Multidrug Resistance in Botrytis cinerea Associated with Decreased Accumulation of the Azole Fungicide Oxpoconazole and Increased Transcription of the ABC Transporter Gene BcatrD     

Keisuke Hayashi  Henk-jan Schoonbeek  Hisao Sugiura  Maarten A. De Waard 《Pesticide biochemistry and physiology》2001,70(3):168

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Fusarium moniliforme secretes four endopolygalacturonases derived from a single gene product     

C. Caprari  C. Bergmann  Q. Migheli  G. Salvi  P. Albersheim  A. Darvill  F. Cervone  G. De Lorenzo 《Physiological and Molecular Plant Pathology》1993,43(6)

Extracellular endopolygalacturonase, purified from the pathogenic fungus Fusarium moniliforme, consists of four molecular forms (38, 41·5, 45, and 48·5 kDa, respectively. Three forms (38, 41·5, and 45 kDa) were purified to homogencity by FPLC on a Mono S column followed by electroelution after SDS-PAGE. The N-terminal amino acid sequences of each of the three forms, and of a mixture containing all four forms were shown to be identical to that predicted from the nucleotide sequence of the endopolygalacturonase gene previously cloned from F. moniliforme. Enzymatic deglycosylation experiments revealed the presence of N-linked, high mannose oligosaccharide side-chains on all four forms of endo polygalacturonase. Hydrogen fluoride catalysed chemical deglycosylation of the polygalacturonase mixture yielded a single polypeptide with an apparent molecular mass of 36·2 kDa. Southern blot analysis, carried out at high stringency with an endopolygalacturonase-specific probe on genomic DNA digested with three different restriction enzymes, showed a single hybridizing restriction fragment in all three digests. A single 2·0 Mb chromosome hybridized with the endo polygalacturonase-specific probe, as shown by Southern blot analysis of F. moniliforme chromosomes separated by CHEF electrophoresis. Northern blot analysis revealed only one mRNA species 1350 nt encoding endo polygalacturonase. These data indicate that a single gene encodes the endopolygalacturonases of F. moniliforme.  相似文献   

Encephalopathy with idiopathic hyperammonaemia and Alzheimer type II astrocytes in equidae     

Hasel KM  Summers BA  De Lahunta A 《Equine veterinary journal》1999,31(6):478-482

In 3 mature female horses of varying breeds, episodes of colic and depression for 14 days preceded an encephalopathic disorder with maniacal behaviour, anxiety, profuse sweating and, in one case, terminal opisthotonus. Blood ammonia levels were elevated approximately 10-fold. At necropsy, there were gastrointestinal serosal and mesenteric haemorrhages. Histologically, all 3 cases revealed diffuse Alzheimer type II astrocytes in the cerebral grey matter. Alzheimer type II astrocytes were glial fibrillary acidic protein (GFAP) negative or only weakly positive, weakly S-100 positive, and vimentin negative. In the absence of primary hepatic and/or renal lesions, an increase in intestinal ammonia absorption due to ileus or increased ammonia production by colonic bacteria is hypothesised.  相似文献   

Bacterial and fungal pathogens isolated from corneal ulcerations in domesticated elephants (Elephas maximus maximus) in Sri Lanka     

Kodikara DS  De Silva N  Makuloluwa CA  De Silva N  Gunatilake M 《Veterinary ophthalmology》1999,2(3):191-192

Infective corneal diseases are common among the domesticated elephants in Sri Lanka, causing morbidity including blindness leading to loss of revenue to their owners. From 140 elephants of different ages and both sexes, 36 animals (25.7%) had evidence of keratitis, corneal ulcers, corneal opacities, and some had foreign bodies in their eyes. Nine elephants (6.4%) had lesions in both eyes (6.41%). Cultures for both bacteria and fungi were obtained from 26 corneal ulcers, including the nine elephants with bilateral lesions. The other 10 animals could not be restrained for sample collection. Swabs from the normal corneas of an additional 20 elephants without signs of any ophthalmic diseases were also collected. Twenty-three of the 35 (65.71%) samples from affected corneas yielded bacterial pathogens, and 14 (40%) also had fungal isolates. None of them yielded a fungal isolate alone. The predominant bacteria isolated were Staphylococcus aureus , beta hemolytic streptococci and coliforms. Fusarium , Cladosporium , Curvularia and Aspergillus species were the primary fungal isolates. No bacteria or filamentous fungi were isolated from the eyes with the normal corneas. Microbial identification including fungal isolations is suggested in the management of infective corneal diseases in elephants.  相似文献   

Seasonal dynamics of the free-living phase of Anocentor nitens at Pedro Leopoldo, Minas Gerais, Brazil     

Borges LM  Oliveira PR  Ribeiro MF 《Veterinary parasitology》1999,87(1):73-81

An understanding of seasonal dynamics in the free-living phase of the tropical horse tick, Anocentor nitens, is vital for effective control measures. This study examines seasonal changes in the oviposition and incubation periods, eclosion rates, reproductive success, and larval survival in the free-living phase during the period June 1995-July 1997. Ten engorged female ticks, placed individually in wire-gauze tubes, were exposed in a field of Brachiaria decumbens fortnightly each month, and monitored to measure the duration of the various reproductive parameters, and success. Larval abundance was estimated indirectly, through the percentage of females with viable progeny, and directly by using a flagging method. Duration of the free-living cycle ranged from 12.3 weeks (October and December) to 23.5 weeks (March). The cycle was prolonged in cold and dry months. Larval survival was longer from February to July, and reduced from August to February. Oviposition was recorded in all months, and the egg production index ranged from 26.7 to 47.1%. The eclosion rate ranged from 0.1 to 76.6% in different months. Monthly estimates of larval abundance in the pasture were similar for the two methods used, being higher in months with higher temperatures, relative humidity and rainfall.  相似文献   

Bile duct hamartoma in a calf     

De Bosschere H  Ducatelle R 《The Veterinary record》1999,144(8):210-211

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福建高速公路生物护坡草坪的建植与养护   总被引：8，自引：2，他引：6  

余德亿  卢和顶  汤葆莎 《草业科学》2001,18(5):45-49

分析了福建高速公路所经区域的土壤、气候及草坪植被环境特点，归纳总结了高速公路生物护坡草坪的建植与养护技术，对草种选择，建植与养护，质量保障等进行讨论并提出了建议，旨在提高高速公路生物护坡草坪的建植与管理技术水平。  相似文献   

Determination and persistence of imazalil in post-harvest-treated apples during cold storage     

Maria P. Cano  Josê L. De la Plaza  Luis Muoz-Delgado 《Pest management science》1987,19(4):283-287

A method to determine imazalil (allyl 1 -(2, 4-dichlorophenyl)-2-imidazol- 1-ylethyl ether) on apples using high performance liquid chromatography is described. After harvest, fruits were immersed in an aqueous suspension (1 g litre-1) and cold stored (0-2°C) at 85-90% r.h. Samples were taken monthly and imazalil was determined in the peel, outer pulp, inner pulp and on whole fruit. Residues were 3.8-4.9 mg imazalil kg-1 and decreased during storage. Most imazalil was found on the peel and amounts in the pulp decreased toward the core.  相似文献   

Animal Physiologic Surgery          下载免费PDF全文

L. De Roth 《The Canadian veterinary journal. La revue veterinaire canadienne》1978,19(9):243

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Surgical and conservative treatment of infectious arthritis in cattle   总被引：1，自引：0，他引：1  

F Verschooten  A De Moor  M Steenhaut  P Desmet  L Wouters  G De Ley 《Journal of the American Veterinary Medical Association》1974,165(3):271-275

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