Equine pyoderma associated with malnutrition and unhygienic conditions due to neglect in a herd     

Inokuma H  Kanaya N  Fujii K  Anzai T  Maeda K  Okuda M  Onishi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(4):527-529

Twelve horses kept at a riding club suffered from pyoderma. All the horses displayed crusting, scaling and alopecia. The lesions were distributed in the chest, back, rump and limbs. Some of the horse patients also showed epilation with an attached crust similar to a 'paintbrush lesion' of dermatophilosis, but normal skin flora or opportunistic pathogenic bacteria were only isolated from the lesions. Some patients clearly showed weight loss, anemia and low levels of serum protein and cholesterol. General condition and skin lesions of the patients were improved gradually with improvement of feed and environment after being moved to new stables. Malnutrition under conditions of poor hygiene and poor management due to neglect might be associated with these equine cases of pyoderma in the herd.  相似文献   

Molecular cloning of the canine telomerase reverse transcriptase gene and its expression in neoplastic and non-neoplastic cells     

Yazawa M  Okuda M  Kanaya N  Hong SH  Takahashi T  Ohashi E  Nakagawa T  Nishimura R  Sasaki N  Masuda K  Ohno K  Tsujimoto H 《American journal of veterinary research》2003,64(11):1395-1400

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Detection of the anti-P53 antibodies in dogs with tumors     

Kanaya N  Okuda M  Toyama N  Oikawa T  Inokuma H  Morimoto M  Hayashi T  Une S  Nakaichi M  Taura Y  Tsujimoto H  Onishi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):973-979

To detect the anti-P53 antibodies of dogs with tumors, a GST-recombinant canine (rc) P53 fusion protein was expressed and purified. Immunoblot analysis was performed using this GST-rcP53 fusion protein as an antigen and serum samples from dogs suffering from tumors as primary antibodies. Out of 16 serum samples obtained from various tumor cases, four samples showed reaction with GST-rcP53. In contrast, serum from other 12 dogs with tumors, four dogs with non-neoplastic diseases and two control healthy dogs (as controls) did not show any reaction with GST-rcP53 in immunoblotting. The p53 gene mutation and the P53 protein expression were examined, using the tumor tissues to explore the relationship between the existence of the GST-rcP53 bands, gene mutations of p53 and the accumulation of P53 protein. One case, which showed a clear GST-rcP53 band, had a point mutation of the p53 cDNA and showed nuclear accumulation of P53 protein. These results suggest that the anti-P53 antibodies are also produced in tumor dogs with p53 gene mutations.  相似文献   

Molecular cloning and sequencing of feline CD7     

Ichimura N  Shimojima M  Nishimura Y  Tohya Y  Akashi H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(10):1255-1258

Human CD7 is one of the earliest molecules to appear in T cell development. In this study, putative feline CD7 cDNA was identified based on its similarities with human and mouse CD7 genes. The feline CD7 cDNA contained an open reading frame consisting of 630 nucleotides. The amino acid sequence of feline CD7 had 47.7% identity with that of human CD7, and 52.9% with that of mouse CD7. In addition, the feline CD7 protein fused with histidine tag was expressed in 293T cells. The expression was confirmed by indirect immunofluorescence assay.  相似文献   

A case of spontaneous Zymbal’s gland carcinoma with lung metastasis in an aged Fischer 344 rat     

Ai Maeno  Yoshimitsu Sakamoto  Motoki Hojo  Yukie Tada  Jin Suzuki  Akiko Inomata  Takako Moriyasu  Akihiko Hirose  Noriko Kemuriyama  Katsuhiro Miyajima  Dai Nakae 《Journal of toxicologic pathology》2021,34(4):353

Zymbal’s gland neoplasms are induced in rats through the administration of various carcinogens, but spontaneous neoplasia is rare. This report describes a spontaneous Zymbal’s gland carcinoma with lung metastasis found in an aged male Fischer 344 rat. Macroscopically, the dome-like tumor nodule, approximately 30 mm in diameter with ulceration, was located near the ear canal of the rat. No healthy tissue or structure of Zymbal’s gland was identified on the corresponding side, while the normal salivary glands and a lacrimal gland were observed. Histologically, a large part of the tumor mass was occupied by poorly differentiated neoplastic cells, the shapes of which were oval to polygonal or fusiform. Additionally, clusters of sebaceous-like foamy cells and squamous metaplasia with prominent keratinization were observed. Tumor cells were found to metastasize to the lung; these cells displayed histological similarities, including a sebaceous gland-like pattern, to those in the primary site. The tumor cells were immunohistochemically positive for cytokeratin AE1/AE3 or vimentin but negative for CD68, S100, α-smooth muscle actin, von Willebrand factor, and desmin. Our results indicate that the tumor was a poorly differentiated Zymbal’s gland carcinoma with lung metastasis.  相似文献   

Anesthetic Effect of a Combination of Medetomidine-Midazolam-Butorphanol in Cynomolgus Monkeys (Macaca fascicularis)     

Takehiro OCHI  Ippei NISHIURA  Mitsuyoshi TATSUMI  Yoshimi HIRANO  Kouichi YAHAGI  Yasuhiro SAKURAI  Kanako MATSUYAMA-FUJIWARA  Yuji SUDO  Noriko NISHINA  Hironari KOYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(6):917-921

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Fusion pore dynamics and insulin granule exocytosis in the pancreatic islet     

Takahashi N  Kishimoto T  Nemoto T  Kadowaki T  Kasai H 《Science (New York, N.Y.)》2002,297(5585):1349-1352

Insulin secretion from intact mouse pancreatic islets was investigated with two-photon excitation imaging. Insulin granule exocytosis occurred mainly toward the interstitial space, away from blood vessels. The fusion pore was unusually stable with a lifetime of 1.8 seconds. Opening of the 1.4-nanometer-diameter pore was preceded by unrestricted lateral diffusion of lipids along the inner wall of the pore, supporting the idea that this structure is composed of membrane lipids. When the pore dilated to 12 nanometers, the granules rapidly flattened and discharged their contents. Thus, our methodology reveals fusion pore dynamics in intact tissues at nanometer resolution.  相似文献   

Sequential extraction methods for soil organic nitrogen     

Rumiko KODASHIMA  Noriharu AE  Noriko KIKKOUJI  Shingo MATSUMOTO 《Soil Science and Plant Nutrition》2006,52(1):134-134

(pp. 825–831)
This study was carried out to clarify the effects of soil nitrate before cultivation and amounts of basal-dressed nitrogen on additional N application rate and yields of semi-forced tomato for three years from 1998 to 2000. The amounts and timing of additional N dressing were determined based on diagnosis of petiole sap nitrate. The top-dressing was carried out with a liquid fertilizer when the nitrate concentration of a leaflet's petiole sap of leaf beneath fruit which is 2–4 cm declined below 2000 mg L⁻¹.
For standard yield by the method of fertilizer application based on this condition, no basal-dressed nitrogen was required when soil nitrate before cultivation was 150 mg kg⁻¹ dry soil or higher in the 0–30 cm layer; 38 kg ha⁻¹ of basal-dressed nitrogen, which corresponds to 25% of the standard rate of fertilizer application of Chiba Prefecture, was optimum when soil nitrate before cultivation was 100150 mg kg⁻¹ dry soil; 75 kg ha⁻¹ of basal-dressed nitrogen, which corresponds to 50% of the standard, was optimum when soil nitrate before cultivation was under 100 mg kg⁻¹ dry soil. A standard yield was secured and the rate of nitrogen fertilizer application decreased by 49–76% of the standard by keeping the nitrate concentration of tomato petiole sap between 1000–2000 mg L⁻¹ from early harvest time to topping time under these conditions.  相似文献   

Isolation and identification of indigestible pyroglutamyl peptides in an enzymatic hydrolysate of wheat gluten prepared on an industrial scale     

Higaki-Sato N  Sato K  Esumi Y  Okumura T  Yoshikawa H  Tanaka-Kuwajima C  Kurata A  Kotaru M  Kawabata M  Nakamura Y  Ohtsuki K 《Journal of agricultural and food chemistry》2003,51(1):8-13

An enzymatic hydrolysate of wheat gluten was further digested in vitro with porcine pepsin and pancreatin to obtain an indigestible peptide. Indigestible pyroglutamyl peptide was isolated from the digest by strong cation-exchange, size-exclusion, and reversed-phase chromatographies. The pyroglutamyl peptide was digested with pyroglutamate aminopeptidase, and the digest was reacted with phenyl isothiocyanate. The resultant phenylthiocarbamyl (PTC) peptides were purified by reversed-phase HPLC by using binary gradient elution with ammonium acetate buffer, pH 6.0, and acetonitrile. The PTC peptides were analyzed with an automatic peptide sequencer on the basis of the Edman degradation method with a modified program. Some pyroglutamyl peptides were also analyzed by fast-atom bombardment ionization mass spectrometry without the pyroglutamate amino peptidase digestion. Consequently, pyroGlu-Asn-Pro-Gln, pyroGlu-Gln-Gln-Pro-Gln, pyroGlu-Gln-Pro-Gln, pyroGlu-Gln-Pro-Gly-Gln-Gly-Gln, pyroGlu-Gln, pyroGlu-Gln-Pro, pyroGlu-Ile-Pro-Gln, pyroGlu-Ile-Pro, pyroGlu-Gln-Pro-Leu, pyroGlu-Gln-Phe-Pro-Gln, pyroGlu-Ser-Phe-Pro-Gln, pyroGlu-Phe-Pro-Gln, and pyroGlu-Gln-Pro-Pro-Phe-Ser were identified.  相似文献   

Potential impacts of non‐native channel catfish on commercially important species in a Japanese lake,as inferred from long‐term monitoring data     

Shin‐Ichiro S. Matsuzaki  Noriko Takamura  Kazunori Arayama  Atsushi Tominaga  Jun Iwasaki  Izumi Washitani 《水产资源保护:海洋与淡水生态系统》2011,21(4):348-357