全文获取类型
收费全文 | 2545篇 |
免费 | 177篇 |
国内免费 | 130篇 |
专业分类
林业 | 163篇 |
农学 | 155篇 |
基础科学 | 97篇 |
393篇 | |
综合类 | 661篇 |
农作物 | 163篇 |
水产渔业 | 236篇 |
畜牧兽医 | 711篇 |
园艺 | 101篇 |
植物保护 | 172篇 |
出版年
2024年 | 7篇 |
2023年 | 37篇 |
2022年 | 86篇 |
2021年 | 102篇 |
2020年 | 116篇 |
2019年 | 100篇 |
2018年 | 105篇 |
2017年 | 137篇 |
2016年 | 87篇 |
2015年 | 94篇 |
2014年 | 134篇 |
2013年 | 169篇 |
2012年 | 184篇 |
2011年 | 169篇 |
2010年 | 132篇 |
2009年 | 118篇 |
2008年 | 156篇 |
2007年 | 130篇 |
2006年 | 102篇 |
2005年 | 91篇 |
2004年 | 64篇 |
2003年 | 52篇 |
2002年 | 71篇 |
2001年 | 62篇 |
2000年 | 55篇 |
1999年 | 41篇 |
1998年 | 9篇 |
1997年 | 18篇 |
1996年 | 11篇 |
1995年 | 21篇 |
1994年 | 6篇 |
1993年 | 15篇 |
1992年 | 7篇 |
1991年 | 15篇 |
1990年 | 11篇 |
1989年 | 11篇 |
1988年 | 12篇 |
1987年 | 10篇 |
1986年 | 10篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 6篇 |
1979年 | 6篇 |
1976年 | 6篇 |
1973年 | 4篇 |
1970年 | 5篇 |
1969年 | 4篇 |
1968年 | 6篇 |
1967年 | 8篇 |
1966年 | 4篇 |
排序方式: 共有2852条查询结果,搜索用时 31 毫秒
981.
982.
杨梅肌动蛋白基因MrActin1的克隆及表达分析 总被引:1,自引:0,他引:1
扩增杨梅嫩叶中肌动蛋白基因(MrActin1)全长编码区cDNA序列,并评价MrActin1的进化地位,同时分析MrActin1的表达模式。利用RT-PCR扩增MrActin1全长编码区cDNA序列。用生物信息学手段分析预测MrActin1的理化性质和同源性,用临接法构建其的系统发生树。通过Northern blot分析MrActin1在不同组织部位的表达。该基因cDNA序列(GenBank登录号AB 650589)全长1137 bp,编码了1个由377个氨基酸残基组成的蛋白质,所得序列与GenBank中注册的其他植物Actin氨基酸序列的相似性均在88%以上,根据高等植物Actin相似性构建了进化树,表明MrActin1与陆地棉、圆叶锦葵Actin之间的亲缘关系最为密切,在进化中分化时间最为接近。Northern blot分析表明,MrActin1在杨梅的花、叶、枝条和果组织中恒定表达。首次获得了杨梅MrActin1 cDNA全长序列。 相似文献
983.
984.
A. Lehtinen A. Hannukkala B. Andersson A. Hermansen V. H. Le R. Nærstad M. B. Brurberg B. J. Nielsen J. G. Hansen J. Yuen 《Plant pathology》2008,57(2):227-234
A total of 743 single-lesion isolates of Phytophthora infestans were collected in summer 2003 from Denmark, Finland, Norway and Sweden. Most of the isolates were tested for mating type, and subsets were tested for sensitivity to fungicides and virulence (host specific pathogenicity). Approximately 60% of the isolates were A1 mating type in each country. Both mating types were present in 40% of the fields where more than one isolate was tested, indicating strong potential for sexual reproduction. The proportion of metalaxyl-resistant isolates dropped to under 15% from the 60% observed in the early 1990s in Norway and Finland, possibly due to lower selection pressure because of decreased use of metalaxyl. Propamocarb-HCl sensitivity remained unchanged in the Nordic countries compared to the situation in 1997–2000 in Finland. Four isolates collected from Finland and Sweden were able to sporulate in the presence of this fungicide at a concentration of 1000 mg L−1 . In Norway and Finland the frequencies of virulence factors and pathotypes remained nearly unchanged since the 1990s, but the mean number of virulence factors per isolate increased from 5·6 to 6·3. In Denmark and Sweden virulence factors 2 and especially 6 were more common than in Norway and Finland. In addition, in the Swedish population the frequencies of pathotypes were quite even while in other countries pathotype 1,3,4,7,10,11 was most prevalent. 相似文献
985.
de Roffignac L Cattan P Mailloux J Herzog D Le Bellec F 《Pest management science》2008,64(12):1303-1313
BACKGROUND: After the rinsing of spray equipment, the rinsing water contains polluting products. One way to avoid pollution is to bring the rinsing water over a purification system, a biological bed. The system consists of an impermeable tub filled with a biomix substrate that facilitates biodegradation of pesticides. Usually, straw is one component of the biomix. The objective of this study was to assess the efficiency of an unusual substrate, bagasse, a residue of sugar cane, for the degradation of three pesticides, glyphosate, malathion and lambda‐cyhalothrin. RESULTS: Results showed that more than 99% of malathion and glyphosate were degraded in 6 months. In the biological bed, the DT50 value for malathion was 17 days, for glyphosate 33 days and for lambda‐cyhalothrin 43 days. The degradation rate of aminomethylphosphonic acid (AMPA) residues from the degradation of glyphosate was slower than that of the other pesticides (DT50 69 days). Finally, the innocuousness of the biomix after 6 months of degradation was confirmed by biological tests. CONCLUSIONS: Although the degradation rates of the three pesticides in the present bagasse‐based system were similar to those under temperate conditions, the degradation conditions were improved by comparison with those in soil under the given tropical conditions. Further benefits of this system are pesticide confinement, to avoid their dispersion in the environment by liquids or solids, and a lower overall cost. Finally, possibilities for optimising the bagasse‐based system (e.g. management of the water content and nature of the biomix) are discussed. Copyright © 2008 Society of Chemical Industry 相似文献
986.
Nam Cao Quoc Nico Vromant Duong Le Thanh Frans Ollevier 《Aquaculture International》2012,20(5):827-845
In recent years, a rotational system consisting of two rice crops followed by a fish pen culture during the monsoon flood period was adopted in parts of the flood-prone region of the Mekong Delta. In this paper, we use the survey data from 51 fish pen farmers at Co Do and Vinh Thanh district, Can Tho City, to investigate the factors affecting fish yield and profit in such fish pen culture system. The net yield of all stocked fish varies from 377 to 3,782?kg/ha/crop while the return above variable costs varies from ?5.3 to 9.8 million VND/ha/crop. Low fish price at harvest and the difficulty to sell below market sized fish at a period of oversupply are the main problems for fish pen culture. Fish net yield significantly increases with stocking density and is linked with specific polycultures. Common carp and bighead carp perform best in fish pen culture, both in production and profit considerations. Harvest body mass of common carp is mainly limited by increasing common carp stocking density. Therefore, a stocking density of common carp of about 6,000 fish/ha is recommended to obtain the optimum combination of reasonable body masses, and a good price, as well as a high return above fingerling costs. 相似文献
987.
988.
研究了不同的冻前处理工艺和冻结方式对甜玉米和豌豆低温保存的影响,并探讨了其影响机理;后用不同的解冻方法对甜玉米和豌豆进行解冻,并分析其对甜玉米和豌豆品质的影响。结果表明,在超低温冰箱保存贮藏的甜玉米和豌豆的质构、颜色、营养、风味变化幅度较小,品质明显优于普通冻藏。 相似文献
989.
990.