Capacitation of Frozen Thawed Buffalo Bull (Bubalus bubalis) Spermatozoa with Higher Heparin Concentrations     

A Mehmood  M Anwar  SM Saqlan Naqvi 《Reproduction in domestic animals》2007,42(4):376-379

The present study was conducted to examine the effect of high heparin concentration on capacitation of buffalo spermatozoa with a short incubation time. Frozen thawed spermatozoa from three buffalo bulls were pooled and treated with either 50, 100 or 200 microg/ml heparin for 30 min. Capacitation was evaluated by acrosome reaction of spermatozoa and in vitro fertilization rate (per cent cleavage rate, per cent cleavage index). Acrosome reaction was induced in heparin treated spermatozoa with calcium ionophore A23187 and staining was carried out with Coomassie G-250 to evaluate the response as compared with control (0 heparin + calcium ionophore). Significantly higher percentage of acrosome reaction (AR) spermatozoa was noted after heparin treatment (36.8-48.2%) as compared with control (8.1% ; p < 0.05) but differences among the three heparin concentrations were non-significant. However, a significantly higher in vitro fertilization rate was recorded in spermatozoa capacitated by 50 and 100 microg/ml heparin (80.4 and 75.9% cleavage rate, respectively) as compared with 200 microg/ml heparin (47.2% cleavage rate; p < 0.001). It is concluded that buffalo spermatozoa capacitated with 50-100 microg/ml heparin had significantly higher ability to improve in vitro fertilization rate in buffalo.  相似文献   

Hydatid disease in goats in Bangladesh     

A.W.M. Shamsul Islam 《Veterinary parasitology》1980,7(2):103-107

A total of 12 344 goats of different ages from various localities of Bangladesh were examined for the presence of hydatid disease and 1024 (8.29%) were found to be infected. The incidence of infection by organ was: liver 36.72%, lungs 32.03%, spleen 4.69%, heart 3.13%, kidney 1.56%, omentum 0.78%, and both liver and lungs 21.09%. The severity of infection was: weak (84.56% and 86.55%), medium (13.32% and 11.87%) and heavy (2.12 and 1.58%) in the liver and lungs respectively. Of the 1792 cysts examined, 175 (9.76%) were fertile, 1342 (74.89%) were sterile, 206 (11.50%) were calcified, 47 (2.62%) were supporative and 22 (1.23%) were undeveloped. The incidence of infection by age of host was: 0.63% between 0 and 1 year, 2.48% between 1 and 2 years, 5.53% between 2 and 3 years, 16.80% between 3 and 5 years and 21.64% for 5 years old and above.  相似文献   

Molecular and antigenic characterization of Bangladeshi isolates of infectious bursal disease virus demonstrate their similarities with recent European, Asian and African very virulent strains     

Islam MR  Zierenberg K  Eterradossi N  Toquin D  Rivallan G  Müller H 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2001,48(3):211-221

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Temperature and metal ions-dependent activity of the family I inorganic pyrophosphatase from the swine roundworm Ascaris suum     

Islam KM  Miyoshi T  Isobe T  Kasuga-Aoki H  Arakawa T  Matsumoto Y  Yokomizo Y  Tsuji N  Tsuji N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(2):221-223

Temperature dependence, heat stability and metal ions-dependent activity were examined on the Family I inorganic pyrophosphatase (PPase) recently identified from Ascaris suum. Recombinant A. suum PPase (rAsPPase) showed an optimal activity at 55 degrees C. The rAsPPase was heat stable at 40 degrees C in the absence of added Mg(2+) and at 50 degrees C in its presence. The enzyme required divalent metal ions for its activity. The preferences for the metal ions (5 mM concentration) were in the order: Mg(2+)> Co(2+)> Cu(2+)> Fe(2+)> Zn(2+)> Mn(2+). On the contrary, enzyme activity was inhibited by Ca(2+). These findings suggest that catalytic features of AsPPase are consistent with the Family I PPases reported from a wide range of organisms.  相似文献   

Constituents and cytotoxicity of Zanthoxylum rhesta stem bark     

Monira Ahsan  Tahmina Akter Zaman  Choudhury M. Hasan  Chihiro Ito  S. K. Nazrul Islam   《Fitoterapia》2000,71(6)

3,5-Dimethoxy-4-geranyloxycinnamyl alcohol (1), 8-methoxy-N-methylflindersine (2), xanthyletin and sesamin have been isolated from petroleum ether extract of the stem bark of Zanthoxylum rhesta. The petroleum ether extract and 8-methoxy-N-methylflindersine showed cytotoxicity on brine shrimp nauplii.  相似文献   

Analysis of the mating system,reproductive characteristics,and spatial genetic structure in a natural mangrove tree (Bruguiera gymnorrhiza) population at its northern biogeographic limit in the southern Japanese archipelago     

Md Sajedul Islam  Chunlan Lian  Norikazu Kameyama  Taizo Hogetsu 《Journal of Forest Research》2015,20(2):293-300

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Effect of Tree Diversity on Soil Organic Carbon Content in the Homegarden Agroforestry System of North-Eastern Bangladesh     

Islam  Mahmuda  Dey  Anna  Rahman  Mizanur 《Small-Scale Forestry》2015,14(1):91-101

Small-scale Forestry - Tropical agroforestry systems have immense potential to sequester carbon both in aboveground and belowground biomass and soil. Homegarden agroforestry is a popular land-use...  相似文献   

Forest-based betel leaf and betel nut People in Bangladesh： approach Lawachara National Park （LNP） farming of the Khasia indigenous to biodiversity conservation in     

Md. Jahirul Islam  Tapan Kumar Nath 《林业研究》2014,25(2):419-427

An exploratory survey was conducted among the Khasia living in Lawachara National Parkt （LNP） to investigate their depend- ency on the protected area （PA） for livelihoods, betel leaf hill farming, traditional forest conservation and perceptions of the PA, Field data were collected by interviewing 48 household heads from two villages （punjis） located inside the LNP and visiting their farms.： The economy of the Khasia was forest-based. They were largely dependent on betel leaf hill farming in LNP and nearly 71% of their mean annual income was de- rived from this irrespective of farmer category. On average, about 14% of the incomes of the poorer farmers came from forest produce followed by 10% and 6% for medium and rich farmers respectively. Hills and forests were the foundations of their lives and livelihoods, and LNP was the life-blood of Khasia survival. As a sustainable production system, this farming practice plays a vital role in conserving biodiversity in LNP and might be replicated elsewhere.  相似文献   

Impacts of Nighttime Warming on the Soil Nematode Community in a Winter Wheat Field of Yangtze Delta Plain,China     

SONG Zhen-wei  ;ZHANG Bin  ;TIAN Yun-lu  ;DENG Ai-xing  ;ZHENG Cheng-yan  ;Md Nurul Islam  ;Md Abdul Mannaf  ;ZHANG Wei-jian 《中国农业科学(英文版)》2014,(7):1477-1485

Changes in the soil nematode community induced by global warming may have a considerable influence on agro-ecosystem functioning. However, the impacts of predicted warming on nematode community in farmland （e.g., winter wheat field） have not been well documented. Therefore, a field experiment with free air temperature increase （FATI） was conducted to investigate the responses of the soil nematode community to nighttime warming in a winter wheat field of Yangtze Delta Plain, China, during 2007 to 2009. Nighttime warming （NW） by 1.8~C at 5-cm soil depth had no significant impact on the total nematode abundance compared to un-warmed control （CK）. However, NW significantly affected the nematode community structure. Warming favored the bacterivores and fungivores, such as Acrobeles, Monhystera, Rhabditis, and Rhabdontolaimus in bacterivores, and Filenchus in fungivores, while the plant-parasites were hindered, such as Helicotylenchus and Psilenchus. Interestingly, the carnivores/ omnivores remained almost unchanged. Hence, the abundances ofbacterivores and fungivores were significantly higher under NW than those under CK. Similarly, the abundances of plant-parasites were significantly lower under NW than under CK. Furthermore, Wasilewska index of the nematode community was significantly higher under NW than those under CK, indicating beneficial effect to the plant in the soil. Our results suggest that nighttime warming may improve soil fertility and decrease soil- borne diseases in winter wheat field through affecting the soil nematode community. It is also indicated that nighttime warming may promote the sustainability of the nematode community by altering genera-specific habitat suitability for soil biota.  相似文献   

Qualitative risk assessment of transmission pathways of highly pathogenic avian influenza (HPAI) virus at live poultry markets in Dhaka city,Bangladesh     

SK Shaheenur Islam  Holy Akwar  Md. Mehedi Hossain  Md. Abu Sufian  Md. Zakiul Hasan  Shovon Chakma  Tongkorn Meeyam  Warangkhana Chaisowwong  Veerasak Punyapornwithaya  Nitish C. Debnath  Eric Brum  Duangporn Pichpol 《Zoonoses and public health》2020,67(6):658-672

Analysis of environmental samples obtained from the Live Poultry Markets (LPMs) of Dhaka City, Bangladesh, has revealed that the highest degree of prevalence of highly pathogenic avian influenza A (HPAI, H5N1), besides other subtypes of the LPAI virus, poses the plausible risk of transmission of these viruses between human and poultry species. The present study was conducted using the OIE risk analysis framework to assess the risk level of each pathway successively. The estimated risk parameters were integrated towards to obtain the overall risk level for each specific HPAI transmission pathway using the matrix adapted by Cristobel Zepeda accompanying other expert consultations. The relevant data obtained from published and unpublished sources, together with survey data of field observations, were used to formulate and confirm the risk pathways and their associated risks. The results revealed that the risk of the release of the HPAI virus was medium when exposure was high. Additionally, the consequence would be considered very high with a medium degree of uncertainty for all parameters. Ultimately, the overall risk for transmission was estimated as medium with a medium degree of uncertainty. The findings of this study reveal that there is a significant threat that HPAI virus transmission could occur among poultry and humans and effectively sustain within the environment of the LPMs. Our findings are primarily focused on public health considerations, the hygienic slaughter of poultry and the relevant cleaning and sanitation practices conducted in the LPMs to support evidence‐based decision‐making processes. The findings of the study have the potential to be used to formulate effective risk reduction measures and can be further adapted in low‐resource settings without major infrastructural changes required of the LPMs. All of which would reduce the risk of HPAI virus release and further lessen the degree of exposure and transmission in established LPMs.  相似文献