Mutualistic fungus Piriformospora indica modulates cadmium phytoremediation properties of host plant via concerted action of enzymatic and non-enzymatic biochemicals     

Muhammad KHALID  Saeed UR-RAHMAN  Haoxin TAN  Lantian SU  Pei ZHOU  Nan HUI 《土壤圈》2022,32(2):256-267

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Study on Fruitset and Pollen-Compatibility Status in Sweet Cherry (Prunus avuim L.) Cultivars     

Piri  Saeed  Kiani  Esmaiel  Sedaghathoor  Shahram 《Erwerbs-Obstbau》2022,64(2):165-170

Prunus avium L. is one of the most important temperate zone fruits in the world. Most of the cherry cultivars always have difficulties of fertilization and fruit set due to self-incompatibility, so they need suitable and compatible pollinizers for commercial fruit production. In this experiment, pollination compatibility of cherry cultivars of ‘Napoleon’, ‘Burlat’, ‘Zhan’, and ‘Lambert’ was studied as both pollen recipients and donors. To determine the compatibility or incompatibility of pollinizers, percent of fruit set was calculated. This research was carried out as a factorial experiment in a randomized complete block design. The final average fruit set in studied cultivars was different under self or cross-pollination. The results showed that ‘Napoleon’, ‘Zhan’ and ‘Lambert’ cultivars are the suitable pollinizers for ‘Burlat’. Also, ‘Burlat’ is a cultivar which produced many fruits under self-pollination. Therefore, ‘Burlat’ can be used as monoculture for orchard establishment. Results showed that cultivar ‘Napoleon’ was cross-incompatible with ‘Lambert’. ‘Zhan’, ‘Napoleon’ and ‘Lambert’ cultivars are founded self-incompatible and require cross-pollinations to obtain fruits.

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A Single Point Mutation within the Coding Sequence of Cholera Toxin B Subunit Will Increase Its Expression Yield     

Bita Bakhshi  Mina Boustanshenas  Masoud Ghorbani 《Iranian Biomedical Journal》2014,18(3):130-135

Background: Cholera toxin B subunit (CTB) has been extensively considered as an immunogenic and adjuvant protein, but its yield of expression is not satisfactory in many studies. The aim of this study was to compare the expression of native and mutant recombinant CTB (rCTB) in pQE vector. Methods: ctxB fragment from Vibrio cholerae O₁ ATCC14035 containing the substitution of mutant ctxB for amino acid S128T was amplified by PCR and cloned in pGETM-T easy vector. It was then transformed to E. coli Top 10F'' and cultured on LB agar plate containing ampicillin. Sequence analysis confirmed the mature ctxB gene sequence and the mutant one in both constructs which were further subcloned to pQE-30 vector. Both constructs were subsequently transformed to E. coli M15 (pREP4) for expression of mature and mutant rCTB. Results: SDS-PAGE analysis showed the maximum expression of rCTB in both systems at 5 hours after induction and Western-blot analysis confirmed the presence of rCTB in blotting membranes. The expression of mutant rCTB was much higher than mature rCTB, which may be the result of serine-to-threonine substitution at position 128 of mature rCTB amino acid sequence created by PCR mutagenesis. The mutant rCTB retained pentameric stability and its ability to bind to anti- cholera toxin IgG antibodies. Conclusion: Point mutation in ctxB sequence resulted in over-expression of rCTB, probably due to the increase of solubility of produced rCTB. Consequently, this expression system can be used to produce rCTB in high yield. Key Words: Escherichia coli, Point mutation, Cholera toxin B subunit (CTB), Protein expression  相似文献   

Shift work as an oxidative stressor     

Akbar?SharifianEmail author  Saeed?Farahani  Parvin?Pasalar  Marjan?Gharavi  Omid?Aminian 《Journal of circadian rhythms》2005,3(1):15

Background  

Some medical disorders have higher prevalence in shift workers than others. This study was designed to evaluate the effect of night-shift-working on total plasma antioxidant capacity, with respect to the causative role of oxidative stress in induction of some of these disorders.  相似文献   

EXOGENOUS APPLICATION OF POTASSIUM DIHYDROGEN PHOSPHATE CAN ALLEVIATE THE ADVERSE EFFECTS OF SALT STRESS ON SUNFLOWER     

Muhammad Saeed Akram  Muhammad Ashraf 《Journal of plant nutrition》2013,36(7):1041-1057

A greenhouse experiment was conducted to examine whether foliarly applied potassium + phosphorus (K + P) in the form of monopotassium phosphate (KH₂PO₄) could mitigate the adverse effects of salt stress on sunflower plants. There were two levels of root-applied salt [0 and 150 mM of sodium chloride (NaCl)], and varying levels of KH₂PO₄ [(NS (no spray), WS (spray of water), 5 + 4, 10 + 8, 15 + 12, and 20 + 16 mg g^?1 K + P, pH 6.5] applied foliarly to 18-day old non-stressed and salt stressed sunflower plants. Salt stress adversely affected the growth, yield, photosynthetic capacity, and accumulation of mineral nutrients in the sunflower plants. However, varying levels of foliar applied KH₂PO₄ proved to be effective in improving growth and yield of sunflower under salt stress. The KH₂PO₄ induced growth in sunflower was found to be associated with enhanced photosynthetic capacity, water use efficiency and relative water contents.  相似文献   

Accumulative effects of humic acid and multinutrient foliar fertilizers on the vegetative and reproductive attributes of citrus (Citrus reticulata cv. kinnow mandarin)     

Amir Hameed  Shabih Fatma  Javed Iqbal Wattoo  Muhammad Yaseen  Saeed Ahmad 《Journal of plant nutrition》2013,36(19):2495-2506

Abstract

Field trials were performed to investigate the effects of humic acid (HA) and multinutrient foliar fertilizer “Micro Power” (MP) coupled with farmer’s practices ( FP ), addressed in single and/or split dose frames at different plant phenological stages on various vegetative, reproductive, and physiological attributes of citrus trees (Citrus reticulata cv. kinnow mandarin). The results exhibited a profound response of treatments on various growth parameters (32.5% increase in plant height, 22.2% increase in fruit set branch^?1, 5.25% decrease in fruit drop percentage, 89.81% increase in fruit yield (kg), etc.) of citrus trees when compared to the control (FP). Likewise, a significant positive response was observed regarding various plant physiological parameters (leaf nutrients, total chlorophyll content, etc.) and physicochemical characteristics (ascorbic acid, total sugars, etc.) of citrus fruits. This study confirmed the reproducibility of HA and MP applications to improve the yield/quality of citrus and can lead to an organically sustainable citriculture.  相似文献   

The antimicrobial peptide AsR416 can inhibit the growth,sclerotium formation and virulence of Rhizoctonia solani AG1-IA     

Nassimi  Zohreh  Taheri  Parissa  Kong  Xi  Dong  Wubei  Tarighi  Saeed 《European journal of plant pathology / European Foundation for Plant Pathology》2021,160(2):469-485

European Journal of Plant Pathology - Plant antimicrobial peptides (AMPs) as a part of plant defense responses, are small soluble defense molecules which can inhibit the growth of pathogens. This...  相似文献   

Effect of Fe,Zn and Cu on quantity and quality characteristics and nutrient accumulation in wheat     

Sharafi  Saeed  Sharifdost  Fatemeh  Mohajeri  Fatemeh 《Journal of Crop Science and Biotechnology》2021,24(4):469-476

Journal of Crop Science and Biotechnology - Wheat is the most important food on earth. Therefore, to produce high-quality products with efficient utilization, the micronutrient groups are needed....  相似文献   

In vitro dephytinization and bioavailability of essential minerals in several wheat varieties     

Sharoon Akhter  Asma Saeed  Muhammad Irfan  Kauser Abdullah Malik 《Journal of Cereal Science》2012

Twelve wheat varieties were studied for their phytic acid, calcium, iron, zinc, copper and phosphorus contents, which respectively ranged between 114–166, 25.1–53.5, 3.41–5.55, 0.71–3.00, 0.65–1.32 and 298–314 mg/100 g. The molar ratios as Ca:Phy, Phy:Fe, Phy:Zn, Phy:Cu were found to be 0.14–0.29, 1.96–3.86, 5.11–20.5 and 13.0–23.9, respectively. Significant dephytinization, ranging between 35.3 and 69.3% in different varieties, was achieved on exogenous enzymatic treatment. Enzymatic dephytinization in the in vitro simulated gastrointestinal digestion resulted in significant increase in the bioavailability of essential minerals. The increase in bioavailability of calcium, iron, zinc and copper on exogenous enzymatic dephytinization ranged between, respectively (x-folds): 1.30–1.96, 1.11–1.52, 1.22–1.78 and 1.11–1.73.  相似文献   

Short-Term Storage of Semen of Rainbow Trout: Interactions of Time,Antibiotic, and Activator     

Hamid Niksirat  Koorosh Sarvi  Asghar Abdoli  Saeed Hajirezaee 《Journal Of Applied Aquaculture》2013,25(4):358-366

This study was designed to examine the main effects and interactions of time, presence of antibiotics, and type of sperm activators on the fertilization capacity (eyeing rate) of refrigerated semen of rainbow trout, Oncorhynchus mykiss. The semen samples were stored in the presence or absence of 250 IU ml^?1 penicillin and 250 μg ml^?1 streptomycin sulfate. Freshwater and a saline solution were used as sperm activators. The semen samples were stored at 2–3°C and fertilized after 0, 6, 8, 12, 19, and 25 days of storage. Fertilizing capacities of semen samples stored in the presence of antibiotics (63.8 ± 5.6%) were significantly (p < 0.05) higher than those stored in the absence of antibiotics (46.2 ± 6.7%). Also, the fertilizing capacities of stored semen samples activated using saline solution (70.7 ± 5.7%) had significantly (p < 0.05) higher values than those activated using freshwater (39.3 ± 5.9%). Semen samples stored in the absence of antibiotics completely lost fertilizing capacity within 19 days of storage. After 25 days of storage in the presence of antibiotics, induction of fertilization using freshwater and saline solution resulted in 0% and 79.8 ± 1.7% fertility, respectively.  相似文献