Quantitative PCR detection of feline morbillivirus in cat urine samples     

Tetsuya FURUYA  Akiko WACHI  Yukiko SASSA  Tsutomu OMATSU  Makoto NAGAI  Ryuji FUKUSHIMA  Makoto SHIBUTANI  Tomohiro YAMAGUCHI  Yosuke UEMATSU  Kinji SHIROTA  Tetsuya MIZUTANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1701-1703

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Effect of monensin withdrawal on rumen fermentation,methanogenesis and microbial populations in cattle          下载免费PDF全文

Arfan Abrar  Takamitsu Tsukahara  Makoto Kondo  Tomomi Ban‐Tokuda  Wang Chao  Hiroki Matsui 《Animal Science Journal》2015,86(9):849-854

This study was designed to obtain information on the residual influence of dietary monensin on ruminant fermentation, methanogenesis and bacterial population. Three ruminally cannulated crossbreed heifers (14 months old, 363 ± 11 kg) were fed Italian ryegrass straw and concentrate supplemented with monensin for 21 days before sampling. Rumen fluid samples were collected for analysis of short chain fatty acid (SCFA) profiles, monensin concentration, methanogens and rumen bacterial density. Post‐feeding rumen fluid was also collected to determine in vitro gas production. Monensin was eliminated from the rumen fluid within 3 days. The composition of SCFA varied after elimination of monensin, while total production of SCFA was 1.78 times higher than on the first day. Methane production increased 7 days after monensin administration ceased, whereas hydrogen production decreased. The methanogens and rumen bacterial copy numbers were unaffected by the withdrawal of monensin.  相似文献   

Changes in estrogen receptor expression in the chick thymus during late embryonic development     

Masafumi Katayama  Tomokazu Fukuda  Toshimitsu Hatabu  Kiyoaki Narabara  Asaki Abe  Yasuhiro Kondo 《Animal Science Journal》2014,85(3):277-285

In chickens, although estrogen receptors (ER) are reported to be associated with the immunological processes, detailed information about the differences in ER expression in the tissues related to the development of lymphocytes is not fully known, especially during the developmental stage. To learn more about this immunological relationship, we used semi‐quantitative polymerase chain reaction method to detect the ER expression levels in the thymus tissues of chicks during the developmental stage. Furthermore, ER‐expressing cells were detected by immunohistochemistry. The results of this study show that the expression level of ER increased on embryonic day 16 and decreased on day 20. Furthermore, ER expression was significantly higher in male than in female chickens at day 16. The increased expression on day 16 and decreased level on day 20 were also reproduced in the incidence of immunoreactive cells, although there was a 1‐day delay in the elevated incidence of the cells. This study revealed the changes in ER expression and the incidence of ER‐positive cells in the thymus of chickens during the developmental stage.  相似文献   

Polymerase chain reaction (PCR) amplification of bacilliform virus (RV-PJ) DNA in Penaeus japonicus Bate and systemic ectodermal and mesodermal baculovirus (SEMBV) DNA in Penaeus monodon Fabricius   总被引：3，自引：0，他引：3  

Y Takahashi  T Itami  M Maeda  N Suzuki  J Kasornchandra  K Supamattaya  R Khongpradit  S Boonyaratpalin  M Kondo  K Kawai  R Kusuda  I Hirono  T Aoki 《Journal of fish diseases》1996,19(5):399-403

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Characterization of an Alginate Lyase,FlAlyA, from Flavobacterium sp. Strain UMI-01 and Its Expression in Escherichia coli     

Akira Inoue  Kohei Takadono  Ryuji Nishiyama  Kenji Tajima  Takanori Kobayashi  Takao Ojima 《Marine drugs》2014,12(8):4693-4712

A major alginate lyase, FlAlyA, was purified from the periplasmic fraction of an alginate-assimilating bacterium, Flavobacterium sp. strain UMI-01. FlAlyA showed a single band of ~30 kDa on SDS-PAGE and exhibited the optimal temperature and pH at 55 °C and pH 7.7, respectively. Analyses for substrate preference and reaction products indicated that FlAlyA was an endolytic poly(mannuronate) lyase (EC 4.2.2.3). A gene fragment encoding the amino-acid sequence of 288 residues for FlAlyA was amplified by inverse PCR. The N-terminal region of 21 residues except for the initiation Met in the deduced sequence was predicted as the signal peptide and the following region of six residues was regarded as propeptide, while the C-terminal region of 260 residues was regarded as the polysaccharide-lyase-family-7-type catalytic domain. The entire coding region for FlAlyA was subjected to the pCold I—Escherichia coli BL21(DE3) expression system and ~eight times higher yield of recombinant FlAlyA (recFlAlyA) than that of native FlAlyA was achieved. The recFlAlyA recovered in the periplasmic fraction of E. coli had lost the signal peptide region along with the N-terminal 3 residues of propeptide region. This suggested that the signal peptide of FlAlyA could function in part in E. coli.  相似文献   

Botulism among penned pheasants and protection by vaccination with C1 toxoid     

H Kurazono  K Shimozawa  G Sakaguchi  M Takahashi  T Shimizu  H Kondo 《Research in veterinary science》1985,38(1):104-108

Fifty to 100 per cent of about 8000 penned pheasants raised on a farm in Hiroshima Prefecture died annually for three years. Deaths were ascribed to type C botulism. Vaccination of four groups of pheasants with partially purified C1 toxoid effectively protected them against type C botulism. The protective efficacy of the toxoid was emphasised by the relatively high susceptibility of the pheasant to C1 toxin.  相似文献   

Evaluation of biological activities of extracts from the fruiting body of <Emphasis Type="Italic">Pleurotus citrinopileatus</Emphasis> for skin cosmetics     

Tian-Xiao Meng  Shoko Furuta  Sayaka Fukamizu  Ryoko Yamamoto  Hiroya Ishikawa  Enos Tangke Arung  Kuniyoshi Shimizu  Shoji Ohga  Ryuichiro Kondo 《Journal of Wood Science》2011,57(5):452-458

Pleurotus citrinopileatus Singer has recently become a popular delicacy in East Asian countries. We prepared a methanol extract, soluble fractions from the methanol extract, and a hot water extract of the fruiting bodies of P. citrinopileatus. The biological activities such as melanin biosynthesis inhibition, tyrosinase inhibition, antioxidant activities [1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, oxygen radical absorbance capacity (ORAC), and superoxide dismutase (SOD)-like activity], antibacterial activities, and antihyaluronidase activities of these extracts were evaluated. We found that the n-hexanesoluble, diethyl ether-soluble, and ethyl acetate-soluble fractions exhibited melanin biosynthesis inhibition in B16 melanoma cells, as well as antioxidant (ORAC) and antibacterial activities. However, the n-butanol-soluble and water-soluble fractions and the methanol and hot water extracts exhibited antioxidant (DPPH radical scavenging, SOD-like activity) and antihyaluronidase activities. These results indicate that the fruiting bodies of P. citrinopileatus have the potential to be used as an ingredient in skin cosmetics.  相似文献   

旱稻水分利用率与碳同位素识别值之间的关系     

赵炳梓  张佳宝  近藤始彦  前田守弘  尾崎保夫 《土壤学报》2004,41(5):707-714

通过温室盆栽试验 ,分析和探讨了三个水平的土壤水分条件对分蘖期和成熟期收获的旱稻(OryzasativaL .)生物量累积、水分利用率 (WUE)、植株不同部位的碳同位素识别值 (CID)的影响 ,并了解了它们之间的相互关系。水分条件包括 :饱和含水量 (W1 )、饱和含水量的 70 %(W2 )、饱和含水量的 4 0 %(W3)。结果表明当土壤水分条件从W1降低到W2时 ,分蘖期收获的生物量降低 4 5 %左右 ,成熟期收获的生物量降低 1 6 %～ 1 9%;而当从W1降低到W3时 ,分蘖期收获的生物量降低 73%左右 ,成熟期收获的生物量降低 5 5 %～ 5 7%。然而 ,根据地上部干重计算而来的WUE(WUE 地上部 )和根据全株干重计算而来的WUE(WUE 全株 )则随土壤含水量的降低而增加 ,其增幅在分蘖期为 0 .0 7～ 0 .2 8gkg-1 ,在成熟期为 0 .0 7～ 0 .4 5gkg-1 。植株的CID值变幅为 1 7.0～ 2 0 .6 ,但植株不同部位间差别显著 ,分蘖期收获的样品CID值从小到大的顺序为 :根 <最近完全伸展叶 <叶芽 <茎秆 ;而成熟期收获的样品CID值从小到大的顺序为 :籽粒 <根 <茎秆 <旗叶。随着土壤含水量的降低 ,植株所有部位的CID值亦显著减小。叶部的CID值与WUE 地上部(和WUE 全株 )之间呈一致的负相关关系。  相似文献   

Macronutrient accumulation in wheat crop (Triticum aestivum L.) with Azospirillum brasilense associated with nitrogen doses and sources     

Fernando Shintate Galindo  Marcelo Carvalho Minhoto Teixeira Filho  Salatiér Buzetti  José Mateus Kondo Santini  Eduardo Henrique Marcandalli Boleta  Willian Lima Rodrigues 《Journal of plant nutrition》2020,43(8):1057-1069

Abstract

New studies are needed to optimize the nitrogen (N) amount that can be applied to utilize the Azospirillum brasilense benefits. In addition, information regarding the interaction between the urease inhibitor and biological nitrogen fixation (BNF) and how they affect the macronutrients accumulation are also needed. We evaluate the effect of N sources and doses associated with A. brasilense regarding the macronutrients accumulation in straw and grains and wheat grain yield in tropical conditions. A randomized block experimental design was used with four replications in a 2?×?5?×?2 factorial arrangement as follows: two N sources (urea and urea with urease enzyme inhibitor NBPT; five N doses (0, 50, 100, 150, and 200?kg ha^?1) applied in topdressing; with and without A. brasilense inoculation. We found that an increase in N doses positively influenced the accumulation of macronutrients in straw and grains and the wheat grain yield. N sources have similar effects. Inoculation with A. brasilense increased accumulation of Mg and S in straw and P, Ca, and Mg in grains, regardless of the N dose. The inoculation with A. brasilense associated with 140?kg ha^?1 of N increased wheat grain yield. The inoculation can contribute in a more sustainable way to wheat nutrition and optimizing N fertilization.  相似文献   

Effects of tyrosine administration on serum biopterin in normal controls and patients with Parkinson's disease     

T Yamaguchi  T Nagatsu  T Sugimoto  S Matsuura  T Kondo  R Iizuka  H Narabayashi 《Science (New York, N.Y.)》1983,219(4580):75-77

After administration of tyrosine, total concentration of biopterin, the cofactor for tyrosine hydroxylase, was increased in the striatum, adrenal glands, and serum of rats, and in the serum of humans. Serum biopterin is lower in patients with Parkinson's disease than in normal controls. After oral administration of tyrosine, the increase in serum biopterin concentration was smaller in patients with Parkinson's disease (less than twofold) than in healthy controls (three-to sevenfold). These results suggest that tyrosine may have a regulatory role in biopterin biosynthesis and that patients with Parkinson's disease may have some abnormality in the regulation of biopterin biosynthesis.  相似文献