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151.
Artemia culture in the Mekong Delta, Vietnam, is becoming more and more important to satisfy the global demands of cyst production. Nevertheless, Artemia cyst productivity in the Mekong Delta has been fluctuating largely due to not only technical inefficiencies and external factors but also farms’ characteristics. This study employed a data envelopment analysis to evaluate the production efficiencies in correlation with biological and cost inputs of Artemia culture in Vinh Chau and Bac Lieu located in the Mekong Delta. The key factors affecting technical efficiency (TE) were determined using ordinary least squares regression model. The empirical results indicated that production efficiencies of farms in Vinh Chau and Bac Lieu were similar with metatechnology ratios of 0.97 and 0.96 respectively. About 62.8% of surveyed farms were operating in technically inefficient circumstances. Farmers’ experience, educational level and culture technique training participation had positive effects on TE, while climate effects and water problems have been proved to have negative effects on TE. To improve production efficiency, inefficient farms are suggested to have moderately increasing combinations of organic fertilizer, chemical and feed. The use of probiotics should be reduced as it increases production cost and affects TE. Notably, a collaboration of scientists and feed processing companies is recommended to produce a specific formulated feed for Artemia in commercial scales. This study also proposes adaptive policies for local governments and cooperatives to assist coastal famers in the Mekong Delta overcome technical inefficiencies, improve cyst productivity and obtain economic sustainability in Artemia culture.  相似文献   
152.
Streptococcus iniae is one of the most serious aquatic pathogens, causing significant economic losses in marine and freshwater species, including Asian seabass (Lates calcarifer). Controlling this gram‐positive bacterial pathogen has been an issue in aquaculture systems, due to the combined effects of aquaculture intensification and climatic impacts. To date, there have not been any genetic parameter estimates for S. iniae resistance in Asian seabass. The main aim of this study was to examine genetic variation in S. iniae resistance and its genetic correlations with growth and cannibalism in Asian seabass families produced from a breeding programme for high growth in 2016 and 2017. The study included a total of 5,835 individual fish that were offspring of 41 sires and 60 dams (31 half‐sib and 34 full‐sib families). The experimental fish were challenged by intraperitoneal injection with a volume containing 105 CFU (colony‐forming unit)/fish. Resistance to S. iniae was measured as survival rate at 6 hr, 3, 5, 7, 10 and 15 days post‐challenge test. There were significant variations in S. iniae resistance among families at different observation periods (ranging from 24.4% to 80%). Restricted maximum‐likelihood method and mixed model analysis were applied to estimate heritability for S. iniae resistance. The heritability for S. iniae resistance ranged from 7% to 18% across different statistical models used. The common full‐sib effects accounted for 0.1%–2% of the total variation in resistance to S. iniae. Genetic correlations of the S. iniae resistance at 6 hr and 3 days with later post‐challenge test periods were low to moderate. However, these estimates for S. iniae resistance between successive measurement times (5, 7, 10 and 15 days) were high and close to 1. The genetic correlations of resistance with body weights at 180, 270 and 360 days post‐hatch were not significant as well with cannibalism. It is concluded that there is substantial additive genetic variation in resistance to S. iniae, suggesting there is potential for genetic improvement of Asian seabass for resistance to S. iniae through selective breeding.  相似文献   
153.
This study determined impacts of dietary methionine concentrations at two temperatures on growth, feeding efficiency and N‐metabolites in juvenile cobia. Methionine concentrations of the experimental diets were deficient (M9; 9 g/kg), sufficient (M12; 12 g/kg) and surplus (M16, 16 g/kg). Water temperature was normal (30°C) or elevated (34°C). Twenty cobia in triplicate tanks were fed the experimental diets for 6 weeks. Both methionine and temperature affected cobia's growth and feeding efficiency. Cobia fed M9 performed lower than the fish fed M12 and M16 diets. Additionally, cobia reared at 34°C performed poorer than at 30°C, probably due to lower voluntary feed intake in the fish reared at 34°C. Protein efficiency ratio and protein productive value in cobia fed M9 diet were less than M12 or M16 diets. This was confirmed with the improved retentions of indispensable amino acids (AAs). No interactions between methionine and temperature were observed in growth and protein accretion. At 30°C, CF improved, while HSI and VSI declined upon methionine supplementation levels. Of which an interaction between temperature and methionine was present. Plasma, muscle and liver free AA and N‐metabolites were affected by methionine and temperature. Furthermore, temperature affected cobia's lipid class composition, resulting in increased phospholipids and cholesterol at 34°C.  相似文献   
154.
Two feeding experiments were conducted to evaluate if methionine is limiting in practical grow‐out diets for tilapia, Oreochromis spp. Four diets containing 32% protein and 5% lipid were designed to compare the use of diets high in dehulled solvent‐extracted soybean meal (DSESM) and expeller pressed soybean meal (EPSM) compared with a diet containing 6% fish meal (FM). Tilapia (4.78 ± 0.07 g, mean ± SD) were randomly stocked into twelve 600‐L flow‐through tanks at 20 fish per tank. After 6 wk, there were no notable trends or statistically significant differences (P > 0.05) in final mean weight, survival rate, and feed conversion ratio (FCR) among the treatments. Because results of this study indicated that DSESM could totally replace FM in practical diets for juvenile tilapia, a second batch of diets were formulated using other protein sources. Typical levels of cottonseed meal (CSM), DSESM, and meat and bone meal (MBM) were used to evaluate whether methionine could be limiting. Two basal diet formulations were tested either without or with methionine supplement (0.06/100 g diet). The first diet contained 15% CSM, 27% DSESM, and 10% MBM and the second diet contained 15% CSM and 37% DSESM. These diets contained 28% protein and 5% lipid. Tilapia (3.90 ± 0.05 g) were randomly stocked into twelve 60‐L glass aquaria of a recirculation system at 18 fish per aquarium for 5 wk and then moved to the 600‐L flow‐through tanks for five more weeks. After 10 wk, there were no statistically significant differences (P > 0.05) in final mean weight, survival rate, and FCR among the four treatments. Results of the present study indicated that DSESM and EPSM could totally replace FM’s inclusion rate in commercial diets for juvenile tilapia. Furthermore, methionine did not appear to be limiting in practical diets using typical levels of CSM, DSESM, and MBM as primary protein sources.  相似文献   
155.
Two feeding experiments were conducted to confirm methionine requirement in practical diets of juvenile Nile tilapia, Oreochromis niloticus. Test diets used in both experiments contained 414 kcal gross energy, 28 g protein, and 5 g lipid per 100 g diet. In the first experiment, seven diets were made using cottonseed meal (CSM), dehulled solvent‐extracted soybean meal (DSESM), and gelatin as intact protein sources. Methionine was added to five of these diets at 0.03 or 0.06% increments to produce methionine levels ranging from 0.33 to 0.57% of the diet. Each diet was fed to four replicate groups of male juvenile Nile tilapia (5.62 ± 0.13 g) in a recirculation system for 8 wk. Broken‐line regression analysis of weight gain indicated that methionine requirement of juvenile Nile tilapia was 0.49% of the diet or 1.75% of dietary protein at cystine level of 0.45% of the diet. The second experiment was designed based on methionine requirement determined in the first experiment and also contained seven test diets. The first six diets contained CSM and DSESM as protein sources. Methionine was added to five of these diets at an increasing rate of 0.06% to produce methionine levels ranging from 0.49 to 0.79% of the diet. In the last diet (Diet 7), a portion of DSESM was replaced by gelatin to reduce methionine level to 0.33% of the diet in order to test whether methionine is limited. Each diet was also fed to four replicate groups of male juvenile Nile tilapia (2.32 ± 0.06 g) in a recirculation system for 9 wk. At the termination of the second experiment, there were no significant differences in terms of weight gain, survival, and feed efficiency ratio (FER) among the fish fed the first six diets. However, weight gain and FER of the fish fed these diets were significantly better than those fed Diet 7, confirming the methionine requirement value as has been determined in the first experiment.  相似文献   
156.
There is growing interest in using multi-species plantation systems when undertaking reforestation for timber production. Such plantations can have ecological and socio-economic advantages over those of traditional monocultures. Despite increasing evidence about the functional advantages of increasing species richness in reforestation, there are few silvicultural guidelines to assist in the design and management of multi-species plantings. This paper presents the results of a systematic assessment of previous studies of mixed-species plantings with a particular focus on their advantages and disadvantages for meeting the needs of rural smallholders and communities in tropical regions. Research on mixed-species plantations has increased in recent years. Many earlier studies were concerned with the capacity of mixed-species plantings to improve productivity or the nutritional impacts of mixtures. Many of these studies emphasised young plantations and mixtures of a few relatively fast-growing Acacia, Eucalyptus and Pinus species. More recent studies have explored a wider range of outcomes arising from using mixtures including on the supply of ecosystem services. Issues deserving further study concern the economic advantages or disadvantages of mixed-species plantations, how these plantations might be designed to suit various environmental and socio-economic situations, and how to manage older mixed-species plantations where the interactions between species may be different to those in younger plantations. There is also a need to explore how increased species richness may affect the capacity of new plantations to withstand damage from insect pests or disturbances such as storms or wildfire.  相似文献   
157.
Forest ecosystems can modify the atmospheric CO2 through biomass accumulation mostly in tree stems with diameter at breast height (DBH) ≥ 10 cm. Aboveground biomass increment (ΔAGB), and changes in stand AGB, no. stems and basal area (BA) were calculated from mortality, recruitment, and growth data of tree stems in tropical evergreen broadleaved forest, Central Highland Vietnam. Data were derived from ten 1-ha permanent plots established in 2004, where all stems with DBH ≥ 10 cm were tagged, identified to species, and measured for DBH in 2004 and 2012. In an 8-year duration, the increment was 53 ± 10 stems ha–1, 7.8 ± 0.3 m2 ha–1 for BA and 86.0 ± 4.6 Mg ha–1 for AGB. The stem mortality rate was 0.9% year–1 and the stem recruitment rate was 2.2% year–1. Annual ΔAGB was 10.8 Mg ha–1 year–1, equaling to 5.4 Mg C ha–1 year–1. Of which, tree stems of 35–80 cm DBH classes accounted for 65%. The results indicated that the forest is in stage of carbon sequestration. Any disturbances causing death of 35–80 cm DBH tree stems will much reduce carbon sequestration capacity and it will take a long time for AGB to return to pre-disturbance stage.  相似文献   
158.
In vitro cell culture methods are crucial for the isolation, purification and mass propagation of intracellular pathogens of aquatic organisms. Cell culture infection models can yield insights into infection mechanisms, aid in developing methods for disease mitigation and prevention, and inform commercial‐scale cultivation approaches. This study details the establishment of a larval cell line (GML‐5) from the Atlantic cod (Gadus morhua) and its use in the study of microsporidia. GML‐5 has survived over 100 passages in 8 years of culture. The line remains active and viable between 8 and 21°C in Leibovitz‐15 (L‐15) media with 10% foetal bovine serum and exhibits a myofibroblast phenotype as indicated by immuno‐positive results for vimentin, α‐smooth muscle actin, collagen I and S‐100 proteins, while being desmin‐negative. GML‐5 supports the infection and development of two microsporidian parasites, an opportunistic generalist (Anncaliia algerae) and cod‐specific Loma morhua. Using GML‐5, spore germination and proliferation of L. morhua was found to require exposure to basic pH and cool incubation temperatures (8°C), in contrast to A. algerae, which required no cultural modifications. Loma morhua‐associated xenoma‐like structures were observed 2 weeks postexposure. This in vitro infection model may serve as a valuable tool for cod parasitology and aquaculture research.  相似文献   
159.
This study aimed to evaluate the effect of Lactococcus lactis K-C2 on the growth performance, microbial diversity, and release of free amino acids in the intestinal tract and the edible parts of young amberjack, Seriola dumerili. Fish were fed a diet with or without strain K-C2 (2?×?1010 cfu/g feed) for 25 days. The results indicated that the growth performance of fish in the treated group was significantly higher than those in the control group (p?<?0.05). The amount of five amino acids (aspartate, sarcosine, taurine, alanine, and arginine) in the gut content and 13 of 21 amino acids in the edible parts of fish in the treated group were significantly higher (p?<?0.05) than those in the control group. Sphingomonas, Propionibacterium, and Mycobacterium were observed in gut microflora of fish in both the control and treated groups. Staphylococcus and Kocuria were detected in one sample from the control and treated groups; Acinetobacter and Acidobacteria were found in one sample from the control group. L. lactis was only found in one sample in the treated group. In conclusion, the dietary administration of probiotic L. lactis stimulated growth, reduced feed consumption, and improved the nutritional value of cultured amberjack.  相似文献   
160.
为探究不同碳水化合物和蛋白质水平膨化饲料对大规格草鱼生长、体成分、肠道消化酶和血清指标的影响,以初始体质量(398.6±5.9)g的大规格草鱼为研究对象,设置不同碳水化合物/蛋白质水平分别为C31P30、C34P28、C37P26、C40P24、C43P22、C46P20、C49P18的7组膨化饲料,养殖周期为16周。实验结果显示,各饲料组草鱼成活率(SR)差异不显著,随着蛋白质水平的下降、碳水化合物水平的升高,增重率(WGR)呈下降趋势,蛋白质效率(PER)呈升高趋势;肝体比(HSI)、脏体比(VSI)、肠体比(ISI)、肌肉和肝脏粗脂肪(CF)含量均有随饲料碳水化合物水平升高而升高的趋势。随着饲料中碳水化合物水平升高、蛋白质水平下降,各组间草鱼肌肉粗蛋白质(CP)含量无显著差异;肝糖原、肌糖原含量呈升高趋势,饲料中碳水化合物水平分别高于46%和40%时,肝糖原、肌糖原含量显著升高。肠道胰蛋白酶(PRS)和淀粉酶(AMS)活性随饲料中碳水化合物水平的升高呈先下降后升高趋势,脂肪酶(LPS)活性呈升高趋势;血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)含量及谷草转氨酶与丙转氨酶(GOT/GPT)比值都随饲料中碳水化合物水平升高和蛋白质水平下降而呈升高的趋势。本实验结果显示:当饲料碳水化合物水平高于37%、蛋白质水平低于26%时,将显著影响草鱼的生长,饲料中高碳水化合物水平将导致鱼体肝体比、脏体比、肠体比以及肌肉和肝脏脂肪含量升高。  相似文献   
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