Ovine sperm DNA oxidation quantification using an 8‐OHdG immunodetection assay     

Pedro Javier Soria‐Meneses  Alejandro Jurado‐Campos  Vidal Montoro  Ana Josefa Soler  Jos Julin Garde  María del Rocío Fernndez‐Santos 《Reproduction in domestic animals》2019,54(Z4):59-64

Our aim was to optimize 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) immunodetection in order to detect DNA damage caused by oxidative stress that may not be detected by other DNA integrity analysis techniques, especially due to the high compaction of DNA in ruminants. Semen samples from 6 rams were cryopreserved. After thawing, samples were subjected to the DNA oxidation quantification using an 8‐OHdG immunodetection assay by flow cytometry. We have evaluated two different incubation times (30 min vs. overnight) at 4°C of the primary antibody (monoclonal anti‐8‐OHdG antibody). We have also compared the results of this technique with the sperm chromatin structure assay (SCSA^®). The analysis revealed that there were no significant differences (p > .05) between different incubation times. However, overnight incubation seems to cause more non‐specific binding of the secondary antibody. Significant differences (p < .05) between subjects and oxidation controls (8 M H₂O₂/800 μM FeSO₄?7H₂O) were evident. We can conclude that the 8‐OHdG immunodetection assay for DNA oxidation quantification of ram sperm can be performed subjecting sperm samples to a very high oxidative treatment.  相似文献   

Evaluation of conditions for in vitro storage of commercial and minor grapevine (Vitis vinifera L.) cultivars     

Tania San Pedro  Rosa Peiró  Carles Jiménez  Antonio Olmos 《The Journal of Horticultural Science and Biotechnology》2018,93(1):19-25

In vitro culture represents a tool for the ex situ conservation of a high number of sanitised plants in a reduced space. However, the culture media and/or other growing conditions need to be optimised to minimising plant growth and storage cost. Growth on MW medium was evaluated in the commercial cultivars ‘Airén’, ‘Bobal’, ‘Chardonnay’, ‘Garnacha Blanca’, ‘Moscatel de Alejandría’, ‘Moscatel de Grano Menudo’, ‘Pedro Ximénez’, ‘Pinot Blanc’, ‘Pinot Gris’, ‘Sauvignon Blanc’, and ‘Tempranillo’; the minor cultivars ‘Chelva’, ‘Valencí Negre’, ‘Valencí Blanc’, and ‘Verdil’; and the endangered cv. ‘Esclafacherre’. Different growth rates were observed among cultivars: those with faster growth need to be subcultured every 1.5–2.0 months; those with the slowest growth every 3.5–4.0 months. The effect of halving the sucrose in MW reduced the growth of the cultivars that grew faster without compromising survival. When IBA was removed from MW, growth was also reduced in some cultivars. Therefore, small modifications of the MW composition are adequate for grapevine in vitro storage under standard incubation conditions. This is an advantage with respect to the change of temperature used in other work to achieve growth reduction, and allows the use of the same chamber for different in vitro culture procedures.  相似文献   

Fat embolism secondary to yellow fat disease in an Appaloosa horse     

Alejandro Suárez-Bonnet  Antonio Espinosa de Los Monteros  Pedro Herráez  Francisco Rodríguez  Marisa Andrada  María José Caballero 《Journal of veterinary diagnostic investigation》2008,20(5):684-687

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Physiological effects of pruning in rose plants cv. Grand Gala     

Ángeles Calatayud  Dolors RocaElisa Gorbe  Pedro F. Martínez 《Scientia Horticulturae》2008

The effect of pruning in rose plants (cv. Grand Gala) was studied in different types of leaves, focusing on chlorophyll (Chl) a fluorescence, carbohydrates, ammonium content, nitrate reductase (NR) activity and biomass parameters. Results on pruned plants showed a higher maximum efficiency of photosystem II (PSII) of dark-adapted leaves, a higher actual quantum yield and a higher proportion of PSII reaction centres that are open, but a lower non-photochemical quenching, indicating a lower energy dissipation as heat, compared to non-pruned plants. These results related to Chl a fluorescence, indicate that pruned plants have a higher capacity for better promoting the photosynthetic light reaction than non-pruned plants. The increased nitrate reductase activity in pruned compared with non-pruned plants, can result from a higher photosynthetic activity resulting in a lower NH₄⁺ accumulation in leaves. Pruning promoted a large number of metabolic sinks (flower removal) that may cause depletion of stored carbohydrates flowing from lower plant parts (arched shoots) to the new developing flower shoots. However, although in a lower concentration, carbohydrate contents were sufficient to promote the development of new flower shoots since the yield was similar for pruned and non-pruned plants. However, pruned plants showed higher turgor than non-pruned plants.  相似文献   

Information Transfer in the Agricultural Sector in Spain     

Antonio Muñoz-Cañavate  Pedro Hípola 《Journal Of Agricultural & Food Information》2013,14(2):123-142

This article examines the structures of information transfer to the agricultural (production) and agro-alimentary (transformation and commercialization of the products) sector within Spain. An historical perspective is provided to better illustrate the reality and complexity of Spain with regard to the systems of agrarian extension, agricultural research, the resources provided by Spain's central administration, and the use of information by related enterprises.

The Service of Agrarian Extension appeared in Spain in the 1950s, and new political-administrative structures (agribusiness associations, cooperatives) were founded when Spain became a democratic nation in the late 1970s and with the arrival of electronic information, largely in the 1990s. We describe the research and technological centers supporting innovation in the agro-alimentary sector and the communication media dedicated to the agricultural sector. The article illustrates that the systems of agricultural information in Spain have been largely derived from initiatives of the Public Administration, with few private initiatives.  相似文献   

Use of Rhizobium leguminosarum as a potential biofertilizer for Lactuca sativa and Daucus carota crops     

José D. Flores‐Félix  Esther Menéndez  Lina P. Rivera  Marta Marcos‐García  Pilar Martínez‐Hidalgo  Pedro F. Mateos  Eustoquio Martínez‐Molina  Ma de la Encarnación Velázquez  Paula García‐Fraile  Raúl Rivas 《植物养料与土壤学杂志》2013,176(6):876-882

Microbial biofertilizers are becoming an effective tool for sustainable agriculture by means of the reduction of the use of chemical fertilizers. However, the knowledge of each specific plant–microorganism interaction is essential for a correct application. In this study, we analyzed the in vitro plant‐growth‐promotion mechanisms of a Rhizobium leguminosarum strain named PEPV16 isolated from Phaseolus vulgaris nodules. This strain was able to produce siderophores and indole acetic acid and to solubilize phosphate. Confocal microscopy showed that this strain was able to colonize the roots of two horticultural crops, Lactuca sativa L. (lettuce) and Daucus carota L. (carrot). Strain PEPV16 was also able to promote the plant growth of both plant species increasing the dry matter of shoots and roots of lettuce and carrots, respectively, as well as to increase the uptake of N and P in the edible parts of both plant species. These data confirmed the suitability of Rhizobium as biofertilizer for nonlegumes.  相似文献   

Short-term effects of organic and inorganic fertilizers on soil microbial community structure and function   总被引：1，自引：0，他引：1  

Cristina Lazcano  María Gómez-Brandón  Pedro Revilla  Jorge Domínguez 《Biology and Fertility of Soils》2013,49(6):723-733

A field study was carried out to analyze the short-term impacts of replacing mineral by organic fertilizers on the microbial and biochemical parameters relevant for soil fertility and crop yield. Three types of fertilization regimes were compared: (1) conventional fertilizer regime with inorganic fertilizer, and combined integrated fertilizer regimes in which 25 % of the nutrients were supplied by either (2) rabbit manure or (3) vermicompost. The effects on microbial community structure and function (phospholipid fatty acid [PLFA] profiles, bacterial growth, fungal growth, basal respiration, β-glucosidase, protease and phosphomonoesterase activities), soil biochemical properties (total C, dissolved organic carbon [DOC], N-NH₄ ⁺, N-NO₃ ^?, PO₄, total K) and crop yield were investigated in the samples collected from the experimental soil at harvest, 3 months after addition of fertilizer. The integrated fertilizer regimes stimulated microbial growth, altered the structure of soil microbial community and increased enzyme activity relative to inorganic fertilization. Bacterial growth was particularly influenced by the type of fertilizer regime supplied, while fungal growth only responded to the amount of fertilizer provided. The use of manure produced a fast increase in the abundance of PLFA biomarkers for Gram-negative bacteria as compared to inorganic fertilizer. Nutrient supply and crop yield with organic fertilizers were maintained at similar levels to those obtained with inorganic fertilizer. The effects of the organic amendments were observed even when they involved a small portion of the total amount of nutrients supplied; thereby confirming that some of the beneficial effects of integrated fertilizer strategies may occur in the short term.  相似文献   

Chemical Characterization of Sewage Sludges in Chile and Their Potential Utilization as Amendment to Reclaim Soils for Forestation Purposes     

S. Maria Aguilera  Gilda Borie  Pedro Peirano  Manueal Rodriguez  Ivan Grez  Hugo Zunino 《Journal of plant nutrition》2013,36(12):1993-2003

Soils open for forestation in Chile are characterized by their very low organic carbon content; therefore, new forest plantations, needed to restore soil ecological equilibria, are scarcely developed. Stabilized sewage sludge contain organic compounds which have been demonstrated can serve as good soil amendments. To evaluate their actual uses in Chile it is necessary to characterize the carbon (C)-distribution pattern of such sludges in order to foresee their potential contribution for soil reclamation and plant growth, acting both as a source of stable C-reservoir and as readily available C-source.

The molecular weight (m.w.) distribution and carbon balance in Chilean sewage sludges are quite similar to soil humus and some woody by-products, as sawdust and bark dust. Total C varies from 30 to 35%, N from 5 to 9%, and P from 10,000 to 12,000 ppm. No significant content was found for heavy metals.

The C-balance indicates that around 70% of total-C is under stabilized forms as humine and humic macromolecule structures. The sewage sludge can be considered as good amendments for forest soils, especially in areas heavily eroded and depleted of natural humus. Their soluble-C fractions (13% of total-C) will serve as good energy starter source to strengthen indigenous soil microbial ecology, while their high m.w. fractions (humic acid-like and humine-like macromolecules) will serve both as good humus reservoir and as microelement frame in soils treated with such sewage sludges.  相似文献   

Prediction,structure characterization,and evolutionary analysis of Erwinia psidii putative type III effectors     

Isadora C. Pereira  Jorge L. Badel  Pedro M. P. Vidigal  Adryelle A. Sousa  Samuel A. Santos  Lúcio M. S. Guimarães  Acelino C. Alfenas 《Plant pathology》2021,70(3):555-566

Erwinia psidii is a gram-negative bacterium that threatens both guava and eucalypt plantations in several countries. Despite the economic importance of both crops, nothing is currently known about the molecular mechanisms underlying E. psidii pathogenicity and, consequently, how it evolved to infect Eucalyptus species besides its presumed native host Psidium guajava. In this study, we predicted putative type III secretion system effectors that may play important roles during plant–E. psidii interactions and conducted effector structure and phylogenetic analyses to gain important insights into their function and evolution. For that, the whole genomes of four E. psidii strains that exhibit differential aggressiveness towards eucalypt clones were sequenced and their effector repertoires predicted based on sequence identity with known effectors of the model phytopathogen Erwinia amylovora. Only proteins sharing significant sequence identity with the DspE and Eop1 effectors were found. Here, it is shown that these two E. psidii effectors retain all structural characteristics of their corresponding protein superfamilies, but exhibit allelic variations that are consistent with the observed aggressiveness differences between strains. Phylogenetic analyses revealed that whereas E. psidii housekeeping gene sequences are more closely related to those from Erwinia tracheiphila, the effector (either nucleotide or amino acid) sequences are more closely related to their Pantoea agglomerans counterparts, suggesting that dspE and eop1 were both acquired through horizontal gene transfer from the latter bacterial species. The results of this study provide important insights on E. psidii pathogenicity and set the stage for future effector functional studies.  相似文献   

A Multi-Omics Characterization of the Natural Product Potential of Tropical Filamentous Marine Cyanobacteria     

Tiago Leo  Mingxun Wang  Nathan Moss  Ricardo da Silva  Jon Sanders  Sergey Nurk  Alexey Gurevich  Gregory Humphrey  Raphael Reher  Qiyun Zhu  Pedro Belda-Ferre  Evgenia Glukhov  Syrena Whitner  Kelsey L. Alexander  Robert Rex  Pavel Pevzner  Pieter C. Dorrestein  Rob Knight  Nuno Bandeira  William H. Gerwick  Lena Gerwick 《Marine drugs》2021,19(1)

Microbial natural products are important for the understanding of microbial interactions, chemical defense and communication, and have also served as an inspirational source for numerous pharmaceutical drugs. Tropical marine cyanobacteria have been highlighted as a great source of new natural products, however, few reports have appeared wherein a multi-omics approach has been used to study their natural products potential (i.e., reports are often focused on an individual natural product and its biosynthesis). This study focuses on describing the natural product genetic potential as well as the expressed natural product molecules in benthic tropical cyanobacteria. We collected from several sites around the world and sequenced the genomes of 24 tropical filamentous marine cyanobacteria. The informatics program antiSMASH was used to annotate the major classes of gene clusters. BiG-SCAPE phylum-wide analysis revealed the most promising strains for natural product discovery among these cyanobacteria. LCMS/MS-based metabolomics highlighted the most abundant molecules and molecular classes among 10 of these marine cyanobacterial samples. We observed that despite many genes encoding for peptidic natural products, peptides were not as abundant as lipids and lipopeptides in the chemical extracts. Our results highlight a number of highly interesting biosynthetic gene clusters for genome mining among these cyanobacterial samples.  相似文献