Successful rearing of common octopus (Octopus vulgaris) fed a formulated feed in an offshore cage     

Jesús Cerezo Valverde  Tania Rodríguez‐Gonzlez  María Dolores Granero‐Fernndez  Felipe Aguado‐Gimnez  Benjamín García García 《Aquaculture Research》2019,50(3):968-972

  相似文献   

Effects of an Enterococcus faecium‐based probiotic on growth performance and health of Pirarucu,Arapaima gigas     

Natalino da Costa Sousa  Mrcia Valria Silva do Couto  Higo Andrade Abe  Peterson Emmanuel Guimares Paixo  Carlos Alberto Martins Cordeiro  Emilly Monteiro Lopes  Jonathan Stuart Ready  Gabriel Fernandes Alves Jesus  Mauricio Latera Martins  Jos Luiz Pereira Mourio  Paulo Csar Falanghe Carneiro  Alexandre Nizio Maria  Rodrigo Yudi Fujimoto 《Aquaculture Research》2019,50(12):3720-3728

Intensive fish farming has resulted in an increased concern for disease outbreaks. Probiotic use is one of the strategies being developed to improve fish health and productivity. Measures of probiotic colonization, growth performance, haematological characteristics and parasite load were used to evaluate the effect of diets supplemented with Enterococcus faecium on growth and health of Arapaima gigas juveniles. A completely randomized design with four treatments (diet with E. faecium at 1 × 10⁶ CFU/g and 1 × 10⁸ CFU/g, control diet and diet with the culture medium MRS) and three replicates was used. Ninety‐six Arapaima juveniles were distributed in 12 cages fed with the specified diet for 68 days. Colonization of the intestinal tract by lactic acid bacteria reduced the total number of heterotrophic bacteria in fish fed with probiotics compared to controls. Fish fed a supplemented diet containing 1 × 10⁸ CFU/g presented higher values of weight gain, survival and fish growth uniformity, and lower values of feed conversion ratio. The prevalence of Trichodina sp. could have affected the survival of fish in the control group. Reduction in parasite load and an increase in haematocrit, the number of erythrocytes, thrombocytes, neutrophils and monocytes were also observed in fish fed the diet containing 1 × 10⁸ CFU/g. Enterococcus faecium presented a probiotic effect in A. gigas juveniles and can be recommended for use at a concentration of 1 × 10⁸ CFU/g to modify the gut microbiota, improve growth performance and haematology and reduce parasitic load.  相似文献   

Leukocyte response and phagocytic activity in Nile tilapia experimentally infected with <Emphasis Type="Italic">Enterococcus</Emphasis> sp.     

Martins ML  Vieira FN  Jerônimo GT  Mouriño JL  Dotta G  Speck GM  Bezerra AJ  Pedrotti FS  Buglione-Neto CC  Pereira G 《Fish physiology and biochemistry》2009,35(1):219-222

This study evaluated the total and differential leukocyte counting and the phagocytic activity in Nile tilapia Oreochromis niloticus experimentally injected with Enterococcus sp. in the swim bladder. Fish were distributed in four treatments in triplicates of non-injected fish, fish injected with 1 ml of sterile saline solution 0.65%, and fish injected with 1 × 10³ and 1 × 10⁶ colony-forming units (CFU) of Enterococcus diluted in 1 ml sterile saline. Twenty-four hours after injection, the fish were anesthetized and the blood collected for white blood cell (WBC) counts, differential counting of WBC, and phagocytic activity of blood leukocytes. The increased numbers of WBC and lymphocytes were followed by decreased number of monocyte after infection. The percentages of phagocytic activities in the blood were 55.3 and 55.9%, respectively, in tilapia injected with 1 × 10³ and 1 × 10⁶ CFU/ml.  相似文献   

Remarks on genetic diversity and relationship of <Emphasis Type="Italic">Punica protopunica</Emphasis> and <Emphasis Type="Italic">P. granatum</Emphasis> assessed by molecular analyses     

Muhammad Youssef  Arif Saeed Alhammadi  Jorge Humberto Ramírez-Prado  Lorenzo Felipe Sánchez-Teyer  Rosa María Escobedo-GraciaMedrano 《Genetic Resources and Crop Evolution》2018,65(2):577-590

Here, two Punica species, viz., P. protopunica Balf. fil., reported as native to Socotra, and P. granatum L., were compared for the first time. Analysis of one P. protopunica and eleven P. granatum accessions was performed using three molecular markers, i.e., sequence related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP), and intron targeted amplified polymorphism (ITAP), along with analysis of pgWD40 sequences, a gene involved in anthocyanin biosynthesis. All markers revealed the relationship between the two species and placed them at 33% similarity. SRAP, TRAP, and ITAP generated a total of 299, 260, and 160 bands, respectively. Of these, 78, 74, and 41 bands were specific for P. protopunica, and 92, 85, and 57 bands, respectively, were shared between both species. Sequence analysis of pgWD40~870 bp amplicons exhibited 100% identity among P. granatum accessions and 98% identity to that of P. protopunica. Phylogenetic analysis of WD40 sequences from monocot and dicot species, including both Punica species confirmed the relation between P. protopunica and P. granatum, supporting earlier reports that P. protopunica could be an ancestral species of P. granatum. Furthermore, the genetic diversity among and within P. granatum accessions from Egypt (3), Mexico (5), and Yemen (3) was assessed. Molecular marker-based relationships among region-bulked accessions was approximately the same (~90% similarity), whereas the degree of genetic variation was altered within each region. Specific bands (alleles) for accessions of each region along with those shared among them were identified. Thus, these bands could be used for pomegranate genotyping and breeding programs.  相似文献   

Combining ability of common bean parents in different seasons,locations and generations     

Lisandra Magna Moura  Rafael Silva Ramos dos Anjos  Renata Oliveira Batista  Naine Martins do Vale  Cosme Damião Cruz  José Eustáquio de Souza Carneiro  Juarez Campolina Machado  Pedro Crescêncio Souza Carneiro 《Euphytica》2018,214(10):181

The objectives of this study were to obtain information about genetic parameters of a partial diallel in common bean, as well as to evaluate the effects of growing seasons, locations and generations in these estimates in order to define the best strategies for the use of diallel analysis as methodology of selection of parents and populations in common bean breeding. Twelve parents were crossed in a partial diallel scheme (5?×?7), and the F₁ and F₂ generations were evaluated in four experiments (I, II, III, and IV). The effects of seasons were determined in the Experiments I and II, in which the F₁ generation was evaluated in two distinct growing seasons. To quantify the effect of locations, we used Experiments III and IV, in which the F₂ generation was evaluated in the same season, but at different locations, and to quantify the effect of generations, the experiments II (generation F₁) and III (F₂ generation) were evaluated in the same season and location. As for grain yield the genetic control varied according to the environment. An effect of interaction on the genetic parameters of the diallel was observed for generation, whereas for grain yield, effects of season and location were observed. For plant architecture, parental combining ability should therefore be estimated using the F₁ generation, while for grain yield, both the F₁ and F₂ can be used. The recombination between the best hybrids is a promising strategy for extraction of superior black bean lines.  相似文献   

Digestive enzyme activity in the intestine of Nile tilapia (<Emphasis Type="Italic">Oreochromis niloticus</Emphasis> L.) under pond and cage farming systems     

Juliana Ferreira Santos  Karollina Lopes Siqueira Soares  Caio Rodrigo Dias Assis  Carlos Augusto Martins Guerra  Daniel Lemos  Luiz Bezerra CarvalhoJr.  Ranilson Souza Bezerra 《Fish physiology and biochemistry》2016,42(5):1259-1274

The effect of different farming systems (cage, pond) upon digestive enzyme activities of Nile tilapia was evaluated. Juvenile Nile tilapia (87.61 ± 1.52 g) were simultaneously cultured in pond and cage systems during 90 days. Cages used nutritional biphasic plan (35 and 32 % crude protein—CP feeds) and ponds used nutritional triphasic plan (35, 32 and 28 % CP feeds). Biometric measurements were monthly performed for adjustments in feeding regimes and removal of intestine tissues to evaluate the performance of enzyme activities. Total proteolytic, amylase and lipase activities were not statistically different between the treatments throughout the periods analyzed (31, 63 and 94 days of culture). However, trypsin and chymotrypsin activities were higher with 31 and 63 days of culture in fish from pond system, suggesting that natural food may have influenced these activities. A positive correlation was observed between the recommended concentration of essential amino acids for Nile tilapia and specific aminopeptidases activity in fish cage system. Substrate–SDS–PAGE revealed 12 active proteolytic bands in both systems. However, integrated density (ID) values were higher in the bands of ponds. Specimens of either cage or pond exhibited five bands of amylolytic activity. Fish from cage and pond systems showed the highest values of ID within 31 days of cultivation. In this study, the complexity of digestive functions could be verified for animals maintained under commercial conditions. Some of the assessed enzymes may show adaptations of their activities and/or expression that allow the fish to achieve a more efficient nutrient assimilation.  相似文献   

4‐Phenylphenalenones as a template for new photodynamic compounds against Mycosphaerella fijiensis          下载免费PDF全文

William Hidalgo  Marisol Cano  Manuela Arbelaez  Edwin Zarrazola  Jesús Gil  Bernd Schneider  Felipe Otálvaro 《Pest management science》2016,72(4):796-800

  相似文献   

Potential use of NIR spectroscopy to identify Cryptomeria japonica varieties from southern Brazil     

Silvana Nisgoski  Felipe Zatt Schardosin  Francielli Rodrigues Ribeiro Batista  Graciela Inés Bolzon de Muñiz  Mayara Elita Carneiro 《Wood Science and Technology》2016,50(1):71-80

  相似文献   

Characterization of the structure,dynamics, and productivity of mixed-species stands: review and perspectives     

Miren del Río  Hans Pretzsch  Iciar Alberdi  Kamil Bielak  Felipe Bravo  Andreas Brunner  Sonia Condés  Mark J. Ducey  Teresa Fonseca  Nikolas von Lüpke  Maciej Pach  Sanja Peric  Thomas Perot  Zahera Souidi  Peter Spathelf  Hubert Sterba  Martina Tijardovic  Margarida Tomé  Patrick Vallet  Andrés Bravo-Oviedo 《European Journal of Forest Research》2016,135(1):23-49

  相似文献   

Alternative silvicultural stand density management options for Chilimo dry afro-montane mixed natural uneven-aged forest using species proportion in Central Highlands,Ethiopia     

Mehari A. Tesfaye  Felipe Bravo  Andrés Bravo-Oviedo 《European Journal of Forest Research》2016,135(5):827-838

  相似文献