Effect of hCG Stimulation on Plasma Androstenone Concentrations and Cytochrome b5 Levels in Testicular Tissue     

G Zamaratskaia  Y Lou  G Chen  Ø Andresen  K Lundström  EJ Squires 《Reproduction in domestic animals》2007,42(1):105-108

The effect of Human chorionic gonadotropin (hCG) stimulation on the concentrations of free and conjugated androstenone in plasma was studied in 34 crossbred entire male pigs (Landrace x Swedish Yorkshire). Seventeen pigs were treated with hCG 4 days prior to slaughter and the remaining pigs were treated with sterile saline and served as controls. Blood samples were taken prior to hCG or saline injection and on the day before slaughter and analysed for concentrations of free and conjugated androstenone. Testicular tissue samples were taken at slaughter and analysed for the levels of cytochrome b5 (cyb5) protein. Here we have demonstrated for the first time that hCG stimulation causes an increase in the plasma levels of both free and conjugated androstenone. Not all animals responded in the same way to hCG treatment regarding levels of free and conjugated androstenone demonstrating that individual animals can have differences in their capacity to produce free and conjugated androstenone. We suggest that hCG treatment is a good way to determine the potential for androstenone conjugation when androstenone synthesis in the testis is high. The levels of cyb5 protein in the testis were slightly related (r=0.41, p < 0.10) to free androstenone levels in the pigs after hCG administration, although levels of cyb5 protein were not affected by hCG treatment.  相似文献   

Ultrasonographic Characteristics of Ovulatory Follicles and Associated Endocrine Changes in Cyclic Ewes Treated with Medroxyprogesterone Acetate (MAP)-releasing Intravaginal Sponges and Equine Chorionic Gonadotropin (eCG)   总被引：2，自引：0，他引：2  

X Liu  Q Dai  EJ Hart  DMW Barrett  NC Rawlings  RA Pierson  PM Bartlewski 《Reproduction in domestic animals》2007,42(4):393-401

The aim of this study was to assess the ultrasonographic characteristics of ovulatory follicles in cyclic Western White Face ewes (December) that had received intravaginal sponges containing medroxyprogesterone acetate (MAP; 60 mg) for 12 days, with or without an injection of 500 IU of equine chorionic gonadotropin (eCG) at sponge removal. We hypothesized that quantitative echotextural attributes of the follicles in ewes treated only with MAP would differ from those in MAP/eCG-treated ewes, reflecting the increased antral follicular growth and secretory function under eCG influence. Digital images of ovulatory follicles obtained at 0 and 24 h after MAP sponge removal and at 24 h before ovulation in the eCG-treated (five ewes, 13 follicles) and control (six ewes, 9 follicles) animals, were subjected to computerized analyses. The mean diameter of ovulatory follicles increased (p < 0.001) 24 h after eCG treatment. The mean pixel intensity and heterogeneity of the follicular antrum (p < 0.001), as well as mean pixel intensity of the follicular wall and perifollicular ovarian stroma (p < 0.05), were greater in eCG-treated animals compared with control ewes 24 h after sponge removal and at 24 h before ovulation. Mean serum concentrations of oestradiol-17beta tended to increase (p = 0.06) 24 h after eCG treatment and the eCG-treated ewes exceeded (p < 0.05) control animals in progesterone concentrations from days 9-15 after ovulation. Our results support the hypothesis that large antral follicles in eCG-treated ewes exhibit distinctive echotextural characteristics. Follicular image attributes in eCG-treated ewes appear to be indicative of the changes in follicular morphology and secretory activity caused by the administration of the exogenous gonadotropin, which has both FSH- and LH-like activities.  相似文献   

Selection strategy for a seedling seed orchard design based on trait selection index and genomic analysis by molecular markers: a case study for Eucalyptus dunnii     

Zelener N  Marcucci Poltri SN  Bartoloni N  López CR  Hopp HE 《Tree physiology》2005,25(11):1457-1467

Molecular genetic analysis was applied to 162 individuals of 37 half-sib selected families belonging to six provenances of Eucalyptus dunnii Maiden in a provenance/family trial. The individuals were selected by a trait selection index and genetic diversity criteria were later applied for designing seedling seed orchards. Genetic diversity and its distribution, as well as relationships among individuals, were assessed on the basis of nine microsatellite loci and 243 amplified fragment length polymorphism markers. High diversity was found with both kinds of markers. Clear-cut genomic patterns of identification (fingerprinting) were obtained for each individual. Genetic differentiation estimates consistently showed low differentiation among provenances (R(ST1) = 0.069, theta(P) = 0.026 and F(CT) = 0.035) and great differentiation among families (R(ST2) = 0.223, theta(S) = 0.174 and F(SC) = 0.164). A high proportion of the total variation was observed within families (around 80% by both marker analyses), suggesting that orchard design should be based on individual or family selection rather than on provenance selection, and that individual ranking by both trait selection index and molecular genetic diversity criteria should be considered. A selection procedure for a seedling seed orchard design is proposed.  相似文献   

The effect of tricaine on use of the fluorescein test for detecting skin and corneal ulcers in fish     

Davis MW  Stephenson J  Noga EJ 《Journal of aquatic animal health》2008,20(2):86-95

Fluorescein has been used for rapid and sensitive detection of fish skin and corneal ulceration. Effective use of the fluorescein test requires knowledge of conditions that might cause misleading interpretations or otherwise interfere with test reliability. Examination of fish health and the clinical workup often require tricaine as one of the most commonly used anesthetics. However, tricaine may interfere with correct interpretation of the fluorescein test and might also cause significant fish injury. The effects of tricaine exposure sequence on the fidelity of the fluorescein test was studied in Pacific halibut Hippoglossus stenolepis, walleye pollock Theragra chalcogramma, and northern rock soles Lepidopsetta polyxystra by examining the fluorescence of experimentally induced epidermal wounding. Tricaine can quench fluorescence that is emitted by fluorescein retained in skin ulcers, causing a false-negative reaction. Thus, for the fluorescein test to work properly, it is important to avoid the exposure of fluorescein-treated and rinsed ulcers to tricaine. The effects of exposure to buffered versus unbuffered tricaine on epidermal and corneal integrity were studied in Nile tilapia Oreochromis niloticus and channel catfish Ictalurus punctatus subjected to the fluorescein test and histological examination. Fluorescein could detect not only ulcers but also areas with only a partial loss of epithelium (i.e., erosion). The use of unbuffered tricaine to anesthetize these fish caused serious epidermal and corneal damage. If fish are euthanized with unbuffered tricaine for clinical workup, this severe epidermal or corneal damage could be misinterpreted as an antemortem lesion, leading to misdiagnosis. Even in water with alkalinity exceeding 50 mg/L as CaCO3, it would seem prudent to always buffer tricaine with sodium bicarbonate to prevent a pH change that might lead to iatrogenic effects from unbuffered tricaine. Thus, current general recommendations suggesting that tricaine does not need to be buffered in waters with alkalinity greater than 50 mg/L might need to be modified.  相似文献   

Investigation of the effect of Equivac® HeV Hendra virus vaccination on Thoroughbred racing performance          下载免费PDF全文

K Schemann  EJ Annand  PA Reid  MF Lenz  PC Thomson  NK Dhand 《Australian veterinary journal》2018,96(4):132-141

Objective

To evaluate the effect of Equivac® HeV Hendra virus vaccine on Thoroughbred racing performance.

Design

Retrospective pre‐post intervention study.

Methods

Thoroughbreds with at least one start at one of six major south‐eastern Queensland race tracks between 1 July 2012 and 31 December 2016 and with starts in the 3‐month periods before and after Hendra virus vaccinations were identified. Piecewise linear mixed models compared the trends in ‘Timeform rating’ and ‘margin to winner’ before and after initial Hendra virus vaccination. Generalised linear mixed models similarly compared the odds of ‘winning’, ‘placing’ (1st–3rd) and ‘winning any prize money’. Timeform rating trends were also compared before and after the second and subsequent vaccinations.

Results

Analysis of data from 4208 race starts by 755 horses revealed no significant difference in performance in the 3 months before versus 3 months after initial Hendra vaccination for Timeform rating (P = 0.32), ‘Margin to winner’ (P = 0.45), prize money won (P = 0.25), wins (P = 0.64) or placings (P = 0.77). Further analysis for Timeform rating for 7844 race starts by 928 horses failed to identify any significant change in Timeform rating trends before versus after the second and subsequent vaccinations (P = 0.16) or any evidence of a cumulative effect for the number of vaccines received (P = 0.22).

Conclusion

No evidence of an effect of Hendra virus vaccination on racing performance was found. The findings allow owners, trainers, industry regulators and animal health authorities to make informed decisions about vaccination.  相似文献   

Tsetse and trypanosomiasis survey of southern darfur province,Sudan     

M. J. R. Hall  S. M. Kheir  A. H. A. Rahman  S. Noga 《Tropical animal health and production》1984,16(3):127-140

Summary A tsetse survey of Southern Darfur province, Sudan showed that the distribution of the only species present,Glossina morsitans submorsitans, had not appreciably altered over 10 years. Fly populations are most dense south of the Wadi Umbelasha but light infestations are found in the woodlands north of this riverine system to a latitude of about 10° 15 N. Data are given on host availability which is thought to be the major factor determining tsetse distribution. The relative importance of tsetse and of tabanids as transmitters of bovine trypanosomiasis in the province is discussed. The evidence indicates that tsetse are very much more important and that cattle are most at risk of contracting infections during their dry season southerly migrations to the tsetse belts especially during their Rushash migration at first rains. Possibilities of tsetse control are discussed but in the short term trypanosomiasis control is most likely best achieved by chemical prophylaxis/therapy of cattle at risk.

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Vigilancia Epidemiologica De La Mosca Tsetse Y De La Tripanosomiasis En La Provincia Surena De Darfur, Sudan. II. Aspectos Entomologicos

Resumen La vigilancia epidemiológica de la mosca tsetse en la provincia sureña de Darfur, Sudán, indicó que la distribución de la única especie presenteGlossina morsitans submorsitans, no se había alterado en diez años. Las poblaciones de moscas son más densas al sur de Wadi Umbelasha y ligeras en los bosques, al norte de este sistema ribereño, hasta una latitud de cerca de 10° 15 N. Se incluyen datos sobre la distribución de huespedes, los cuales determinan en parte la distribución de la mosca. Se discute tambien la importancia relativa de la mosca tsetse y de los tábanos, como vectores de la tripanosomiasis. La evidencia indica, que la mosca tsetse es el más importante vector y que el ganado se encuentra más expuesto de contraer la enfermedad, cuando migra hacia el sur hacia el cinturon de tsetse, especialmente la migración Rushash, cuando cáen las primeras lluvias. Se discuten posibilidades para el control de la mosca, pero tal parece, que el control más eficiente y a corto plazo, depende de la profilaxis química y la terápia del ganado en peligro.

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Enquete Sur Les Glossines Et La Trypanosomose Dans La Partie Sud De La Province De Darfur Au Soudan

Résumé Une enquête sur les glossines de la partie Sud de la province de Darfur au Soudan a montré que la répartition de la seule espèce présente,Glossina morsitans submorsitans, ne s'était pas modifiée de façon appréciable durant les dix dernières années. Les populations de mouches sont très denses au Sud de l'Ouadi d'Umbalasha mais des infestations légères sont trouvées dans les régions forestières au Nord de ce réseau fluvial jusqu'à une latitude d'environ 10° 15 N. Des données sont fournies sur la présence des hôtes nourriciers dont on pense qu'ils jouent un rôle déterminant dans la répartition des glossines. La relative importance des glossines et des tabanidés comme vecteur de la trypanosomose bovine dans la province est discutée. Tout concourt à démontrer que les glossines jouent un rôle beaucoup plus important et que les bovins sont les plus susceptibles de contracter des infections pendant leur migration de saison sèche vers le Sud vers les zones à glossines, spécialement pendant la migration Rushash à l'époque des premières pluies. Les possibilités de lutte contre les glossines sont discutées mais à court terme la lutte contre la trypanbosomose est très vraisemblablement mieux menée par la prophylaxie et la thérapie chimique des bovins à risques.

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Factors associated with fertility of nulliparous dairy heifers following a 10‐day fixed‐time artificial insemination program with sex‐sorted and conventional semen          下载免费PDF全文

EJ Noonan  JC Kelly  DS Beggs 《Australian veterinary journal》2016,94(5):145-148

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Cladosporiosis in a tomato clownfish (Amphiprion frenatus).     

U Silphaduang  K Hatai  S Wada  E Noga 《Journal of zoo and wildlife medicine》2000,31(2):259-261

Infection with Cladosporium sp., a deuteromycete, caused a deep dermal ulcer that extended to bone in a cultured tomato clownfish (Amphiprion frenatus). The infection may have been secondary to immunosuppression resulting from transport or confinement-induced stress or may have resulted from chronic exposure to copper. Reports of fungal infections in tropical marine fish are rare.  相似文献   

Synchronous climate changes in antarctica and the north atlantic     

EJ Steig  EJ Brook  JWC White  CM Sucher  ML Bender  SJ Lehman  DL Morse  ED Waddington  GD Clow 《Science (New York, N.Y.)》1998,282(5386):92-95

Central Greenland ice cores provide evidence of abrupt changes in climate over the past 100,000 years. Many of these changes have also been identified in sedimentary and geochemical signatures in deep-sea sediment cores from the North Atlantic, confirming the link between millennial-scale climate variability and ocean thermohaline circulation. It is shown here that two of the most prominent North Atlantic events-the rapid warming that marks the end of the last glacial period and the Bolling/Allerod-Younger Dryas oscillation-are also recorded in an ice core from Taylor Dome, in the western Ross Sea sector of Antarctica. This result contrasts with evidence from ice cores in other regions of Antarctica, which show an asynchronous response between the Northern and Southern Hemispheres.  相似文献   

Farm management factors associated with bulk tank somatic cell count in Irish dairy herds     

PT Kelly  K O'Sullivan  DP Berry  SJ More  WJ Meaney  EJ O'Callaghan  B O'Brien 《Irish veterinary journal》2009,62(Z4):S45

The relationship between bulk tank somatic cell count (SCC) and farm management and infrastructure was examined using data from 398 randomly selected, yet representative, Irish dairy farms where the basal diet is grazed grass. Median bulk tank SCC for the farms was 282,887 cells/ml ranging from 82,209 to 773,028 cells/ml. Two questionnaires were administered through face-to-face contact with each farmer. Herd-level factors associated with bulk tank SCC were determined using linear models with annual somatic cell score (i.e., arithmetic mean of the natural logarithm of bulk tank SCC) included as the dependent variable. All herd level factors were analysed individually in separate regression models, which included an adjustment for geographical location of the farm; a multiple regression model was subsequently developed. Management practices associated with low SCC included the use of dry cow therapy, participation in a milk recording scheme and the use of teat disinfection post-milking. There was an association between low SCC and an increased level of hygiene and frequency of cleaning of the holding yard, passageways and cubicles. Herd management factors associated with bulk tank SCC in Irish grazing herds are generally in agreement with most previous studies from confinement systems of milk production.  相似文献