Methyl linoleate oxidation in the presence of bovine serum albumin   总被引：1，自引：0，他引：1  

Hidalgo FJ  Zamora R 《Journal of agricultural and food chemistry》2002,50(19):5463-5467

The oxidation of methyl linoleate (LMe) in the presence of bovine serum albumin (BSA) was studied to analyze both the processes involved when lipid oxidation occurs in the presence of proteins and the relative progression of the several reactions implicated. The disappearance of LMe, the formation of primary and secondary lipid oxidation products, the loss of essential amino acids, and the production of oxidized lipid/amino acid reaction products (OLAARPs) were studied as a function of incubation time. During the first steps of lipid oxidation, LMe was converted quantitatively to methyl linoleate hydroperoxides, which were very rapidly degraded to either secondary products of lipid oxidation or OLAARPs. No significant differences were identified in the major lipid oxidation products formed in incubations with or without proteins, indicating that mechanisms for formation of these compounds are similar in both cases. In addition, no significant differences were observed between the time-courses of formation of secondary oxidation products and OLAARPs, suggesting that hydroperoxide decomposition and OLAARP formation occur simultaneously when the lipid oxidation process takes place in the presence of proteins. Furthermore, OLAARP formation seems to be an unavoidable process that should be considered as a last step in the lipid peroxidation process.  相似文献   

Influence of harvest date and crop yield on the fatty acid composition of virgin olive oils from cv. Picual     

Beltrán G  Del Rio C  Sánchez S  Martínez L 《Journal of agricultural and food chemistry》2004,52(11):3434-3440

In this study was analyzed the effect of crop year and harvesting time on the fatty acid composition of cv. Picual virgin olive oil. The study was carried out during the fruit ripening period for three crop seasons. The mean fatty acid composition of Picual oils was determined. The oils contained palmitic acid (11.9%), oleic acid (79.3%), and linoleic acid (2.95%). The content of palmitic acid and saturated fatty acids decreased during fruit ripening while oleic and linoleic acids increased. The amount of stearic and linolenic acids decreased. The amount of saturated acids, palmitic and stearic, and the polyunsaturated acids linoleic and linolenic was dependent on the time of harvest, whereas the amount of oleic acid varied with the crop year. The differences observed between crop years for both palmitic and linoleic acid may be explained by the differences in the temperature during oil biosynthesis and by the amount of summer rainfall for oleic acid content. A significant relationship was observed between the MUFA/PUFA ratio and the oxidative stability measured by the Rancimat method.  相似文献   

Variation and genetic diversity for gliadins in Spanish spelt wheat accessions     

L. Caballero  L.M. Martín  J.B. Alvarez 《Genetic Resources and Crop Evolution》2004,51(7):679-686

Gliadin composition has been analysed in 403 accessions of spelt wheat (Triticum aestivum ssp. spelta); 61 different patterns were found for the -gliadins, 44 for the -gliadins, 19 for the -gliadins and 15 for the -gliadins. A subset of 333 accessions belonging to fifty populations from Asturias, North of Spain, showed high levels of genetic variation (A = 3.89, P = 0.88, Ne = 3.35 and He = 0.553), indicating that 82.5% of the genetic variation was within populations, and only 18.5% among populations. Thirty-five of these populations presented more of five accessions, in this new subset the values of genetic variation were higher that those of fifty populations (A = 4.49, P = 0.91, Ne = 3.80 and He = 0.595). The genetic variation within populations was 59.7% of the total, and 40.3% among populations, which could be associated to fixation effects of some alleles by genetic drift.  相似文献   

PCR-SSCP: a simple method for the authentication of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets   总被引：1，自引：0，他引：1  

Asensio L  González I  Fernández A  Rodríguez MA  Hernández PE  García T  Martín R 《Journal of agricultural and food chemistry》2001,49(4):1720-1723

A method of DNA analysis has been developed to verify the authenticity of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets. A short fragment (208 bp) of the mitochondrial 12S rRNA gene was amplified by the polymerase chain reaction and analyzed by single-strand conformation polymorphism to get species-specific patterns of single-stranded DNA (ssDNA). DNA strands were separated by native polyacrylamide gel electrophoresis and visualized by silver staining. Discrimination among the three fish species studied was possible, because each one expressed a specific ssDNA pattern.  相似文献   

Ciliates in a waste stabilization pond system in Mexico     

Fermin Rivera  M. Rosario Sanchez  Alfonso Lugo  Pedro Ramirez  Ricardo Ortiz  Arturo Calderon 《Water, air, and soil pollution》1987,34(3):245-262

A protozoological survey was performed on a waste-stabilization pond to isolate, identify, and determine the incidence of ciliates present in these waters. The correlation between the species found and the physicochemical parameters was investigated. One hundred and eighty samples were analyzed from February 1983 to February 1984. Free-living ciliates isolated include:Carchesium polypinum Linnaeus,Vorticella microstoma Ehrenberg,Vorticella convallaria Linnaeus,Didinium balbiani Fabré-Domergue,Amphileptus claparedei Stein,Spathidium spathula Müller,Litonotus fasciola Ehrenberg,Paramecium caudatum Müller,Paramecium aurelia Ehrenberg,Tetrahymena vorax Kidder,Tetrahymena pyriformis Ehrenberg,Cyclidium glaucoma Müller,Aspidisca costata Ehrenberg, andStylonychia putrina Stokes. The performance of the pond was predominantly anaerobic. The temperature, total alkalinity, and nitrate concentration were the most variable parameters in the system studied.  相似文献   

Self‐pollination does not affect fruit set or fruit characteristics in almond (Prunus dulcis)     

Pedro J. Martínez‐García  Encarnación Ortega  Federico Dicenta 《Plant Breeding》2011,130(3):367-371

  相似文献   

Competitive and allelopathic interference between soybean crop and annual wormwood (Artemisia annua L.) under field conditions     

C.M. Morvillo  E.B. de la Fuente  A. Gil  M.A. Martínez-Ghersa  J.L. González-Andújar 《European Journal of Agronomy》2011,34(4):211-221

Annual wormwood interference on soybean crop growth and yield may result from competition and allelopathy, which are modulated by crop management. Allelochemicals released by annual wormwood (e.g. artemisinin) may affect the crop directly or indirectly through the effect on the nitrogen fixing symbiont, Bradyrhizobium japonicum. The objectives were (i) to quantify the crop response (i.e. biomass production, nodulation and yield) to weed interference and (ii) to determinate the relative change of competition and allelopathy interferences, when a sublethal dose of herbicide is applied. Two split plot field experiments with three replications were used. The experiment involved a factorial combination of five weed–crop density (soybean/annual wormwood, plants m^?2) levels: D1, pure soybean, 40/0 plants m^?2; D2, 40/2 plants m^?2; D3, 40/4 plants m^?2 and D4, 40/8 plants m^?2, and D5, pure annual wormwood, 0/8 plants m^?2, two activated carbon (allelopathy) levels: C?, with activated carbon (reduced allelopathy) and C+, non activated carbon applied (with allelopathy) and two herbicide levels: H?, untreated and H+, treated with a sub-lethal dose of glyphosate. Activated carbon to adsorb allelochemicals (with and without activated carbon) and glyphosate application (with and no herbicide) were assigned to sub-plots. Increasing weed density did not affect crop biomass at flowering, but changed nodule number and soybean yield with a different pattern depending on carbon and herbicide treatment. Relative crop yield decreased with increasing relative weed biomass. This decrease was particularly drastic when allelopathy was reduced by activated carbon and without herbicide application. The maximum yield losses of 33% in 2006 and 17% in 2007 were observed with the highest weed density (8 plants m^?2). In contrast, without carbon (high allelopathy level), soybean yield remained stable within the explored range of annual wormwood biomass, despite the fact that weed biomass at high densities (D4) was high enough to generate competition. The lack of response to increasing weed density could be related to the indirect effect of allelochemicals interacting with soil microorganisms (i.e. B. japonicum) that positively affected the nodulation (e.g. larger nodules in 2006 and increased nodules biomass due to higher number of roots in 2007 at high densities). With herbicide application, soybean yield of both carbon treatments remained stable when biomass of annual wormwood increased. This research provided strong evidence in support of the existence of positive effect of allelopathic and competitive interactions between annual wormwood and soybean crop under field conditions that may be overridden under herbicide application.  相似文献   

Reference evapotranspiration estimation without local climatic data   总被引：1，自引：0，他引：1  

Pau Mart��  Pablo Gonz��lez-Altozano  Mar��a Gasque 《Irrigation Science》2011,29(6):479-495

The Penman–Monteith equation for reference evapotranspiration (ET_o) estimation cannot be applied in many situations, because climatic records are totally or partially not available or reliable. In these cases, empirical equations that rely on few climatic variables are necessary. Nevertheless, the uncertainty associated with empirical model estimations is often high. Thus, the improvement of methods relying on few climatic inputs as well as the development of emergency estimation tools that demand no local climatic records turns into a task of great relevance. The present study describes different approaches based on multiple linear regression, simple regression and artificial neural networks (ANNs) to deal with ET_o estimation exclusively from exogenous records from secondary stations. This cross-station approach is based on a continental characterization of the study region, which enables the selection and hierarchization of the most suitable ancillary data supplier stations. This procedure is compared with different traditional and cross-station approaches, including methodologies that also consider local temperature inputs. The proposed methods are also evaluated as gap infilling procedures and compared with a simple methodology, the window averaging. The artificial neural network and the multiple linear regression approaches present very similar performance accuracies, considerably higher than simple regression and traditional temperature-based approaches. The proposed input combinations allow similar performance accuracies as ANN models relying on exogenous ET_o records and local temperature measurements. The cross-station multiple linear regression procedure is recommended due to its higher simplicity.  相似文献   

The after-hours mutant reveals a role for Fbxl3 in determining mammalian circadian period     

Godinho SI  Maywood ES  Shaw L  Tucci V  Barnard AR  Busino L  Pagano M  Kendall R  Quwailid MM  Romero MR  O'neill J  Chesham JE  Brooker D  Lalanne Z  Hastings MH  Nolan PM 《Science (New York, N.Y.)》2007,316(5826):897-900

  相似文献   

Neuroscience. Zif and the survival of memory     

Izquierdo I  Cammarota M 《Science (New York, N.Y.)》2004,304(5672):829-830

  相似文献