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21.
Alessio Bonaldo Gloria Isani Ramon Fontanillas Luca Parma Ester Grilli Pier Paolo Gatta 《Aquaculture International》2010,18(5):909-919
Three isoproteic (47% protein) diets were formulated to contain graded levels of crude fat (diet D16:16%, diet D24: 24% and
diet D32: 32%). Each diet was fed to satiation in three and to 80% satiation in two replicate groups of gilthead sea bream
(Sparus aurata), having an initial body weight of 72–74 g. The trial lasted 81 days. Groups fed to satiation showed higher final body weight
(FBW; 238.8–252.3 g vs. 218.0–229.3 g) and daily growth index (DGI; 2.49–2.65%/day vs. 2.27–2.34%/day) than those fed to 80%
satiation. Feed intake was significantly different both for feeding level and for diet composition. Fish fed to satiation
had higher feed conversion rate (FCR) compared to the 80% satiation groups (1.33–1.44 vs. 1.13–1.17; P ≤ 0.001). Within satiation groups, FCR was significantly lower in fish fed D16 compared to fish fed D32 (1.33 vs. 1.44, P ≤ 0.05), whereas no statistical differences were found within the 80% satiation groups. The increase in dietary lipid level
did not improve growth performance, feed efficiency and protein utilization but decreased gross lipid efficiency. Conversely,
a reduction in ration from satiation to 80% satiation decreased DGI, thus improving FCR. Feed costs were influenced by dietary
energy level and feeding ratio, the lowest energy diet at 80% satiation being the most profitable combination among the variables. 相似文献
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Vittori Antisari Livia Bianchini Gianluca Cremonini Stefano Di Giuseppe Dario Falsone Gloria Marchesini Marco Marvelli Silvia Vianello Gilmo 《Journal of Soils and Sediments》2016,16(2):645-662
Journal of Soils and Sediments - This study investigated a Lateglacial to Holocene sedimentary sequence derived from a small catchment located at San Lazzaro di Savena in the surroundings of... 相似文献
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Nidhi Rawat Vijay K. Tiwari Neelam Singh Gursharn S. Randhawa Kuldeep Singh Parveen Chhuneja Harcharan S. Dhaliwal 《Genetic Resources and Crop Evolution》2009,56(1):53-64
Grains of 80 accessions of nine species of wild Triticum and Aegilops along with 15 semi-dwarf cultivars of bread and durum wheat grown over 2 years at Indian Institute of Technology, Roorkee,
were analyzed for grain iron and zinc content. The bread and durum cultivars had very low content and little variability for
both of these micronutrients. The related non-progenitor wild species with S, U and M genomes showed up to 3–4 folds higher
iron and zinc content in their grains as compared to bread and durum wheat. For confirmation, two Ae. kotschyi Boiss. accessions were analyzed after ashing and were found to have more than 30% higher grain ash content than the wheat
cultivars containing more than 75% higher iron and 60% higher zinc than that of wheat. There were highly significant differences
for iron and zinc contents among various cultivars and wild relatives over both the years with very high broad sense heritability.
There was a significantly high positive correlation between flag leaf iron and grain iron (r = 0.82) and flag leaf zinc and grain zinc (r = 0.92) content of the selected donors suggesting that the leaf analysis could be used for early selection for high iron
and zinc content. ‘Chinese Spring’ (Ph
I
) was used for inducing homoeologous chromosome pairing between Aegilops and wheat genomes and transferring these useful traits from the wild species to the elite wheat cultivars. A majority of
the interspecific hybrids had higher leaf iron and zinc content than their wheat parents and equivalent or higher content
than their Aegilops parents suggesting that the parental Aegilops donors possess a more efficient system for uptake and translocation of the micronutrients which could ultimately be utilized
for wheat grain biofortification. Partially fertile to sterile BC1 derivatives with variable chromosomes of Aegilops species had also higher leaf iron and zinc content confirming the possibility of transfer of required variability. Some of
the fertile BC1F3 and BC2F2 derivatives had as high grain ash and grain ash iron and zinc content as that of the donor Aegilops parent. Further work on backcrossing, selfing, selection of fertile derivatives, leaf and grain analyses for iron and zinc
for developing biofortified bread and durum wheat cultivars is in progress.
Nidhi Rawat, Vijay K. Tiwari, and Neelam Singh have contributed equally to the work. 相似文献
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Anil Kumar Nidhi Gupta Atul Kumar Gupta Vikram Singh Gaur 《Journal of Crop Science and Biotechnology》2009,12(4):183-194
Worldwide, the nitrogen use efficiency (NUE) for crop plants is of great concern. The burgeoning world population needs crop genotypes that respond to higher nitrogen and show a direct relationship to yield with use of nitrogen inputs, i.e. high nitrogen-responsive genotypes. However, for fulfilling the high global demand of organic produce, it requires the low nitrogen responsive genotypes with greater NUE and grain yields. The lack of knowledge about precise regulatory mechanisms to explain NUE in crop plants hampers the goal of agricultural productivity. Understanding the molecular basis of NUE will enable to provide handle for crop improvement through biotechnological means. With the advent of modern genomics and proteomics approaches such as subtractive hybridization, differential display, and microarray techniques are revolutionizing to identify the candidate genes which play a pivotal role in the regulation of NUE. Beside it, quantitative real-time polymerase chain reaction technology is also being used to establish marker-trait association for NUE. The identification of potential candidate genes/proteins in the regulation of NUE will serve as biomarker(s) for screening genotypes for their nitrogen responsiveness for optimization of nitrogen input in agriculture. This paper describes the molecular basis of NUE with respect to nitrogen metabolism and its intimate relationship with carbon metabolism, use of molecular-physiological-genetics approaches for understanding the role of various genes/proteins, and their validation to use as biomarker(s) for determining genotypic potential for NUE. Since NUE in plants is a complex trait which not only involves the primary process of nitrogen uptake and assimilatory pathways but also a series of events, including metabolite partitioning, secondary remobilization, C-N interactions, as well as molecular signalling pathways and regulatory control outside the metabolic cascades. Therefore, identification of novel nitrogen responsive genes and their cis- and trans-acting gene elements is essential. Thus, fishing out a single gene, biomarker or a master regulator controlling complex trait of NUE could serve as an appropriate strategy for nitrogen management in agriculture. 相似文献
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Angela Briganti DMV PhD Pierre Melanie DMV PhD Diego Portela DMV PhD Gloria Breghi DVM Khursheed Mama DVM DACVA 《Journal of Veterinary Emergency and Critical Care》2010,20(5):503-508
Objective – To evaluate the tolerance of a continuous positive airway pressure (CPAP) mask in tranquilized dogs and compare PaO2 in arterial blood in dogs receiving oxygen with a regular face mask or CPAP mask set to maintain a pressure of 2.5 or 5 cm H2O. Design – Prospective, randomized clinical study. Setting – University teaching hospital. Animals – Sixteen client‐owned dogs without evidence of cardiopulmonary disease were studied. Interventions – Eight animals were randomly assigned to each of 2 treatment groups: group A received 2.5 cm H2O CPAP and group B received 5 cm H2O CPAP after first receiving oxygen (5 L/min) by a regular face mask. Animals were tranquilized with acepromazine 0.05 mg/kg, IV and morphine 0.2 mg/kg, IM. An arterial catheter was then placed to facilitate blood sampling for pHa, PaO2, and PaCO2 determinations before and after treatments. Direct mean arterial pressure, heart rate, respiratory rate, and temperature were also recorded after each treatment. Measurements and Main Results – CPAP administration was well tolerated by all animals. The mean arterial pressure, heart rate, respiratory rate, temperature, PaCO2, and pHa, did not differ at any time point between groups. Differences were seen in oxygenation; in group A, PaO2 significantly increased from a mean of 288.3±47.5 mm Hg with a standard mask to a mean of 390.3±65.5 mm Hg with the CPAP mask and in group B, PaO2 increased similarly from 325.0±70.5 to 425.2±63.4 mm Hg (P<0.05); no differences were detected between the 2 CPAP treatments. Conclusions – In healthy tranquilized dogs noninvasive CPAP is well tolerated and increases PaO2 above values obtained when using a regular face mask. 相似文献
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