中国发现光瘤姬蜂(膜翅目:姬蜂科)     

裴海潮 《中国森林病虫》2007,26(1):23-24

报道采自河南陕县的姬蜂科中国1新记录属、新记录种——毛光瘤姬蜂Liotryphon crassiseta（Thomson，1877）。介绍了该属的主要鉴别特征和该种的形态特征、寄主、分布。  相似文献   

烘烤工艺对烟叶色素及主要化学成分的影响     

裴晓东  刘涛  刘卉  王建波 《作物研究》2012,26(Z2)

以烤烟G80为材料,研究了不同烘烤工艺对叶片色素含量及主要化学成分的影响.结果表明:中温中湿烘烤工艺有利于烟叶内部物质的充分转化,促进上等优质烟叶的形成.  相似文献   

Effects of STK15 overexpression on growth of human esophageal carcinoma cell line KYSE150     

WANG Xiao-xia  LI Xiao-zhong  LU Na  ZHAO Yan  WANG Kang  PEI Yi 《园艺学报》2013,29(11):1957-1961

AIM:To investigate the effects of serine/threonine kinase 15 (STK15) overexpression on the growth of human esophageal squamous-cell carcinoma (ESCC) cell line KYSE150. METHODS:Recombinant pEGFP-C1-STK15 expression vector was transfected into KYSE150 cells using LipofectamineTM 2000 and the expression of STK15 was detected by fluorescence microscopy and Western blotting. The proliferation of the cells in vitro was measured by MTT assay. The cell cycle distribution and apoptosis were detected by flow cytometry. The proliferation of the cells in vivo was measured by tumorigenicity experiment in nude mice. RESULTS:After recombinant pEGFP-C1-STK15 expression vector was stably transfected into KYSE150 cells, GFP-STK15 fusion protein localized to centrosome and spindle. The STK15-overexpressing colonies were further confirmed by Western blotting. MTT assay showed that the proliferation of the cells in STK15 overexpression group was increased compared with control group (P<0.01). Flow cytometry analysis showed that the percentage of the cells in G0/G1 phase and the cell apoptosis in STK15 overexpression group were decreased compared with control group (P<0.01). The tumorigenicity experiment in nude mice showed that the proliferation of the cells in STK15 overexpression group was increased compared with control group (P<0.01). CONCLUSION: Overexpression of STK15 in human ESCC KYSE150 cells promotes the cell growth in vitro and in vivo, indicating that STK15 may serve as a novel therapeutic target for esophageal carcinoma.  相似文献   

Host response to rickettsial infection in blue mussel, Mytilus edulis L.     

G. GULKA  PEI WEN CHANG 《Journal of fish diseases》1985,8(3):319-323

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棉花铃重AFLP标记的初步研究   总被引：2，自引：1，他引：1  

方卫国  张正圣  李先碧  裴炎 《棉花学报》2000,12(4):176-179

本文以陆地棉铃重近等基因系为材料(大铃≥5.4g,小铃≤1.7g),构建大小铃两个近等基因池.利用AFLP标记技术对两基因池进行分析,64对引物组合共产生3840条稳定、清晰扩增带.五条扩增带在两基因池间呈现多态性,其中四条差异带出现在大铃基因池,编号分别为LB-1、LB-2、LB-3和LB-4;一条出现在小铃基因池,编号为SB-1.模板浓度梯度分析表明,在一定范围内,酶切模板浓度对AFLP结果影响较小.利用稍作修改的CTAB法提取的棉花DNA可用于AFLP分析.  相似文献   

伊犁马体尺年龄对其竞赛性能影响的研究   总被引：10，自引：1，他引：9  

姚新奎  裴红罗 《畜牧与兽医》2004,36(2):6-8

对伊梨马体尺、年龄与不同赛程成绩关系的研究表明:体高对各赛程影响极显著(P<0.01),但随赛程增长,影响逐渐减弱;胸围和管 围对各赛程影响显著(P<0.05);年龄对长赛程影响显著(P<0.05)。  相似文献   

Effect of Some Physical Properties of Breeder Egg on Hatchability of Cherry Valley Duck     

SONG Yuan-jian  PEI Dong-sheng  SUN Ya-feng  FANG Xing-tang 《湖北农业科学》2008,47(12)

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Change of reactive oxygen species production in co-culture model of rat astrocytes and motor neurons VSC 4.1     

TANG Lu  PEI Yue-hong  ZHANG Nan  LE Wei-dong  FAN Dong-sheng  DENG Min  ZHANG Jun 《园艺学报》2009,25(12):2441-2446

AIM: To explore the mutual effect of co-culture of wild-type astrocytes (ASC) and motor neurons VSC4.1 (VSC) on the respective ability to produce reactive oxygen species (ROS). METHODS: The inhibition rates of cell growth in ASC and VSC in co-culture or independent culture was detected after exposed to excitatory stimulus by MTT method. Real-time observation of ROS production by ASC and VSC labeled with Hoechst 33342 was detected by confocal microscopy under the conditions of co-culture or independent culture. RESULTS: Higher concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in ASC alone, while lower concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in VSC only. Real-time observation by confocal microscopy showed that ROS production by VSC under the condition of co-culture, which showed a notable increase at 15 min, was significant less than that in independent culture, which peaked at 5 min and was gradually decreased. ROS production by ASC in co-culture began to increase significantly at 10 min. CONCLUSION: Compared to independent culture, ASC reduces the resting ROS production by co-cultured with VSC, while ASC prolongs the duration of ROS production by VSC after exposed to excitatory stimulus.  相似文献   

高校信息化建设和管理模式的探讨     

王健  裴喜春 《农业网络信息》2009,(1)

本文针对高校信息化建设和管理中存在的问题以及笔者在高校信息化建设和管理中的亲身感受,在认真思考的基础上,提出了建设和管理统一化的设想.  相似文献   

不同前处理对测定甜高粱茎秆汁液3种糖含量的影响     

周振祥  赵博  王唯先  仝骁鹏  郑剑波  李艳红  高建明  罗峰  孙守钧  裴忠有 《草业科学》2022,39(5):940-948

茎秆含糖量是甜高粱（Sorghum dochna）重要的农艺性状,其可溶性糖的组分与含量的测定,是甜高粱QTL定位和性状改良的前提。本研究以粒用高粱的‘忻粱52’与甜高粱‘W452’重组自交系F8代255个群体为材料,采用改良蒽酮比色法来测定蔗糖、果糖和葡萄糖含量。结果表明：可溶性总糖的最佳显色时间为10 min,显色温度为100℃;测定蔗糖的最佳处理条件为1 mol·L^-1的KOH溶液处理10 min;果糖测定的最佳显色温度为50℃,显色时间为3 min。优化过后的蒽酮比色法能测得较稳定的数值,精密度和稳定性较好,可用于测定甜高粱汁液的糖含量。重组自交系后代糖锤度与可溶性糖总量之间呈极显著正相关关系（P <0.01）,相关系数为0.885,线性回归方程为y=37.082×可溶性糖总量+3.990。  相似文献