Production, purification, and bioassay of tentoxin   总被引：1，自引：0，他引：1  

S M Saad  J M Halloin  D J Hagedorn 《Phytopathology》1970,60(3):415-418

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Manduca sexta (Lepidoptera: Sphingidae) cadherin fragments function as synergists for Cry1A and Cry1C Bacillus thuringiensis toxins against noctuid moths Helicoverpa zea,Agrotis ipsilon and Spodoptera exigua     

Mohd Amir F Abdullah  Saad Moussa  Milton D Taylor  Michael J Adang 《Pest management science》2009,65(10):1097-1103

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Molecular characterization of a recent Newcastle disease virus outbreak in Jordan     

Ababneh MM  Dalab AE  Alsaad SR  Al-Zghoul MB  Al-Natour MQ 《Research in veterinary science》2012,93(3):1512-1514

Newcastle disease (ND) is a highly contagious viral disease and is a continuous threat to the poultry industry worldwide. In the early months of 2011, several devastating ND outbreaks occurred in Jordan affecting broilers, layers and breeders. The fusion gene of the isolated Newcastle disease virus (NDV) was partially amplified by RT-PCR, then directly sequenced. The NDV isolates were found to have the motif(112)RRQKRF(117). This motif and a mean death time (MDT) of 46h are indicative of the velogenic nature of these NDV isolates. Phylogenetic analysis showed that the new NDV strain belongs to the lineage 5d (Aldous et al., 2003) and is closely related to the Chinese strain SG/Liaoning/2009. NDV outbreaks in 2010 and 2011 have been noted in neighboring countries. Based on the high nucleotide similarity between our isolated NDV isolates and the Chinese NDV strain, the origin of these recent NDV isolates might be from China.  相似文献   

Testosterone induces lymphopenia in turtles     

A H Saad  M Torroba  A Varas  A Zapata 《Veterinary immunology and immunopathology》1991,28(2):173-180

Owing to the possible role of sex steroids in the immune-neuroendocrine interactions found in lower vertebrates, we attempted to delineate the effect of testosterone propionate on peripheral blood (PB) and the lymphoid organs of the turtle Mauremys caspica. A single intraperitoneal injection of 200 micrograms/g body weight produced thymic involution and intense lymphopenia in the spleen and, less severely, in the PB compartment. It is suggested that lymphocyte redistribution may occur among the various compartments of the body as the main effect of hormone-induced lymphocyte redistribution, although the mechanism in reptiles and mammals is not yet understood.  相似文献   

Alterations in neutrophil phagocytosis and lymphocyte blastogenesis in dairy cows around parturition   总被引：2，自引：0，他引：2  

A M Saad  C Concha  G Astr?m 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(5):337-345

The bovine blood neutrophil phagocytosis and the blood and milk lymphocyte proliferative response upon stimulation with Phytohaemagglutinin, Concanavalin A and Pokeweed mitogens was studied from 3 weeks prior to calving until 3 weeks after calving. Neutrophil phagocytosis and the total and differential blood leukocyte counts were performed by flow cytometry. A gradual increase in the percentage of phagocytized bacteria and the average number of bacteria per phagocyte was observed before calving followed by a sharp fall on the first postpartum. This was followed by a steady increase in the above parameters reaching the highest levels at two weeks postpartum. There was a gradual increase in the number of neutrophils in blood as calving approached followed by a sharp decrease after calving. The number of lymphocytes in blood dropped before calving, being at the lowest level on the day before calving. The proliferative response of blood and milk lymphocytes upon stimulation with the three mitogens was low during the week preceding parturition with the lowest value on the day before calving. The response of blood lymphocytes returned to a higher level the second week after calving while that of milk lymphocytes remained at a low level during the first and the second postpartum weeks.  相似文献   

A survey of peach viruses in Lebanon     

E. Choueiri  C. Haddad  N. Abou Ghanem-Sabanadzovic  F. Jreijiri  S. Issa  A. T. Saad  B. Di  Terlizzi V. Savino 《EPPO Bulletin》2001,31(4):493-497

Field surveys were carried out in the main peach-growing areas of Lebanon to assess the presence and distribution of viruses and viroids in commercial orchards. Field inspections were made in spring and summer 2000 to observe symptoms of virus and viroid diseases respectively. In total, 950 trees in 95 commercial plantings from three different regions of Lebanon (Bekaa Valley, Mount Lebanon and north Lebanon) were surveyed and sampled. Immunoenzymatic tests (DAS-ELISA) were used to ascertain the presence of the following: Prunus necrotic ring spot ilarvirus (PNRSV), Prune dwarf ilarvirus (PDV), Apple mosaic ilarvirus (ApMV), Apple chlorotic leaf spot trichovirus (ACLSV), Plum pox potyvirus (PPV), Tomato ringspot nepovirus (ToRSV) and Strawberry latent ringspot nepovirus (SLRSV). Peach latent mosaic pelamoviroid (PLMVd) and Hop stunt hostuviroid (HSVd) were identified by molecular hybridization. About 25% of the tested samples were infected by one or more viruses. In particular, the prevailing virus was PNRSV (61.2% of infection), followed by ACLSV (27.1%), PDV (22.4%) and ApMV (2.1%). Mixed infections were about 13%. ToRSV, SLRSV and PPV were not found. HSVd was apparently absent, whereas PLMVd was identified in 34% of the samples examined. This viroid prevailed in certain areas of Mount Lebanon in both native and foreign cultivars.  相似文献   

Inclusion of Yucca schidigera extract in diets with different protein levels for dogs          下载免费PDF全文

Jéssica S. Dos Reis  Márcio G. Zangerônimo  Rosana C. S. Ogoshi  Janine França  Adriano C. Costa  Thomás N. Almeida  João P. F. Dos Santos  Carolina P. Pires  Ana F. Chizzotti  Carlos A. L. Leite  Flávia M. O. B. Saad 《Animal Science Journal》2016,87(8):1019-1027

This study evaluated the effects of inclusion of Yucca schidigera extract (YSE) in two diets with different levels of crude protein (CP) for dogs on facal odour, nutrient digestibility, ammonia concentration in feces and hematological and serum biochemical profiles. Twenty adults Beagles were used, distributed in a randomized block design in a 2 × 4 factorial design (two diets, 25% and 34% CP, and four YSE levels: 0, 250, 500 and 750 mg/kg) with five replicates, obtained during two experimental periods. The fecal odour reduced (P < 0.05) when 500 mg/kg of YSE was used in diets with higher CP. The inclusion of YSE reduced (P < 0.05) fecal ammonia, and the inclusion of 250 and 500 mg/kg YSE reduced intestinal gas. The inclusion of 750 mg/kg YSE increased the mean corpuscular hemoglobin (MCH), alanine aminotransferase (ALT) activity and tended to increase the serum cholesterol concentration, regardless of the protein level of the diets. There was no effect on the digestibility of nutrients, fecal consistency, nitrogen balance and thickness of the intestinal wall. The inclusion of 500 mg/kg YSE is effective in reducing fecal odour in dogs receiving diets with 34% of CP. Regardless of the protein content, YSE reduces fecal ammonia, but may cause adverse effects if included at higher doses.  相似文献   

Utilization of Defatted Mango Kernel in Wheat-Based Cereals Products: Nutritional and Functional Properties     

Munazza Shabeer  Muhammad Abrar  M. Suffyan Saddique  Muhammad Imran  Muhammad Saad Hashmi 《International Journal of Fruit Science》2016,16(4):444-460

The mango kernel is a byproduct of mango processing industries and it contains appreciable quantities of edible oil and quality proteins. In the present research, wheat flour was partially replaced with defatted mango kernel flour up to 30% and blends were further utilized for the preparation of biscuits. The nutritional and functional properties of flour blends were determined in the first phase. The second phase dealt with product analysis and sensorial appraisal from the trained taste panel. The results indicated that the addition of mango kernel flour improved the mineral and fiber content of flour blends. The addition of mango kernel flour affected emulsifying and foaming properties of flour blends negatively, while water and oil absorption improved positively from 60.76 ± 2.39% to 86.29 ± 2.51% and 81.81 ± 2.94% to 123.87 ± 5.39%, respectively. The results might be due to lower bulk density of blends. The cookies prepared from flour blends showed improved color tonality and textural characteristics. Sensorial appraisal from a trained taste panel was awarded to cookies containing 15% and 20% defatted mango kernel flour, however, the rating was slightly less than the control. The results were conclusive that defatted mango kernel can be used at 15% and 20% with significant consumer acceptability. Future aspects of the present research include the extraction of principal ingredients, e.g., starch and proteins, for value addition.  相似文献   

Effect of long-term urban wastewater irrigation on heavy metal contamination of soils under conditions of Egypt     

A. V. Shurvilin  N. I. Chernukha  Goma Bothina Saad 《Russian Agricultural Sciences》2010,36(6):452-454

It is established that, with wastewater irrigation of corn on alluvial and gray-brown desert soils, the total content of heavy metals and their mobile compounds increases. However, the content of Zn, Cu, Ni, and Co didn’t exceed the maximum allowable concentration. But Cd and Pb exceeded the maximum allowable concentration during long-term wastewater irrigation of alluvial soils. Irrigation with ground, river, and waste waters for 3 years didn’t lead to considerable heavy metal contamination of soils.  相似文献   

Detection of channel catfish virus in cage‐cultured Pangasius hypophthalmus (Sauvage, 1878) in Malaysia     

A Siti‐Zahrah  M Zamri‐Saad  M Firdaus‐Nawi  M K Hazreen‐Nita  M Nur‐Nazifah 《Journal of fish diseases》2014,37(11):981-983

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