Piscine reovirus,but not Jaundice Syndrome,was transmissible to Chinook Salmon,Oncorhynchus tshawytscha (Walbaum), Sockeye Salmon,Oncorhynchus nerka (Walbaum), and Atlantic Salmon,Salmo salar L.          下载免费PDF全文

K A Garver  G D Marty  S N Cockburn  J Richard  L M Hawley  A Müller  R L Thompson  M K Purcell  S Saksida 《Journal of fish diseases》2016,39(2):117-128

A Jaundice Syndrome occurs sporadically among sea‐pen‐farmed Chinook Salmon in British Columbia, the westernmost province of Canada. Affected salmon are easily identified by a distinctive yellow discolouration of the abdominal and periorbital regions. Through traditional diagnostics, no bacterial or viral agents were cultured from tissues of jaundiced Chinook Salmon; however, piscine reovirus (PRV) was identified via RT‐rPCR in all 10 affected fish sampled. By histopathology, Jaundice Syndrome is an acute to peracute systemic disease, and the time from first clinical signs to death is likely <48 h; renal tubular epithelial cell necrosis is the most consistent lesion. In an infectivity trial, Chinook Salmon, Sockeye Salmon and Atlantic Salmon, intraperitoneally inoculated with a PRV‐positive organ homogenate from jaundiced Chinook Salmon, developed no gross or microscopic evidence of jaundice despite persistence of PRV for the 5‐month holding period. The results from this study demonstrate that the Jaundice Syndrome was not transmissible by injection of material from infected fish and that PRV was not the sole aetiological factor for the condition. Additionally, these findings showed the Pacific coast strain of PRV, while transmissible, was of low pathogenicity for Atlantic Salmon, Chinook Salmon and Sockeye Salmon.  相似文献   

Atlantic salmon,Salmo salar L. are broadly susceptible to isolates representing the North American genogroups of infectious hematopoietic necrosis virus          下载免费PDF全文

G Kurath  J R Winton  O B Dale  M K Purcell  K Falk  R A Busch 《Journal of fish diseases》2016,39(1):55-67

Beginning in 1992, three epidemic waves of infectious hematopoietic necrosis, often with high mortality, occurred in farmed Atlantic salmon Salmo salar L. on the west coast of North America. We compared the virulence of eleven strains of infectious hematopoietic necrosis virus (IHNV), representing the U, M and L genogroups, in experimental challenges of juvenile Atlantic salmon in freshwater. All strains caused mortality and there was wide variation within genogroups: cumulative mortality for five U‐group strains ranged from 20 to 100%, four M‐group strains ranged 30‐63% and two L‐group strains varied from 41 to 81%. Thus, unlike Pacific salmonids, there was no apparent correlation of virulence in a particular host species with virus genogroup. The mortality patterns indicated two different phenotypes in terms of kinetics of disease progression and final per cent mortality, with nine strains having moderate virulence and two strains (from the U and L genogroups) having high virulence. These phenotypes were investigated by histopathology and immunohistochemistry to describe the variation in the course of IHNV disease in Atlantic salmon. The results from this study demonstrate that IHNV may become a major threat to farmed Atlantic salmon in other regions of the world where the virus has been, or may be, introduced.  相似文献   

Resting structures and resting habitat of fishers in the southern Sierra Nevada,California     

Kathryn L. Purcell  Amie K. Mazzoni  Sylvia R. Mori  Brian B. Boroski 《Forest Ecology and Management》2009

The fisher (Martes pennanti) is a forest mustelid endemic to North America that has experienced range reductions in Pacific states that have led to their listing under the Endangered Species Act as warranted but precluded by higher priorities. The viability of the southern Sierra Nevada fisher population is of particular concern due to its reduced historical range, isolated nature, and low genetic variability. We located resting structures of radio-collared fishers in the southern Sierra Nevada and compared resting and available habitat to examine selection for specific features of resting sites. Resting structures provide protection from predators and unfavorable weather and are believed to be the most limiting habitat element across fisher home ranges. Resting structures were found primarily in live trees (76%) and snags (15%). Trees used by fishers for resting were among the largest available and frequently had mistletoe infestations. Ponderosa pines (Pinus ponderosa) were used more often than expected and incense cedars (Calocedrus decurrens) less than expected. Snags were also large and in fairly advanced stages of decay. Habitat at fisher resting sites had higher canopy cover, greater basal area of snags and hardwoods, and smaller and more variable tree sizes compared to random sites. Resting sites were also found on steeper slopes and closer to streams. Canopy cover was consistently the most important variable distinguishing rest and random sites. In western North America, fishers are generally associated with late-successional forests, but changes in these forests due to logging and fire suppression have resulted in a transition to forest stands characterized by fewer large trees and more small stems. These conditions are consistent with our finding that the large rest structures were surrounded by smaller than average trees. Management practices that support the growth and retention of greater numbers of large trees and snags, while maintaining a minimum of 61% (based on moosehorn) or 56% (generated via Forest Vegetation Simulator) canopy cover and a complex horizontal and vertical forest structure, can improve and provide for future fisher habitat.  相似文献   

Effective fluorochrome marking of juvenile sea cucumbers for sea ranching and restocking     

Steven W. Purcell  Bernard F. Blockmans 《Aquaculture (Amsterdam, Netherlands)》2009,296(3-4):263-270

Dermal spicules (or ‘ossicles’) of cultured sea cucumbers can be fluorescently marked with tetracycline and calcein for sea ranching and restocking but optimal immersion conditions are unknown. Lethal and non-lethal effects, and the efficacy of marking spicules in juvenile sandfish (Holothuria scabra), were examined under different immersion conditions. Fluorescence brightness and the proportion of marked spicules generally increased with concentration and duration of immersion. Frequency of burial (an indicator of stress) in sandfish increased with both fluorochromes at concentrations above 50 mg L^− 1. Growth in the two weeks post-marking was unaffected at immersion concentrations of 50 and 100 mg L^− 1 compared to controls, but appeared inhibited by immersion in solutions of 200 and 400 mg L^− 1 of tetracycline or calcein. Sequential marking by tetracycline (yellow) and calcein (green), in either order, showed that calcein was deposited in a higher proportion of spicules. Three other fluorochromes with disparate colors, alizarin complexone, calcein blue and xylenol orange, also marked sandfish spicules and expanded the variety of dichromic combinations. Both tetracycline and calcein fluoresced more brightly when juveniles were marked at 26 or 30 °C than at 21 °C, and this low temperature appears also to reduce the proportion of spicules marked by tetracycline. Our findings show that seawater temperature should be regulated for ex situ immersion marking. The behavioral and biological sensitivities of sandfish demand care in administering the fluorochromes. Fluorochrome immersion at 100 mg L^− 1 for 24 h at ≥26 °C provides a practical compromise between minimizing the fitness of released juveniles and ensuring the efficacy of the markers for studies on the growth and survival of sea cucumbers stocked in the wild.  相似文献   

In Vitro Cytochrome P450 2E1 and 2A Activities in the Presence of Testicular Steroids     

MK Rasmussen  G Zamaratskaia  B Ekstrand 《Reproduction in domestic animals》2011,46(1):149-154

Cytochrome P450 2E1 (CYP2E1) and 2A (CYP2A) are the main enzymes involved in the metabolism of skatole in pigs. In this study, physiological concentrations of androstenone, 17β‐oestradiol and testosterone were tested for their ability to regulate CYP2E1 and CYP2A activity in liver microsomes isolated from entire male and female pigs as well as in microsomes from Saccharomyces cerevisiae expressing either human recombinant CYP2E1 or CYP2A6. We found that physiological concentrations of androstenone and oestradiol had the ability to inhibit CYP2E1 activity. The magnitude of this inhibition (approximately 30%) was similar in recombinant human CYP2E1 and microsomes from entire male pigs. This inhibition was only seen when adding the steroid to the assay 15 min before the substrate. Interestingly, CYP2E1 activity in the microsomes from female pigs was not affected. None of the investigated steroids modified the activity of recombinant human CYP2A6. However, CYP2A activity was slightly increased in the microsomes from female pigs in the presence of oestradiol, but the magnitude of this increase was very low (below 10%) and probably irrelevant. Overall, these results indicate that physiological concentrations of androstenone and oestradiol have a potential to inhibit CYP2E1 activities in vitro, and that this inhibition is gender‐specific. Further studies are needed to investigate the biochemical mechanisms underlying those differences between the genders.  相似文献   

Anaphylaxis associated with intravenous administration of alphaxalone in a dog     

M Haworth  M McEwen  B Dixon  SL Purcell 《Australian veterinary journal》2019,97(6):197-201

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Protein and amino acids of sweet potato (Ipomoea batatas (L.) Lam.) fractions     

A E Purcell  W M Walter  F G Giesbrecht 《Journal of agricultural and food chemistry》1978,26(3):699-670

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Impact of co-infection with Lepeophtheirus salmonis and Moritella viscosa on inflammatory and immune responses of Atlantic salmon (Salmo salar)     

Laura A. Carvalho  Shona K. Whyte  Laura M. Braden  Sara L. Purcell  Anthony J. Manning  Anne Muckle  Mark D. Fast 《Journal of fish diseases》2020,43(4):459-473

This study was conducted to determine the effects of a co-infection with Moritella viscosa at different exposure levels of sea lice Lepeophtheirus salmonis in Atlantic salmon (Salmo salar). M. viscosa (1.14 × 10⁶ cfu/ml) was introduced to all experimental tanks at 10 days post-lice infection (dpLs). Mean lice counts decreased over time in both the medium lice co-infection (31.5 ± 19.0 at 7 dpLs; 16.9 ± 9.3 at 46 dpLs) and high lice co-infection (62.0 ± 10.8 at 7 dpLs; 37.6 ± 11.3 at 46 dpLs). There were significantly higher mortalities and more severe skin lesions in the high lice co-infected group compared to medium lice co-infected group or M. viscosa-only infection. Quantitative gene expression analysis detected a significant upregulation of genes in skin from the high lice co-infection group consistent with severe inflammation (il-8, mmp-9, hep, saa). Skin lesions retrieved throughout the study were positive for M. viscosa growth, but these were rarely located in regions associated with lice. These results suggest that while M. viscosa infection itself may induce skin lesion development in salmon, co-infection with high numbers of lice can enhance this impact and significantly reduce the ability of these lesions to resolve, resulting in increased mortality.  相似文献   

Soybean yield and biomass responses to cumulative transpiration: Questioning widely held beliefs     

Larry C. Purcell  Jeffrey T. Edwards  Kristofor R. Brye 《Field Crops Research》2007

Yield under drought for several crops has been established as a linear function of the cumulative water transpired during the growing season. For well-watered crops, however, there are no published data on how the duration of the cropping cycle and plant population affect the relationship between yield and transpiration. We evaluated the relationship between yield and estimated cumulative transpiration (T) or evapotranspiration (ET) for well-watered soybean (Glycine max [L.] Merr.) over a wide range of maturity groups (MG, 00–VI) and population densities (10–100 plants m⁻²) for 3 years. Daily T was estimated by determining the potential ET for a given day and multiplying this by the fraction of radiation intercepted by the crop, and a crop coefficient. Soil evaporation estimates were also made using an energy-balance approach after first subtracting the amount of radiation intercepted by the canopy. Daily values of T and ET were summed from emergence to R6. For all MG, cumulative T increased linearly with increasing population density (1.30 mm plant⁻¹ m⁻²), but predicted T at low populations (y intercept) more than quadrupled with increasing maturity, from 121 mm (MG 00) to 584 mm (MG VI). In contrast to the linear increase of yield to cumulative T for crops under drought stress, yield response to cumulative T for fully irrigated soybean differing in maturity was described well by an exponential model, predicting that 90% of the asymptotic yield would be obtained at 444 mm of T. Accounting for differences in harvest index and vapor-pressure deficit during the season among cultivars of differing maturity did not resolve the non-linear response of yield or biomass to cumulative T. These data indicate that for water-replete conditions, decreased T associated with short-season soybean need not result in decreased yield relative to full-season cultivars.  相似文献   

TREATMENT OF OVINE ANNUAL RYEGRASS TOXICITY WITH CHLORDIAZEPOXIDE     

I. S. Richards    D. S. Petterson  D. A. Purcell 《Australian veterinary journal》1979,55(6):282-283

The results of pen trials indicate that sheep affected by annual ryegrass toxicity can be treated successfully with chlordiazepoxide. In the first experiment, 87% of chlordiazepoxide-treated animals survived a lethal dose of toxic ryegrass. In the second experiment, 90% of chlordiazepoxide treated animals survived compared with a 10% recovery of control animals.  相似文献