全文获取类型
收费全文 | 81774篇 |
免费 | 5137篇 |
国内免费 | 625篇 |
专业分类
林业 | 3134篇 |
农学 | 2489篇 |
基础科学 | 673篇 |
9646篇 | |
综合类 | 16374篇 |
农作物 | 3008篇 |
水产渔业 | 3727篇 |
畜牧兽医 | 42458篇 |
园艺 | 1121篇 |
植物保护 | 4906篇 |
出版年
2021年 | 712篇 |
2020年 | 793篇 |
2019年 | 964篇 |
2018年 | 1324篇 |
2017年 | 1547篇 |
2016年 | 1359篇 |
2015年 | 1301篇 |
2014年 | 1478篇 |
2013年 | 2878篇 |
2012年 | 2848篇 |
2011年 | 3441篇 |
2010年 | 2275篇 |
2009年 | 2161篇 |
2008年 | 3070篇 |
2007年 | 2948篇 |
2006年 | 2653篇 |
2005年 | 2572篇 |
2004年 | 2292篇 |
2003年 | 2349篇 |
2002年 | 2195篇 |
2001年 | 2478篇 |
2000年 | 2528篇 |
1999年 | 1938篇 |
1998年 | 789篇 |
1997年 | 702篇 |
1995年 | 740篇 |
1993年 | 664篇 |
1992年 | 1526篇 |
1991年 | 1716篇 |
1990年 | 1640篇 |
1989年 | 1588篇 |
1988年 | 1472篇 |
1987年 | 1526篇 |
1986年 | 1572篇 |
1985年 | 1396篇 |
1984年 | 1187篇 |
1983年 | 1015篇 |
1982年 | 679篇 |
1979年 | 1055篇 |
1978年 | 829篇 |
1977年 | 673篇 |
1976年 | 711篇 |
1975年 | 746篇 |
1974年 | 1003篇 |
1973年 | 984篇 |
1972年 | 951篇 |
1971年 | 878篇 |
1970年 | 851篇 |
1969年 | 804篇 |
1967年 | 701篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
111.
DEBORA J. OSUNA DVM Diplomate ACVS DAVID J. DEYOUNG DVM Diplomate ACVS RICHARD L. WALKER DVM PhD MPVM 《Veterinary surgery : VS》1992,21(6):458-462
The antimicrobial efficacy of an adhesive drape applied after a 1-minute alcohol scrub was compared to a povidone-iodine (PI) skin preparation technique in dogs. Each technique was applied to both sides of 15 adult anesthetized dogs on premeasured, clipped areas of skin. Skin bacteria were quantified before, immediately after, and 1 hour after skin preparation. Predominant skin bacteria were isolated by swabbing the skin. The percentages of bacterial reduction immediately after and 1 hour after skin preparation, percentages of negative culture results, cultures with more than five colony-forming units, and the frequency of skin reactions were calculated and analyzed statistically. Drape adhesion was assessed subjectively. The percentage reduction in skin bacteria was significant for both techniques and comparable to that reported in humans. The adhesive drape was significantly less effective in both the immediate and 1-hour periods. Lift occurred in 66% of drape applications but was not associated with high bacterial counts. Acute contact dermatitis was more frequent after skin preparation with PI. There was no difference between the techniques in recovery of potential skin pathogens. The authors conclude that application of this antimicrobial adhesive drape after a 1-minute alcohol scrub is not as effective in the reduction of skin bacteria in dogs as is PI preparation of the skin. 相似文献
112.
Evaluation of the long-acting somatostatin analogue Octreotide in the management of insulinoma in three dogs 总被引:1,自引:0,他引:1
K. W. Simpson R. L. Stepien C. M. Elwood A. Boswood C. R. Vaillant 《The Journal of small animal practice》1995,36(4):161-165
The response of dogs with insulinoma to surgical and medical management is variable, with the majority developing intractable hypoglycaemia. A long-acting somatostatin analogue, Octreotide (SMS 201–995; Sandostatin) has been useful in the management of hypoglycaemia in humans with insulinoma, and preliminary reports suggest a beneficial clinical response in dogs with insulinoma. The present study objectively evaluated Octreotide in the management of three dogs with immunohistochemically confirmed insulinoma. Octreotide had no benefit over placebo, and little effect on circulating glucose and insulin concentrations despite clearly detectable plasma concentrations of Octreotide. No clinical improvement was apparent in two dogs given Octreotide over a period of two and three weeks. These results contrast with the positive clinical responses noted previously and indicate that further placebo controlled, objective studies are necessary before clear statements on the treatment of insulinoma with Octreotide are made. 相似文献
113.
M L van der Leek J B Dame C L Adams K D Gillis R C Littell 《American journal of veterinary research》1992,53(6):877-882
Experimental and field trials were conducted to evaluate an ELISA for its ability to detect Trichinella-infected domestic swine and to compare ELISA results with muscle-digestion test results. The ELISA used was a commercial double-antibody kit, containing an excretory-secretory antigen, and was evaluated principally for epidemiologic use. Experimentally induced infection in swine (4 groups of 3 pigs each; inoculated with 0, 50, 500 or 5,000 larvae) was detected as early as postinoculation week 4, with seroconversion of all inoculated swine by postinoculation week 8. The rate of seroconversion appeared to be affected by initial larval dose, time after inoculation, and immunocompetence of the individual host. Determination of antibody kinetics generally revealed rapidly increasing antibody titer, followed by its steady decrease in most pigs. Once seropositive, however, all pigs remained seropositive for the duration of the 10-week study. Presence of muscle larvae was confirmed in all infected pigs at termination of the study. We recognize that the experimental conditions may not be truly representative of those under which natural infection develops in pigs; however, the ELISA detected an infected pig with muscle larval density of 0.87 larvae/g of tissue. Results of a field trial (n = 310) indicated no muscle digestion test-positive pigs (35 g of diaphragm muscle digested/pig), but 3 samples tested positive by ELISA for specificity of 99.0%. 相似文献
114.
Lyophilised serum offers significant advantages over frozen serum when it comes to shipping such material over long distances. Babesia bigemina and B bovis were cultured in medium supplemented by either frozen-thawed or lyophilised-rehydrated serum. There were no significant differences between the two types of medium in the growth of parasites and percentage of infected cells during subcultivation for 18 days. 相似文献
115.
116.
117.
Four procedures were compared for isolation of Staphylococcus aureus from swabbing solutions of teat skin and milking unit liners from commercial dairies. In 2 procedures, 0.1 ml of swabbing solutions were added to either 5 ml Vogel-Johnson or Baird Parker broth media and enriched at 37 degrees C, 4 h. Following enrichment, 0.1 ml culture was transferred to modified Baird-Parker agar and incubated at 37 degrees C, 48 h. In the other 2 procedures, 0.1 ml of swabbing solution was directly placed on either blood or modified Baird-Parker agar plates and incubated at 37 degrees C 48 h. Combining results from all methods, Staphylococcus aureus were isolated from 72 of 913 (7.9%) skin samples, and 34 of 268 liners (12.6%). On average, 43.1% (31/72) of the S. aureus isolates were found by the enrichment in liquid Vogel-Johnson procedure. The average isolation percentage for other methods ranged from 19.4% to 25.0%. Isolation of S. aureus from milking unit liner or teat skin swabbing solutions was approximately twice as likely after enrichment in Vogel-Johnson liquid media as opposed to other methods of isolation. This indicates that enrichment in Vogel-Johnson liquid media improved recovery of S. aureus from swabbing solutions. 相似文献
118.
119.
Effect of dietary protein content on episodic growth hormone secretion and on heat production of male broiler chickens. 总被引:1,自引:0,他引:1
1. The effect of the crude protein content (200 and 150 g/kg) of isoenergetic diets on episodic growth hormone (GH) release and on heat production was investigated in male broiler chickens. 2. Decreasing the crude protein content of isoenergetic diets from 200 g/kg (HP diet) to 150 g/kg (LP diet) resulted in depressed body weight gain, impaired food conversion efficiency and increased abdominal fat deposition. 3. The pattern of growth hormone secretion was markedly affected by dietary treatment. Broiler chickens fed on the LP diet had higher overall mean, amplitude, baseline and peak frequency than the HP chickens. 4. The LP chickens produced more heat per unit of metabolic body weight than the HP chickens. 5. The hypothesis relating the pattern of GH secretion to protein conversion efficiency was corroborated. 相似文献
120.
A Fernandez J Perez L Carrasco M J Bautista J M Sanchez-Vizcaino M A Sierra 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1992,39(6):393-402
Lymph nodes, spleen, liver, lung and kidney obtained from pigs experimentally infected with two African Swine Fever Virus (ASFV) isolates of differing virulence were fixed by perfusion with glutaraldehyde and embedded in paraffin. An immunoperoxidase technique using a polyclonal anti-ASFV serum was performed on tissue sections in order to detect ASFV antigen. The distribution of ASFV antigen in such infected organs is shown and the differences between both infections compared and discussed. Monocytes, macrophages, hepatocytes, endothelial cells, neutrophils and epithelial cells were found to contain ASFV antigens. 相似文献