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排序方式: 共有148条查询结果,搜索用时 31 毫秒
21.
D Castro‐González M Álvarez J Muro MC Esteso P De Paz L Anel F Martínez‐Pastor 《Reproduction in domestic animals》2010,45(2):363-367
To try new acrosomal probes for assessing ram spermatozoa, we compared the LysoSensor? probe, which labels acidic organelles, with the frequently used peanut agglutinin acrosomal probe (PNA‐PE; phycoerythrin as fluorescent moiety). The previous microscopic observations showed a lack of relationship of LysoSensor? with acrosomal status. Semen was obtained from five rams and frozen in four pools. Each pool was analysed carrying out a triple staining propidium ioide/PNA‐PE/LysoSensor? Green DND‐189 to test acrosome labelling, and a double staining SYBR‐14/PI, to assess sperm viability. Stained samples were analysed by flow cytometry. All measurements were replicated. Data were processed using agreement and repeatability tests. LysoSensor? labelling did not agree with PNA (mean of differences: 30.8%; coefficient of agreement: 22.6%), confirming microscopic observations. Nevertheless, when LysoSensor? was compared with SYBR‐14/PI, the agreement was high (mean of differences: ?0.05%; coefficient of agreement: 5.07%). Repeatability of both methods was high and similar. LysoSensor? did not seem to specifically stain the acrosome, but it may accumulate in the cytoplasm and label viable spermatozoa. Therefore, LysoSensor? might not be used as an acrosomal probe in ram spermatozoa, but it could be used in other kind of studies, taking advantage of its pH sensitivity. 相似文献
22.
Ben de Jong Carlos Anaya Omar Masera Marcela Olguín Fernando Paz Jorge Etchevers René D. Martínez Gabriela Guerrero Claudio Balbontín 《Forest Ecology and Management》2010
In this paper we present the Mexican inventory of greenhouse gas (GHG) emissions from the land-use sector. It involved integration of forest inventory, land-use and soil data in a GIS to estimate the net flux of GHG between 1993 and 2002. 相似文献
23.
The electrical conductivity of the water within the soil pores (ECp) measured with the WET sensor, appears to be a reliable estimate of soil salinity. A methodology combining the use of the WET sensor along with geostatistics was developed to delimit and evaluate soil salinity within an irrigated area under arid to semiarid Mediterranean climate in SE Spain. A systematic random sampling of 104 points was carried out. The association between ECp and the saturation‐extract electrical conductivity (ECse) was assessed by means of correlation analysis. The semivariograms for ECp were obtained at three different soil depths. Interpolation techniques, such as ordinary kriging and cokriging, were applied to obtain ECp levels in the unknown places. For each one of the soil depths, a model able to predict ECse from ECp was developed by means of ordinary least squares regression analysis. A good correlation (r = 0.818, p < 0.001) between ECp and ECse was found. Spherical spatial distribution was the best model to fit to experimental semivariograms of ECp at 10, 30, and 50 cm soil depths. Nevertheless, cokriging using the ECp of an adjacent soil depth as an auxiliary variable provided the best results, compared to ordinary kriging. An analytical propagation‐error methodology was found to be useful to ascertain the contribution of the spatial interpolation and ordinary least squares analysis to the uncertainty of the ECse mapping. This methodology allowed us to identify 98% of the study area as affected by salinity problems within a rooting depth of 50 cm, with the threshold of ECse value at 2 dS m–1. However, considering the crops actually grown and 10% potential reduction yield, the soil‐salinity‐affected area decreased to 83%. The use of sensors to measure soil salinity in combination with geostatistics is a cost‐effective way to draw maps of soil salinity at regional scale. This methodology is applicable to other agricultural irrigated areas under risk of salinization. 相似文献
24.
Nilia de la Paz Mirna Fernndez Orestes Lpez Caridad Garcia Antonio Nogueira Leonid Torres Wilfredo Turio Jyrki Heinmki 《Marine drugs》2021,19(6)
We investigated a spray drying process for preparing water-soluble salts of high molecular weight chitosan (CH) intended for pharmaceutical excipient applications. CH was derived from chitin of marine lobster origin (Panulirus argus). The effects of organic acid (acetic or lactic acid) and the ratio (difference) of inlet/outlet air temperature (140/90 °C or 160/100 °C) on spray drying were studied. The yield of spray-dried CH salt powders ranged from 50% to 99% in laboratory and industrial-scale processes. The spray-dried dry powder of CH salts consisted of spherical agglomerated particles with an average diameter of 36.2 ± 7.0 µm (CH acetate) and 108.6 ± 11.5 µm (CH lactate). After dispersing the spray-dried CH salt powder samples in purified water, the mean particle sizes obtained for the CH acetate salts were 31.4 nm (batch A001), 33.0 nm (A002) and 44.2 nm (A003), and for the CH lactate salts 100.8 nm (batch L001), 103.2 nm (L002) and 121.8 nm (L003). The optimum process conditions for spray drying were found: an inlet air temperature of 160 ± 5 °C, an outlet temperature of 100 ± 5 °C and an atomizer disk rotational speed of 18,200 min−1. The X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) results confirmed the amorphous state of the CH salts. The 1H nuclear magnetic resonance (NMR) and Fourier transform infrared (FT-IR) spectra of CH acetate and lactate salts verified that the spray drying process does not affect the polymer backbone. In conclusion, both laboratory and industrial-scale spray drying methods for preparing water-soluble acid salts of CH are reproducible, and the physicochemical properties of the corresponding CH acid salts are uniform. 相似文献
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27.
A quick methodology to identify sexual seedlings in citrus breeding programs using SSR markers 总被引:8,自引:0,他引:8
In citrus breeding and genetics, it is very important to distinguish between zygotic and nucellar seedlings in order to eliminate
unwanted genotypes. Usually, isozyme marker shave been employed to determine the genetic origin of young plants. In this work
we propose the use of SSR markers as an alternative methodology and compare them with isozymes in this kind of screenings.
Two different populations were analysed: one derives from an interspecific cross and the other from selfing. We conclude that,
in most cases, microsatellites are more efficient than isozymic markers to identify the sexual origin of citrus seedlings,
given their higher level of polymorphism and the scarce number of polymorphic isozymes in some populations. We describe a
quick and efficient methodology for SSR analysis, including a fast DNA extraction in microcentrifuge tubes, and visualization
through silver staining, which eliminates the need for a labelling step.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
28.
29.
H Ungar-Waron J Brenner R Paz Z Trainin 《Veterinary immunology and immunopathology》1992,34(1-2):173-179
Circulating immune complexes (ICs) were detected in the sera of bovine leukemia virus (BLV)-seropositive cattle. Immune complexes were precipitated in 2.5% polyethylene glycol (PEG) and further dissociated. Bovine leukemia virus antigens, IgG and IgM molecules were detected after solubilization in the presence of sodium dodecyl sulphate, and quantitated by enzyme-linked immunosorbent assay (ELISA) assays. Mean values of IgG and IgM in BLV-containing ICs did not significantly differ from those obtained from ICs originating from BLV-seronegative animals. However, differences were found in the composition of ICs from older BLV-positive animals as compared to those obtained from young animals. The ratio of IgG/IgM was 5.02 in animals aged 5-10 years, while this ratio was 11.66 in animals of less than 5 years of age and 10.19 in controls. This might indicate a possible increase in the contribution of IgM molecules to the structural composition of ICs in BLV-infected cattle as related to age or stage of infection. 相似文献
30.
Mercedes Alvarez Luis Anel‐Lopez Juan Carlos Boixo Cesar Chamorro Marta Neila‐Montero Rafael Montes‐Garrido Paulino de Paz Luis Anel 《Reproduction in domestic animals》2019,54(Z4):32-40
Ovine artificial insemination (OAI) is not commonly performed because of specific problems related to semen application techniques, leading to highly variable results. The ideal methodology (frozen‐thawed semen/vaginal route) is unfeasible under field conditions due to the cervix morphology of the ewe, which prevents the process of intrauterine insemination necessary to obtain acceptable results. Currently, OAI commercial programmes use superficial cervical insemination, CAI (vaginal), with chilled semen (15°C) and intrauterine insemination, LAI (laparoscopic), with frozen‐thawed semen. The ability to improve upon these contrasting techniques may be derived from examining certain poorly studied factors such as insemination time, productive state of females and alternatives of seminal preservation, some of which we reviewed in this work. This interim solution will remain in use until AI by the vaginal route with frozen‐thawed semen is developed, but it poses new challenges in optimizing the freezing of the sperm and adapting the cervical (CAI) and/or transcervical intrauterine AI (TCAI). In this review, we address the current problems and evaluate their methodological (mechanical) and chemical (dilation) alternatives. Currently, TCAI is a methodologically complex technique with poor fertility results, so further studies are needed to improve the logistics of this procedure and the results of its application. 相似文献