猪用加味玉屏风缓释微丸水提醇沉工艺研究     

梁运霞  尚磊  刘伟  梁晓庆  孟妍  庞淑华  梁立 《中兽医医药杂志》2015,(1):42-44

目的:考察猪用加味玉屏风缓释微丸的水提醇沉工艺。方法:以黄芪甲苷的含量及干膏得率作为评价指标,选用L9(34)进行正交试验。结果:猪用加味玉屏风缓释微丸最佳水提取工艺为药材分别加12倍量水,提取2次,提取时间分别为0.5 h;最佳醇沉工艺为浓缩药液相对密度1.15,加醇浓度70%,静置时间为12 h。结论:优选出的水提醇沉工艺科学、合理。  相似文献   

基于VBA的云南省基层林业工作站信息管理系统的设计与构建     

钱发斌  艾建林  杨建祥  邓喜庆  年顺龙 《林业调查规划》2014,39(4):80-83

为全面掌握全省各级林业站管理机构、各县(市、区)基层林业工作站的基本情况,基于云南省基层林业工作站情况调查的要求,结合乡镇林业工作站工程建设标准需要,以林业工作站调查信息数据为基础,利用VBA语言在Access 2007下开发具有形象直观的统计图功能、智能查询功能、数据导出至电子表格模板等功能特点的云南省基层林业工作站信息管理系统,文中阐述了系统开发方案、开发环境及系统主要功能。  相似文献   

奶牛TNMD基因的克隆与原核表达     

庞坤  肖世文  陈宏智  易本驰  刘纪成  韩立强 《湖北农业科学》2014,(7):1681-1683

从奶牛组织中克隆Tenomodulin(TNMD)基因的cDNA序列,采用双酶切后连接表达载体pGEX-4T-1,转入大肠杆菌BL21中进行诱导表达。结果表明,TNMD基因的序列全长为957 bp,通过双酶切构建的表达载体pGEX-TNMD在BL21大肠杆菌中成功表达了分子量为59.68 kDa的融合蛋白。  相似文献   

Biological functions and clinical significance of secretogranin III     

LI Feng-rui  TIAN Xiao-fei  ZHOU Yi-shu  ZHU Lan-hui  PANG Hao 《园艺学报》2011,27(9):1839-1843

AIM: Secretogranin III (SCG3/Sg III) is extensively distributed in endocrine or endocrine-like cells in human body and plays an important role in some biological functions by regulating peptide hormone secretion and neuroendocrine activity.The distribution and functions of SCG3 make itself as not only one of the most useful biological markers in association with neuroendocrine functions, but also a potential indicator of diagnostic and prognostic evaluation in certain diseases.The gene and protein structures, tissue expression, biological functions as well as pathophysiology and clinical significance of SCG3 are reviewed in this article.  相似文献   

白菜型春油菜新品种陇油18号选育报告   总被引：3，自引：1，他引：2  

刘婷婷  闫春梅  徐一涌  齐燕妮  庞进平 《甘肃农业科技》2019,(9):1-4

白菜型春油菜新品种陇油18号是甘肃省农业科学院作物研究所油菜课题组以门源油菜为母本、E144为父本杂交选育而成的极早熟白菜型春油菜常规种。2014—2015年参加甘肃省极早熟组春油菜区域试验,陇油18号2 a 10点次平均折合产量为2 130.15 kg/hm2,较对照品种甘南4号增产16.40%,增产油籽300.15 kg/hm2。陇油18号生育期108 d,平均株高126 cm,一次有效分枝数3～6个,单株果数268个,角粒数20.85粒,千粒重2.98 g。籽粒含油量(粗脂肪)38.89%,芥酸含量0.98%,硫苷含量22.41μmol/g,品质达到国家"双低"标准。适宜在甘肃张掖、武威、甘南、定西等海拔2 400 m以上春油菜种植区种植。  相似文献   

Effect of One-off Application of Poly Urea-Formaldehyde Fertilizer Under Reduced N Rate on Double Cropping Rice   总被引：2，自引：0，他引：2  

QiaoYi HUANG  Mu ZHANG  Xu HUANG  ShuanHu TANG  FaBao ZHANG  YuWan PANG  Qiong YI  Ping LI  HongTing FU 《中国农业科学》2018,51(20):3996-4006

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Effect Comparison of Adenovirus Vector Transfer into Different Buffalo Cells     

DUAN An-qin  PANG Chun-ying  ZHU Peng  LU Xing-rong  DENG Ting-xian  LIANG Xian-wei 《中国畜牧兽医》2016,43(10):2661-2665

Buffalo mammary epithelial cell,cumulus cell and fibroblasts were transfected by adenovirus vectors and compared their transfection efficiency.293 cells were transfected with pBHGloxdelE13cre and pDC316-eGFP by liposome,the virus was collected and titer was detected.Buffalo mammary epithelial cell,cumulus cell and fibroblasts were exposed to different multiplicity of infection (MOI) of adenovirus vectors.After 72 h,the cells were observed with inverted fluorescence microscope,and transfection efficiency was calculated.When the MOI was 25,50,100,200 and 400,the transfection efficiency of fibroblasts were 0.7%,7.0%,9.0%,12.5% and 34.0%,the transfection efficiency of cumulus cells were 42.5%,55.3%,57.4%,76.0% and 80.0%,the transfection efficiency of mammary epithelial cells were 88.7%,100%,100%,100% and 100%.The results showed that the transfection efficiency of mammary epithelial cell was the best,followed by cumulus cell,and fibroblast was poor.  相似文献   

芒果采后炭疽菌Colletotrichum asianum鉴定及生物学特性研究     

孙珊  谭丽玲  庞小婧  吴文嫱  黄东益  黄小龙 《热带作物学报》2016,37(12):2392-2397

采用组织块分离法从采后发病芒果果肉中分离病原菌,通过形态学观察、r DNA-ITS序列和系统发育树分析对其进行鉴定,并初步研究其生物学特性。结果表明:从发病芒果中分离到的病菌T0408鉴定为Colletotrichum asianum Prihastuti,L.CaiK.D.Hyde。该菌菌丝生长的适宜温度为20~30℃、适宜pH值为5~8;环境湿度在75%~98%范围内,湿度越大,菌丝生长速度越快,在3 000 lx日光灯的光照强度下连续光照有利于菌丝的生长和产孢;孢子萌发的适宜温度为25~30℃、适宜pH值为6~8,环境湿度为75%~98%,湿度越大、在3 000 lx日光灯的光照强度下连续光照有利于孢子的萌发。  相似文献   

鹌鹑ESR1基因的外显子8多态性与早期生长性能的关联分析     

樊红灯  白俊艳  付学言  曹恒  杨帅  庞有志  时坤鹏  卢小宁  马永康  陈梦柯  董智豪  安肖凯  常世增  侯伟晓  巩慧荣 《黑龙江畜牧兽医》2020,(2)

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水源地生态环境综合治理方案研究     

张家荣  刘建林 《安徽农业科学》2016,(11)

在分析水源地生态环境综合治理重要性的基础上,从把握水源地现状,分析水源地环境问题,合理划分保护区,确定治理规划内容及规模等方面,提出了水源地生态环境综合治理的步骤,并建立了综合治理方案。  相似文献