Transgenic modification of the n‐3 HUFA biosynthetic pathway in nibe croaker larvae: improved DPA (docosapentaenoic acid; 22:5n‐3) production          下载免费PDF全文

N. Kabeya  Y. Takeuchi  R. Yazawa  Y. Haga  S. Satoh  G. Yoshizaki 《Aquaculture Nutrition》2016,22(2):472-478

Marine fish are generally unable to produce sufficient quantities of n‐3 highly unsaturated fatty acid (n‐3 HUFA) such as eicosapentaenoic acid (EPA; 20:5n‐3) and docosahexaenoic acid (DHA; 22:6n‐3). Consequently, the seed production of marine fish requires careful nutritional enrichment of live feeds such as rotifers and brine shrimp Artemia to meet n‐3 HUFA requirements for normal growth. Another strategy for improving n‐3 HUFA availability is modifying the biosynthetic pathway of marine fish using transgenic technology. In this study, we conducted a feeding trial with non‐transgenic and transgenic nibe croaker Nibea mitsukurii carrying the elongation of very long‐chain fatty acids protein 2 (Elovl2) gene isolated from masu salmon Oncorhynchus masou and three groups of Artemia (non‐enriched and enriched with two products). For all Artemia groups, docosapentaenoic acid (DPA, 22:5n‐3), which is a direct product of Elovl2, was significantly higher in the transgenic fish than that in non‐transgenic fish, despite the absence of DPA in all diets. Thus, applying transgenic techniques to marine fish at the larval stage are a powerful strategy for modifying n‐3 HUFA biosynthetic pathways.  相似文献   

Chilli pepper rugose mosaic disease caused by Pepper veinal mottle virus occurs on Ishigaki Island,Japan     

Kosuke Matsumoto  Ryosuke Yasaka  Tamotsu Setoyama  Shinji Kawano  Kazusato Ohshima 《Journal of General Plant Pathology》2016,82(1):57-60

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四类全球土地覆盖数据在中国区域的精度评价   总被引：7，自引：0，他引：7  

吴文斌  杨鹏  张莉  唐华俊  周清波  Shibasaki Ryosuke 《农业工程学报》2009,25(12):167-173

该研究以中国耕地类别为研究对象,选择2000年中国土地利用数据（NLCD-2000）为参考数据,利用比较分析法,从面积数量精度和空间位置精度两方面对目前4类全球土地覆盖数据（UMD、IGBP-DISCover、MODIS和GLC2000）产品进行了精度验证,并分析研究了4类数据精度的异同性。结果表明,4类全球数据对中国耕地数量特征和空间位置特征的估测具有明显的区域差异性。MODIS数据集和GLC2000数据集对中国耕地制图的总体精度要高于UMD数据集和IGBP-DISCover数据集。4类数据制图精度高的区域主要分布在中国的农业主产区,而误差大的区域主要分布在中国山区或耕地比例低的区域。低空间分辨率的信息源、基于像元的分类方法,以及中国复杂地形特征是4类全球土地覆盖数据精度差异的主要原因。  相似文献   

基于NDVI数据的华北地区耕地物候空间格局   总被引：13，自引：4，他引：13  

吴文斌  杨鹏  唐华俊  周清波  张莉 《中国农业科学》2009,42(2):552-560

 【目的】探讨基于多时相遥感信息监测中国华北地区耕地种植制度和物候空间格局特征。【方法】选择NDVI时间序列数据,采用非对称性高斯函数拟合方法重建平滑曲线,依据年内NDVI变化曲线峰值数目监测华北地区耕地的多熟种植制度,并利用动态阈值法获取该种植制度下耕地物候空间格局。【结果】华北地区耕地种植制度以一年两熟为主,其分布具有明显的空间差异性,随着纬度递减呈现出从简单到复杂的总体趋势。在该种植制度下,华北地区耕地第一季作物的生长开始期和生长结束期存在十分明显的空间差异,一年两熟区域的第一季作物生长开始期和生长结束期要明显早于一熟区域。与第一季作物物候期明显的空间差异相比,华北地区耕地第二季作物物候期差异不显著。【结论】华北地区耕地种植制度与物候分布格局和自然地理环境紧密相关,不同区域的温度、降水和光照等气候资源的禀赋和匹配程度对该区域的种植结构和作物布局有很大影响。此外,这种耕地物候空间格局还与作物品质、耕作水平、灌溉、施肥和农药等有密切关系。如何区别生态环境因子和人类活动因子对耕地物候的影响是今后值得深入研究的问题。  相似文献   

Spawning induction and seed production of Eastern little tuna,Euthynnus affinis (Cantor, 1849), in the post‐ and pre‐spawning seasons by hormonal treatment in a semi‐closed recirculation system with elevated temperature          下载免费PDF全文

Ryosuke Yazawa  Yutaka Takeuchi  Kenta Satoh  Yuri Machida  Kotaro Amezawa  Naoki Kabeya  Goro Yoshizaki 《Aquaculture Research》2017,48(7):3472-3481

We previously established a method for spawning induction in Eastern little tuna (ELT) Euthynnus affinis (Cantor, 1849) by administering a gonadotropin‐releasing hormone analog (GnRHa) during the natural spawning season in Japan (August–October). In order to establish seed production of ELT in the off‐spawning season, we first conducted three spawning induction trials by GnRHa administration from October 2011 to January 2012 using ELT broodstock (2 years old; three females and four males) maintained in a 10‐m³ tank with a semi‐closed recirculation system and static elevated temperature. Average water temperature and daily egg production in three trials lasting 11–15 days were 27.0 ± 0.09°C and 268 173 eggs (Trial 1), 27.0 ± 0.11°C and 277 9098 eggs (Trial 2), and 25.5 ± 0.39°C and 291 113 eggs (Trial 3) respectively. Mean fertilization rate and mean hatching rate were 70.4% and 60.5% (Trial 1), 83.9% and 79.6% (Trial 2), and 62.5% and 57.4% (Trial 3) respectively. We also succeeded in producing ELT larvae in the pre‐spawning season (April–July), although the quantity and quality of larvae produced were inferior to those produced in other calendar months. In trials involving periodic GnRHa administration during the off‐spawning seasons, hatched larvae were obtained in the 10‐m³ tank after six of nine administrations in the 2011–2012 off‐spawning season and in 16 of 19 administrations in the 2012–2013 off‐spawning season. The findings of this study demonstrated that hormonal treatment and thermal control could be used to extend the spawning period in ELT, potentially allowing larval production in the post‐ and pre‐spawning seasons.  相似文献   

Molecular analysis of multidrug resistance in feline lymphoma cells     

Okai Y  Nakamura N  Matsushiro H  Kato H  Setoguchi A  Yazawa M  Okuda M  Watari T  Hasegawa A  Tsujimoto H 《American journal of veterinary research》2000,61(9):1122-1127

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Serological diagnosis of enzootic pneumonia of swine by a double-sandwich enzyme-linked immunosorbent assay using a monoclonal antibody and recombinant antigen (P46) of Mycoplasma hyopneumoniae     

Okada M  Asai T  Futo S  Mori Y  Mukai T  Yazawa S  Uto T  Shibata I  Sato S 《Veterinary microbiology》2005,105(3-4):251-259

To facilitate the control of enzootic pneumonia (EP) of swine caused by Mycoplasma hyopneumoniae, the complement fixation (CF) test has been used for the detection of M. hyopneumoniae antibodies. However, the CF test is a cumbersome and time-consuming technique and cross-reactivity are major drawbacks associated with this method. To circumvent these drawbacks, we have developed a double-sandwich enzyme-linked immunosorbent assay (ELISA), consisting of purified monoclonal antibody (Mab) against the 46 kDa surface antigen (P46) of M. hyopneumoniae and recombinant P46 protein expressed in Escherichia coli, for the detection of antibodies to M. hyopneumoniae in serum samples from pigs experimentally inoculated with M. hyopneumoniae and from naturally infected pigs, and compared the practical usefulness of ELISA using the CF test. In experimentally inoculated pigs, the CF and ELISA antibodies were detected at almost the same time, and a good correlation was demonstrated between the CF test and the ELISA. In a survey conducted on field samples, the seropositivity by ELISA in pigs of age 2-6 months was increased. At the time of slaughter, approximately 80% of the animals were seropositive for ELISA. However, a gradual decrease in the prevalence of ELISA positive samples was observed in sows with increasing parity. No correlation was seen between the results obtained with the two methods in the clinical samples. The CF test appears to have limited value for the diagnosis of EP in conventional herds because nonspecific reactions were frequently observed. Therefore, this ELISA is a useful alternative to the CF test currently used for the diagnosis of EP.  相似文献   

Detection of New Ethylene-Producing Bacteria, Pseudomonas syringae pvs. cannabina and sesami, by PCR Amplification of Genes for the Ethylene-Forming Enzyme     

Sato M  Watanabe K  Yazawa M  Takikawa Y  Nishiyama K 《Phytopathology》1997,87(12):1192-1196

ABSTRACT Strains of Pseudomonas syringae (78 strains and 43 pathovars) and other strains (79) of plant and insect origin were examined for the presence of the ethylene-forming enzyme gene (efe) by polymerase chain reaction (PCR) assay. The sequence of the efe gene of P. syringae pv. phaseolicola PK2 was used to design two primer sets for amplification of the gene. In addition to P.syringae pv. phaseolicola (the "kudzu strain") and P.syringae pv. glycinea, which were efficient ethylene producers, several strains of P.syringae pvs. sesami and cannabina generated PCR products of the predicted size. A DNA probe of the efe gene, isolated from strain PK2, hybridized to these PCR products, indicating homology to the P.syringae pv. phaseolicola efe gene. PCR restriction fragment length polymorphism analyses suggested that these four pathovars harbor a similar efe gene. Furthermore, the probe hybridized to an indigenous plasmid of P.syringae pv. cannabina, suggesting that the efe gene could be located on a plasmid in this pathovar, but did not hybridize to plas-mids of P.syringae pv. sesami strains. P.syringae pvs. sesami and cannabina strains produced ethylene in King's medium B at levels similar to those of P.syringae pvs. phaseolicola and glycinea. Thus, two new ethylene-producing bacteria were detected by the PCR assay.  相似文献   

Implantation of Tetrapod-Shaped Granular Artificial Bones or β-Tricalcium Phosphate Granules in a Canine Large Bone-Defect Model     

Sungjin CHOI  I-Li LIU  Kenichi YAMAMOTO  Muneki HONNAMI  Takamasa SAKAI  Shinsuke OHBA  Ryosuke ECHIGO  Shigeki SUZUKI  Ryouhei NISHIMURA  Ung-il CHUNG  Nobuo SASAKI  Manabu MOCHIZUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(2):229-235

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Suppressive effect of hyaluronan on chondrocyte apoptosis in experimentally induced acute osteoarthritis in dogs     

Echigo R  Mochizuki M  Nishimura R  Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(8):899-902

Dogs receiving anterior cruciate ligament transection (ACLT) were treated with either intravenous (IV) or intraarticular (IA) administration of hyaluronan (HA), and differences in appearance of chondrocyte apoptosis of the stifle joint were investigated. Chondrocyte apoptosis was detected using flow cytometry as well as by staining with TdT-mediated dUTP nick end labeling (TUNEL). The percentage of apoptotic chondrocytes in dogs with ACLT was significantly higher than that in intact (non-ACLT) dogs. Dogs treated with IA or IV injection of HA after ACLT had fewer apoptotic chondrocytes than non-treated dogs after ACLT. It was suggested that ACLT-induced apoptosis of chondrocytes was suppressed by HA administration of either IA or IV.  相似文献