Molecular cloning of the canine telomerase reverse transcriptase gene and its expression in neoplastic and non-neoplastic cells     

Yazawa M  Okuda M  Kanaya N  Hong SH  Takahashi T  Ohashi E  Nakagawa T  Nishimura R  Sasaki N  Masuda K  Ohno K  Tsujimoto H 《American journal of veterinary research》2003,64(11):1395-1400

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Correlation of phytoplasma concentration in Hydrangea macrophylla with green-flowering stability     

Elly Kesumawati  Takushi Kimata  Tatsuya Uemachi  Munetaka Hosokawa  Susumu Yazawa 《Scientia Horticulturae》2006

The interaction between phytoplasma concentration and green-flowering stability was studied in hydrangea cultivars. Three green and 18 nongreen cultivars were subjected to polymerase chain reaction (PCR) analysis to determine Japanese hydrangea phyllody (JHP) phytoplasma infection. The results showed that JHP-phytoplasma was detected only in ‘Midori’ plants, which have green sepals. ‘Midori’ plants were propagated, and from 29 rooted cutting plants, they were grouped into three types on the basis of sepal color, that is, green (75.9%), blue-green (13.8%) and blue (10.3%) sepals. To clarify the variability in the sepal color of ‘Midori’ plants, JHP-phytoplasma concentration in the sepals and leaves of green-, blue-green- and blue-flowering plants was determined by PCR analysis. The semiquantitative PCR comparisons of 370 bp DNA fragments showed that the JHP-phytoplasma concentrations in green sepals were 16 times higher than that in blue-green sepals. JHP-phytoplasma could not be identified by PCR analysis in blue sepals and leaves. These results showed that JHP-phytoplasma concentration correlated with green sepal stability in ‘Midori’ plants. A histological observation of sepals showed that epidermal cells of blue and blue-green sepals had a dome shape. Otherwise, green sepals were leaflike with flat epidermal cells, and palisade parenchyma cells with numerous chloroplasts.  相似文献   

Spawning induction and seed production of Eastern little tuna,Euthynnus affinis (Cantor, 1849), in the post‐ and pre‐spawning seasons by hormonal treatment in a semi‐closed recirculation system with elevated temperature          下载免费PDF全文

Ryosuke Yazawa  Yutaka Takeuchi  Kenta Satoh  Yuri Machida  Kotaro Amezawa  Naoki Kabeya  Goro Yoshizaki 《Aquaculture Research》2017,48(7):3472-3481

We previously established a method for spawning induction in Eastern little tuna (ELT) Euthynnus affinis (Cantor, 1849) by administering a gonadotropin‐releasing hormone analog (GnRHa) during the natural spawning season in Japan (August–October). In order to establish seed production of ELT in the off‐spawning season, we first conducted three spawning induction trials by GnRHa administration from October 2011 to January 2012 using ELT broodstock (2 years old; three females and four males) maintained in a 10‐m³ tank with a semi‐closed recirculation system and static elevated temperature. Average water temperature and daily egg production in three trials lasting 11–15 days were 27.0 ± 0.09°C and 268 173 eggs (Trial 1), 27.0 ± 0.11°C and 277 9098 eggs (Trial 2), and 25.5 ± 0.39°C and 291 113 eggs (Trial 3) respectively. Mean fertilization rate and mean hatching rate were 70.4% and 60.5% (Trial 1), 83.9% and 79.6% (Trial 2), and 62.5% and 57.4% (Trial 3) respectively. We also succeeded in producing ELT larvae in the pre‐spawning season (April–July), although the quantity and quality of larvae produced were inferior to those produced in other calendar months. In trials involving periodic GnRHa administration during the off‐spawning seasons, hatched larvae were obtained in the 10‐m³ tank after six of nine administrations in the 2011–2012 off‐spawning season and in 16 of 19 administrations in the 2012–2013 off‐spawning season. The findings of this study demonstrated that hormonal treatment and thermal control could be used to extend the spawning period in ELT, potentially allowing larval production in the post‐ and pre‐spawning seasons.  相似文献   

基于NDVI数据的华北地区耕地物候空间格局   总被引：13，自引：4，他引：13  

吴文斌  杨鹏  唐华俊  周清波  张莉 《中国农业科学》2009,42(2):552-560

 【目的】探讨基于多时相遥感信息监测中国华北地区耕地种植制度和物候空间格局特征。【方法】选择NDVI时间序列数据,采用非对称性高斯函数拟合方法重建平滑曲线,依据年内NDVI变化曲线峰值数目监测华北地区耕地的多熟种植制度,并利用动态阈值法获取该种植制度下耕地物候空间格局。【结果】华北地区耕地种植制度以一年两熟为主,其分布具有明显的空间差异性,随着纬度递减呈现出从简单到复杂的总体趋势。在该种植制度下,华北地区耕地第一季作物的生长开始期和生长结束期存在十分明显的空间差异,一年两熟区域的第一季作物生长开始期和生长结束期要明显早于一熟区域。与第一季作物物候期明显的空间差异相比,华北地区耕地第二季作物物候期差异不显著。【结论】华北地区耕地种植制度与物候分布格局和自然地理环境紧密相关,不同区域的温度、降水和光照等气候资源的禀赋和匹配程度对该区域的种植结构和作物布局有很大影响。此外,这种耕地物候空间格局还与作物品质、耕作水平、灌溉、施肥和农药等有密切关系。如何区别生态环境因子和人类活动因子对耕地物候的影响是今后值得深入研究的问题。  相似文献   

Pathogenicity of serotype 1 fowl adenovirus in commercial broiler chickens.     

Y Okuda  M Ono  S Yazawa  Y Imai  I Shibata  S Sato 《Avian diseases》2001,45(4):819-827

The pathogenicity of a serotype 1 fowl adenovirus (FAV-99ZH), isolated from broiler chickens exhibiting gizzard erosion, was investigated in commercial broiler chickens. Five-, 3-, and 1-wk-old commercial broiler chickens were inoculated with FAV-99ZH by both oral and ocular routes. In the 5-wk-old chickens (trial 1), none of which had the maternal antibody to FAV-99ZH, severe gizzard erosions were observed on days 5, 7, and 10 postinoculation (PI). Among the 3-wk-old chickens (trial 2), which were separated into a control group and three treatment groups according to their maternal antibody titer levels, some chickens showed clinical signs such as depression and anorexia. Compared with the control group, all the treatment groups showed decreased weight gain. One treatment group, moreover, showed significantly decreased (P < 0.05) weight gain on day 10 PI. Severe gizzard lesions, such as erosion or ulcers, were observed from day 4 PI in all treatment groups regardless of their maternal antibody levels. The 1-wk-old chickens (trial 3) were separated into a control group and two treatment groups according to their titer levels of the inoculated virus. In spite of high maternal antibody levels, severe gizzard lesions were observed in both treatment groups, which also showed decreased weight gain. One treatment group, inoculated with the higher dose, showed significantly decreased (P < 0.05) weight gain on days 10 and 14 PI compared with the control group. Fowl adenovirus was recovered mainly from gizzard and rectal (including feces) samples from inoculated chickens but was not recovered from liver samples in any of the trials or in any of the control chickens. Although the reproduced disease was similar to that described in a previous report of experimental infection of specific-pathogen-free (SPF) white leghorn chickens with fowl adenovirus, the pathogenicity of FAV-99ZH in commercial broiler chickens was more severe than that in the SPF white leghorn chickens. The results of the present study indicate that FAV-99ZH isolated from gizzard erosion had pathogenicity and produced severe lesions in the gizzards of broiler chickens and that FAV-99ZH could infect and produce illness in broiler chickens with maternal antibodies against this virus.  相似文献   

Contraceptive effect of a gonadotropin-releasing hormone vaccine on a captive female African Lion (Panthera leo): a case study     

Keisuke KAWASE  Jumpei TOMIYASU  Kazuyuki BAN  Ryosuke ONO  Satoshi ANDO  Aya ONO  Ran KIMURA  Kanako TOMISAWA  Motozumi MATSUI  Shun-ichi SHIIHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(9):1454

Lions (Panthera leo) breed well under captivity, so contraception has been commonly conducted for population management, leading to a demand for a less invasive and reversible contraceptive approach in lions. In this study, we examined the efficacy of a commercial gonadotropin-releasing hormone vaccine as a method of suppressing reproductive activity in a sexually matured female lion. Under behavioral restraint, the vaccine was injected twice (days 0 and 109). After the initial vaccination, ovarian activity is still observed. After the second vaccination, contraceptive effect was confirmed for 246 days until restart of estrous cycles. We confirmed only a slight swelling around the injection site after the second vaccination. This study may suggest an alternative option for a contraceptive method in lions.  相似文献   

Effects of components of the leaf area distribution on drag relations for Cryptomeria japonica and Chamaecyparis obtusa     

Kenji Kitagawa  Shintaro Iwama  Sho Fukui  Yuuki Sunaoka  Hayato Yazawa  Atsushi Usami  Masaaki Naramoto  Takanori Uchida  Satoshi Saito  Hiromi Mizunaga 《European Journal of Forest Research》2015,134(3):403-414

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Changes in the composition of the extracellular matrix accumulated by mesenchymal stem cells during in vitro expansion     

Ryosuke Nakamura  Fumio Nakamura  Shigeharu Fukunaga 《Animal Science Journal》2014,85(6):706-713

One of the approaches to preserve the properties of mesenchymal stem cells (MSCs) during in vitro expansion is to use cell culture substrates. MSCs are known to generate the extracellular matrix (ECM) proper to preserve their proliferative capacity in vitro, but extensive expansion is considered to deprive MSCs of the capacity to prepare such ECM. In order to examine the features of ECM proper that is required to preserve the proliferative capacity of MSCs, we analyzed the changes in the composition of ECM accumulated by MSCs during in vitro expansion. Biochemical and immunological analysis showed that collagen and laminin content decreased during expansion. Immunofluorescence and ultrastructural analyses showed that the ECM structure changed from a dynamic fibrous, porous and steric structure to a static, crammed, and planar one. The results of Western blotting analysis suggested loose intermolecular association in ECM molecules accumulated by extensively proliferated MSCs. The ECM prepared by extensively proliferated MSCs was less effective to recover their proliferative capacity than that prepared by less proliferated cells. Our results suggest that a cell culture substrate to expand MSCs requires abundance in collagen and basement membrane components, and steric, porous and fibrous structure in which ECM molecules are tightly associated.  相似文献   

Regolith migration and sorting on asteroid Itokawa     

Miyamoto H  Yano H  Scheeres DJ  Abe S  Barnouin-Jha O  Cheng AF  Demura H  Gaskell RW  Hirata N  Ishiguro M  Michikami T  Nakamura AM  Nakamura R  Saito J  Sasaki S 《Science (New York, N.Y.)》2007,316(5827):1011-1014

High-resolution images of the surface of asteroid Itokawa from the Hayabusa mission reveal it to be covered with unconsolidated millimeter-sized and larger gravels. Locations and morphologic characteristics of this gravel indicate that Itokawa has experienced considerable vibrations, which have triggered global-scale granular processes in its dry, vacuum, microgravity environment. These processes likely include granular convection, landslide-like granular migrations, and particle sorting, resulting in the segregation of the fine gravels into areas of potential lows. Granular processes become major resurfacing processes because of Itokawa's small size, implying that they can occur on other small asteroids should those have regolith.  相似文献   

Improvement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handling     

Ryosuke KANEKO  Toshie KAKINUMA  Sachiko SATO  Atsushi JINNO-OUE 《The Journal of reproduction and development》2021,67(3):235

Saving space for sperm cryopreservation would aid mouse genetics research. We previously developed the ST (sperm freezing in ShorT STraw to reduce STorage space) method for cryopreserving mouse sperm in a smaller storage space than conventional methods. However, our ST method has two drawbacks: difficulties during freeze-thaw procedures and the potential risk of sperm loss during storage. Here, we refine ST, terming the new method improved ST (iST). In iST, the straw has an air-permeable filter and the straw container (2-ml cryotube) is endowed with air vents. As in our ST method, iST frozen-thawed sperm showed good performance upon in vitro fertilization. Moreover, up to nine straws can be stored in one cryotube, occupying less storage space than conventional methods. This method provides an easy and space-saving cryopreservation method for mouse sperm, and thus will be valuable for mouse genetics researchers.  相似文献