B‐cell lymphoma with Mott cell differentiation in two young adult dogs     

Nicole I. Stacy  Mary B. Nabity  Nicole Hackendahl  Melanie Buote  Jennifer Ward  Pamela E. Ginn  William Vernau  William L. Clapp  John W. Harvey 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(1):113-120

Abstract: Two young adult dogs with gastrointestinal signs were each found to have an intra‐abdominal mass based on physical examination and diagnostic imaging. On exploratory laparotomy, small intestinal masses and mesenteric lymphadenopathy were found in both dogs; a liver mass was also found in dog 1. Cytologic and histologic examination of intestinal and liver masses and mesenteric lymph nodes revealed 2 distinct lymphoid cell populations: lymphoblasts and atypical Mott cells. With Romanowsky stains, the atypical Mott cells contained many discrete, clear to pale blue cytoplasmic inclusions consistent with Russell bodies that were positive by immunohistochemistry for IgM and CD79a in both dogs and for IgG in dog 2. The Mott cells and occasional lymphoblasts stained strongly positive with periodic acid‐Schiff. Using flow cytometric immunophenotyping in dog 1, 60% of peripheral blood mononuclear cells and 85% of cells in an affected lymph node were positive for CD21, CD79a, IgM, and MCH II, indicative of B‐cells. With electron microscopy, disorganized and dilated endoplasmic reticulum was seen in Mott cells in tumors from both dogs. Antigen receptor gene rearrangement analysis of lymph node and intestinal masses indicated a clonal B‐cell population. Based on cell morphology, tissue involvement, and evidence for clonal B‐cell proliferation, we diagnosed neoplasms involving Mott cells. To the authors' knowledge, this is the second report of Mott cell tumors or, more appropriately, B‐cell lymphoma with Mott cell differentiation, in dogs. More complete characterization of this neoplasm requires further investigation of additional cases. This lymphoproliferative disease should be considered as a differential diagnosis for canine gastrointestinal tumors.  相似文献   

HPLC-DAD/MS characterization of flavonoids and hydroxycinnamic derivatives in turnip tops (Brassica rapa L. Subsp. sylvestris L.)     

Romani A  Vignolini P  Isolani L  Ieri F  Heimler D 《Journal of agricultural and food chemistry》2006,54(4):1342-1346

Flavonoids and hydroxycinnamic derivatives of turnip tops (Brassica rapa L. subsp. sylvestris L.) were characterized for the first time in four samples from different origins. Turnip tops exhibit a high polyphenols content (ranging from 107 to 191 mg/100 g, fresh weight) and a good antiradical activity, determined with the DPPH* test. After a liquid-liquid extraction and fractionation procedures, most flavonoids (isorhamnetin, kaempferol, and quercetin glycosides) and hydroxycinnamic derivatives were identified by means of HPLC-DAD/MS techniques. Isorhamnetin glycosides were the main flavonoid derivatives, differing from that found in the vegetables belonging to the Brassica oleracea group.  相似文献   

Within-field variability of Fusarium head blight pathogens and their associated mycotoxins   总被引：1，自引：0，他引：1  

X.-M. Xu  D. W. Parry  P. Nicholson  M. A. Thomsett  D. Simpson  S. G. Edwards  B. M. Cooke  F. M. Doohan  S. Monaghan  A. Moretti  G. Tocco  G. Mule  L. Hornok  E. Béki  J. Tatnell  A. Ritieni 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(1):21-34

Within-field variability in the Fusarium head blight (FHB) and its associated mycotoxins was studied in four European countries. At each of 14 sites, each FHB pathogen and associated mycotoxins were quantified in 16 quadrat samples at harvest. Overall, the incidence of quadrat samples with detectable and quantifiable pathogen DNA was significantly lower in the grain than in the corresponding chaff. Deoxynivalenol (DON) was the most frequently detected toxin in the samples and its accumulation was most strongly associated with the presence of Fusarium graminearum. Nivalenol (NIV) accumulation was significantly associated only with the presence of F. culmorum. Zearalenone (ZON) accumulation was strongly associated with the presence of all three pathogens (F. graminearum, F. culmorum and F. poae). The levels of both DON and ZON concentrations were positively related to the amount of F. graminearum DNA in the grain or in the chaff. The presence/absence of FHB pathogens within a single quadrat appeared to be independent of each other. The presence of a particular FHB pathogen and the amount of its DNA, as well as the associated mycotoxin(s), varied greatly among samples at each site. This study demonstrated the large extent of within-field variability of FHB and its associated mycotoxins, and the importance of representative sampling in FHB studies.  相似文献   

Environmental associations with broad‐scale Japanese and Taiwanese pelagic longline effort in the southern Indian and Atlantic Oceans          下载免费PDF全文

Pamela E. Michael  Geoffrey N. Tuck  Peter Strutton  Alistair Hobday 《Fisheries Oceanography》2015,24(5):478-493

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Clinical and histopathological features of myofibrillar myopathy in Warmblood horses          下载免费PDF全文

S. J. Valberg  A. M. Nicholson  S. S. Lewis  R. A. Reardon  C. J. Finno 《Equine veterinary journal》2017,49(6):739-745

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Effect of microbial‐based inoculants on nutrient concentrations and early root morphology of corn (Zea mays)     

Pamela Calvo  Dexter B. Watts  Joseph W. Kloepper  H. Allen Torbert 《植物养料与土壤学杂志》2017,180(5):636-636

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Effect of microbial‐based inoculants on nutrient concentrations and early root morphology of corn (Zea mays)     

Pamela Calvo  Dexter B. Watts  Joseph W. Kloepper  H. Allen Torbert 《植物养料与土壤学杂志》2017,180(1):56-70

Microbial‐based inoculants have been reported to stimulate plant growth and nutrient uptake. However, their effect may vary depending on the growth stage when evaluated or fertilizer applied. Thus, the objective of this study was to test the hypothesis that microbial‐based inoculants known to promote root growth and nutrient uptake will promote plant growth, enhance early root development, and increase nutrient concentrations of corn (Zea mays L.). Plants were evaluated at four different growth stages and in the presence of three different nitrogen (N) fertilizers. The microbial‐based treatments evaluated were: SoilBuilder™ (SB), a filtered metabolite extract of SoilBuilder™ (SBF), a mixture of four strains of plant growth‐promoting Bacillus spp (BM), and a water‐inoculated control. The experiment also included four fertilizer treatments: urea (U), urea‐ammonium nitrate (UAN), calcium‐ammonium nitrate (CAN), and an unfertilized control. Corn plants were evaluated at growth stages V2, V4, V6, and VT. Plant growth parameters for biomass, height, and SPAD readings were enhanced by the three microbial‐based treatments. A greater effect of microbial‐based treatments was observed when plants were evaluated at V6 and VT stages. Parameters of early root development such as total root length (TRL), root surface area (RSA), and length of fine roots were enhanced when microbial‐based treatments were applied. Concentrations of N, P, and K were also increased by microbial‐based treatments compared to the non‐inoculated control. Increases in plant N concentration due to microbial‐based treatments were on average 72% for CAN, 61% for UAN, 72% for urea, and 54% for the unfertilized control. Phosphorus concentration was increased most (138%) when BM was applied with CAN. In the same way, when CAN was present, K concentration was increased by 95% with BM and 65% when SB and SBF were applied. Overall, the results demonstrate that microbial‐based inoculants evaluated in this study can positively impact corn growth and nutrient concentration, especially during the late vegetative stages. Furthermore, the results indicate that the enhancement of nutrient concentrations (N, P, and K) in this case was related to the capacity of microbial‐based treatments to impact root morphology at early stages of corn growth.  相似文献   

OsWRKY IIa Transcription Factors Modulate Rice Innate Immunity   总被引：3，自引：0，他引：3  

Ying Peng  Laura E. Bartley  Patrick Canlas  Pamela C. Ronald 《Rice》2010,3(1):36-42

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Evaluation of type I fusarium head blight resistance of wheat using non-deoxynivalenol-producing fungi     

N. Gosman†  Srinivasachary  A. Steed  E. Chandler  M. Thomsett  P. Nicholson 《Plant pathology》2010,59(1):147-157

A series of experiments was conducted to determine whether type I resistance (resistance to initial infection) to fusarium head blight (FHB) in wheat could be assessed using fungal species/isolates that do not produce deoxynivalenol (DON), a mycotoxin critical to the spread of Fusarium graminearum in the wheat spike. It was shown that, while the non-toxin-producing species Microdochium nivale and M. majus could infect following spray inoculation of wheat spikes, they were unable to spread within the spike following point inoculation. However, although these species might reveal type I resistance, they are not highly pathogenic towards wheat. A nivalenol (NIV)-producing isolate of F. graminearum caused high levels of disease following spray inoculation, but spread only very slowly within the spike and rarely induced bleaching above the point of inoculation. It is proposed that spray inoculation with an appropriate, aggressive, non-DON-producing FHB pathogen may be used to characterize type I resistance to complement point inoculation with a DON-producing isolate to assess type II resistance (resistance to spread within the spike).  相似文献   

Response of Potatoes to Soil-Applied Insecticides, Fungicides, and Herbicides     

Michael Thornton  Jeff Miller  Pamela Hutchinson  Juan Alvarez 《Potato Research》2010,53(4):351-358

Non-target impacts of pesticides are well documented in crops such as soybeans and corn. However, little effort has been made to evaluate these impacts in potatoes. Field trials were conducted at the Parma and Aberdeen Research and Extension Centers during 2005 and 2006 to evaluate the potential for systemic insecticides, fungicides, and herbicides to affect disease development, crop injury, and tuber yield. The first set of trials evaluated six insecticide treatments (aldicarb, oxamyl, phorate, imidacloprid, thiamethoxam, and non-treated) in a factorial combination with in-furrow application of the fungicide azoxystrobin. Azoxystrobin significantly decreased Rhizoctonia stem canker, while aldicarb and phorate tended to increase it compared to the non-treated control. There was a significant insecticide by fungicide interaction due to a bigger reduction in disease index by the fungicide when certain insecticides were used. Despite the association of these insecticides with increased disease incidence, there was no significant impact on yield. In the second set of trials, sulfentrazone herbicide was applied just prior to plant emergence to potatoes treated with the same six insecticides to evaluate the potential for the in-furrow treatments to increase plant injury. Sulfentrazone caused stunting and visible plant injury, but the level of plant injury was influenced by insecticide treatment in only one out of four trials.  相似文献