performed with cryopreserved sperm, vitrified or fresh oocytes with cumulus cells were fertilized (62.9% or 41.1%, respectively). In addition, to confirm the full‐term development of the vitrified oocytes with surrounding cumulus cells after IVF with cryopreserved sperm, 108 vitrified oocytes with two pronuclei (2PN) were transferred into eight pseudopregnant females, and eight pups were obtained from three recipients. The present work demonstrates that vitrified rat oocytes surrounded by cumulus cells can be fertilized in vitro with cryopreserved sperm, and that 2PN embryos derived from cryopreserved gametes can develop to term. To our knowledge, this is the first report of successful generation of rat offspring derived from vitrified oocytes that were fertilized in vitro with cryopreserved sperm. 相似文献
87.
Naomi Radke Rasoul Yousefpour Roderich von Detten Stefan Reifenberg Marc Hanewinkel 《Annals of Forest Science》2017,74(2):43
Key message
Multi-objective robust decision making is a promising decision-making method in forest management under climate change as it adequately considers deep uncertainties and handles the long-term, inflexible, and multi-objective character of decisions. This paper provides guidance for application and recommendation on the design.Context
Recent studies have promoted the application of robust decision-making approaches to adequately consider deep uncertainties in natural resource management. Yet, applications have until now hardly addressed the forest management context.Aims
This paper seeks to (i) assemble different definitions of uncertainty and draw recommendation to deal with the different levels in decision making, (ii) outline those applications that adequately deal with deep uncertainty, and (iii) systematically review the applications to natural resources management in order to (iv) propose adoption in forest management.Methods
We conducted a systematic literature review of robust decision-making approaches and their applications in natural resource management. Different levels of uncertainty were categorized depending on available knowledge in order to provide recommendations on dealing with deep uncertainty. Robust decision-making approaches and their applications to natural resources management were evaluated based on different analysis steps. A simplified application to a hypothetical tree species selection problem illustrates that distinct robustness formulations may lead to different conclusions. Finally, robust decision-making applications to forest management under climate change uncertainty were evaluated and recommendations drawn.Results
Deep uncertainty is not adequately considered in the forest management literature. Yet, the comparison of robust decision-making approaches and their applications to natural resource management provide guidance on applying robust decision making in forest management regarding decision contexts, decision variables, robustness metrics, and how uncertainty is depicted.Conclusion
As forest management is characterized by long decision horizons, inflexible systems, and multiple objectives, and is subject to deeply uncertain climate change, the application of a robust decision-making framework using a global, so-called satisficing robustness metric is recommended. Further recommendations are distinguished depending on the decision context.88.
89.
Acidulocomposting recycles food wastes by means of thermophilic lactic acid fermentation. This process can decrease ammonia volatilization and odor emission during processing and produce compost with high nitrogen (N) content. To compare the yield of potatoes (Solanum tuberosum L. ‘Dansyakuimo’) and the suppression of weeds with acidulocompost (AC) and those with conventional composts and inorganic fertilizer (IF), we conducted field experiments in Miyagi Prefecture, northeastern Japan. Potatoes were cultivated in 2008 and 2009 in an Andosol field treated with AC, conventional food waste compost (FWC), poultry manure compost (PMC), cattle manure compost (CMC), IF, or no fertilizer (NF). AC, but not the other treatments, delayed the emergence of potatoes, and suppressed the emergence of weeds, but it did not inhibit potato growth during the late growth stage or yield. Potato N uptake and tuber yield with AC were significantly higher than those with NF and similar to those with FWC, PMC, and IF. The N uptake efficiencies (ratio of difference between N uptake in the treatment and the control to added N) for AC (10.4–12.7% in 2008 and 2009) were similar to those for FWC and PMC (10.2–13.1%), higher than those for CMC (–1.3 to 6.3%), but significantly lower than those for IF (30.2–42.3%). Our findings indicate that AC has an N supply capacity similar to those of FWC and PMC and additionally suppresses the emergence and growth of weeds. 相似文献
90.
Quercetin exhibits a potent anticarcinogenic activity. However, ingested quercetin circulates as the glucuronide/sulfate conjugates, which are less active compared to the aglycone in healthy individuals. This study aimed to develop further understandings of the cancer-preventing mechanism with dietary quercetin. According to a two-stage hepatocarcinogenesis model with N-diethylnitrosamine (DEN) and phenobarbital (PB), preneoplasms were induced specifically in the liver of Fisher 344 rats. In the liver, glutathione S-transferase placental form (GST-P) positive foci were produced 14 weeks later. beta-Glucuronidase activity increased significantly in the liver by 1.2-fold in the DEN/PB group compared to the activity in a saline group. In the kidney, thymus, lung, heart, and plasma, the activities were similar between both groups. When quercetin was dosed intragastrically 15 min before sacrifice, the aglycone level of quercetin in liver was significantly 1.9-fold higher in the DEN/PB group than in the saline group. On the other hand, quercetin was dosed to rats 3 times a week for 14 weeks. The treatment kept the aglycone level of quercetin at a significantly higher level and tended to suppress the formation of GST-P positive foci. The increase in beta-glucuronidase activity with carcinogenesis induction became insignificant following the frequent doses of quercetin. It was considered that quercetin aglycone played a preventative role and, thus, the conjugates were converted to the active aglycone by beta-glucuronidase that was induced by the generation of preneoplasms. 相似文献