Virulence gene and antibiotic resistance profile of Shiga-toxin-producing Escherichia coli prevalent in captive yaks (Poephagus grunniens)   总被引：2，自引：0，他引：2  

Samiran Bandyopadhyay  Tapas K. Biswas  Devasis Sasmal  Monoj K. Ghosh  Tapan K. Dutta  Suresh C. Das  Debasis Bhattacharya  Asit K. Bera  Subhasis Bandyopadhyay  Sumanta De  Diganta Pan 《Veterinary microbiology》2009,138(3-4):403-404

  相似文献   

Amelioration of altered oxidant/antioxidant balance of Indian water buffaloes with subclinical mastitis by vitamins A, D3, E, and H supplementation     

Umesh Dimri  Mahesh Chandra Sharma  Shanker K. Singh  Pankaj Kumar  Ricky Jhambh  Bishwambhar Singh  Samiran Bandhyopadhyay  Med Ram Verma 《Tropical animal health and production》2013,45(4):971-978

The effect of vitamins A, D₃, E, and H supplementation on oxidative stress indices in Indian water buffaloes suffering from subclinical mastitis was investigated. Changes in the total oxidant capacity (TOC), total antioxidant capacity (TAC), nitric oxide (NO), and activities of glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) in milk were evaluated before and after the supplementation of vitamins A, D₃, E, and H. The buffaloes suffering from subclinical mastitis revealed remarkable alterations in the milk oxidants/antioxidants balance shifted towards oxidative status. The buffaloes with subclinical mastitis revealed significantly (P?≤?0.01) higher TOC, NO contents, and CAT activity, while TAC content and GSH-Px activity were significantly (P?≤?0.01) lower in comparison with the healthy controls. However, SOD activity did not show any significant change. Supplementation of vitamins A, D₃, E, and H to these animals revealed significant (P?≤?0.01) reduction in TOC, NO, and CAT, while a significant (P?≤?0.01) increase in TAC and GSH-Px activity was also evident. From the present study, it may be concluded that supplementation of these vitamins can help ameliorate the altered milk oxidants/antioxidants balance towards normalcy and, thus, ensue recovery from subclinical mastitis in the Indian water buffaloes.  相似文献   

Effect of environmental temperature on quality and developmental competence in vitro of buffalo oocytes     

Nandi S  Chauhan MS  Palta P 《The Veterinary record》2001,148(9):278-279

  相似文献   

Effect of insulin,transferrin and selenium and epidermal growth factor on development of buffalo oocytes to the blastocyst stage in vitro in serum-free,semidefined media     

Raghu HM  Nandi S  Reddy SM 《The Veterinary record》2002,151(9):260-265

The in vitro development of buffalo oocytes up to the blastocyst stage was studied in serum-free, semidefined media containing bovine serum albumin, follicle-stimulating hormone (FSH), insulin, transferrin and selenium (ITS) and epidermal growth factor (EGF). In experiment 1, oocytes aspirated from abattoir-derived ovaries were cultured in eight serum-free, semidefined culture media containing different combinations of these four factors. In experiment 2, the maturation of buffalo oocytes and the development of the embryos were compared in a complex co-culture system and in the serum-free, semidefined media. Supplementation with FSH and EGF significantly (P < 0.05) increased the maturation rates of buffalo oocytes, and the yield of blastocysts was higher (P < 0.05) in media containing EGF and ITS. The yield of blastocysts was lower in the serum-free semidefined media (P < 0.05) than in the complex co-culture system.  相似文献   

Co‐culture of Buffalo Preantral Follicles with Different Somatic Cells     

HS Ramesh  PSP Gupta  S Nandi  BM Manjunatha  V Girish Kumar  JP Ravindra 《Reproduction in domestic animals》2008,43(5):520-524

The effect of co‐culture of buffalo preantral follicles (PFs) with different somatic cells, i.e, cumulus, granulosa, ovarian mesenchymal and oviductal epithelial cells was studied. Large PFs (250–450 μm) were isolated by microdissecting the trypsin (1%) digested ovarian cortical slices. Cumulus cells were isolated by repeated pipetting of oocytes, granulosa cells were isolated by aspirating from punctured PFs and ovarian mesenchymal cells were isolated from ovarian cortex by scraping the cortical slices and passing through 20 μm filter. Preantral follicles were cultured in standard culture medium without somatic cells or co‐cultured with cumulus cells, granulosa cells, ovarian mesenchymal cells and oviductal epithelial cells for 80 days. The growth rate (μm/day) of the PFs was monitored by measuring follicular diameter on day 0, 30, 60 and 80 days of culture. The viability of PFs was evaluated by trypan blue staining. The results indicated that PFs co‐cultured with cumulus, granulosa and ovarian mesenchymal cells had a better development and survivality compared with control and those co‐culture with oviductal epithelial cells. Maximum growth and survivality of PFs were achieved when cultured with cumulus cells. It is concluded that inclusion of somatic cells in PF culture media had beneficial effect on the growth of PFs and cumulus cells supported maximum growth and survivality of PFs in vitro of all somatic cells tested.  相似文献   

Changes in plasma hormones profile and liver function in cows naturally exposed to lead and cadmium around different industrial areas     

Swarup D  Naresh R  Varshney VP  Balagangatharathilagar M  Kumar P  Nandi D  Patra RC 《Research in veterinary science》2007,82(1):16-21

The present study was carried out to assess the endocrine status and liver function in adult cows reared in polluted environment around different industrial units in India.The effect on endocrine system was examined by determination of plasma level of thyroid hormones, thyroxin (T4) (n=269) and triidothyronin (T3) (n=269), stress hormone cortisol (n=266), and reproductive hormones such as estradiol (n=84) and progesterone (n=84) in cows (>3 years) reared around different polluted industrial and non-polluted areas.The respective blood lead and cadmium concentration was also determined in all the cows.The mean plasma levels of both T3 and T4 were significantly (P<0.05) higher around lead zinc smelter (2.43+/-0.26 and 41.1+/-2.9nmol/L) and closed lead cum operational zinc smelter (1.81+/-0.16 and 42.4+/-6.2nmol/L), where the mean blood lead level (0.86+/-0.06 and 0.51+/-0.09mug/ml) was also significantly higher than that of cows (0.07+/-0.01mug/ml) from unpolluted areas.Regression analysis of data from 269 cows revealed a significant (P<0.01) positive correlation between the blood lead and plasma T3 (r=0.287) and T4 (r=0.173).The correlation between thyroidal hormones and the blood cadmium concentration (r=-0.079 and -0.48; P>0.05) was not significant.Plasma cortisol level had also a non-significant (P>0.05) correlation (r=-0.092) with blood lead level.However, the mean cortisol level (4.02+/-1.96nmol/L) of cows in phosphate rock mining areas was significantly (P<0.05) higher than that of controls (1.98+/-0.70nmol/L). The mean plasma estradiol level was significantly (P<0.05) higher in cows around closed lead cum operational zinc smelter (47.1+/-19.5pg/ml) than that of the control animals (21.8+/-3.9pg/ml) and in rest of the areas, the difference did not reach the statistical significance (P>0.05).The serum biochemical analysis in 36 cows around lead-zinc smelter with the highest mean blood lead level (0.86+/-0.06mug/ml) amongst all the industrial/urban areas surveyed, and in 15 animals from non-polluted areas revealed a significant positive correlation between blood lead and serum ALT (alanine transaminase) (r=0.688, P<0.01) and AST (aspartate transaminase) (r=0.390, P<0.01) and a negative correlation with serum total lipids (r=-0.337, P<0.05), total protein (r=-0.449, P<0.01) and albumin(r=-0.662, P<0.01). It is concluded from the study that the natural exposure to lead in polluted environments disturbs the endocrine profile and the higher blood lead level alters serum biochemical parameters indicative of liver functions.  相似文献   

Serological evidence of bovine herpesvirus-1 (BoHV-1) infection in yaks (<Emphasis Type="Italic">Peophagus grunniens</Emphasis>) from the National Research Centre on Yak,India     

Sukdeb Nandi  Manoj Kumar 《Tropical animal health and production》2010,42(6):1041-1042

Bovine herpesvirus-1 (BoHV-1) causes a variety of disease syndromes including, respiratory, nervous and reproductive disorders both in domestic as well as wild bovines and the disease is prevalent throughout the world including India. In this study, serum samples of yaks were screened for serological evidence of BoHV-1 in a yak farm with history of abortion and kerato-conjuctivitis by competition-ELISA. The result of seroprevalence of BoHV-1 infections in yaks (Poephagus grunniens) revealed that the overall seroprevalence was 60.1%. The sero-prevalence of BoHV-1 infections was highest in male calf (67.7%) followed by yak cows (62.6%), yak bulls (56.8%), and yak heifers (50.0%).  相似文献   

Antisperm antibodies in repeat‐breeding cows: Frequency,detection and validation of threshold levels employing sperm immobilization,sperm agglutination and immunoperoxidase assay          下载免费PDF全文

SK Srivastava  S Shinde  SK Singh  S Mehrotra  MR Verma  AK Singh  S Nandi  N Srivastava  SK Singh  TK Goswami  SK Bhure  H Kumar  SK Ghosh 《Reproduction in domestic animals》2017,52(2):195-202

Antisperm antibodies have been found in repeat‐breeding(RB) cows, and those causing agglutination and/or immobilization of sperm are considered to be closely related to unexplained infertility. However, a standard protocol for identifying antisperm antibodies (ASA) in cattle is not validated. Therefore, an investigation was undertaken to evaluate sperm immobilization (SIT), sperm agglutination (SAT) and immunoperoxidase (IPT)assays for detection of ASA in serum and their respective threshold levels for confirmation. Animals (heifers, normally breeding, repeat‐breeding and pregnant animals) that were free from IBR, brucellosis and uterine infections (screened by clinical examination) were included in the study. Sperm agglutinating, sperm immobilizing and antisperm antibodies evaluated by respective assay were significantly higher (p < .05) in RB cows compared to other groups. The SIT assay was able to identify 61% of RB caused by ASA, more than those employing SAT and IPT. Furthermore, a dilution rate of 1:5 and 1:80 (confirms 59.0 and 57.0% RB+ve)were sufficient to diagnose ASA by SAT and IPT, respectively. Results indicate the presence of __12.6% clumped spermatozoa and __ 2.6%(cut‐off value) peroxidase‐positive spermatozoa at 1:5 and 1:80 dilutions diagnosed with SAT and IPT, respectively, may be considered as repeaters arising out of ASA. Furthermore, study also showed the presence of lower incidence of ASA positivity in other groups of animals (heifer<normal breeder<pregnant animals) compared to repeaters. Study results show that although IPT is more specific and accurate but SAT and SIT are comparatively simple and cost‐effective assays suitable for detecting ASA under field conditions and thus can be recommended for screening of repeaters.  相似文献   

The fission yeast FANCM ortholog directs non-crossover recombination during meiosis     

Lorenz A  Osman F  Sun W  Nandi S  Steinacher R  Whitby MC 《Science (New York, N.Y.)》2012,336(6088):1585-1588

The formation of healthy gametes depends on programmed DNA double-strand breaks (DSBs), which are each repaired as a crossover (CO) or non-crossover (NCO) from a homologous template. Although most of these DSBs are repaired without giving COs, little is known about the genetic requirements of NCO-specific recombination. We show that Fml1, the Fanconi anemia complementation group M (FANCM)-ortholog of Schizosaccharomyces pombe, directs the formation of NCOs during meiosis in competition with the Mus81-dependent pro-CO pathway. We also define the Rad51/Dmc1-mediator Swi5-Sfr1 as a major determinant in biasing the recombination process in favor of Mus81, to ensure the appropriate amount of COs to guide meiotic chromosome segregation. The conservation of these proteins from yeast to humans suggests that this interplay may be a general feature of meiotic recombination.  相似文献   

The cost of access to HIV treatment     

Galatowitsch P  Siegfried N 《Science (New York, N.Y.)》2006,314(5805):1540-1; author reply 1540-1

  相似文献