Ultrastructural characteristics of the blood cells of chickens commonly reared under backyard poultry farming in Mizoram,India     

Probal Jyoti Doley  Kabita Sarma  Pranab Chandra Kalita  Ranjana Goswami  Arup Kalita  Rupan Sarkar  Chethan Gollahalli Eregowda  Parimal Roychoudhary  Om Prakash Choudhary 《Anatomia, histologia, embryologia》2023,52(2):223-233

The present study was undertaken to characterize the ultrastructural morphology of the blood cells of commonly reared chickens in the state of Mizoram, India under backyard poultry farming. For this study, 2 ml of whole blood was aseptically collected from the wings veins of 12 chickens of three different breeds namely the Zoar, Aseel and Rhode Island Red and processed for ultrastructural imaging under standard protocols. Under scanning electron microscopy (SEM) the matured erythrocytes of Zoar, Aseel and Rhode Island Red appeared elliptical in shape while the leukocytes and thrombocytes appeared round in shape with variable surface modifications. Under transmission electron microscopy (TEM) the granules of the heterophils of Zoar, Aseel and Rhode Island Red appeared predominantly fusiform in shape, the granules of the eosinophils appeared round in shape and that of the basophils appeared pleomorphic in shape. The cytoplasm of the monocytes, medium-to-large lymphocytes and thrombocytes of Zoar, Aseel and Rhode Island Red under TEM appeared to be vacuolated and granular while that of the small lymphocytes appeared to be granular but non-vacuolated. The study concluded that the ultrastructural characteristics of the blood cells of the three breeds of chicken studied were almost similar to the blood cells of other birds reported earlier.  相似文献   

Present and future feasibility of short-rotation energy farms in Ontario     

Lesley A. Moran  Jagdish C. Nautiyal 《Forest Ecology and Management》1985,10(4):323-338

The economic feasibility of energy farming based on Populus × euramericana (clone I45/51) in eastern Ontario has been evaluated for two potential energy end-uses: direct combustion and methanol production. Optimal management regime and the time when energy farming should begin to be economically most desirable have been determined. Three-year rotations with 16–35 thousand stumps/ha planted and coppiced for 5–6 rotations could be an attractive enterprise in about 15 years. Farming for methanol production could be feasible only about 35 years from now.  相似文献   

Micropropagation of red sanders (Pterocarpus santalinus L.) using mature nodal explants     

Ekambaranellore Prakash  Patan Shaik Sha Valli Khan  Thoguru Jonh Vivek Sreenivasa Rao  Elu Singh Meru 《Journal of Forest Research》2006,11(5):329-335

In vitro clonal multiplication of Pterocarpus santalinus L. was achieved using mature nodal explants of a 10-year-old elite quality tree. Combinations of serial transfer technique and incorporation of antioxidants (250 mg/l L-ascorbic acid and 50 mg/l citric acid) into the culture medium helped to minimize medium browning and improve explant survival during shoot sprouting. About 70% of explants were sprouted on Murashige and Skoog (MS) liquid medium containing 4.4 μM 6-benzyladenine (BA). The explant harvest period also influenced the bud break and shoot sprouting in nodal explants. The combination of 4.4 μM BA and 2.2 μM thidiazuron (TDZ) was found to be the most suitable growth regulator for obtaining the highest percentage of nodal segment sprouting (74%–75%), the number of secondary shoots per primary shoot (two or three), the shoot length (5–6 cm), the number of new nodal segments generated per active explant (four or five), and the multiplication coefficient (3.5) within 6 weeks. Repeated subculturing of nodal explants obtained from shoot cultures enabled continuous production of healthy axillary shoots. At the end of the sixth passage, about 90% of nodal explants produced five or six healthy green shoots, each being about 6.6 cm long with six or seven nodes. Multiplication coefficient was also increased from the first subculture (5.4) to the sixth subculture (8.3). The best rooting response was achieved on solidified half-strength MS medium supplemented with 4.9 μM indole-3-butyric acid (IBA). About 70% of the micropropagated plantlets were established successfully in 20-cm pots containing a mixture of soil and farmyard manure (4 : 1 ratio) and formed new leaflets.  相似文献   

Purification and characterization of a new endoglucanase from Aspergillus aculeatus     

Naika GS  Kaul P  Prakash V 《Journal of agricultural and food chemistry》2007,55(18):7566-7572

Endoglucanase has been isolated from Aspergillus aculeatus. The purified enzyme showed a single band and had a molecular weight of 45,000 Da as indicated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with a specific activity of 1.4 units/mg. The purified enzyme was identified as endoglucanase, showing a high specific activity toward CM-cellulose and low specific activity toward Avicel. The activity of the isolated enzyme was optimum at a pH of 5.0 and temperature of 40 degrees C, respectively. The isoelectric point of the enzyme was 4.3. T(m) was found to be 57 degrees C. The treatment of the endoglucanase with diethylpyrocarbonate resulted in the modification of the histidine residues present in the enzyme, with a concomitant loss of 70% of the original enzymatic activity. However, carbodiimide completely inactivated the endoglucanase. The results show that the enzyme is able to sustain 50% of its activity even when heated at 90 degrees C for a period of 5 h. Endoglucanase can be used in the controlled hydrolysis of cellulose and other cellulose-rich foods. It can be used in the development of targeted functional foods from agrimaterials for value addition in the food chain.  相似文献   

Anthropogenic disturbances and status of forest and wildlife in the dry deciduous forests of Chhattisgarh state in India     

Chandra Prakash Kala  Yogesh Dubey 《林业研究》2012,23(1):45-52

The advent of modern forces and the changes in socioeconomic patterns of forest dwellers have increased the pressures on the forests. In order to mitigate such pressures and also to protect the forests and wildlife the model of protected areas networks has shifted and enhanced such pressures in the unprotected natural forests due to several reasons. Being a low profile category of protected status and continuous human settlements, the present study highlights the case of dry deciduous forests of Sarguja district of Chhattisgarh state of India. The major objectives of this study were to quantify the status of forests and wildlife and also to determine the extent of anthropogenic disturbances faced by the dry deciduous forests of central India. Transect and silent drive count methods were used for sampling wildlife and quadrat method was used for sampling vegetation. Besides, the local uses of various forest produces were also studied in view of understanding the people dependency on forests. The forest vegetation, in the study area, was pre-dominated by Shorea robusta, which had Madhuca indica, Diospyrus melanoxylon and Buchnania lanzan as the major companion species. The forest had either the high girth class mature tree species or the saplings. The low vegetation cover and density were due to the high anthropogenic pressures mainly in the form of heavy livestock grazing and collection of ethnobotanically important species. The study though reveals that the area is not rich in wildlife and the forest is fragmented, the area still supports some important species, which include many rare and endangered plants and animals. The findings of this study have been discussed in view of the management and conservation of the forest and wildlife in the dry deciduous forests.  相似文献   

Financial feasibility of increasing carbon sequestration in harvested wood products in Mississippi     

Prakash Nepal  Robert K. GralaDonald L. Grebner 《Forest Policy and Economics》2012,14(1):99-106

Longer forest rotation ages can potentially increase accumulation of carbon in harvested wood products due to a larger proportion of sawlogs that can be used for manufacturing durable wood products such as lumber and plywood. This study quantified amounts of carbon accumulated in wood products harvested from loblolly pine (Pinus taeda L.) stands grown in Mississippi by extending rotation ages traditionally used to manage these stands for timber. The financial viability of this approach was examined based on carbon payments received by landowners for sequestering carbon in standing trees and harvested wood products. Results indicated a potential to increase carbon accumulated in wood products by 16.11 metric tons (t) of carbon dioxide equivalent (CO₂e) per hectare (ha) for a rotation increase of 5 years and 67.07 tCO₂e/ha for a rotation increase of 65 years. Carbon prices of $50/tCO₂e and $110/tCO₂e would be required to provide a sufficient incentive to forest landowners to extend rotations by 5 and 10 years, respectively. With 2.8 million ha of loblolly pine stands in Mississippi, this translates to a possible increase in wood products carbon of 45 million tCO₂e and 80 million tCO₂e for harvest ages increased by 5 and 10 years, respectively. Higher carbon prices lengthened rotation ages modestly due to low present values of carbon accumulated with long rotations.  相似文献   

Farm-scale zoning of extreme temperatures in Southern Mallee,Victoria, Australia     

Prakash N. Dixit  Deli Chen 《Biosystems Engineering》2010,105(2):198-204

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Effects of long-term growth hormone-releasing factor treatment on growth, feed conversion efficiency and dry matter intake in growing female buffaloes (Bubalus bubalis)     

Mondal M  Prakash BS 《Journal of animal physiology and animal nutrition》2005,89(7-8):260-267

Effects of long-term growth hormone-releasing factor (GRF) on growth performance, feed conversion efficiency (FCE) and dry matter intake (DMI) were studied in growing buffaloes. Twelve female Murrah buffaloes of 6-8 months of age were divided into two groups of six each on the basis of their body weights so that the average body weights of the groups did not differ (p > 0.05). Animals of each group were administered intravenously with either sterile distilled water (control group) or equal volume of GRF solution containing 10 mug GRF (1-44)-NH2/100 kg body weight (treatment group) at fortnight interval from week 6 (5-week pre-treatment period) for 36 weeks (weeks 6-42 treatment period). Thereafter a 10-week post-treatment period was added. All the animals were weighed consecutively 2 days in a week and the average body weight of the two observations in the week was thus considered for further calculation. Dry matter intake was recorded daily. Average daily gain, FCE and DMI/100 kg body weight were also calculated. Plasma progesterone was estimated in the samples collected twice a week at 3-4-day intervals to assess whether either group had begun ovarian cycles. It was found that ADG and FCE were higher (p < 0.01) in GRF-treated animals during treatment and even 10-week post-treatment period. Interestingly, total DMI was not different (p > 0.05) between the groups during treatment period but found to be lower in GRF treated animals during post-treatment period. The DMI/100 kg body weight was lower (p < 0.01) in GRF-treated animals during treatment and even after cessation of treatment for 10 weeks. The GRF administration for long-term increased (p < 0.05) plasma progesterone. Plasma progesterone concentrations suggest that no animal from either group reached puberty till the end of the experiment. In conclusion, repeated GRF administration for longer term decreased (p < 0.01) DMI/100 kg body weight and increased (p < 0.01) FCE and enabled the animals to grow faster.  相似文献   

Effects of Long-term Growth Hormone-releasing Factor Administration on Plasma Growth Hormone, Luteinizing Hormone and Progesterone Profiles in Growing Female Buffaloes (Bubalus bubalis)     

M Mondal  BS Prakash 《Reproduction in domestic animals》2004,39(5):333-339

To investigate the effects of long-term growth hormone-releasing factor (GRF) administration on plasma growth hormone (GH), LH and progesterone and body weight gain in growing buffalo calves, 12 female Murrah buffaloes within the age group of 6-8 months of age were divided into two groups (treatment and control groups) of six each in such a way so that average body weights between the groups did not differ (p > 0.05). Control buffaloes were not given any hormonal treatment and treatment group buffaloes were treated with synthetic bovine GRF [bGRF (1-44)-NH(2)] at the rate of 10 microg/100 kg body weight intravenously at an interval of 15 days from week 6 (5-week pre-treatment period) till 18 injections were completed (week 6-42 treatment period) and thereafter, effect of exogenous GRF were observed for 10-week post-treatment period. Jugular blood samples were drawn twice a week at 3-4-day intervals for plasma GH, LH and progesterone quantification. Body weight of all animals was recorded twice a week. During pre-treatment period, mean plasma GH, LH and progesterone did not differ (p > 0.05) between the groups. But during treatment as well as post-treatment period, mean plasma GH levels were found to be significantly (p < 0.01) higher in treatment than control group of buffaloes. Administration of GRF for longer term sustained a higher level of plasma GH even after cessation of treatment. GRF-treated buffaloes attained higher (p < 0.01) body weight than the controls. Repeated GRF administration for long-term significantly (p < 0.01) increased plasma LH and progesterone. In conclusion, repeated long-term exogenous GRF administration induces and even enhances GH release without any sign of refractoriness. GRF may, therefore, be used to induce daily GH release without loss of responsiveness over an extended period of time in young growing female buffaloes and it may assist these animals to grow faster.  相似文献   

Effect of feeding Lagerstroemia speciosa and conventional fodder based rations on nutrient utilization,ruminal metabolites and body weight gain in mithun (Bos frontalis)     

B. Prakash  A. Dhali  M. Mondal  M. Sangtam  K. Khate  S. S. Rathore  C. Rajkhowa 《Journal of animal physiology and animal nutrition》2008,92(5):591-596

The aim of the present study was to evaluate the effect of feeding green fodder, rice straw and concentrate‐based total mixed rations (TMR) on dry matter (DM) intake (DMI), nutrient utilization, rumen fermentation patterns and body weight (BW) gain (BWG) in mithun (Bos frontalis) calves. In a randomized block design, male mithun calves (n = 18, 8–10 months of age, 121 ± 2 kg BW) were randomly divided into three experimental equal groups (six animals in each group) and fed isonitrogenous TMRs ad libitum for 120 days. The TMR₁ contained 30% Napier grass and 30% rice straw, TMR₂ contained 60% rice straw and TMR₃ contained 30% tree leaves (Lagerstroemia speciosa) and 30% rice straw (DM basis). All the TMRs contained 40% concentrate mixture (DM basis). The results indicated that the BWG, DMI and feed conversion efficiency were significantly (p < 0.01) increased with the inclusion of green fodder in TMRs. The apparent digestibility of DM, crude protein, ether extract, crude fibre and nitrogen free extract were also improved significantly (p < 0.01) with the inclusion of green fodder in TMRs. The higher concentration of total nitrogen and total volatile fatty acid in rumen liquor, but low ruminal pH were evident in animals fed green fodder supplemented TMRs. An increased (p < 0.01) molar proportion of acetic acid was evident in animals fed rice straw‐based TMR. In contrast, the molar proportion of propionic and butyric acids were significantly (p < 0.01) higher in animals fed green fodder supplemented TMRs. On the basis of higher DMI and higher daily BWG, it is concluded that Napier grass and L. speciosa tree leaves may be incorporated upto 30% (DM basis) in TMR of growing mithuns for feeding in complete confinement system.  相似文献