Maltoporin (LamB protein) contributes to the virulence and adhesion of Aeromonas veronii TH0426     

Bintong Yang  Dongxing Zhang  Tonglei Wu  Zhiqiang Zhang  Sayed Haidar Abbas Raza  Nicola Schreurs  Lei Zhang  Guilian Yang  Chunfeng Wang  Aidong Qian  Yuanhuan Kang  Xiaofeng Shan 《Journal of fish diseases》2019,42(3):379-389

Aeromonas veronii is one of the main pathogens causing freshwater fish sepsis and ulcer syndrome. More and more cases have shown that it has become an important zoonotic and aquatic agent. In this study, a A. veronii TH0426 mutant strain (ΔlamB) with an in‐frame deletion removed nucleotides 10–1,296 of the lamB gene was firstly constructed to investigate its functions. The results showed that the LD₅₀ value of the mutant ΔlamB to zebrafish and mice was 13.7‐fold and 5.6‐fold higher than those of the wild‐type strain, respectively. The toxicity of wild‐type strain to EPC cells was 2.1‐fold and threefold higher than those of ?lamB when infected for 1 and 2 hr. Furthermore, the ability of biofilm formation and the adhesion and invasion to EPC cells of ?lamB significantly decreased for 5.6‐fold and 1.8‐fold separately. In addition, motility detection result indicated that ?lamB lost the swimming ability. The results of flagellar staining and TEM demonstrated that the flagella of ?lamB were shed. In general, the deletion of lamB gene caused a significant decrease in the virulence and adhesion of A. veronii TH0426, and it can be known that the lamB gene of A. veronii plays a crucial role in the pathogenesis.  相似文献   

Length‐based assessment of larval lamprey population structure at differing spatial scales     

Samuel Shephard  Tara Gallagher  Sean M. Rooney  Nicola O'Gorman  Brian Coghlan  James J. King 《水产资源保护:海洋与淡水生态系统》2019,29(1):39-46

Interdisciplinary modeling and the significance of soil functions     

Hans‐Georg Frede  Martin Bach  Nicola Fohrer  Lutz Breuer 《植物养料与土壤学杂志》2002,165(4):460-467

  相似文献   

With or without pheromone habituation: possible differences between insect orders?          下载免费PDF全文

David Maxwell Suckling  Lloyd D Stringer  Alfredo Jiménez‐Pérez  Gimme H Walter  Nicola Sullivan  Ashraf M El‐Sayed 《Pest management science》2018,74(6):1259-1264

  相似文献   

Bridging the divide: Social–ecological coherence in Marine Protected Area network design          下载免费PDF全文

Sian E. Rees  Simon J. Pittman  Nicola Foster  Olivia Langmead  Charly Griffiths  Steve Fletcher  David E. Johnson  Martin Attrill 《水产资源保护:海洋与淡水生态系统》2018,28(3):754-763

Non-crop plants used as hosts by Drosophila suzukii in Europe     

Marc Kenis  Lorenzo Tonina  René Eschen  Bart van der Sluis  Manuel Sancassani  Nicola Mori  Tim Haye  Herman Helsen 《Journal of pest science》2016,89(3):735-748

  相似文献   

Laboratory and field assessment of cyantraniliprole relative to existing fly baits          下载免费PDF全文

Amy C Murillo  Alec C Gerry  Nicola T Gallagher  Nyles G Peterson  Bradley A Mullens 《Pest management science》2015,71(5):752-758

  相似文献   

Weak effects of habitat type on susceptibility to invasive freshwater species: an Italian case study          下载免费PDF全文

Angela Boggero  Alberto Basset  Martina Austoni  Enrico Barbone  Luca Bartolozzi  Isabella Bertani  Alessandro Campanaro  Antonella Cattaneo  Fabio Cianferoni  Giuseppe Corriero  Ambrosius Martin Dörr  A. Concetta Elia  Gentile Francesco Ficetola  Lyudmila Kamburska  Gianandrea La Porta  Sara Lauceri  Alessandro Ludovisi  Elda Gaino  Enzo Goretti  Massimo Lorenzoni  Marina Manca  Aldo Marchetto  Giuseppe Morabito  Francesco Nonnis Marzano  Alessandro Oggioni  Cataldo Pierri  Nicoletta Riccardi  Giampaolo Rossetti  Nicola Ungaro  Pietro Volta  Silvia Zaupa  Diego Fontaneto 《水产资源保护:海洋与淡水生态系统》2014,24(6):841-852

Competition for shifting resources in the southern Benguela upwelling: Seabirds versus purse-seine fisheries     

Nicola C. Okes  Philip A.R. Hockey  Lorien Pichegru  Carl D. van der Lingen  Robert J.M. Crawford  David Grmillet 《Biological conservation》2009,142(10):2361-2368

In the southern Benguela upwelling ecosystem off the west coast of South Africa, seabird populations are decreasing dramatically because of reduced availability of pelagic fish. We tested the hypothesis that the west coast fishing industry is competing for the remaining stocks of anchovy and sardine with the largest colony of Vulnerable Cape Gannets (Morus capensis) along the Atlantic coast. Using GPS-tracking of the birds, echo-sounding of pelagic fish, and vessel log books, we located overlap areas between bird foraging ranges, pelagic fish distribution, and fishing activities. We then compared fish catches by gannets and vessels within their joint foraging zones. In October 2007, purse-seine fishing grounds and gannet foraging areas overlapped by only 13%. However, for a 1-month period, the amount of fish removed from this area by purse-seine boats amounted to 41% of the food requirements of the 72,000 gannets breeding on Malgas Island (25% of the world population). The fishery’s catch in this area is significant in terms of its potential impact on gannets, but comprises only 3.6% of total fishery catch. Based on this finding, the rapidly decreasing size of the gannet colony and the stated objectives of South Africa’s Marine Living Resources Act of 1998, the case for considering and experimenting with at-sea ‘no-take’ areas for the purse-seine fishery is strong. Efforts to establish whether ‘no-take’ fishing zones increase food availability for top predators is an important next step in conservation of the southern Benguela Ecosystem.  相似文献   

Brightness of venous blood in South American camelids: implications for jugular catheterization     

Nicola Grint BVSc  DVA  Diplomate ECVAA  MRCVS  & Alexandra Dugdale MA  Vet MB  DVA  Diplomate ECVAA  MRCVS 《Veterinary anaesthesia and analgesia》2009,36(1):63-66

Objective  To compare the brightness of South American camelid venous blood to that of Equidae.
Study design  Prospective clinical evaluation.
Animals  Twelve South American camelids (eight llamas, four alpacas), eight horses and ponies (control group).
Methods  Appropriately sized catheters were placed in the jugular vein of each animal under local anaesthesia. The blood spilt before the catheter was capped was caught on a white tile. A sample of blood was drawn for blood-gas analysis. The brightness of the blood (both on the tile and in the syringe) was matched to a colour chart (1 = darkest red, 8 = brightest red) by a single observer under bright light conditions. Packed cell volume (PCV) and partial pressure of oxygen (PvO₂) in the blood were also measured on the syringe blood. Normally distributed data were compared using a two tailed t -test, and non-normally distributed data were compared using a Mann–Whitney U -test. Significance was set at p  < 0.05.
Results  Camelid venous blood was significantly brighter red than that of horses and ponies both on the white tile ( p  = 0.0003) and in the syringe ( p  = 0.0001). PCV was significantly lower in camelids (32 ± 4%) compared with horses (37 ± 5%). Partial pressure of oxygen values were similar between groups.
Conclusions and clinical relevance  Jugular venous blood in alpacas and llamas is significantly brighter red than that of horses. Colour should not be used as a sole determinant of venous or arterial catheterization in this species.  相似文献