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Jong-Tai Kim Jong-Yeol Park Hun-Su Seo Hwa-Gyun Oh Jae-Wuk Noh Jae-Hoon Kim Dae-Yong Kim Hee-Jeong Youn 《Veterinary parasitology》2002,103(1-2):53-63
Neospora caninum is an intracellular apicomplexan parasite that infects a wide range of mammals and has been associated with abortion in cattle worldwide. Artemisinin is an effective antimalarial compound derived from a traditional Chinese herbal remedy, qinghao or Artemisia annua L. In the study reported, the cultured host cells (vero cells or mouse peritoneal macrophages) infected with N. caninum tachyzoites were incubated with alpha-MEM (minimal essential medium) 10%HS supplemented with various concentration or artemisinin (20, 10, 1, 0.1 and 0.01 microg/ml) to examine the efficacy of artemisinin against N. caninum tachyzoites intracellular multiplication. In long-term studies, at 20 or 10 microg/ml for 11 days, artemisinin reduced N. caninum and completely eliminated all microscopic foci of N. caninum. At 1 microg/ml for 14 days, artemisinin reduced N. caninum and completely achieved elimination of all microscopic foci of N. caninum. There was no apparent toxicity to host cells in long-term studies. In short-term studies, at > or = 0.1microg/ml, artemisinin reduced N. caninum tachyzoites intracellular multiplication, significantly (P < 0.05) and appeared to depend on the artemisinin concentrations. Pretreatment of host cells or N. caninum tachyzoites with artemisinin had no effect on N. caninum tachyzoites intracellular multiplication. These results demonstrate that artemisinin inhibited N. caninum tachyzoites intracellular multiplication. 相似文献
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Effect of Restricted Feeding Regimes on Compensatory Growth and Body Composition of Red Sea Bream, Pagrus major 总被引:1,自引:0,他引:1
Sung-Yong Oh Choong Hwan Noh Sung Hwoan Cho 《Journal of the World Aquaculture Society》2007,38(3):443-449
Compensatory growth of red sea bream, Pagrus major, during feed deprivation and after refeeding was investigated. Groups of three fish each were allocated into 28 cages. Fish were fed by a commercial feed to satiation twice a day. Four feeding groups of fish were prepared: one group with continuous feeding (C) for 9 wk and three other groups with feed deprivation for 1 wk (F1) in Week 3, 2 wk (F2) from Week 2 to Week 3, and 3 wk (F3) from Week 1 to Week 3, respectively. All fish in the feed deprivation treatments resumed feeding in Week 4. The full compensatory growth was achieved in F1 and F2 fish after refeeding for the first 3 wk but in F3 fish after refeeding for the second 3 wk. Specific growth rate and feed conversion efficiency in all fish experiencing fasting were higher than those of control fish after first 3 wk of refeeding. At the end of feed deprivation in Week 3, crude protein, crude lipid, and energy content of all fish experiencing fasting were lower than those of the control fish. These results indicated that red sea bream experienced 1‐, 2‐, and 3‐wk fasting could achieve full compensatory growth in the 9‐wk feeding trial. 相似文献
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Kyoungsun Park Jaesang Ahn Sunmee Kang Euiri Lee Soohyun Kim Sangwan Park Sungwon Park Hyunwoo Noh Kangmoon Seo 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(4):557-561
The purpose of this study was to evaluate the effect of age on the ratio of pulp cavity/tooth width (P/T ratio) in healthy cats. The dental radiographs of 32 cats (16 males and 16 females) were generated with a digital dental X-ray unit with the animals under general anesthesia. Standardized measurement of the canine teeth was performed by drawing a line on the radiograph perpendicular to the cementoenamel junction (CEJ) of the tooth. There was an inversely proportional correlation between chronological age and the P/T ratio. Moreover, a strong Pearson squared correlation (γ2 = 0.92) was identified by the curved regression model. No significant differences in the P/T ratio based on gender or breed were found. These results suggest that determination of age by P/T ratio could be clinically useful for estimating the chronological age of cats. 相似文献
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Tae Kyung Yoon Saerom Han Dongho Lee Seung Hyun Han Nam Jin Noh Yowhan Son 《Journal of Forest Research》2014,19(2):249-259
Measuring coarse woody debris (CWD) respiration (R CWD) may have advantages over other approaches in determining CWD decomposition rates to accurately estimate forest carbon budgets and effects of warm temperatures on decomposition; however, studies on R CWD are insufficient. The R CWD from Quercus variabilis logs of different sizes (e.g., different surface area to weight ratios or weights) was measured under homogeneous conditions by using a closed-chamber system with a non-dispersive infrared sensor. The size effect on R CWD measured on a weight or volume basis was not significant, but it was significant on a surface area basis. This indicates that R CWD on a weight or volume basis would be a reliable measure, regardless of the size and cross-sectional area effects, while R CWD on a surface area basis must vary geometrically according to the change in sample size. R CWD did not change significantly over time until 122 h after sampling. An exponential model with a Q 10 of 2.34 was fitted only at temperatures below 22.6 °C because R CWD was suppressed at high temperatures due to constantly decreasing moisture. Instead, a logistic model was applied for all temperatures. The annual R CWD and the decay rate constant were estimated to be 53.4 g C kg?1 year?1 and 0.107 year?1, respectively. The decomposition rate estimate through R CWD might not correspond to that using the mass loss approach. It remains uncertain whether the methodological differences may lead to potential errors in measuring the actual CWD decomposition rate; therefore, a multiple approach study for CWD decomposition should be conducted. 相似文献
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Objective To describe the incidence rate of musculoskeletal (MS) injuries and investigate the factors that influence recovery from MS injury in young Thoroughbred horses in Australia. Methods Training and injury data were collected at fortnightly intervals from 14 trainers in New South Wales. Incidence rates were calculated for initial injuries stratified by age‐class and sex. Survival analysis methods were used to investigate the time to recovery after the first MS injury (measured as a start in a race or barrier trial). Results For the 248 racehorses enrolled, there were 428 injuries recorded; 2‐year‐olds were 2.99‐fold more likely than 3‐year‐olds to sustain an MS injury. The cumulative percentage of horses that had recovered within 6 months was 55%. The time to recovery was longest in horses whose fast gallop speed prior to injury was < 890 m/min. Conclusion This study supports the finding that shin soreness is the most common MS injury in young Australian Thoroughbreds. There was an increased rate of MS injury in 2‐year‐olds compared with 3‐year‐olds. However, it was impossible to determine if the increased risk in 2‐year‐olds was related to age, the start of training or a combination of both. The study also found that horses performing at higher exercise speeds prior to injury were more likely to recover. There was a significant effect of trainer, suggesting that other factors related to trainer may influence the time to the recovery. Additional research is required to understand the determinants of time to recovery. 相似文献
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Hyun-Kyoung Lee Byoung-Hee Lee Seung-Hyeok Seok Min-Won Baek Hui-Young Lee Dong-Jae Kim Yi-Rang Na Kyoung-Jin Noh Sung-Hoon Park Dutta Noton Kumar Hiroaki Kariwa Mina Nakauchi Suk-Jin Heo Jae-Hak Park 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(2):165-167
Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43. 相似文献