Spawning Frequency Analysis of Wild and Pond-Reared Pacific White Shrimp Penaeus vannamei Broodstock under Large-Scale Hatchery Conditions     

Elena  Palacios Ilie S.  Racolta Acuacultores  de la Paz 《Journal of the World Aquaculture Society》1999,30(2):180-191

Second generation Penaeus vannamei broodstack, pond-reared and wild populations, were compared for spawning frequency and several variables associated with reproductive performance. For this purpose, 470 pond-reared and 237 wild-caught individually tagged females were stocked with males of the same origin in ten maturation tanks in a commercial hatchery, and several productivity variables were recorded over time. Results of the analysis indicate that wild shrimp had higher mating and spawning frequencies compared to pond-reared broodstock. The number of nauplii per spawn was higher for wild shrimp, but fertilization and hatching rates were higher for pond-reared spawners. A larger proportion of nonmating females were found in pond-reared shrimp; whereas, wild females had a higher potential for multiple matings. The spawn quality (fertilization and hatching rates, percentage of viable spawns, and number of nauplii) in consecutive matings did not decline substantially. The present study indicates that selection of females with multiple spawning capabilities can be an important commercial strategy to improve nauplii production over the short term. Pond-reared broodstock can be an adequate source for applying this strategy because of their year-round availability and an overall acceptable reproductive performance.  相似文献   

Effect of Several Antioxidants on Thawed Ram Spermatozoa Submitted to 37°C up to Four Hours     

M Mata‐Campuzano  M Álvarez‐Rodríguez  M Álvarez  L Anel  P de Paz  JJ Garde  F Martínez‐Pastor 《Reproduction in domestic animals》2012,47(6):907-914

Thawed ram spermatozoa were incubated at 37°C in the presence of dehydroascorbic acid (DHA), TEMPOL (TPL), N‐acetyl‐cysteine (NAC) and rutin (RUT), at 0.1 and 1 mm , in order to test their effects on sperm physiology. Cryopreserved spermatozoa from four rams were thawed, pooled, washed and incubated in TALP‐Hepes with 1 mm or 0.1 mm of each antioxidant, performing a replicate with induced oxidative stress (Fe²⁺/ascorbate). Motility (CASA), viability and mitochondrial membrane potential (flow cytometry) were analysed at 2 and 4 h. Lipoperoxidation (MDA production), intracellular reactive oxygen species (ROS) and DNA status (TUNEL) were analysed at 4 h. Antioxidants, except DHA 0.1 mm , decreased motility and kinematic parameters, but had little effect on viability or mitochondrial activity. Except 1 mm DHA, the antioxidants reduced ROS at 4 h. Moreover, NAC 1 mm , rutin and TEMPOL reduced ROS and DNA damage in the presence of oxidative stress. N‐acetyl‐cysteine, rutin 1 mm and TEMPOL reduced lipoperoxidation in the presence of oxidative stress. However, DHA did not affect lipoperoxidation. At 1 mm , DHA increased DNA damage in the absence of oxidative stress. Dehydroascorbic acid effects could arise from spermatozoa having a low capacity for reducing it to ascorbic acid, and it may be tested in the presence of other antioxidants or reducing power. Future research should focus in testing whether the inhibition of motility observed for NAC, rutin and TEMPOL is reversible. These antioxidants might be useful at lower temperatures (refrigerated storage or cryopreservation) when their protective effects could be advantageous.  相似文献   

Effect of Saccharomyces cerevisiae var. Boulardii and β-galactomannan oligosaccharide on porcine intestinal epithelial and dendritic cells challenged in vitro with Escherichia coli F4 (K88)     

Badia R  Zanello G  Chevaleyre C  Lizardo R  Meurens F  Martínez P  Brufau J  Salmon H 《Veterinary research》2012,43(1):4

ABSTRACT: Probiotic and prebiotics, often called "immune-enhancing" feed additives, are believed to deal with pathogens, preventing the need of an immune response and reducing tissue damage. In this study, we investigated if a recently developed β-galactomannan (βGM) had a similar protective role compared to Saccharomyces cerevisiae var. Boulardii (Scb), a proven probiotic, in the context of enterotoxigenic Escherichia coli (ETEC) infection. ETEC causes inflammation, diarrhea and intestinal damage in piglets, resulting in large economic loses worldwide. We observed that Scb and βGM products inhibited in vitro adhesion of ETEC on cell surface of porcine intestinal IPI-2I cells. Our data showed that Scb and βGM decreased the mRNA ETEC-induced gene expression of pro-inflammatory cytokines TNF-α, IL-6, GM-CSF and chemokines CCL2, CCL20 and CXCL8 on intestinal IPI-2I. Furthermore, we investigated the putative immunomodulatory role of Scb and βGM on porcine monocyte-derived dendritic cells (DCs) per se and under infection conditions. We observed a slight up-regulation of mRNA for TNF-α and CCR7 receptor after co-incubation of DC with Scb and βGM. However, no differences were found in DC activation upon ETEC infection and Scb or βGM co-culture. Therefore, our results indicate that, similar to probiotic Scb, prebiotic βGM may protect intestinal epithelial cells against intestinal pathogens. Finally, although these products may modulate DC activation, their effect under ETEC challenge conditions remains to be elucidated.  相似文献   

Effect of Saccharomyces cerevisiae var. Boulardii and ��-galactomannan oligosaccharide on porcine intestinal epithelial and dendritic cells challenged in vitro with Escherichia coli F4 (K88)     

Roger Badia  Galliano Zanello  Claire Chevaleyre  Rosil Lizardo  Fran?ois Meurens  Paz Mart��nez  Joaquim Brufau  Henri Salmon 《Veterinary research》2012,43(1):4

  相似文献   

Distribution Pattern of Neuropeptide Y in the Brain, Pituitary and Olfactory System during the Larval Development of the Toad Rhinella arenarum (Amphibia: Anura)     

T. Heer    A. G. Pozzi    C. A Yovanovich    D. A. Paz 《Anatomia, histologia, embryologia》2009,38(2):89-95

The first NPY-immunoreactivity (ir) in the central nervous system of Rhinella arenarum was obtained just after hatching in the pre-optic area, ventral thalamus and rostral rhombencephalon. During pre-metamorphosis, new NPY-ir cells were observed in other brain areas such as pallium, septum and striatum, infundibulum and pars intermedia of the pituitary. Further maturation continued through pro-metamorphosis with the appearance of cell groups in the diagonal band, amygdala, pre-optic nucleus, dorsal nucleus of the habenula, anterior ventral and dorsal thalamus, suprachiasmatic nucleus, tuberculum posterior, tectum, torus semicircularis, inter-peduncular nucleus and median eminence. During the metamorphic climax and soon after, the relative abundance of NPY-ir fibres decreased in all hypothalamic areas and the staining intensity and number of NPY-ir cells in the pallium also decreased, whereas no cells were found in the striatum, dorsal nucleus of the habenula and tectum. In the olfactory epithelium, nerve or bulb, neither cells nor NPY-ir fibres were found during the stages of development analysed. The ontogeny pattern of the NPY-ir neuronal system in the brain of Rh. arenarum is more similar to the spatiotemporal appearance reported for Rana esculenta than to that reported for Xenopus laevis . Many NPY-ir fibres were found in the median eminence and in the pars intermedia of the pituitary, supporting the idea that this neuropeptide may play a role in the modulation of hypophyseal secretion during development.  相似文献   

Rabies virus and Histoplasma suramericanum coinfection in a bat from southeastern Brazil     

Benedito Donizete Menozzi  Giselle Souza da Paz  Laís Moraes Paiz  Hans Garcia Garces  Brunna Mayla Vasconcelos Adorno  Fernando Almeida‐Silva  Rosely Maria Zancope Oliveira  Virgínia Bodelo Richini‐Pereira  Jssica Luana Chechi  Eduardo Bagagli  Sandra de Moraes Gimenes Bosco  Helio Langoni 《Zoonoses and public health》2020,67(2):138-147

Bats are essential to the global ecosystem, but their ability to harbour a range of pathogens has been widely discussed, as well as their role in the emergence and re‐emergence of infectious diseases. This paper describes the first report of coinfection by two zoonotic agents, rabies virus (RABV) and the fungus Histoplasma suramericanum in a bat. The bat was from the Molossus molossus species, and it was found during the daytime in the hallway of a public psychiatric hospital in a municipality in São Paulo State, southeastern Brazil. RABV infection was diagnosed by the direct fluorescent antibody test and mouse inoculation test. The fungus was isolated by in vitro culture. Both diagnoses were confirmed by molecular techniques. Phylogenetic analysis showed that the fungus isolate had proximity to H. suramericanum in the Lam B clade, while the RABV isolate was characterized in the Lasiurus cinereus lineage. Since the M. molossus bat was found in a peri‐urban transition area (urban/peri‐urban), the possibility of cross‐species transmission of this RABV lineage becomes more plausible, considering that this scenario may provide shelter for both M. molossus and L. cinereus. These are relevant findings since there has been an increase in bat populations in urban and peri‐urban areas, particularly due to environmental modifications and anthropogenic impacts on their habitat. Thus, the detection of two zoonotic agents in a bat found in a public hospital should raise awareness regarding the importance of systematic surveillance actions directed towards bats in urban areas.  相似文献   

Tools for optimizing management of spatially-variable fields   总被引：5，自引：0，他引：5  

H. W. G. Booltink  B. J. van Alphen  W. D. Batchelor  J. O. Paz  J. J. Stoorvogel  R. Vargas 《Agricultural Systems》2001,70(2-3):445-476

Efficient use of agro-chemicals is beneficial for farmers as well as for the environment. Spatial and temporal optimization of farm management will increase productivity or reduce the amount of agro-chemicals. This type of management is referred to as Precision Agriculture. Traditional management implicitly considers any field to be a homogeneous unit for management: fertilization, tillage and crop protection measures, for example, are not varied within a single field. The question for management is what to do when. Because of the variability within the field, this implies inefficient use of resources. Precision agriculture defines different management practices to be applied within single, variable fields, potentially reducing costs and limiting adverse environmental side effects. The question is not only what and when but also where. Many tools for management and analysis of spatial variable fields have been developed. In this paper, tools for managing spatial variability are demonstrated in combination with tools to optimize management in environmental and economic terms. The tools are illustrated on five case studies ranging from (1) a low technology approach using participatory mapping to derive fertilizer recommendations for resource-poor farmers in Embu, Kenya, (2) an example of backward modelling to analyze fertilizer applications and restrict nitrogen losses to the groundwater in the Wieringermeer in The Netherlands, (3) a low-tech approach of precision agriculture, developed for a banana plantation in Costa Rica to achieve higher input use efficiency and insight in spatial and temporal variation, (4) a high-tech, forward modelling approach to derive fertilizer recommendations for management units in Zuidland in The Netherlands, and (5) a high-tech, backward modelling approach to detect the relative effects of several stress factors on soybean yield.  相似文献   

Chemical and sensorial characteristics of espresso coffee as affected by grinding and torrefacto roast     

Andueza S  De Peña MP  Cid C 《Journal of agricultural and food chemistry》2003,51(24):7034-7039

Grinding is a critical step in the preparation of espresso coffee (EC). The addition of sugar during the torrefacto roasting process could influence the degree of brittleness and grinding. The aim of this work was to study the influence of the grinding grades (coarse, fine, and very fine) in Arabica/Robusta 20:80, natural roasted (A20:R80), and Arabica/Robusta 20:80 with 50% Robusta torrefacto roasted (A20:R80 50% torrefacto) on the chemical and sensorial characteristics of EC in order to select the optimal espresso grinding grade. A higher percentage of coarse particles was found in A20:R80 ground coffee. In both ECs, the extraction of solids and soluble and aroma compounds increased inversely with particle size. Higher foam indices and extraction yields were found in A20:R80 50% torrefacto ECs probably due to the solubilization of caramelized sugar and melanoidins. It has been suggested that the range of an acceptable extraction yield could be extended to 25% in A20:R80 50% torrefacto ECs. In conclusion, the optimal grinding grade for the obtainment of an EC with A20:R80 was fine and that for A20:R80 50% torrefacto was coarse.  相似文献   

Simultaneous determination of (45)calcium and (65)zinc uptake by caco-2 cells     

Etcheverry P  Wallingford JC  Miller DD  Glahn RP 《Journal of agricultural and food chemistry》2002,50(22):6287-6294

A simple method for simultaneously determining cell-associated Ca and Zn in Caco-2 cells is described. Calcium and zinc uptake was measured via radioisotopes (45)Ca and (65)Zn. Preliminary studies revealed that (65)Zn, a positron (beta(+)) and gamma emitter, contributed to (45)Ca counts in a liquid scintillation counter (LSC). However, (45)Ca, being a true beta emitter, did not contribute to the counts in a gamma counter (gammaC). To differentiate the counts of (45)Ca from those of (65)Zn, first a (65)Zn-labeled cell suspension was read in a gammaC and an LSC, thus obtaining the relationship between the radioactive counts obtained from the gammaC and LSC. This information defined the linear relationship between gammaC (65)Zn counts per minute (CPM) and LSC (65)Zn CPM. Because the (45)Ca and (65)Zn counts obtained in the LSC are additive, giving total LSC CPM, the value of LSC (45)Ca CPM was obtained by subtracting LSC (65)Zn CPM from total LSC CPM for the dual-labeled cell sample, obtaining then LSC (45)Ca CPM. To determine the absolute activity or disintegrations per minute (DPM) of each isotope in the dual-labeled sample, the linear relationship between DPM and CPM was determined for each isotope. The method is simple and straightforward for the determination of (45)Ca counts from a sample also containing (65)Zn, using gamma and liquid scintillation counters.  相似文献   

Statistical Methods for Evaluating Results from Soil Micronutrient Analyses in Interlaboratory Programs     

Rosane da Silva Dias  Monica Ferreira de Abreu  Antonio Paz González 《Communications in Soil Science and Plant Analysis》2015,46(4):57-71

Interlaboratory comparison programs have been found to be useful to investigate potential error sources and therefore to remove or minimize their effects. A proficiency test was performed for five soil micronutrients [boron (B), iron (Fe), manganese (Mn), copper (Cu), and zinc (Zn)] by forty-nine independent Brazilian laboratories on four duplicated soil samples. Extraction of B was performed by hot water, whereas Fe, Mn, Cu, and Zn were extracted by diethylenetriaminepentaacetic acid (DTPA). The objectives were to compare the performance of different statistical methods for assessing between-laboratory variability (reproducibility) and to quantify the variability within single laboratories (repeatability). The statistical methods employed to compare interlaboratory analytical results were (1) Agronomic Institute method (mean, standard deviation, coefficient of variation), (2) Z₁ score (mean and standard deviation, including outliers), (3) Z₂ score (mean and standard deviation, excluding outliers), (4) robust Z score (median and standardized interquartile amplitude) denoted as Z₃, (5) Cochran test, and (6) Cochran + Grubbs tests. In addition, the robust Z-score method, denoted as Z₄, was used to assess intralaboratory variability. The performance of any laboratory for the analysis of soil micronutrients varied depending on the statistical method applied. The percentage of laboratories providing satisfactory results was 79 percent (Agronomic Institute), 95 percent (Z₁), 92 percent (Z₂), 89 percent (robust Z₃ score), 97 percent (Cochran), and 95 percent (Cochran + Grubbs). The Z-score tests become stronger and more rigorous when outliers were excluded (Z₂) or when the standardized interquartile amplitude was used (Z₃). Cochran and Grubbs tests, which are based on the removal of extreme values, were weaker than the other tests evaluated. In conclusion, the method employed by the proficiency test of the Agronomic Institute for removing outliers has been found to be the strongest and strictest one, yielding a reliable comparison of the results from soil micronutrient analyses.  相似文献