Changes in follicular vascularity during the first follicular wave in lactating cows     

Acosta TJ  Hayashi KG  Matsui M  Miyamoto A 《The Journal of reproduction and development》2005,51(2):273-280

Increase in the blood supply to individual follicles appears to be associated with follicular growth rates and the ability to become the dominant follicle, while reduced thecal vascularity appears to be closely associated with follicular atresia. Therefore, this study aimed to determine the real-time changes in the vascularity of the follicle wall during the first follicular wave in cycling Holstein cows. Normally cycling and lactating cows (n=5) were examined by transrectal color Doppler ultrasonography (the sensitivity for velocity: > 2 mm/sec) to determine the changes in the vasculature of the follicle wall (presence or absence of blood flow) and the diameter of follicles. A new follicular wave and ovulation were induced by GnRH injection at 48 h after an injection of PGF2alpha analogue. The ovaries were scanned daily for 7 days after GnRH injection. Follicles >2.5 mm were classified into 3 groups by the changes in diameter as follows: 1) largest follicle, 2) second largest follicle, and 3) small follicles, which included all other follicles >2.5 mm. Before the follicle selection, there was no significant difference in the percentage of follicles with detectable blood flow between the subsequently determined largest and second largest follicles. After the follicle selection, the percentage of follicles with detectable blood flow significantly decreased among the second largest follicles. In addition, small follicles with detectable blood flow kept larger diameters than those without detectable blood flow from one day before the occurrence of follicle selection. It is likely that maintenance of follicle vasculature and appropriate blood supply to the larger follicles is essential for follicle dominance. In small follicles, the presence of blood flow within the wall also appears to be required for recruitment. Consequently, the data suggest that the change of the blood supply to an individual follicle closely relates to the dynamics of follicular growth in the first follicular wave in the cow.  相似文献   

Single-step purification and evaluation of recombinant BP26 protein for serological diagnosis of Brucella ovis infection in rams   总被引：1，自引：0，他引：1  

Zygmunt MS  Baucheron S  Vizcaino N  Bowden RA  Cloeckaert A 《Veterinary microbiology》2002,87(3):213-220

To investigate the value of the BP26 protein in the serological diagnosis of ovine brucellosis caused by Brucella ovis, recombinant BP26 protein was produced in Echerichia coli and purified for use in an indirect enzyme-linked immunosorbent assay (I-ELISA). The majority of the recombinant protein was recovered from the supernatant of sonicated recombinant E. coli cells in a soluble form. This facilitated the purification of the recombinant BP26 protein which was achieved by using ion-exchange chromatography. After one step of purification, the purity of the recombinant BP26 protein was analyzed by using SDS-PAGE, Coomassie blue staining, and Western blot with a monoclonal antibody (MAb) directed against the BP26 protein. The degree of purity appeared satisfactory so that it could be directly used in I-ELISA. Although the recombinant BP26-ELISA appeared less useful than I-ELISA using the B. ovis hot saline (HS) extract as antigen, the high number of sera from B. ovis infected rams found positive (90%) in the recombinant BP26-I-ELISA indicated that the BP26 protein may be an additional suitable antigen for serological diagnosis of B. ovis infection in rams.  相似文献   

Effects of LH and PGF2alpha in equine dominant follicles observed by MDS     

Kojima AY  Kobayashi S  Acosta TJ  Kudo M  Miyamoto A  Takagi M  Miyazawa K  Sato K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(2):119-122

Microdialysis System (MDS) is a novel technique used for investigation of molecule secretion between different cell populations. Local hormonal secretion at follicular wall has been still unclear. This MDS study was used to determine progesterone (P4), androstenedione (A4), estradiol-17beta (E2) and Prostaglandin F2alpha (PGF2alpha) release in mare pre-ovulatory follicles. Follicles larger than 30 mm were isolated from the ovary and follicular fluid aspirated for hormone assay. Follicular fluid collected from small, middle and large follicles were analyzed by EIA. The concentrations of P4 and PGF2alpha were similar among the different sizes of follicles. The release of A4 was observed in middle and large follicles. E2 concentration was observed in middle follicles and was higher in large follicles compared with middle follicles. Follicular wall was cut and incubated for MDS and when LH was infused, there was an increase in P4 and A4 release. PGF2alpha release was considerably high after LH infusion compared to the control group. Infusion of PGF2alpha increased P4 and A4 release but there was no change in E2 release. This results suggest that in pre-ovulatory follicles, LH stimulates theca interna cells and also PGF2alpha seemed to have a mediator role to induce steroid hormone production and luteinization of follicular cells. The nature of the mechanisms involved in selection of large follicles is still a perplexing research problem in reproduction.  相似文献   

Glucosinolates and Isothiocyanates from Moringa oleifera: Chemical and Biological Approaches     

Lopez-Rodriguez  Norma A.  Gaytán-Martínez  Marcela  de la Luz Reyes-Vega  María  Loarca-Piña  Guadalupe 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(4):447-457

Plant Foods for Human Nutrition - Alternative therapies, such as phytotherapy, are considered to improve the health status of people with chronic non-communicable diseases (CNCDs). In this regard,...  相似文献   

Influence of hatchery rich‐carbohydrate diet on the oyster Crassostrea corteziensis (Hertlein, 1951) farming     

Jos Manuel Mazn‐Sustegui  Guadalupe Armando Leyva‐Miranda  Dwight Arrieche‐Galíndez  Csar Lodeiros‐Seijo  Jesús Antonio Lpez‐Carvallo 《Aquaculture Research》2019,50(10):3078-3081

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First report of Streptococcus iniae in red porgy (Pagrus pagrus, L.)     

El Aamri F  Padilla D  Acosta F  Caballero M  Roo J  Bravo J  Vivas J  Real F 《Journal of fish diseases》2010,33(11):901-905

This work describes the first isolation of Streptococcus iniae in red porgy, Pagrus pagrus (L.), and the first European isolation of this pathogen in gilthead seabream, Sparus aurata (L.). In both farmed fish species, infection resulted in lethargy, anorexia, abnormal swimming, exophthalmia and sudden death, with mortality rates of over 25% in red porgy and 10% in gilthead seabream. Beta-haemolytic Gram-positive cocci, catalase negative and oxidase negative, were isolated in pure culture from internal organs. Conventional and rapid identification systems, and 16S rRNA gene partial sequencing were used to identify the causative agent of the natural disease. LD50 trials were carried out to show the virulence of this isolated strain in these species, with values of 1.7 × 104 CFU per fish in red porgy and 1.32 × 105 CFU per fish in gilthead seabream. The most prominent lesions were meningoencephalitis and multifocal infiltration of macrophage cells in the kidney and spleen.  相似文献   

Synthesis of new tetronamides displaying inhibitory activity against bloom‐forming cyanobacteria     

Jaime AM Acosta  Milandip Karak  Luiz CA Barbosa  John Boukouvalas  Andrea Straforini  Giuseppe Forlani 《Pest management science》2020,76(2):779-788

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Gross Morphology of Diseased Tissues in Nezara viridula (Hemiptera: Pentatomidae) and Molecular Characterization of an Associated Microsporidian     

Adam R Rivers  Michael J Grodowitz  Godfrey P Miles  Margaret L Allen  Brad Elliott  Mark Weaver  Marie-Claude Bon  M Guadalupe Rojas  Juan Morales-Ramos 《Journal of insect science (Online)》2022,22(2)

Nezara viridula (L.) (Hemiptera: Pentatomidae), commonly known in the U.S. as the southern green stink bug (SGSB), is a cosmopolitan, highly polyphagous feeder that causes severe damage to a wide range of agronomically important crops such as fruit, vegetable, grain, tobacco, and cotton, throughout much of the United States, and is a global pest of considerable ecological, agricultural, and economical interest. During dissection of female Nz. viridula, conspicuous black and brown spots or lesions were observed on various internal organs. To determine the cause of these spots or lesions, tissues of fat body, spermatheca, ovaries, and ovulated eggs were collected from healthy and infected individuals. The gross morphology of the spots was characterized, and the microorganisms associated with the infection were identified by amplicon sequencing of the V4 region of the small subunit rRNA gene. The presence of a microsporidian pathogen Nosema maddoxi, Becnel, Solter, Hajek, Huang, Sanscrainte, & Estep (Microsporidia: Nosematidae) which has been observed on other species of stink bug, was evidenced for the first time. The characterization of the gross morphology of this associated microsporidian may enable more rapid determination of microsporidia infection in stink bug colonies and field populations.  相似文献   

New records of Rhipicephalus linnaei infected by Rickettsia massiliae from Central Mexico     

José Francisco Nieto-Cabrales  Beatriz Salceda-Sánchez  Héctor M. Zazueta-Islas  Marlene Solís-Cortés  Magaly Guadalupe Landa-Flores  Juan Carlos del Mazo-López  Laura Valtierra-Alzaga  Juan J. Soto-Gutiérrez  Heron Huerta-Jimenez  Ingeborg Becker  Jorge J. Rodríguez-Rojas  Sokani Sánchez-Montes 《Zoonoses and public health》2024,71(2):217-224

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Effects of prostaglandin F(2alpha) and nitric oxide on the secretory function of bovine luteal cells   总被引：1，自引：0，他引：1  

Korzekwa A  Jaroszewski JJ  Bogacki M  Deptula KM  Maslanka TS  Acosta TJ  Okuda K  Skarzynski DJ 《The Journal of reproduction and development》2004,50(4):411-417

The objective of the present study was to investigate the influence of prostaglandin F(2alpha) (PGF (2alpha)) and nitric oxide (NO) on production of steroids and PGs by culturing bovine luteal cells obtained from ovaries on days 8-12 of the estrous cycle with a nitric oxide (NO) donor (Spermine NONOate), and a NO synthase inhibitor (N(G)-nitro-L-arginine methyl ester dihydrochloride: L-NAME). When the cells were exposed for 24 h to PGF(2alpha) (10(-7)-10(-5) M), production of progesterone (P(4)) increased significantly at all doses used (P<0.05). Moreover, PGF(2alpha) stimulated PGF(2alpha) production (P<0.01), depressed testosterone (T) production (P<0.05), but did not affect synthesis of prostaglandin E(2) (PGE(2)). Spermine NONOate decreased P(4) production to 66%, 47% and 34% of the control concentration after treatment with 10(-5) M, 10(-4) M and 10(-3) M, respectively, but did not affect T production, and increased PGF(2alpha) synthesis (P<0.05) and PGE(2) (P<0.01) at all doses used. L-NAME increased production of P(4) (P<0.01) but did not affect (P>0.05) secretion of T, PGF(2alpha) and PGE(2). Estradiol-17beta (E(2)) was detectable on the level of sensitivity of assay and was not significantly altered by any treatments. The overall results suggest that PGF(2alpha) and NO produced locally in bovine CL play roles in the regulation of the secretory function of the bovine CL as auto/paracrine factors.  相似文献