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941.
942.
Canine bicavitary carcinomatosis with transient needle tract metastasis diagnosed by multiplex immunocytochemistry 下载免费PDF全文
A Russell Moore Emily Coffey Sarah E. Leavell Greta Krafsur Colleen Duncan Kristy Dowers Kelly S. Santangelo 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(3):495-500
A 6‐year‐old, male castrated, mixed‐breed dog was referred to the James L. Voss Veterinary Teaching Hospital at Colorado State University for bicavitary effusion. On examination, the dog was tachycardic and tachypneic with bilaterally decreased lung sounds. Thoracic and abdominal ultrasonic examination revealed pleural and peritoneal effusions, which were aspirated and submitted for fluid analysis and cytology. Both cavity fluids were classified as exudates with a large population of vacuolated mononuclear cells. Multiplex immunocytochemistry (ICC) for cytokeratin and vimentin demonstrated exclusively cytokeratin expression, indicating these cells were of epithelial origin. A full diagnostic evaluation was performed, including CBC, clinical chemistry, a pet‐side test for heartworm disease, ehrlichiosis, Lyme disease, and anaplasmosis, imaging modalities of thorax, abdomen, and heart, urinalysis, and fine‐needle aspirations of spleen, liver, and popliteal lymph nodes. The dog was diagnosed with pleural and peritoneal carcinoma with presumed carcinomatosis. A single dose of intracavitary carboplatin was administered before discharge, and over a period of 2 weeks, 5 thoracocenteses were performed. A subcutaneous mass was noted at a thoracocentesis site one week after initial presentation. Cytology of the mass was consistent with carcinoma, and neoplastic seeding of the tumor cells from the thoracocentesis was suspected. The dog was euthanized 15 days after the first visit, and a necropsy was performed. Findings were consistent with carcinomatosis secondary to anaplastic pulmonary carcinoma with transient subcutaneous seeding of neoplastic cells during routine thoracocentesis. This case demonstrates the utility of multiplex ICC in the clinical setting. 相似文献
943.
Beech J Boston R Lindborg S Russell GE 《Journal of the American Veterinary Medical Association》2007,231(3):417-426
OBJECTIVE: To compare the effect of thyrotropin-releasing hormone (TRH) administration on endogenous ACTH concentrations in healthy horses and those with pituitary pars inter-media hyperplasia and compare the test with the dexamethasone suppression test (DST). DESIGN: Prospective case series. ANIMALS: 15 horses with clinical signs of pituitary pars intermedia dysfunction (PPID), 4 horses with equivocal signs of PPID, and 29 horses without signs of PPID. PROCEDURES: ACTH concentrations prior to and after administration of TRH were measured 61 times in 48 horses. Results of the DST (cortisol response) were compared with those of the TRH test in 29 horses. Thirty-three horses (24 with no clinical signs of PPID, 5 with clinical signs of PPID, and 4 with equivocal clinical signs of PPID) were euthanized and necropsied and their pituitary glands evaluated. RESULTS: ACTH concentrations increased in all horses, but magnitude and duration of increase were significantly higher in horses with PPID. Endogenous ACTH concentrations were influenced by season. The ACTH baseline concentrations and response to TRH were not correlated with results of the DST. Results of DST were abnormal only in clinically abnormal horses or those with pars intermedia hyperplasia, but were within reference range in 17 of 26 tests in these horses. CONCLUSIONS AND CLINICAL RELEVANCE: The ACTH response to TRH is a useful test for diagnosis of pituitary gland hyperplasia, particularly in horses in which baseline ACTH concentrations are within reference range. The DST was specific but not sensitive and was inconsistent for individuals, and results often did not agree with the TRH test response. 相似文献
944.
Driskill R Russell JR Strohbehn DR Morrical DG Barnhart SK Lawrence JD 《Journal of animal science》2007,85(6):1577-1586
A winter grazing experiment was conducted to evaluate the effects of stocking rate and corn gluten feed supplementation on forage mass and composition and the BW and BCS of bred 2-yr-old cows grazing stockpiled forage during winter. Two 12.2-ha blocks containing Fawn, endophyte-free, tall fescue and red clover were each divided into 4 pastures of 2.53 or 3.54 ha. Hay was harvested from the pastures in June and August of 2003 and 2004, and N was applied at 50.5 kg/ha at the initiation of stockpiling in August. On October 22, 2003, and October 20, 2004, twenty-four 30-mo-old Angus-Simmental and Angus cows were allotted by BW and BCS to strip-graze for 147 d at 0.84 or 1.19 cow/ha. Eight similar cows were allotted to 2 dry lots and fed tall fescue-red clover hay ad libitum. Corn gluten feed was fed to cows in 2 pastures to maintain a mean BCS of 5 (9-point scale) at each stocking rate and in the dry lots (high supplementation level) or when weather prevented grazing (low supplementation level) in the remaining 2 pastures at each stocking rate. Mean concentrations of CP in yr 1 and 2 and IVDMD in yr 2 were greater (P < 0.10) in hay than stockpiled forage over the winter. At the end of grazing, cows fed hay in dry lots had greater (P < 0.05) BCS in yr 1 and greater (P < 0.10) BW in yr 2 than grazing cows. Grazing cows in the high supplementation treatment had greater (P < 0.10) BW than cows grazing at the low supplementation level in yr 1. Cows in the dry lots were fed 2,565 and 2,158 kg of hay DM/cow. Amounts of corn gluten feed supplemented to cows in yr 1 and 2 were 46 and 60 kg/ cow and did not differ (P = 0.33, yr 1; P = 0.50, yr 2) between cows fed hay or grazing stockpiled forage in either year. Estimated production costs were greater for cows in the dry lots because of hay feeding. 相似文献
945.
During an outbreak of malignant catarrhal fever in a herd of Ankole cattle in a zoological collection, two adult cows and one adult bull from a herd of 15 died or were euthanased between July and November 2004. The clinical, gross postmortem and histological findings were typical of the disease in uk native domestic cattle. The diagnosis was confirmed by serology in two animals, and by pcr in all three; the pcr provided evidence of alcelaphine herpesvirus type 1 infection in all three animals and also of ovine herpesvirus type 2 infection in one. 相似文献
946.
Photochemical air quality models provide the most defensible method for relating future air quality to changes in emission, and hence are the foundation for determining the effectiveness of proposed control strategies. However, strategies, based primarily on controlling reactive organic gas emissions, have not provided the expected benefits. This raises the question of what have been the deficiencies in previous studies utilizing these tools? Furthermore, what changes are necessary, and desired, to improve upon past efforts? The current generation of models have matured within their original frameworks to represent, relatively accurately, the important physical and chemical processes affecting pollutant dynamics in urban atmospheres. The ability to follow regional dynamics is less well demonstrated. Current regional models have a single horizontal resolution scale. Multiscale models will enable detailed treatment of urban chemistry, and also effectively follow long range transport and chemistry. Improved computational capabilities will allow more detailed chemistry and heterogeneous processes to be followed within the models. The practice of photochemical modeling will benefit greatly from recent and future intensive field studies. The advancements in both the model framework and practice will allow much more accurate evaluation of proposed control strategies, and lead to a much improved understanding of pollutant dynamics. 相似文献
947.
J.S. Russell 《Agricultural and Forest Meteorology》1984,33(2-3)
Environmental and biological periodic variables have been expressed as Fourier series, combined in vector functions and then projected onto defined planes. The projections show the interactions between the variables with time. Integrated and differentiated forms of periodic variables measured as change per unit time can also be projected. Examples using annual environmental and biological data are presented but the approach has a wide applicability to relationships of any length of period. 相似文献
948.
G E Russell 《Journal of the Association of Official Analytical Chemists》1985,68(5):896-898
The official AOAC method for rot fragments in comminuted tomato products (44.224) has been revised on the basis of Youden's ruggedness testing procedures to provide better control over the sources of variability. Two sets of ruggedness tests were carried out to evaluate the type of balance used to weigh the sample, amount of stain, staining time, sieve design, technique used to transfer stained material from beaker to sieve, washing technique used to accumulate stained material at edge of sieve, diameter of eye dropper used to transfer sample from sieve to graduated tube, number of 0.5 mL portions examined, and magnification used to examine prepared slides. A 3-way analysis of variance conducted on amount of stain, transfer technique, and washing technique showed that the transfer and washing techniques were significant areas of variability. 相似文献
949.
E. K. Malone K. M. Rassnick J. J. Wakshlag D. S. Russell R. Al‐Sarraf D. M. Ruslander C. S. Johnson D. L. Trump 《Veterinary and comparative oncology》2010,8(3):209-220
Calcitriol potentiates the effect of multiple chemotherapy agents in a variety of tumour models. In this study, we examine whether calcitriol increases chemotherapy or tyrosine kinase inhibitor in vitro cytotoxicity in canine mastocytoma C2 cells. We also evaluate the in vivo effect of DN101, a highly concentrated oral formulation of calcitriol designed specifically for cancer therapy, as a single‐agent therapy in dogs with mast cell tumours (MCTs). Calcitriol exhibits synergistic, antiproliferative activity when used in combination with CCNU, vinblastine, imatinib or toceranib in vitro. The concentrations required for 50% growth inhibition were generally two‐ to six‐fold lower when the drugs were used in combination than when used individually. High‐dose oral calcitriol induced remission in 4 of 10 dogs (one complete remission, three partial remissions), although the majority experienced toxicity, necessitating discontinuation of the trial. Further evaluation of calcitriol in combination therapy for dogs with MCTs is warranted. 相似文献
950.
Russell B 《Science (New York, N.Y.)》1917,45(1173):635-636