EPS364, a Novel Deep-Sea Bacterial Exopolysaccharide,Inhibits Liver Cancer Cell Growth and Adhesion     

Yun Wang  Ge Liu  Rui Liu  Maosheng Wei  Jinxiang Zhang  Chaomin Sun 《Marine drugs》2021,19(3)

The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.  相似文献   

紫花槭秋季叶色表达转录组初步分析     

张佳奇  赵东辉  高玉福  荣立苹  李佳霖  翁卓 《分子植物育种》2021,19(21):7075-7083

为了丰富紫花槭转录组数据,进一步开展紫花槭秋季叶片呈色机制研究.本研究以紫花槭秋季转色期三个阶段(前期,中期,后期)叶片为材料,采用高通量测序技术进行转录组初步分析.转录组数据共获得50501条Unigene,有35316条Unigene在数据库中得到注释,其中NR数据库中注释到的Unigene数量最多,共35024条,占69.4％.在注释到的物种中,紫花槭比对的Unigene与甜橙(Citrus sinensis)相似度最高,共有4290条,占12.25％.紫花槭转录组中的Unigene根据GO功能可分为生物学过程、细胞组分和分子功能3大类,共有25375条,其中生物学过程的基因最多,主要聚集于代谢过程和细胞过程等.基于Unigene库的基因结构分析,其中SSR分析共获得12711个SSR标记,占Unigene总数的36％.SSR位点共包含150种重复基元,单碱基重复所占比例最高(7184个,61.86％),四碱基重复、五碱基重复和六碱基重复所占比例较低.Unigene库中共有328239个SNP位点,发生频率为1/190 bp,SNP位点分为转换和颠换两种类型的碱基替换方式,其中碱基转换位点213787个(65.13％),碱基颠换位点114452个(34.87％),碱基转换类型发生频率高于颠换类型.6种单碱基变异中,2种转换类型A/G、C/T的发生频率分别为33.03％和32.10％;4种颠换类型中A/T发生频率最高,为11.52％;C/G发生频率最低,为5.79％.紫花槭转录组秋季叶色表达的转录组分析,可为紫花槭叶色基因调控、定向分子育种和培育彩叶新品种提供研究提供基础的数据信息.  相似文献   

Transcriptome Analysis of Myzus persicae to UV-B Stress     

Chang-Li Yang  Jian-Yu Meng  Meng-Shuang Yao  Chang-Yu Zhang 《Journal of insect science (Online)》2021,21(3)

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十里香茶离体培养及再生体系研究     

杨霏  邹瑞  张玥  杨自云  吴田 《种子》2020,(4):46-49,54

为了保护珍贵的十里香茶树资源,掌握利于十里香茶的最优组培条件,本研究对十里香茶组培过程中外植体类型、消毒时间、培养基添加物及培养条件进行了筛选。结果表明,采用轻微木质化的带腋芽茎段经升汞消毒15 min,接种后暗培养5 d,MS+0.2 mg·L^-1 NAA培养基中添加3 mg·L^-1 PVP+2000 mg·L^-1 Vc或者6 mg·L^-1 PVP+3000 mg·L^-1 AC,可在低污染率的前提下将褐化率降为10%左右,一定程度上解决了茶树组培中褐化率高的难题;采用1/8 MS+1 mg·L^-1 IBA培养基有利于外植体生根且增殖迅速。本研究建立了十里香茶的无菌苗离体培养及再生体系,可通过组培手段对其进行大量繁殖。  相似文献   

优质国审棉品种——ZHM19     

黄慧  贺云新  张志刚  蒋杰  吴佶膛  张兴平  梅正鼎 《中国棉花》2020,47(11):38-39

概述了ZHM19的选育过程、特征特性、产量、纤维品质和抗病性等特点及主要栽培技术措施。  相似文献   

豚草粗提物对杨树水泡型溃疡病的抑菌作用研究     

矫丽曼  彭儒胜  冯连荣  李晓宇  赵大根  张妍  纪纯阳 《农学学报》2020,10(9):21-24

为解决豚草提取物在林业病虫害防治中的推广与应用问题,以豚草叶片为试验材料,采用温浸-超声波联合提取法提取抑菌活性物质,并开展其对杨树水泡型溃疡病病原菌的室内外抑菌试验。室内抑菌试验表明,粗提物浓度为16 mg/mL时,抑菌率达到73.62%;林间防治试验表明,最小抑菌浓度为 40 mg/mL,抑菌率达到95.14%。通过以上抑菌试验确定豚草粗提物对杨树水泡型溃疡病有较好的抑制效果。  相似文献   

云南玉溪烟区轮作棉花的环境条件分析     

张志忠 《中国棉花》2020,47(1):44-46

根据棉花生长环境的需求，结合玉溪烟区土壤和气候观测资料，分析玉溪烟区棉花的植棉土壤条件和生长发育期光、热、水等气候条件，探讨玉溪烟区轮作棉花的可行性。结果表明：新平、元江两县烟区土壤pH略低，不建议烟棉轮作；其余各县区均符合棉花生产条件，可进行烟棉轮作。  相似文献   

Effect of chlorogenic acid on intestinal inflammation,antioxidant status,and microbial community of young hens challenged with acute heat stress     

Fang Chen  Hao Zhang  Na Zhao  Xuehai Yang  Encun Du  Shaowen Huang  Wanzheng Guo  Wei Zhang  Jintao Wei 《Animal Science Journal》2021,92(1):e13619

Heat stress in poultry is deleterious to productive performance. Chlorogenic acid (CGA) exerts antibacterial, anti-inflammatory, and antioxidant properties. This study was conducted to evaluate the effects of dietary supplemental CGA on the intestinal health and cecal microbiota composition of young hens challenged with acute heat stress. 100-day-old Hy-line brown pullets were randomly divided into four groups. The control group (C) and heat stress group (HS) received a basal diet. HS + CGA₃₀₀ group and HS + CGA₆₀₀ group received a basal diet supplemented with 300- and 600-mg/kg CGA, respectively, for 2 weeks before heat stress exposure. Pullets of HS, HS + CGA₃₀₀, and HS + CGA₆₀₀ group were exposed to 38°C for 4 h while the control group was maintained at 25°C. In this study, dietary CGA supplementation had effect on mitigate the decreased T-AOC and T-SOD activities and the increasing of IL-1β and TNFα induced by acute heat stress. Dietary supplementation with 600 mg/kg CGA had better effect on increasing the relative abundance of beneficial bacterial genera, such as Rikenellaceae RC9_gut_group, Ruminococcaceae UCG-005, and Christensenellaceae R-7_group, and deceasing bacteria genera involved in inflammation, such as Sutterella species. Therefore, CGA can ameliorate acute heat stress damage through suppressing inflammation and improved antioxidant capacity and cecal microbiota composition.  相似文献   

体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察     

孙伟  包向男  乌云高娃  王建国  李永胜  李善铎  张铁柱  王飞飞  李喜和 《中国农学通报》2020,36(20):114-118

为探究体细胞克隆荷斯坦奶牛出生后死亡的原因,对新生后死亡的克隆荷斯坦公牛和自然繁殖的荷斯坦公犊的主要组织器官进行比较。通过解剖和石蜡切片-HE(hematoxylin-eosin staining)染色技术,对主要的组织器官结构进行观察和分析。结果表明,新生后死亡的克隆荷斯坦公牛肺部结构清晰;肝脏的肝细胞明显肿大,出现脂肪轻度变性;肾小管上皮细胞出现变性;心肌的肌纤维间空隙增大;骨骼肌纤维间隙明显,空泡变性,这可能导致该克隆牛犊肌肉无力并功能不全;淋巴结皮髓质界限不分明,淋巴小结细胞较稀疏,生发中心不明显,淋巴窦细胞较少;脾脏内红细胞较少,这说明该克隆牛的造血功能可能不完善;胸腺的皮质部分和髓质部分界限不明显,嗜酸性胸腺小体不易辨认,可能发育不全。该克隆公牛免疫器官出现不同程度的发育不全现象较严重,这有可能是其出生后死亡率高的主要原因。  相似文献   

中药对肉仔鸡脂质代谢及肉品质的影响   总被引：16，自引：3，他引：13  

王俊东  李淑琴  申瑞玲  郝俊虎  徐生瑞 《中国兽医学报》2003,23(4):377-379

将120只1周龄肉仔鸡随机分成4组,每组30只,前3组(试验Ⅰ、Ⅱ、Ⅲ组)分别喂以含1%的3种不同中药组方的饲粮,第Ⅳ组为对照组,只喂基础日粮.试验期6周.结果(1)与对照组相比,Ⅰ组血清TC、LDL-C极显著下降(P＜0.01);Ⅱ组血清TC、LDL-C极显著下降(P＜0.01),TG也下降(P＜0.05),HDL-C显著升高(P＜0.01);Ⅲ组血清LDL-C极显著下降(P＜0.01),HDL-C则显著升高(P＜0.01);试验Ⅰ～Ⅲ组血清总脂都极显著下降(P＜0.01);与Ⅱ组相比,Ⅰ组血清LDL-C、Ⅲ组TC存在显著差异(P＜0.01).(2)与对照组相比,胸肌组织中,各试验组TG极显著下降(P＜0.01),Ⅱ组TC显著下降(P＜0.05);腿肌中,各组TC显著下降(P＜0.05),TG下降极显著(P＜0.01);肝脏中各组TC、TG均极显著下降(P＜0.01);与Ⅱ组相比,Ⅰ组肝TG差异极显著(P＜0.01);Ⅲ组胸肌TG、肝TG有显著差异性(P＜0.05).(3)7周龄末鸡体重与对照组相比,Ⅱ组差异极显著(P＜0.01),Ⅲ组差异显著(P＜0.05);Ⅱ组料肉比比对照组极显著下降(P＜0.01).(4)肌肉中的营养成分,Ⅰ、Ⅱ组腿肌中粗脂肪比对照组极显著下降(P＜0.01),Ⅱ组腿肌中粗蛋白含量显著升高(P＜0.01).结论中药组方能不同程度地调整鸡脂质代谢,提高生产性能和改善鸡肉品质.在3个不同中药组方中,综合效果Ⅱ方最优.  相似文献