Two anonymous DNA segments distinguish the Wilms' tumor and aniridia loci   总被引：9，自引：0，他引：9  

L M Davis  R Stallard  G H Thomas  P Couillin  C Junien  N J Nowak  T B Shows 《Science (New York, N.Y.)》1988,241(4867):840-842

The association of Wilms' tumor with aniridia (the WAGR complex) in children with 11p13 chromosomal abnormalities has been established, but the paucity of molecular probes in 11p13 has hampered identification of the responsible genes. Two new anonymous DNA segments have been identified that map to the WAGR region of 11p13. Both DNA probes identify a cytologically undetectable deletion associated with a balanced chromosome translocation inherited by a patient with familial aniridia, but not Wilms' tumor. The same two DNA segments are also included in the distal p13-p14.1 deletion of another patient, who has aniridia, Wilms' tumor, and hypogonadism, but they are not included in the p12-p13 deletion of a third patient, who does not have aniridia but has had a Wilms' tumor. The discovery of this aniridia deletion and these two DNA segments that physically separate the Wilms' tumor and aniridia loci should facilitate identification of the genes in the WAGR locus, beginning with the aniridia gene.  相似文献   

Dynamics of arsenic-containing compounds sorption on sediments     

Reczynski  Witold  Posmyk  Grzegorz  Nowak  Katarzyna 《Journal of Soils and Sediments》2004,4(2):95-100

Background  River and lake sediments constitute complex and difficult analytical samples. On the other hand, sediments play a fundamental role in the distribution of toxic compounds in aquatic systems and in the evaluation of the current state and the course of changes taking place in the environment. Among elements present in the environment in trace concentrations, but having well-elaborated toxic properties, one of most dangerous is arsenic. The element occurs in the environment in several chemical forms, predominant are inorganic forms of As(V) and As(III), and methylated forms such as monomethylarsonic acid (MMAA) and dimethylarsinic acid (DMAA). Objectives  Reported herein are experiments, which were undertaken with the aim of examining the dynamics of arsenic sorption in sediments and its dependence on speciation of the element and the pH in the environment. Simultaneously, influence of organic matter content and chemical composition of the sediments on As sorption, were investigated. Methods  Sediment samples (upper 10 cm layer) were collected from three sites located in the vicinity of Cracow:

•	Rudawa river — 37 km long river, flowing mainly through the suburban area;
•	Vistula river — heavily contaminated, main Polish river;
•	Dobczyce reservoir — artificial reservoir on Raba river, total capacity of 125,000,000 m3, supplying about 60% of drinking water to the city of Cracow.

Using XRD and IR methods, mineral composition of sediments was analysed. Concentrations of iron, manganese, aluminium and arsenic as well as organic matter content in solid samples were analysed. Sorption experiment  100 g of dry sediment sample was placed in a 400 ml volume glass beaker. 300 ml of arsenic containing solution was poured into the beaker. Every 30 min (total experiment time span equalled 7 to 7.5 hours), 0.5 ml of the suspension’ (the beaker content was constantly mixed) was collected and centrifuged, and the concentration of arsenic was determined in the supernatant. In the series of experiments, solution contained about 0.05 μg/ml of arsenic in organic (DMAA) and inorganic forms, at different pH values — 3, 5, 7 and 9. Results and Discussion  Examined sediments of Vistula river and Dobczyce Lake were characterised by relatively high concentrations of arsenic, iron, manganese and aluminium.Rudawa river. At pH 3, the concentration of inorganic As in solution decreased from an initial value of 0.049 μg/ml to 0.012 μg/ml in 7.5 hours time. The same decreasing tendency was found at pH 5 (initial value 0.046 μg/ml, after 7.5 hours - 0.015 μg/ml). Less distinct sorption effects were noted at pH 7 and 9. Vistula river. At all pH values, a very significant decrease of inorganic As concentration up to 80% of the initial value, as well as a short time of achieving an equilibrium state (1 – 2.5 hours), was found. Dobczyce lake. Inorganic As was distinctly accumulated in sediments at pH 3 (decrease of As concentration in solution from 0.052 to 0.007 μg/ml after 7.5 hours of the experiment) and at pH 5. At pH 7, about 40% of As was accumulated in sediments (solution As concentration decrease from 0.050 to 0.030). Only 20% of As adsorbed on sediments at pH 9.Very weak accumulation of organic form of As (DMAA) was found in all examined sediments at pH 3 and 5. At higher pH values no sorption was registered. Conclusions  The following facts were established:

•	inorganic form of arsenic was effectively accumulated in all analysed sediments;
•	inorganic As sorption in sediments strongly depended on pH — for sediments of Rudawa river and Dobczyce lake, the highest sorption was found at pH 3, and a decrease of accumulated As was noted with an increase of pH (at pH 9, the lowest amount of As was immobilised in sediments); in the case of Vistula river sediments, a reverse tendency was found;
•	organic form of arsenic (DMAA) was accumulated only at pH 3 in all sediments and less significantly at pH 5 (Vistula river sediments); no sorption of that form of As was found in the other sediment samples;
•	state of equilibrium (no change in inorganic As concentration in liquid medium) was achieved in 1 hour for Vistula river sediments, after about 2 hours for Rudawa river and after about 4 hours for Dobczyce lake sediments.

Recommendations  The presented method of analysis of As sorption in sediments and results visualization (based on a newly defined coefficient) provides a useful tool for the characterization of sorptive properties of sediments and may be used for other compounds as well. Crucial, functional information — how much of a given element may be accumulated in sediments at a certain pH — is obtained without digestion of the sediment sample, in an experiment taking less than one day.  相似文献   

A low molecular weight immunosuppressive factor produced by Onchocerca gibsoni   总被引：2，自引：0，他引：2  

D Yin Foo  M Nowak  B Copeman  M Mc Cabe 《Veterinary immunology and immunopathology》1983,4(4):445-451

An immunosuppressive factor(s) which is heat stable, and dialysable, with a molecular weight less than 10,000 molecular weight, and is not species specific, has been shown to be produced during the in vitro culture of Onchocerca gibsoni microfilariae. The factor(s) is capable of abolishing lymphocyte mitogenesis induced by Con A in bovine lymphocytes and PHA in human lymphocytes. The factor(s) may play a role in establishing the microfilariae in the tissues of the host by depressing cell mediated immune reactions during invasion.  相似文献   

Cut-flower life of dry transported carnations as influenced by different silver form pre-treatments     

B. Le Masson  Joanna Nowak 《Scientia Horticulturae》1981,15(4):383-390

Twenty-four hours of pre-treatment with AgNO₃ increased the vase-life of flowers from 3 to 4 days for carnations kept in water or in preservative solutions after simulated transport conditions. A mixture of AgNO₃ with sodium thiosulphate or with EDTA was considerably better than AgNO₃, while such complexing agents as CDTA, EDDHA and EDPA were less effective. Determination of radioactive silver (Ag) in dissected carnation parts treated with different Ag complexes showed a positive correlation between vase-life and the amount of tracer Ag in the plants.  相似文献   

The physiology of hyacinth bulbs. XI. The effect of gibberellic acid on the growth and flowering of variously chilled bulbs     

M. Saniewski  J. Nowak  R.M. Rudnicki 《Scientia Horticulturae》1977,7(2):179-184

The effect of gibberellic acid, GA₃, on the growth and flowering of hyacinth cultivars ‘Pink Pearl’, ‘Delft Blue’ and ‘Carnegie’, chilled in a cold room at 5°C or a garden frame for 28, 42 or 81 days, and either rooted or dry, unrooted, was investigated. GA, was applied to the basal plates of the bulbs in a lanolin paste on 10 October 1975.The growth of the inflorescence and leaves of plants originating from dry-chilled bulbs was generally the same as that of those grown from rooted plants. A similar response to GA₃ treatment was observed in all cultivars. Treatment of bulbs with GA₃ decreased the number of days to flowering, stimulated the growth of inflorescences and leaves, and its effect was most pronounced in the plants chilled for shorter periods.  相似文献   

Immune responses of barramundi, Lates calcarifer (Bloch), after administration of an experimental Vibrio harveyi bacterin by intraperitoneal injection, anal intubation and immersion     

Crosbie PB  Nowak BF 《Journal of fish diseases》2004,27(11):623-632

Barramundi, Lates calcarifer (Bloch), were immunized with an experimental Vibrio harveyi bacterin via intraperitoneal injection, immersion and anal intubation. Both specific and non-specific immune parameters were measured to compare responses to bacterin after delivery by various methods. Elevated antibody activities in sera were found in all treatment groups with barramundi injected intraperitoneally displaying significantly higher antibody activity than the other groups. In addition, there was evidence of memory induction with a heightened antibody response in the intraperitoneally injected group only. Bacteriostatic assays indicated activity against V. harveyi in the sera of all bacterin-treated groups; again this activity was significantly higher in the intraperitoneally injected groups. There was no enhancement noted in head kidney macrophage phagocytic activity or in serum lysozyme levels.  相似文献   

Quantitation of immune response gene expression and cellular localisation of interleukin-1beta mRNA in Atlantic salmon, Salmo salar L., affected by amoebic gill disease (AGD)     

Bridle AR  Morrison RN  Cupit Cunningham PM  Nowak BF 《Veterinary immunology and immunopathology》2006,114(1-2):121-134

The characterisation of selected immune response genes during amoebic gill disease (AGD) in Atlantic salmon, Salmo salar L., was performed using semi-quantitative RT-PCR, quantitative real-time RT-PCR (qRT-PCR), and in situ hybridisation (ISH). The immune response genes of interest were interleukin-1beta (IL-1beta), inducible nitric oxide synthase (iNOS), serum amyloid A (SAA), and serum amyloid P-like pentraxin (SAP). Atlantic salmon were inoculated with the ectoparasite Neoparamoeba sp., the causative agent of AGD, and gill, liver and anterior kidney tissue sampled at 0, 7 and 14 d post-inoculation (p.i.). Semi-quantitative RT-PCR was performed on the tissue samples to identify up/down-regulated mRNA expression relative to uninfected control fish and normalised to the housekeeping gene, beta-actin. Interleukin-1beta (IL-1beta) was the only immune response gene of those investigated whose mRNA was differentially regulated in any of the tissues and was found to be up-regulated in the gills by semi-quantitative RT-PCR. Increased gill IL-1beta mRNA expression was then accurately quantitated and confirmed using probe-based qRT-PCR. The cellular localisation of the IL-1beta mRNA expression in the gills of uninfected and infected fish was then determined by ISH using an IL-1beta-specific biotinylated cRNA probe. Expression of IL-1beta mRNA was localised to filament and lamellar epithelium pavement cells in gills of uninfected and infected Atlantic salmon. These data implicate the involvement of IL-1beta at the site of infection, the gills, of Atlantic salmon during AGD. This work supports previous studies that suggest IL-1beta is important in the regulation of the fish immune response to parasitic infection but additionally shows the cellular localisation of fish IL-1beta mRNA expression during infection.  相似文献   

Validation of a method for the detection of virulent Yersinia enterocolitica and their distribution in slaughter pigs from conventional and alternative housing systems     

Nowak B  Mueffling TV  Caspari K  Hartung J 《Veterinary microbiology》2006,117(2-4):219-228

Various methods have been described in the literature for the detection of virulent Yersinia enterocolitica in pigs. The risk factors for pig herd contamination have yet to be determined. The objective of this study was to validate a sensitive method for the detection of Y. enterocolitica and to describe the distribution of the bacteria in pigs at slaughter from conventional and alternative ("organic") housing systems. First, samples were collected from tonsils, caecum with caecal contents, and the caecal lymph nodes of 60 slaughter pigs. These samples were used to compare the sensitivity of six different laboratory culture methods either in common use or described in the literature with that of a polymerase chain reaction with two primer pairs (multiplex PCR). Then, only PCR was used to examine tonsils, caecum and caecal lymph nodes from two groups of slaughter pigs: 210 from six conventional fattening farms and 200 from three with alternative housing. The results of the multiplex PCR were positive in 28 cases. All culture methods proved inferior to PCR in sensitivity. In the second part of the study, PCR detected 36 (18%) positive pigs from alternative housing and 60 (29%) from conventional housing (p<0.05). The highest rate of Y. enterocolitica contamination was found in tonsils (11% alternative, 22% conventional; p<0.05), followed by caecum (5%, 11%) and lymph nodes (2%, 7%). The housing system appears to be one important factor in the prevalence of this common pathogen in pig herds, as we found important differences between the two systems studied here. In the conventional system, the main risk factors appeared to be sourcing pigs from different pig suppliers, use of commercial feed and transportation to slaughter.  相似文献   

Effect of recovery day on DNA fragmentation in equine embryos     

M. BarańskaA. Okólski  A. Nowak 《Journal of Equine Veterinary Science》2012

  相似文献   

Antimicrobial susceptibility of Brachyspira hyodysenteriae isolated from 21 Polish farms     

Zmudzki J  Szczotka A  Nowak A  Strzelecka H  Grzesiak A  Pejsak Z 《Polish journal of veterinary sciences》2012,15(2):259-265

Swine dysentery (SD) is a common disease among pigs worldwide, which contributes to major production losses. Antimicrobial susceptibility testing of B. hyodysenteriae, the etiological agent of SD, is mainly performed by the agar dilution method. This method has certain limitations due to difficulties in interpretation of results. The aim of this study was the analysis of antimicrobial susceptibility of Brachyspira hyodysenteriae (B. hyodysenteriae) Polish field isolates by broth microdilution procedure. The study was performed on 21 isolates of B. hyodysenteriae, collected between January 2006 to December 2010 from cases of swine dysentery. VetMIC Brachyspira panels with antimicrobial agents (tiamulin, valnemulin, doxycycline, lincomycin, tylosin and ampicillin) were used for susceptibility testing of B. hyodysenteriae. The minimal inhibitory concentration (MIC) was determined by the broth dilution procedure. The lowest antimicrobial activity was demonstrated for tylosin and lincomycin, with inhibition of bacterial growth using concentrations > 128 microg/ml and 32 microg/ml, respectively. In the case of doxycycline, the MIC values were < or = 2.0 microg/ml. No decreased susceptibility to tiamulin was found among the Polish isolates and MIC values for this antibiotic did not exceed 1.0 microg/ml. The results of the present study confirmed that Polish B. hyodysenteriae isolates were susceptible to the main antibiotics (tiamulin and valnemulin) used in treatment of swine dysentery. Further studies are necessary to evaluate a possible slow decrease in susceptibility to tiamulin and valnemulin of B. hyodysenteriae strains in Poland.  相似文献