Assessment of changes in hemostatic markers in Cavalier King Charles Spaniels with myxomatous mitral valve disease     

Tarnow I  Kristensen AT  Olsen LH  Pedersen HD 《American journal of veterinary research》2004,65(12):1644-1652

OBJECTIVE: To evaluate markers of hemostasis and their relationship to the degree of mitral regurgitation (MR) and platelet function in Cavalier King Charles Spaniels (CKCSs) with myxomatous mitral valve disease. ANIMALS: 76 clinically healthy CKCSs and 24 control dogs. PROCEDURE: All dogs underwent echocardiographic examination; various hemostatic, hematologic, and biochemical variables were evaluated in blood. The CKCSs were allocated to 1 of 3 groups on the basis of MR severity. In 8 control dogs and 8 CKCSs, plasma von Willebrand factor (vWF) multimer analysis was performed. RESULTS: Compared with control dogs, plasma fibrinogen concentration was higher in all CKCSs and related to left ventricular end diastolic diameter and left atrial-to-aortic root ratio among all CKCSs. The activated partial thromboplastin times and plasma D-dimer concentration were similar among the 4 groups. Plasma vWF concentration was lower in CKCSs with moderate to severe MR, compared with that of CKCSs with no MR and control dogs. There was a relationship between plasma vWF concentration and platelet function in CKCSs but not in control dogs. In 4 CKCSs with moderate to severe MR and low plasma vWF concentration, amounts of vWF high-molecular-weight multimers (HMWMs) were low. CONCLUSIONS AND CLINICAL RELEVANCE: In CKCSs, MR appeared to be associated with a low plasma vWF concentration and likely a loss of vWF HMWMs (possibly through their destruction via shear stress to the blood). The importance of the changes in plasma fibrinogen concentration and the thromboembolic risk in dogs with MR remain to be investigated.  相似文献   

Determination of true digestive utilization of phosphorus and the endogenous phosphorus outputs associated with soybean meal for growing pigs   总被引：9，自引：0，他引：9  

Ajakaiye A  Fan MZ  Archbold T  Hacker RR  Forsberg CW  Phillips JP 《Journal of animal science》2003,81(11):2766-2775

The objectives of this study were to determine true P digestibility, the gastrointestinal endogenous P outputs associated with soybean meal (SBM), and the role of the large intestine in P digestion in growing pigs. Four Yorkshire barrows, with average initial and final BW of 40 and 58 kg, were fitted with a simple T-cannula at the distal ileum and fed four diets according to a 4 x 4 Latin square design. The diets were cornstarch-based and contained four levels of P (0.098, 0.196, 0.293, and 0.391% on a DM basis) from solvent-extracted conventional SBM. Chromic oxide (3.5 g/kg of diet, as-fed basis) was included as a digestibility marker. Each experimental period consisted of 8 d with a 4-d adaptation period and a 4-d collection of representative ileal digesta (2 d) and fecal (2 d) samples. True ileal and fecal P digestibility values and the ileal and fecal endogenous P outputs associated with SBM were determined by the regression analysis technique. There were no differences (P > 0.05) in true P digestibility values (ileal, 59.0 +/- 8.3 vs. fecal, 51.3 +/- 7.9%, n = 16) and endogenous P outputs (ileal, 0.59 +/- 0.18 vs. fecal, 0.45 +/- 0.21 g/kg of DMI, n = 16) between the ileal and the fecal levels. The endogenous fecal P loss accounted for 8.1 and 17.6% of the NRC (1998) recommended total and available P requirements in growing pigs, respectively. In conclusion, approximately 51% of the total P in conventional SBM is digested in growing pigs. The large intestine does not play an important role in the digestion of P associated with SBM in the growing pig. The fecal loss of the gastrointestinal endogenous P is an important route of P excretion in the growing pig.  相似文献   

Residents’ use of remnant natural vegetation in the residential area of Järvafältet, Stockholm     

Clas Florgrd  Oskar Forsberg 《Urban Forestry & Urban Greening》2006,5(2):1

In growing cities, there is a demand for development areas, which poses a threat to existing green areas. Therefore, it is of interest to know to what extent different types of green areas are used by residents. In the suburb of Järvafältet, situated 15 km outside Stockholm city centre, inhabitants’ use of patches of remnants of original natural vegetation in green areas has been studied by momentary observations from fixed points over the periods 1978–1982 and 2002–2004. The number of people, sex, age, type of activity, and place of that activity were noted. Overall, there has been a significant increase in the amount of use of these types of recreation areas between the two periods. The use by organized groups, as well as by adult walkers, runners and cyclists, increased the most, while individual children's play decreased. Almost every other child observed was found to be partaking in organized school or day nursery group activities. Children belonging to organized groups were seen as frequently in areas at a distance of less than 100 m from the houses as between 200 and 400 m from the built-up area, while children not participating in group activities were seen much more frequently in the areas closest to buildings. These types of green areas were used more by children and young people than by adults. This implies the likelihood that the use of these areas for recreation is underestimated in outdoor recreation inquiries and interview studies, where children are not usually included. Residents’ use of areas with remnant natural vegetation in cities shows that these areas have a recreational value as well as with other values. This makes them important to take into consideration in the planning and design process. Small areas close to residential areas need to be considered.  相似文献   

Assessment of Molasses–Urea Blocks for Goat and Sheep Production in the Sultanate of Oman: Intake and Growth Studies     

Forsberg NE  al-Maqbaly R  al-Halhali A  Ritchie A  Srikandakumar A 《Tropical animal health and production》2002,34(3):231-239

The goal of this study was to develop a molasses–urea block (MUB) for purposes of supplementing trace minerals to domestic ruminant livestock in Oman. To accomplish this, the utility of molasses and date syrup as fermentable energy sources, of straw, date flakes and wheat bran as fibre sources, and of cement and lime as binders were evaluated. The proportion of cement needed for adequate hardening of the block was also studied. Molasses- and date syrup-based blocks hardened equally well. However, the higher cost of date syrup precluded its use. Wheat straw yielded a low-density block that hardened slowly. Date fibre retained moisture and hardened extremely slowly. Wheat bran-based blocks hardened quickly and yielded dense blocks. Hence, wheat bran was judged to be the superior source of fibre. Lime did not effectively bind the blocks. A cement content of 15% allowed hardening of the blocks within 2–3 weeks. A level of 10% cement in the block reduced the hardening rate by about 50%. Sheep and goats consumed both the straw- and wheat bran-based blocks but at different rates. Consumption of the straw-based block by sheep ranged from 50 to 179 g/head per day, whereas the denser wheat bran-based block was consumed at a rate of 8–20 g/head per day. Consumption of the straw-based block by goats was low (8 g/head per day) compared to that of wheat bran-based blocks (16–24 g/head per day). On the basis of the intake of the bran-based block by sheep, a block was designed that would provide approximately 50% of an animal's trace mineral requirements per day. This block consisted of 45% molasses, 10% urea, 5% trace minerals, 2.5% NaCl, 22.5% wheat bran and 15% cement. Sheep consuming this block gained more weight than sheep fed a conventional mineral block or sheep receiving no mineral supplementation. MUBs are inexpensive (9.5 US cents/kg). We conclude that MUBs have utility for providing trace elements in ruminant diets.  相似文献   

Identification of Toxoplasma gondii and Encephalitozoon cuniculi by immunoperoxidase techniques and electron microscopy, in stored, formalin-fixed, paraffin-embedded tissue     

Inge Bjerks  Thor Landsverk 《Acta veterinaria Scandinavica》1986,27(1):11-22

The peroxidase-anti-peroxidase (PAP), avidin-biotin-complex (ABC) techniques, and electron microscopy, were used to identify protozoan parasites in formalin-fixed material from routine necropsy cases. The material comprised paraffin blocks, that had been stored for up to 18 years, from 18 cases of suspected toxoplasmosis and encephalitozoonosis. With the ABC method a higher dilution of primary antibody could be used than with the PAP method. However, with both methods, a distinct reaction occurred with appropriate dilutions. The age of the material did not seem to effect the result. Frequently, phagocytized and necrotic parasites were also stained. Gystozoites (bradyzoites) of T. gondii were stained faintly compared to the endozoites (tachyzoites). A Toxoplasma-like parasite from a dog did not react with anti-Toxoplasma serum, and ultrastructurally it proved to be consistent with an unidentified cyst-forming sporozoan parasite previously reported in dogs. Electron microscopy based on paraffin-embedded tissue, seems to be a valuable method for identification of protozoan parasites, and thus provide a supplement or alternative to the immunoperoxidase methods.  相似文献   

Growth of pot roses and post-harvest rate of water loss as affected by air humidity and temperature variations during growth under continuous light     

Rolf Inge Pettersen  Roar MoeHans Ragnar Gislerød 《Scientia Horticulturae》2007

The effect of diurnal variations in air humidity and temperature under continuous lighting period (LP) on growth, flowering and water loss were studied in two pot-rose cultivars.  相似文献   

Pathological Conditions of the Reproductive Organs of Male Stray Dogs in the Tropics: Prevalence, Risk Factors, Morphological Findings and Testosterone Concentrations     

A Ortega-Pacheco  JC Rodríguez-Buenfil  JC Segura-Correa  ME Bolio-Gonzalez  M Jiménez-Coello  C Linde Forsberg 《Reproduction in domestic animals》2006,41(5):429-437

The objective of this study was to estimate the prevalence of and risk factors for pathological conditions of the reproductive organs in stray dogs under tropical conditions. Three hundred and eighteen dogs were examined post-mortem in the period from 1 July 2002 to 30 June 2003. Before killing, a blood sample (from the cephalic vein) for testosterone assay was taken. Pathological conditions of the reproductive organs were found in 135 of the dogs (42.5%) and in 175 of the testes (64.8%). The most frequent pathologies found were testicular degeneration, cryptorchidism, testicular hypoplasia and testicular tumours (in 15.1%, 6.6%, 6.6% and 5.4% of the dogs and 15.1, 4.6, 6.0 and 3.5 of the testes, respectively). Transmissible venereal tumour (TVT) was seen in 5.4% of the dogs. Testicular degeneration was more common in old dogs and underweight dogs (p < 0.05). Testicular tumours were 14.3 times more common in cryptorchid dogs. Age was another important factor for the development of testicular tumours (p < 0.05). Lower levels of testosterone concentration (p < 0.05) were observed in dogs with advanced testicular degeneration (0.7 +/- 0.8 nM), dogs with hypoplastic testicles (0.8 +/- 0.9 nM) and dogs with one degenerated and one retained testis or with bilateral cryptorchidism (1.2 +/- 0.9 nM) compared to dogs with one or two normal testes (7.0 +/- 5.5 nM). Testicular volume and weight were significantly lower in degenerated, hypoplastic and retained testes compared with the contralateral normal testis. Some spermatogenic activity was found in three of the retained testes, producing oligozoospermic smears with a high percentage of sperm abnormalities. No comparable epidemiological data about male pathological conditions of the reproductive organs in the dog is available. The prevalence found in this study, yet, appears high.  相似文献   

Influence of stress and nutrition on cattle immunity.   总被引：1，自引：0，他引：1  

Jeffery A Carroll  Neil E Forsberg 《Veterinary Clinics of North America: Food Animal Practice》2007,23(1):105-149

Today, the scientific community readily embraces the fact that stress and nutrition impact every physiologic process in the body. At last, the specific mechanisms by which stress and nutrition affect the immune function are being elucidated. The debate among animal scientists concerning the definition and quantification of stress as it relates to animal productivity and well-being is ongoing. However, an increased appreciation and understanding of the effects of stress on livestock production has emerged throughout the scientific community and with livestock producers. The intent of this article is to provide an overview of the general concepts of stress and immunology, and to review the effects of stress and nutrition on the immune system of cattle.  相似文献   

The Effects of Replacing Rhodes Grass (Chloris gayana) Hay with Ghaf (Prosopis cineraria) Pods on the Performance of Omani Native Sheep     

Mahgoub O  Kadim IT  Al-Ajmi DS  Al-Saqry NM  Al-Abri AS  Richie AR  Annamalai K  Forsberg NE 《Tropical animal health and production》2004,36(3):281-294

A feeding trial using various levels of dry pods of ghaf (Prosopis cineraria) was carried out with 28 Omani native male sheep. Individual feed intake and body weight were determined for 50 days to allow assessment of the effects of the feeds on growth, feed intake, feed conversion and carcase measurements. The ripened ghaf pods contained 91% dry matter (DM), 13.5% crude protein (CP), 14.3% crude fibre (CF), 1.3% ether extract (EE) and 5.2% ash (on DM basis). Four levels of ghaf (0, 15%, 30% and 45%) were used with corresponding decreasing proportions of Rhodes grass (RGH) hay. Animals fed rations containing RGH as the sole source of roughage and no ghaf grew faster (135 g/day) and had better feed conversion than those fed various levels of ghaf. Sheep fed 15% ghaf gained reasonably well (90 g/day) and had carcase attributes similar to those fed no ghaf. Sheep fed 30% ghaf still gained weight but those fed 45% ghaf lost weight. Negative effects on growth appeared to occur after 6 weeks of feeding ghaf.  相似文献   

Biological Seed Treatment of Cereals with Fresh and Long-term Stored Formulations of Clonostachys rosea: Biocontrol Efficacy Against Fusarium culmorum     

Birgit Jensen  Inge M.B. Knudsen  Dan Funck Jensen 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(3):233-242

In six field experiments, seed treatment with Clonostachys rosea (IK726) significantly reduced disease caused by Fusarium culmorum. IK726 was active against the pathogen at average soil temperatures at sowing ranging from 6.2 to 12 °C. Both in the field experiments and in growth chamber experiments conducted in sand, dried and stored conidia of IK726 controlled F. culmorum as effectively as freshly harvested conidia. A high correlation was found between disease index ratings from field experiments and from corresponding growth chamber sand tests. Amendment with the stickers Pelgel or Sepiret did not influence control activity. The effective dosages of IK726 (cfu/seed) were estimated in bioassays and were very similar for freshly harvested conidia and for dried conidia. With a density of > 5×10³ conidia per seed more than 80% disease control was repeatedly obtained with both types of conidia.  相似文献