Development and standardization of a monoclonal antibody-based rapid flow-through immunoassay for the detection of Aphanomyces invadans in the field     

B Adil  K. M. Shankar  B. T. Naveen Kumar  Rajreddy Patil  Abhiman Ballyaya  K. S. Ramesh  Sathish Rama Poojary  Omkar V. Byadgi  Prabhugouda Siriyappagouder 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(4):413-419

A monoclonal antibody-based flow-through immunoassay (FTA) was developed using a nitrocellulose membrane placed on the top of adsorbent pads enclosed in a plastic cassette with a test zone at the center. The FTA could be completed within 10 min. Clear purple dots against a white background indicated the presence of Aphanomyces (A.) invadans. The FTA limit of detection was 7 µg/mL for A. invadans compared to 56 µg/mL for the immunodot. FTA and polymerase chain reaction (PCR) could detect A. invadans in fish tissue homogenates at a 10^-11 dilution compared to a 10^-8 dilution by immunodot. In fish suffering from natural cases of epizootic ulcerative syndrome (EUS) collected from Mangalore, India, FTA and PCR could detect A. invadans in 100% of the samples compared to 89.04% detected by immunodot. FTA reagents were stable and produced expected results for 4 months when stored at 4~8℃. This rapid test could serve as simple and cost-effective on-site screening tool to detect A. invadans in fish from EUS outbreak areas and in ports during the shipment of live or frozen fish.  相似文献   

Production Performance,Serum/Yolk Cholesterol and Immune Competence of White Leghorn Layers as Influenced by Dietary Supplementation with Probiotic   总被引：2，自引：0，他引：2  

Panda AK  Reddy MR  Rama Rao SV  Praharaj NK 《Tropical animal health and production》2003,35(1):85-94

An experiment was conducted to measure the influence of a dietary probiotic on the production performance, the concentrations of cholesterol in the serum and yolk and immune competence in White Leghorn layers from 25 to 72 weeks of age. One hundred and twenty commercial White Leghorn layers, aged 24 weeks, with an average of 62% hen-day egg production, were equally and randomly distributed into three groups, with eight replicates of 5 birds in each. The birds were reared in individual laying cages. They were placed on one of three dietary treatments: basal, or basal with probiotic supplementation at a rate of 100 or 200 mg/kg feed. The addition of probiotic significantly increased the egg production, shell weight, shell thickness and serum calcium, and reduced the concentrations of cholesterol in the serum and yolk. However, no differences in these traits was observed between the groups receiving 100 or 200 mg probiotic. Feed conversion, egg weight, serum phosphorus and serum alkaline phosphatase activity were not influenced by supplementation with probiotic. Antibody production in response to the inoculation of sheep red blood cells and the cutaneous basophilic hypersensitivity (CBH) responses to inoculation with phytohaemagglutinin did not differ significantly among the dietary groups at either 24 or 40 weeks of age. The antibody titre was significantly higher in the groups supplemented with probiotic at 64 weeks of age. The addition of 100 mg/kg of probiotic in the diet significantly increased the CBH response at 64 weeks of age.  相似文献   

天然生长促进剂在家禽饲养中的应用前景     

K. Panda  S.V.Rama  M.V.L. N.Raju  张晓玲  舒畅 《国外畜牧学(猪与禽)》2007,27(4):13-15

欧洲已禁止在饲料中使用抗生素生长促进剂(AGPs),将来其它许多国家也将跟随其后。因此,寻找AGPs替代品将是一项不间断的探索工作。印度家禽项目董事会的科学家断定,无论何时找到AGPs替代品,天然生长促进剂可以使家禽获益。  相似文献   

Utilisation of sesame (Sesamum indicum) seed meal in broiler chicken diets     

Rama Rao SV  Raju MV  Panda AK  Poonam NS  Shyam Sunder G  Sharma RP 《British poultry science》2008,49(1):81-85

1. Soybean meal (SBM) was replaced with sesame seed meal (SSM) at proportions of 0.00, 0.33, 0.67 and 1.00 on a protein basis in broiler diets (2 to 42 d of age). Each diet was fed to 8 replicates of 5 birds each. 2. Body weight gain, food efficiency, ready-to-cook (RTC) yield, relative weights of giblet, liver and abdominal fat were not affected by including SSM up to 0.67 proportion of SBM in starter and finisher diets (360 and 310 g/kg, respectively). The relation between level of SSM and growth performance variable (weight gain, food efficiency and RTC yield) was non-linear. 3. Weight, breaking strength and ash content of tibia decreased non-linearly with the proportion of SSM in diet. 4. The activity of alkaline phosphatase and protein concentration in serum decreased linearly with the amount of SSM in the diet.  相似文献   

Effect of supplementing germinated sprouts of pulses on performance,carcass variables,immune and oxidative stress indicators in broiler chickens reared during tropical summer season     

Rama Rao  S. V.  Prakash  B.  Rajkumar  U.  Raju  M. V. L. N.  Srilatha  T.  Reddy  E. P. K. 《Tropical animal health and production》2018,50(5):1147-1154

Tropical Animal Health and Production - An experiment was conducted to study the effects of supplementing sprouts of pulses on performance, carcass variables, immune responses, and anti-oxidant...  相似文献   

Neoplasms of ducks in Andhra Pradesh. II. A report of four intra-hepatic tumours     

J Christopher  P Rama Rao  J V Narayana  G A Sastry 《The Indian veterinary journal》1968,45(1):7-9

  相似文献   

Antioxidant and Antiglycative Potential of Ethyl Acetate Fraction of Psidium guajava Leaf Extract in Streptozotocin-Induced Diabetic Rats     

Soman S  Rauf AA  Indira M  Rajamanickam C 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(4):386-391

Hyperglycemia causes increased protein glycation and the formation of early glycation products and advanced glycation end products (AGEs) which are major factors responsible for the complications associated with diabetes. The aim of the present study was to investigate the antioxidant as well as antiglycative potential of ethyl acetate fraction of guava leaves. Oral administration of the extract at different doses showed a significant decrease in blood glucose level. It also showed an improved antioxidant potential as evidenced by decreased lipid peroxidation and a significant increase in the activity of various antioxidant enzymes such as catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase. Glycated hemoglobin as well as fructosamine which are indicators of glycation was also reduced significantly in treated groups when compared to diabetic control. In vitro studies also support the antioxidant as well as antiglycative potential of guava leaves.  相似文献   

Accumulation of Cadmium and Antioxidant and Hormonal Responses in the Indian Major Carp <Emphasis Type="Italic">Cirrhinus mrigala</Emphasis> During Acute and Sublethal Exposure     

Annamalai Malarvizhi  Manoharan Saravanan  Rama Krishnan Poopal  Jang-Hyun Hur  Mathan Ramesh 《Water, air, and soil pollution》2017,228(8):310

Acute (24 h) and sublethal (35 days) effects of cadmium chloride (CdCl₂) were examined in Cirrhinus mrigala using various endpoints (accumulation pattern, thyroid hormones (THs), and antioxidants). The mean concentrations of CdCl₂ for 24 and 96 h were found to be 35.974 and 22.387 mg L^?l, respectively. LC50 concentration of CdCl₂ for 24 h (35.97 mg L^?l) was used for the acute study. For the sublethal studies, fish were exposed to 3.59 mg L^?1 (Treatment I) and 7.19 mg L^?1 (Treatment II) corresponding to 1/10th and 1/5th of 24 h LC50 of the CdCl₂. During acute exposure, higher accumulation of CdCl₂ was noticed in the gill, liver, and kidney of C. mrigala, which is found in the order gill > liver > kidney tissues. Similarly, in sublethal treatments (Treatment I and II), a concentration and time-dependent increase of CdCl₂ accumulation was noticed in the order of gill > liver > kidney. GSH, GST, and GPx activities were found to be relatively lower from the treated groups in both acute and sublethal treatments. However, LPO activity was significantly increased in CdCl₂-treated fish C. mrigala. Further, plasma T₃ reduction was more pronounced than T₄ in acute study. During sublethal treatments, both T₄ and T₃ levels showed a continuous decrease as the exposure period extended. All the values in this study were statically significant (P < 0.01 and P < 0.05).  相似文献   

Effect of dietary inclusion of graded levels of toasted guar meal on performance,nutrient digestibility,carcass traits,and serum parameters in commercial broiler chickens     

E. Tirupathi Reddy  V. Ravinder Reddy  V. Chinni Preetham  S.V. Rama Rao  D. Srinivasa Rao 《Tropical animal health and production》2017,49(7):1409-1414

An experiment was conducted in a completely randomized design to explore the replacement value of toasted guar meal (TGM) for soybean meal (SBM) in commercial broiler diets. Hypothesis was tested by including graded levels (0, 6, 9, 12, 15, and 18% of diet) of TGM to replace maize-SBM on growth performance, apparent nutrient digestibility, carcass traits, and serum parameters. A total of six iso-nitrogenous and iso-caloric diets were prepared, and each diet was fed ad libitum to 12 replicates of five chicks each from 1 to 42 days of age. Results showed that inclusion of TGM up to 12% in broiler diets did not affect the body weight gain, feed efficiency, and energy digestibility. Feed intake, dry matter, nitrogen digestibility, and relative weights of ready-to-cook yields, breast muscle, abdominal fat, liver, and pancreas were not affected (P > 0.05) by incorporating TGM even up to 18% in broiler diets. Concentration of glucose, total protein, and triglyceride in serum was also not affected (P > 0.05), while serum total cholesterol concentration was found to be higher (P < 0.05) in broilers fed diets containing TGM as compared to those fed on 0% TGM diet. From the results, it was evident that TGM may be incorporated up to 12% in commercial broiler diets for better growth performance, nutrient digestibility, and carcass traits.  相似文献   

Influence of conservation measures on groundwater regime in a semi arid tract of South India     

M.S. Rama Mohan Rao  R.N. Adhikari  S. Chittaranjan  M. Chandrappa 《Agricultural Water Management》1996,30(3):301-312

A study was conducted in peninsular India in a predominantly agricultural watershed treated with soil and water conservation measures such as diversion drains and staggered contour trenches in nonarable land, terraces of trapezoidal cross section with graded channel on the upstream side (locally termed as graded bund) and stone checks in arable lands and rockfill dams, archweir and nala bund (a local term used for earthen embankment across the drainage channel) across the gully. Hydrological analysis has revealed that integrated management of land and water resources has consistently improved the groundwater regime. Surface runoff from the treated forest and agricultural catchment were only 27.4 and 57.4% of the untreated agricultural catchment, reflecting in high infiltration of rain water due to enhanced opportunity time. Consequently, water levels in the open wells rose by 0.5 to 1.0 m, thereby increasing the area irrigated by the wells by 172% when compared to the preproject period, which in turn improved crop yields by 70%. Hypssometric analysis indicated that water surface levels do not follow the trend of land surface levels due to the nature of the underground geological formation.  相似文献