The extent of genetic diversity among Vanilla species: Comparative results for RAPD and ISSR     

Praveen C. Verma  Debasis Chakrabarty  Satya Narayan Jena  Devesh K. Mishra  Pradhyumna K. Singh  Samir V. Sawant  Rakesh Tuli 《Industrial Crops and Products》2009,29(2-3):581-589

Vanilla is a large genus of about 110 species in the orchid family (Orchidaceae), including the species Vanilla planifolia from which commercial vanilla flavoring is derived. Since most species of vanilla are considered rare and endangered there is an urgent need to conserve them through genetic analysis and propagation/conservation studies on this crop.The present study investigated the genetic diversity among nine leafy- and leaf-less Vanilla species employing 30 decamer RAPD primers and 10 ISSR primers. The species under study were diverse and displayed a range of variability (0–66% and 0–81% for RAPD and ISSR, respectively). A total of 154 RAPD polymorphic markers (83.24%, h = 0.378) and 93 ISSR polymorphic markers (86.11%, h = 0.363) were used to generate a genetic similarity matrix followed by the cluster analysis. Specific groupings were revealed by each cluster analysis with slight variation between two different markers. Among the nine species studied, V. planifolia, Vanilla aphylla and Vanilla tahitensis revealed very low level of variation within their collections, thus indicating a narrow genetic base. The large genetic distance of Vanilla andamanica from other species suggests its different origin. A close genetic affinity was observed between the pairs V. planifolia, V. tahitensis and Vanilla albida, V. aphylla. These are the first comparative results for RAPD and ISSR reporting inter-relationship among nine cultivated, wild and hybrid Vanilla species.  相似文献   

A ROLLER BOTTLE TISSUE CULTURE SYSTEM     

Shaw DT  Kingsland LC  Brues AM 《Science (New York, N.Y.)》1940,91(2354):148-149

  相似文献   

Neuerungen der Schädlingsbekämpfung     

H. 《Journal of pest science》1940,16(6):69-71

Ohne Zusammenfassung  相似文献   

A NEW ESTROGENIC SUBSTANCE PRESENT IN THE NEUTRAL FRACTION OF HUMAN PREGNANCY URINE     

Dorfman RI 《Science (New York, N.Y.)》1940,92(2399):585-586

In addition to the well-defined phenolic estrogens in human pregnancy urine there appears to be another estrogenic substance which is a neutral non-ketonic compound.  相似文献   

Vitamins in Canine Metabolism     

H. E. Robinson  L. D. Frederick 《Canadian journal of veterinary research》1941,5(10):288-291

  相似文献   

THREE CASES OF MALE INFERTILITY     

W. L. Hindmarsh  B.V.Sc  M.R.C.V.S.  D.V.H. 《Australian veterinary journal》1941,17(1):21-23

  相似文献   

Ein starkes Auftreten des Ampferblattk?fers     

Fr. 《Journal of pest science》1942,18(11):131

Ohne Zusammenfassung  相似文献   

THE WORK OF OCTAVE HALLAUER     

Thurston RH 《Science (New York, N.Y.)》1884,4(86):306-308

  相似文献   

Qtmas über die R. bader. Forftmärter     

R. 《European Journal of Forest Research》1865,9(1):384-387

Ohne Zusammenfassung  相似文献   

A NEW METHOD OF DETECTING AND ESTIMATING SMALL AMOUNTS OF ACETONE     

Kahlenberg L 《Science (New York, N.Y.)》1925,61(1578):344-345

  相似文献