Ovaprim treatment promotes oocyte development and milt fertilization rate in diploid and triploid African catfish (<Emphasis Type="Italic">Clarias gariepinus)</Emphasis>     

Ali Karami  Annie Christianus  Hadi Zokaeifar  Khairul Zamri Saad  Fahmi T. J. Imraan  Shahram Shakibazadeh  Hossien Negarestan  Simon C. Courtenay 《Aquaculture International》2011,19(6):1025-1034

Triploid fish are increasingly used in aquaculture because they are generally unable to reproduce successfully. Energy is channeled into somatic growth rather than gonadal development, and in the event of escape, the animals are unlikely to breed successfully among themselves or with wild conspecifics. This study tested the ability of recently matured triploid African catfish (Clarias gariepinus) to produce and fertilize eggs with and without ovaprim treatment. Triploid females did not show the increase in ovary size observed in diploid members of the same cohort between 8 and 9 months of age, or the coincident decrease in visceral fat deposits, and this was unaffected by up to 5 weekly i.m. injections of 0.5 ml kg⁻¹ Ovaprim. However, we observed advanced vitellogenin (Vtg) sequestration in oocytes of triploid females, albeit to a lesser degree and with lesser cortical alveoli, compared to oocytes from diploid cohort members. Histological sections revealed a positive trend of oocyte development up to the third weekly ovaprim injection followed by a negative gonadal development in weeks four and five. Milt from triploid males injected 9–12 h earlier with 0.25 ml kg⁻¹ ovaprim i.m. fertilized more diploid eggs than milt from untreated triploid males (30 vs. 20%), but none of the developing embryos of triploid paternity survived to hatch. In contrast, milt of diploid males fertilized 49% of eggs, and 20% of the developing embryos hatched successfully. These rates were improved in ovaprim-injected diploid males to 70% fertilization and 33% hatch. This study demonstrates potential of overcoming non-viability of eggs from triploid female African catfish, and enhancing the ability of triploid milt to fertilize eggs.  相似文献   

Pathogenicity of <Emphasis Type="Italic">Beauveria bassiana</Emphasis> and <Emphasis Type="Italic">Metarhizium anisopliae</Emphasis> against <Emphasis Type="Italic">Galleria mellonella</Emphasis>     

Khalid A. Hussein  Mohamed A. A. Abdel-Rahman  Ahmed Y. Abdel-Mallek  Saad S. El-Maraghy  Jin Ho Joo 《Phytoparasitica》2012,40(2):117-126

The greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae) is occasionally found in beehives and is a major pest of stored wax. Entomopathogenic fungi have recently received attention as possible biocontrol elements for certain insect pests. In this study, 90 isolates of Beauveria bassiana and 15 isolates of Metarhizium anisopliae were screened for proteases and lipases production. The results showed significant variations in the enzymatic action between the isolates. In the bioassay, the selected isolates evinced high virulence against the 4th instar of the G. mellonella larvae. The isolates BbaAUMC3076, BbaAUMC3263 and ManAUMC3085 realized 100% mortality at concentrations of 5.5 × 10⁶ conidia ml⁻¹, 5.86 × 10⁵ conidia ml⁻¹, and 4.8 × 10⁶ conidia ml⁻¹, respectively. Strong enzymatic activities in vitro did not necessarily indicate high virulence against the tested insect pest. The cuticle of the infected larvae became dark and black-spotted, indicating direct attack of fungus on the defense system of the insects. The LC₅₀ values were 1.43 × 10³, 1.04 × 10⁵ and 5.06 × 10⁴ for Bba3263AUMC, Bba3076AUMC and Man3085AUMC, respectively, and their slopes were determined by computerized probit analysis program as 0.738 ± 0.008, 0.635 ± 0.007 and 1.120 ± 0.024, respectively.  相似文献   

Transmission of Streptococcus agalactiae from a hatchery into a newly established red hybrid tilapia,Oreochromis niloticus (L.) × Oreochromis mossambicus (Peters), farm     

M N A Amal  M Zamri‐Saad  A Siti‐Zahrah  A R Zulkafli 《Journal of fish diseases》2013,36(8):735-739

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Occurrence and distribution of chemical pollutants in Lake Mariut,Egypt. II. Heavy metals     

Massoud A. H. Saad  A. A. Ezzat  O. A. El-Rayis  H. Hafez 《Water, air, and soil pollution》1981,16(4):401-407

The occurrence and distribution of heavy metals in the water of Lake Mariut, a heavily polluted brackish water lake in Egypt, and their accumulation in the different parts ofTilapia fish in this lake were studied. The variations in concentrations of the metals (Zn, Cu, Fe, Mn, and Cd) in the lake water are mostly due to variations in the discharge rate of dumped wastes. The mean concentrations of these metals in different fish parts were much higher than those in the lake water.  相似文献   

Metals and chlorinated hydrocarbons in surficial sediments of three nile Delta lakes,Egypt     

Saad  Massoud A. H.  McComas  Steven R.  Eisenreich  Steven J. 《Water, air, and soil pollution》1985,24(1):27-39

Water, Air, & Soil Pollution - Surficial sediment samples from three shallow Nile Delta lakes (Lake Mariut, Hozha hydrodrome, andke Manzalah) were analyzed for PCBs, DDT-group pesticides, and...  相似文献   

Both experimental hypo- and hyper-thyroidism exacerbate the adverse effects of chronic heat stress in broilers     

R. A. Al wakeel  M. F. Saad  A. Abdel Azeez  F. Elkhiat  M. Shukry 《British poultry science》2019,60(3):330-339

1. The effects of hypo- and hyper-thyroidism in mitigating or exacerbating the negative changes of chronic heat stress (HS) in broilers were investigated.

2. Three-week-old broilers were distributed into six groups (n = 13 per group). Three groups were housed at ambient room temperature: control group (CN), propylthiouracil-treated group (AN) and thyroxine-treated group (TN). The other three groups were exposed to HS at 33 ± 1°C for 2 weeks: control heat stress (CH), propylthiouracil + heat stress (AH) and thyroxine + HS (TH).

3. Induced hypothyroidy significantly decreased cloacal temperature and body weight gain in the birds in both the normal and HS groups (AN, AH). Conversely, hyperthyroidy resulted in a significant elevation in cloacal temperature in the TN and TH groups and a significant decline in weight gain in the TH group. Hyperthyroidy exacerbated the HS-induced degenerative changes in jejunal mucosa and caused noticeable vascular changes. A significant increase in the expression levels of jejunal nutrient transporter genes was observed in the AH and TH groups. The hyperthyroidic state significantly upregulated the HSP70 expression level in the TH group and the reverse occurred with propylthiouracil (PTU) treatment in the AH group.

4. PTU supplementation to chicks reared under HS significantly decreased the triiodothyronine level, antibody (Ab) titre, and increased the heterophil-lymphocyte ratio. Furthermore, it induced higher hepatic glutathione peroxidase (GSH-Px) activity in the AN and AH groups and decreased the malondialdehyde content (MDA) in the AN group. Hyperthyroidy significantly increased triiodothyronine concentration, H/L ratio and decreased Hb concentration and Ab titres in the TH group. Additionally, this status increased the MDA content and decreased the GSH-Px activities.

5. In conclusion, manipulation of thyroid status is not a remedy to overcome the undesirable effects of HS in broilers.  相似文献   

Effects of a peripheral alpha2 adrenergic-receptor antagonist on the hemodynamic changes induced by medetomidine administration in conscious dogs     

Enouri SS  Kerr CL  McDonell WN  O'Sullivan ML  Neto FJ 《American journal of veterinary research》2008,69(6):728-736

OBJECTIVE: To evaluate the effects of administration of a peripheral alpha(2)-adrenergic receptor antagonist (L-659,066), with and without concurrent administration of glycopyrrolate, on cardiopulmonary effects of medetomidine administration in dogs. ANIMALS: 6 healthy adult dogs. PROCEDURES: Dogs received saline (0.9% NaCl) solution (saline group), L-659,066 (group L), or L-659,066 with glycopyrrolate (group LG). These pretreatments were followed 10 minutes later by administration of medetomidine in a randomized crossover study. Hemodynamic measurements and arterial and mixed-venous blood samples for blood gas analysis were obtained prior to pretreatment, 5 minutes after pretreatment, and after medetomidine administration at intervals up to 60 minutes. RESULTS: After pretreatment in the L and LG groups, heart rate, cardiac index, and partial pressure of oxygen in mixed-venous blood (PvO2) values were higher than those in the saline group. After medetomidine administration, heart rate, cardiac index, and PvO2 were higher and systemic vascular resistance, mean arterial blood pressure, and central venous pressure were lower in the L and LG groups than in the saline group. When the L and LG groups were compared, heart rate was greater at 5 minutes after medetomidine administration, mean arterial blood pressure was greater at 5 and 15 minutes after medetomidine administration, and central venous pressure was lower during the 60-minute period after medetomidine administration in the LG group. CONCLUSIONS AND CLINICAL RELEVANCE: Administration of L-659,066 prior to administration of medetomidine reduced medetomidine-induced cardiovascular changes in healthy dogs. No advantage was detected with concurrent administration of L-659,066 and glycopyrrolate.  相似文献   

Cardiopulmonary effects of anesthetic induction with thiopental, propofol, or a combination of ketamine hydrochloride and diazepam in dogs sedated with a combination of medetomidine and hydromorphone     

Enouri SS  Kerr CL  McDonell WN  Dyson DH 《American journal of veterinary research》2008,69(5):586-595

OBJECTIVE: To evaluate the cardiopulmonary effects of anesthetic induction with thiopental, propofol, or ketamine hydrochloride and diazepam in dogs sedated with medetomidine and hydromorphone. ANIMALS: 6 healthy adult dogs. PROCEDURES: Dogs received 3 induction regimens in a randomized crossover study. Twenty minutes after sedation with medetomidine (10 microg/kg, IV) and hydromorphone (0.05 mg/kg, IV), anesthesia was induced with ketamine-diazepam, propofol, or thiopental and then maintained with isoflurane in oxygen. Measurements were obtained prior to sedation (baseline), 10 minutes after administration of preanesthetic medications, after induction before receiving oxygen, and after the start of isoflurane-oxygen administration. RESULTS: Doses required for induction were 1.25 mg of ketamine/kg with 0.0625 mg of diazepam/kg, 1 mg of propofol/kg, and 2.5 mg of thiopental/kg. After administration of preanesthetic medications, heart rate (HR), cardiac index, and PaO(2) values were significantly lower and mean arterial blood pressure, central venous pressure, and PaCO(2) values were significantly higher than baseline values for all regimens. After induction of anesthesia, compared with postsedation values, HR was greater for ketamine-diazepam and thiopental regimens, whereas PaCO(2) tension was greater and stroke index values were lower for all regimens. After induction, PaO(2) values were significantly lower and HR and cardiac index values significantly higher for the ketamine-diazepam regimen, compared with values for the propofol and thiopental regimens. CONCLUSIONS AND CLINICAL RELEVANCE: Medetomidine and hydromorphone caused dramatic hemodynamic alterations, and at the doses used, the 3 induction regimens did not induce important additional cardiovascular alterations. However, administration of supplemental oxygen is recommended.  相似文献   

The effects of energy and protein supplementation strategy and frequency on the performance of beef cattle that grazed on Tanzania grass pastures during the rainy season     

R.L. Miorin  R.M. Saad  L.D.F. Silva  S. Galbeiro  U. Cecato  F.L. Massaro Junior 《Tropical animal health and production》2016,48(8):1561-1567

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Sediment accretion and variability of sedimentological characteristics of a tropical estuarine mangrove: Kemaman, Terengganu, Malaysia     

Shahbudin Saad  Mohd. Lokman Husain  Rosnan Yaacob  Toshiyuki Asano 《Mangroves and Salt Marshes》1999,3(1):51-58

This paper reports on a study of accretion rate and sedimentological variability in a estuarine mangrove swamp. One hundred and sixteen stations were monitored for 2 years. In addition, surface sediment samples were collected at 52 stations during the months of May, June and July (1994) to represent the nonmonsoon sediments and November, December (1994) and January (1995) to represent the monsoon sediments. Results show that the accretion rate for the first year was 1.46±0.13cm/yr and 0.66±0.04cm/yr for the second year thus making the average accretion for the 2 years period to be 1.06cm/yr. The average accretion rate for the monsoon season (0.26 ± 0.04cm/month) was found to be significantly higher than the nonmonsoon season (0.12 ± 0.03cm/month). Nevertheless, the same is not true for the surface sediment characteristics. The sedimentological characteristics between the monsoon and the nonmonsoon sediments were not significantly different.  相似文献